The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: endoplasmic reticulum.
Alternative name | pSFL66, pRS314-GPDp-Ifa38-3xFLAG-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Ampicillin (E. coli), TRP1 (S. cerevisiae). |
Gene/insert name | S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18573 | pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 | DNA solution |
Materials & Methods section:
The pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18573). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18573_B0J5p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18573_B0J5a.seq ![]() |
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>D07393A7_B0J5_1_HB-NOS_R_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT AANGCCNGGG TTTTCCCNGT CACGACGTTG 61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT 121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC 181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA 241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC 301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA 361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA 421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT 481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC 541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA 601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG 661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT 721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT 781 GACTTTTATG CAACAGCTTC NAGANGCTGG GGAAAGANTT ANGTGTATCA ATGGTCTTTT 841 ATGGGTTGTT TTTGGGCTTG GNGTCTTAAA ATGTACAACG TTATCACTTA NATTTTTTAG 901 CTCTNATTTT TGATCTTTTT TTTACTACCA GCAGTCAATT TNCGANAAGT ATNGNNGCTA 961 AAACTGGNNA AATACTGNGC TATCACNNGN NGNNNGNGNN NGNNNTNNNN NNNNNN // |
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB18573_B0J5b.seq ![]() |
>D07393A7_B0J5_1_SV40pA3'ElementReverse_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNAN GNNNNNNGCN NGCANCGCGG CCTTTTTACG GTTCCTGGCC 61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA 481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT 541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA 601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT 661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT 721 AACTAATTAC ATGACTCGAG GTCGACNGTA TCGATAAGCT TGATATCGAA TTCTTATGTT 781 ATCCCTGCAG CATTGACGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACNTCCA 841 CCACTTCNGC CNTTATCGNC ATCATCCTTA TAGNCNATGT CATGATCCTT ATAATCGCCN 901 TCATGGNCTT TGTAATCNCC ACNNGCAGAN GANNNNTCCN TTTTNNNNNN NNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Ifa38 3') Sequence file: RDB18573_B0J5c.seq ![]() |
>D07393A7_B0J5_1_CYC1ter_R_H02_23_ABI24.ab1 1 NNNNNNNNNN NGNNNNAGCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA 61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC 121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT 181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG 241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT 301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA 361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT 421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT 481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT 541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC 601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG 661 GGTGGGAATC AGACCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT 721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA 781 TGTAGCAGTG TTATTGATAG TGATAATATT TCTAAGCTCC TTCTCTTCTG TTTCCAAAAA 841 TGGAACAGGA ATGGAGTGTG ATTGANNAAC ATTATTGANN NAAACGGNGA NTGGTAACTG 901 CGCACANAAT TCCTNNANGN ANTCATAGTT GGANTCNTTA NNCNCCNNNN NGTCNANNGN 961 NANAATCTTT ANNNCGANNT GATGNNGNNN NNCNANNTNN TTTTNNNNAN NNCNNNNNNN 1021 TNNNNNNNNN N // |
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18573_B0J5d.seq ![]() |
>D07393A7_B0J5_1_TRP1pro_F_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCT NGGNNTNNGT CTGTTATTAA TTTCNCAGGT 61 AGTTCTGGTC CATTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT 121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT 181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC 241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG 301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC 361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA 421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA 481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG 541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT 601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT 661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT 721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG 781 CACAGATGCG TAANGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTNAT ATTTTGTTAA 841 AATTCGCGTT NAATTTTTGT TAAATCAGCT CATTTTTTAA CCNATANNNC GAAATCGGCA 901 AAATCCNNTA TAANTCAAAA GAATAGACCG ANATANGGNT NNANTGNTGN TNNCNNTTTG 961 GNANCNNGAN NNCNCNANTN NNNNNNNNGN NNTNNNNNNN NNANNGNNNN NANNNNNNNN 1021 NN // |
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18573_B0J5e.seq ![]() |
>D07393A7_B0J5_1_TDH3pro_F_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN TGNNNTTCTG NNNNCTATTT CTTAAACTTC TTAAATTCTA 61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG 121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG 181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT 241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG 301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG 361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT 421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA 481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT 541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT 601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT 661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT 721 CGGGTACTCG TGGTTTGATC TTAACCNTGG GCTCATTTGG TGGTCTGATT CCCACCCCAC 781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACNAGG TTGGTCTAAC TCTTTGGCTG 841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTTCATATTT GGNCACTANC 901 TCGANNNCTA AAATCANAAG ATCATCNTTT NNNGANNCNN AATNCNCNNN ANNTNGTNAA 961 NNNNNNNTNN ANNNNNNNNN N // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18573_B0J5f.seq ![]() |
>D07393A7_B0J5_1_Amp_R_H03_24_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTGA NCATTTATNN GGGTTATTGT CTCATGAGCG GATACATATT 61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA 181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT 241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT 301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG 361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC 421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT 481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT 541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT 601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA 781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGTGTCG 841 GGGCTGGNTT AACTATGCGG CATCNNANCA GANTGTACTG ANAGTGNNNC ATANATCACG 901 ACATTACTAT ATATATAATA TANGAAGCNT TTAATAGAAC AGCATCGNAA TATATGTGNA 961 CTTTGCANNN ATGANGCCNN ANGNNANNAN NNNNNNANNN TCTTNNNNNN AAAANNANCN 1021 TNNNNNNNNT NNNNNNNNNN // |
Primer: Sequence file: RDB18573_B0J5g.seq ![]() |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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