Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 (#RDB18573)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: endoplasmic reticulum.

Alternative name	pSFL66, pRS314-GPDp-Ifa38-3xFLAG-GFP11
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pRS314 (plasmid)
Selectable markers	Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name	S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer	Tamura, Yasushi |

 

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18573	pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18573).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18573_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18573_B0J5a.seq
>D07393A7_B0J5_1_HB-NOS_R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT AANGCCNGGG TTTTCCCNGT CACGACGTTG    61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT   121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC   181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA   241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC   301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA   361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA   421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT   481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC   541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA   601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG   661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT   721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT   781 GACTTTTATG CAACAGCTTC NAGANGCTGG GGAAAGANTT ANGTGTATCA ATGGTCTTTT   841 ATGGGTTGTT TTTGGGCTTG GNGTCTTAAA ATGTACAACG TTATCACTTA NATTTTTTAG   901 CTCTNATTTT TGATCTTTTT TTTACTACCA GCAGTCAATT TNCGANAAGT ATNGNNGCTA 961 AAACTGGNNA AATACTGNGC TATCACNNGN NGNNNGNGNN NGNNNTNNNN NNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB18573_B0J5b.seq
>D07393A7_B0J5_1_SV40pA3'ElementReverse_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN GNNNNNNGCN NGCANCGCGG CCTTTTTACG GTTCCTGGCC    61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC   121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC   181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT   241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG   301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG   361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC   421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA   481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT   541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA   601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT   661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT   721 AACTAATTAC ATGACTCGAG GTCGACNGTA TCGATAAGCT TGATATCGAA TTCTTATGTT   781 ATCCCTGCAG CATTGACGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACNTCCA   841 CCACTTCNGC CNTTATCGNC ATCATCCTTA TAGNCNATGT CATGATCCTT ATAATCGCCN 901 TCATGGNCTT TGTAATCNCC ACNNGCAGAN GANNNNTCCN TTTTNNNNNN NNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Ifa38 3') Sequence file: RDB18573_B0J5c.seq
>D07393A7_B0J5_1_CYC1ter_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NGNNNNAGCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA    61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC   121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT   181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG   241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT   301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA   361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT   421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT   481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT   541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC   601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG   661 GGTGGGAATC AGACCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT   721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA   781 TGTAGCAGTG TTATTGATAG TGATAATATT TCTAAGCTCC TTCTCTTCTG TTTCCAAAAA   841 TGGAACAGGA ATGGAGTGTG ATTGANNAAC ATTATTGANN NAAACGGNGA NTGGTAACTG   901 CGCACANAAT TCCTNNANGN ANTCATAGTT GGANTCNTTA NNCNCCNNNN NGTCNANNGN   961 NANAATCTTT ANNNCGANNT GATGNNGNNN NNCNANNTNN TTTTNNNNAN NNCNNNNNNN 1021 TNNNNNNNNN N //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18573_B0J5d.seq
>D07393A7_B0J5_1_TRP1pro_F_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCT NGGNNTNNGT CTGTTATTAA TTTCNCAGGT    61 AGTTCTGGTC CATTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT   121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT   181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC   241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG   301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC   361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA   421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA   481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG   541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT   601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT   661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT   721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG   781 CACAGATGCG TAANGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTNAT ATTTTGTTAA   841 AATTCGCGTT NAATTTTTGT TAAATCAGCT CATTTTTTAA CCNATANNNC GAAATCGGCA   901 AAATCCNNTA TAANTCAAAA GAATAGACCG ANATANGGNT NNANTGNTGN TNNCNNTTTG   961 GNANCNNGAN NNCNCNANTN NNNNNNNNGN NNTNNNNNNN NNANNGNNNN NANNNNNNNN 1021 NN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18573_B0J5e.seq
>D07393A7_B0J5_1_TDH3pro_F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TGNNNTTCTG NNNNCTATTT CTTAAACTTC TTAAATTCTA    61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG   121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG   181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT   241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG   301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG   361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT   421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA   481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT   541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT   601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT   661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT   721 CGGGTACTCG TGGTTTGATC TTAACCNTGG GCTCATTTGG TGGTCTGATT CCCACCCCAC   781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACNAGG TTGGTCTAAC TCTTTGGCTG   841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTTCATATTT GGNCACTANC   901 TCGANNNCTA AAATCANAAG ATCATCNTTT NNNGANNCNN AATNCNCNNN ANNTNGTNAA 961 NNNNNNNTNN ANNNNNNNNN N //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18573_B0J5f.seq
>D07393A7_B0J5_1_Amp_R_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTGA NCATTTATNN GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA   781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGTGTCG   841 GGGCTGGNTT AACTATGCGG CATCNNANCA GANTGTACTG ANAGTGNNNC ATANATCACG   901 ACATTACTAT ATATATAATA TANGAAGCNT TTAATAGAAC AGCATCGNAA TATATGTGNA   961 CTTTGCANNN ATGANGCCNN ANGNNANNAN NNNNNNANNN TCTTNNNNNN AAAANNANCN 1021 TNNNNNNNNT NNNNNNNNNN //
Primer: Sequence file: RDB18573_B0J5g.seq
1

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

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• S. cerevisiae IFA38 and ortholog

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External Database
S. cerevisiae IFA38

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Aequorea victoria GFP

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• Visualizing multiple inter-organelle contact sites (English text)

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