Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL68 pRS314-GPDp-Erg6-V5-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: lipid droplets.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18574_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB18574_B0J5a.seq
>D07393A8_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNAACGCC NGGGTTTTCC CAGTCACGAC    61 GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCA   121 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT   181 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC   241 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT   301 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA   361 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT   421 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCAATG   481 GAGTGATGCA ACCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC   541 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGATTTGGA AAAAGCTGAA   601 AAAAAAGGTT GAAACCAGTT CCCTGAAATT ATTCCCCTAC TTGACTAATA AGTATATAAA   661 GACGGTAGGT ATTGATTGTA ATTCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT   721 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC NGANGCGGCC   781 GCNTGANTGA AACAGAATTG AGAAAAAGAC AGGNCCNATT CACTANGGAG TTACATGGTG   841 ATGATATTGG TAAAAAGANN GGNTTGANNG CATTGATGTC GAANNAACAA CTCTGCNCNA   901 ANNNNANCCG TNNNNAANTA CTTGANAAAT TGGGNNGGNA GAANCGATNA ANANGCCNNA   961 NAANNNCNTC NTGNNNNANT ATANNGNNNN CNCNCNNTNC CTACNNNNAN NNNNTTNNNN 1021 ANNNNNNNNN N //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3') Sequence file: RDB18574_B0J5b.seq
>D07393A8_B0J5_3_SV40A3'ElementReverse_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGN NNAANNCNNG CAACGCGGCC TTTTTACGGT    61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG   121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG   181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC   241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG   301 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC   361 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG   421 GAAACAGCTA TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA   481 AGCTGGTACC GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT   541 TTCAGTATAA TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT   601 ATAAATAACG TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA   661 GGTTGTCTAA CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG   721 CGTGACATAA CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT   781 CTTANGTAAT GCCTGCAGCA TTGACATATT CGTGTAGAAN CATATGATCT CTGCTTGTGG   841 AACCNCCACC ACTTCCNCCA GTTGAGTCTA AGCCCNAAAN ANGGTTNNGT ATGGGTTTCC   901 CANAANCCTC CGGCNNANGN NCCTNGANTT GCTNNTTGGG AANTTTGGGA NGGGGNTNCG   961 GNGTTTTCTG GNNTNNNNAN CNNNNAAAAG CATCATNNGN NNNGNNNNAN NTTNGNNNNN 1021 NNNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18574_B0J5c.seq
>D07393A8_B0J5_3_TRP1pro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNAGGCAGCT TGGANTANGT CTGTTATTAA TTTCNCAGGT    61 AGTTCTGGTC CNTTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT   121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT   181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC   241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG   301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC   361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA   421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA   481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG   541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT   601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT   661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT   721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG   781 CACAGATGCG TAAGGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTAAT ATTTTGTTAA   841 AATTCGCGTT AAATTTTTGT NNAATCAGCT CATTTTTTAA CCAATANGCC GAAATCGGCA   901 AAATCCCTTA TAAATCNAAA GAATANANCG ANATAGGGTT GANTGNTGNT CCNGTTTGGN   961 ANNAGAGTNN NCTATTAAAG AACGNGNNNN NNNNCNNNNA NNNNNAAAAA NCGNCTATCN 1021 NNNNNNNGNN NNNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, V5-GFP11, Erg6 3') Sequence file: RDB18574_B0J5d.seq
>D07393A8_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GCGGNNGNGG GGGGAGGGCG TGAATGTAAG CGTGACATAA    61 CTAATTACAT GACTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTTAGGTAAT   121 GCCTGCAGCA TTGACATATT CGTGTAGAAC CATATGATCT CTGCTTGTGG AACCACCACC   181 ACTTCCACCA GTTGAGTCTA AGCCCAAAAG AGGGTTTGGT ATGGGTTTCC CAGAACCTCC   241 GGCAGAGGAT CCTTGAGTTG CTTCTTGGGA AGTTTGGGAG GGGGTTTCGG CGTTTTCTGG   301 CTTCCTAGCG ACGAAAAGCA TCATTGGAGT GAATAACTTG GACTTACCAC CGGCAACTAA   361 ACCAACCGCA GCATTTTCTA GAGCAGCAGT AACTTCCTTG GAACCTTCTG GGGCTAGACC   421 TAATTTTTCC ATTACAGTAA CCATTGCTGT AGTAAATTGT CTACCCAAGT AAGAAGTTCT   481 GAAAAATGTG GCCAAATTAG CTAAGTTTTG AACGTACTTC CACTCACCAG TTAATGGGTA   541 ATACCAAGGG ATTTCATCAT CATTGTCCGC CAGGTCTTCG CTAACGAGGA CTTCGAAACC   601 ACAGTTCTTC AATGCTTTCC TAGCCACGTC GACATGGAAC ATCTTTGGGA TACCATCACC   661 TAGTTCAATT TCATAAGCGA TCTTTCTATG TTCAGGATTG TTTTCGTCAT ATTTATCAGT   721 CATTACCCAT TCGTAAACAG CAAAGGTANC ACCCGGTTTC AAAACCTTGT AGATTTCGCT   781 GTATACACCT TCTAATTTTG GAGCGTGACA TGTGGCCTCA ATTGCATAAA CTTTGTCNAA   841 AGTGTTTTCT TCGAAATCCA TTTTCNTGAA ATCACCCTTT ACAAAGTCCA TTTGGTCANT   901 CAAATTGTAT TTTTTTANCG NAATATTTTG NCNNNNCAAT TTGGNAATCG TTATTGNTTA   961 NACNNATGAN GTTNCANNCN GNNAATNNNG NNNTCTNNNT NGNTGGGNCC NNNNCACNNN 1021 NNNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Erg6 5') Sequence file: RDB18574_B0J5e.seq
>D07393A8_B0J5_3_TDH3pro_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTGNNNTTCN GNAAATCTAT TTCTTAAACT TCTTAAATTC    61 TACTTTTATA GTTAGTCTNT NNTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG   121 CGGCCGCATG AGTGAAACAG AATTGAGAAA AAGACAGGCC CAATTCACTA GGGAGTTACA   181 TGGTGATGAT ATTGGTAAAA AGACAGGTTT GAGTGCATTG ATGTCGAAGA ACAACTCTGC   241 CCAAAAGGAA GCCGTTCAGA AGTACTTGAG AAATTGGGAT GGTAGAACCG ATAAAGATGC   301 CGAAGAACGT CGTCTTGAGG ATTATAATGA AGCCACACAT TCCTACTATA ACGTCGTTAC   361 AGATTTCTAT GAATATGGTT GGGGTTCCTC TTTCCATTTC AGCAGATTTT ATAAAGGTGA   421 GAGTTTCGCT GCCTCGATAG CAAGACATGA ACATTATTTA GCTTACAAGG CTGGTATTCA   481 AAGAGGCGAT TTAGTTCTCG ACGTTGGTTG TGGTGTTGGG GGCCCAGCAA GAGAGATTGC   541 AAGATTTACC GGTTGTAACG TCATCGGTCT AAACAATAAC GATTACCAAA TTGCCAAGGC   601 AAAATATTAC GCTAAAAAAT ACAATTTGAG TGACCAAATG GACTTTGTAA AGGGTGATTT   661 CATGAAAATG GATTTCGAAG AAAACACTTT CGACAAAGTT TATGCAATTG AGGCCACATG   721 TCACGCTCCA AAATTAGAAG GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGNGG   781 TACCTTTGCT GTTTACGANT GGGTAATGAC TGATAAATAT GACGAAAACA ATCCTGAACA   841 TAGAAAGATC GCTTATGAAA TTGANCTANG TGANGGTATC CCNAAGANGT TNCANGTCNA   901 CGNGGCTAGG AAAGCATTGA AGAANTGTGN TTTCNAAGTC CTCGTTAGCG ANNNNNNNNN 961 N //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18574_B0J5f.seq
>D07393A8_B0J5_3_Amp_R_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNATTTATCN GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA   781 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG   841 GGGCTGGCTT AACTATGCGG CATCNNANCA GANTGNACTG AGANNGNNCC NTANATCACG   901 ANATTACTAT ATATATAATA TAGGAAGCAT TTAATANAAN NGCATCGTAA TATATGTGTA   961 CTTTGCAGTT ATGANNNNNN ANGNNANNAN NGNNNNANAN NCNTTANNGN AAAATANNNN 1021 GNNNCNNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl