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pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus.

Catalog number RDB18573
Resource name pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
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RDB18573 pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18573_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
Sequence file: RDB18573_B0J5a.seq check
>D07393A7_B0J5_1_HB-NOS_R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT AANGCCNGGG TTTTCCCNGT CACGACGTTG
   61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
  121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
  181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
  241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
  301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
  361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
  421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
  481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
  541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
  601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
  661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
  721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT
  781 GACTTTTATG CAACAGCTTC NAGANGCTGG GGAAAGANTT ANGTGTATCA ATGGTCTTTT
  841 ATGGGTTGTT TTTGGGCTTG GNGTCTTAAA ATGTACAACG TTATCACTTA NATTTTTTAG
  901 CTCTNATTTT TGATCTTTTT TTTACTACCA GCAGTCAATT TNCGANAAGT ATNGNNGCTA
961 AAACTGGNNA AATACTGNGC TATCACNNGN NGNNNGNGNN NGNNNTNNNN NNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter)
Sequence file: RDB18573_B0J5b.seq check
>D07393A7_B0J5_1_SV40pA3'ElementReverse_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN GNNNNNNGCN NGCANCGCGG CCTTTTTACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
  121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
  181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
  241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
  301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
  361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
  421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
  481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
  541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
  601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT
  661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT
  721 AACTAATTAC ATGACTCGAG GTCGACNGTA TCGATAAGCT TGATATCGAA TTCTTATGTT
  781 ATCCCTGCAG CATTGACGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACNTCCA
  841 CCACTTCNGC CNTTATCGNC ATCATCCTTA TAGNCNATGT CATGATCCTT ATAATCGCCN
901 TCATGGNCTT TGTAATCNCC ACNNGCAGAN GANNNNTCCN TTTTNNNNNN NNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Ifa38 3')
Sequence file: RDB18573_B0J5c.seq check
>D07393A7_B0J5_1_CYC1ter_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NGNNNNAGCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
  121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
  181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
  241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT
  301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA
  361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT
  421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT
  481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT
  541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC
  601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG
  661 GGTGGGAATC AGACCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT
  721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA
  781 TGTAGCAGTG TTATTGATAG TGATAATATT TCTAAGCTCC TTCTCTTCTG TTTCCAAAAA
  841 TGGAACAGGA ATGGAGTGTG ATTGANNAAC ATTATTGANN NAAACGGNGA NTGGTAACTG
  901 CGCACANAAT TCCTNNANGN ANTCATAGTT GGANTCNTTA NNCNCCNNNN NGTCNANNGN
  961 NANAATCTTT ANNNCGANNT GATGNNGNNN NNCNANNTNN TTTTNNNNAN NNCNNNNNNN
1021 TNNNNNNNNN N
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB18573_B0J5d.seq check
>D07393A7_B0J5_1_TRP1pro_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCT NGGNNTNNGT CTGTTATTAA TTTCNCAGGT
   61 AGTTCTGGTC CATTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT
  121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT
  181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC
  241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG
  301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC
  361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA
  421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA
  481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG
  541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT
  601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT
  661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT
  721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG
  781 CACAGATGCG TAANGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTNAT ATTTTGTTAA
  841 AATTCGCGTT NAATTTTTGT TAAATCAGCT CATTTTTTAA CCNATANNNC GAAATCGGCA
  901 AAATCCNNTA TAANTCAAAA GAATAGACCG ANATANGGNT NNANTGNTGN TNNCNNTTTG
  961 GNANCNNGAN NNCNCNANTN NNNNNNNNGN NNTNNNNNNN NNANNGNNNN NANNNNNNNN
1021 NN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB18573_B0J5e.seq check
>D07393A7_B0J5_1_TDH3pro_F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGNNNTTCTG NNNNCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
  121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
  181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
  241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
  301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
  361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
  421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
  481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
  541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
  601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
  661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
  721 CGGGTACTCG TGGTTTGATC TTAACCNTGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
  781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACNAGG TTGGTCTAAC TCTTTGGCTG
  841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTTCATATTT GGNCACTANC
  901 TCGANNNCTA AAATCANAAG ATCATCNTTT NNNGANNCNN AATNCNCNNN ANNTNGTNAA
961 NNNNNNNTNN ANNNNNNNNN N
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18573_B0J5f.seq check
>D07393A7_B0J5_1_Amp_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGA NCATTTATNN GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
  181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
  241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
  301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
  361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
  421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
  481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
  541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
  601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
  781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGTGTCG
  841 GGGCTGGNTT AACTATGCGG CATCNNANCA GANTGTACTG ANAGTGNNNC ATANATCACG
  901 ACATTACTAT ATATATAATA TANGAAGCNT TTAATAGAAC AGCATCGNAA TATATGTGNA
  961 CTTTGCANNN ATGANGCCNN ANGNNANNAN NNNNNNANNN TCTTNNNNNN AAAANNANCN
1021 TNNNNNNNNT NNNNNNNNNN
//
Primer:
Sequence file: RDB18573_B0J5g.seq check
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl