Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus.

Catalog number RDB18573
Resource name pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18573 pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18573_B0J5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
          Sequence file: RDB18573_B0J5a.seq check
          >D07393A7_B0J5_1_HB-NOS_R_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT AANGCCNGGG TTTTCCCNGT CACGACGTTG
             61 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG AGCTCAGTTT
            121 ATCATTATCA ATACTCGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA GTGATTTTCC
            181 TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA
            241 ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC
            301 CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA
            361 ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA
            421 GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT
            481 GATGCAACCT GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC
            541 TCATTTTCTT ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA
            601 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG
            661 GTAGGTATTG ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT
            721 AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT
            781 GACTTTTATG CAACAGCTTC NAGANGCTGG GGAAAGANTT ANGTGTATCA ATGGTCTTTT
            841 ATGGGTTGTT TTTGGGCTTG GNGTCTTAAA ATGTACAACG TTATCACTTA NATTTTTTAG
            901 CTCTNATTTT TGATCTTTTT TTTACTACCA GCAGTCAATT TNCGANAAGT ATNGNNGCTA
          961 AAACTGGNNA AATACTGNGC TATCACNNGN NGNNNGNGNN NGNNNTNNNN NNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter)
          Sequence file: RDB18573_B0J5b.seq check
          >D07393A7_B0J5_1_SV40pA3'ElementReverse_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNAN GNNNNNNGCN NGCANCGCGG CCTTTTTACG GTTCCTGGCC
             61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
            121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
            181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
            241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
            301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
            361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
            421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
            481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
            541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
            601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT
            661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT
            721 AACTAATTAC ATGACTCGAG GTCGACNGTA TCGATAAGCT TGATATCGAA TTCTTATGTT
            781 ATCCCTGCAG CATTGACGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACNTCCA
            841 CCACTTCNGC CNTTATCGNC ATCATCCTTA TAGNCNATGT CATGATCCTT ATAATCGCCN
          901 TCATGGNCTT TGTAATCNCC ACNNGCAGAN GANNNNTCCN TTTTNNNNNN NNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Ifa38 3')
          Sequence file: RDB18573_B0J5c.seq check
          >D07393A7_B0J5_1_CYC1ter_R_H02_23_ABI24.ab1
              1 NNNNNNNNNN NGNNNNAGCG GNNGTGGGGG GNNGGCGTGA ATGTAAGCGT GACATAACTA
             61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
            121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
            181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
            241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTCCTTTTTA ACCTGTCTTG CGGCTTTTTT
            301 TAAGGCTCTA ATTCTGATAG ATTTATGGAA GGAATAATTA ATGGAGTTAA CAATCTTAGA
            361 GTAAACGCCA AAGGTCTCTG TAATTACAAA TTGATACACT GCGTGCGCCC AGTAAGGGGT
            421 CATAGTAGCG TATCTTTCTT GAGAGCCGCA GCGTCTGCCA ACGCTTCTTA AAGTGGATTT
            481 TACAAACTGT TGTGGATTTG GGATCATTAA AGATGATCTT CTGATTTTAG ACATCGAGCT
            541 AGTGACCAAA TATGAAATGA TTAATTCAAC ATCGATAGCA TCTTTAGATA ATTCCCCAGC
            601 CAAAGAGTTA GACCAACCTT GTAAGAATGA TTTCGAACCA CTGTATGTAG CCAAAAGTGG
            661 GGTGGGAATC AGACCACCAA ATGAGCCCAT GGTTAAGATC AAACCACGAG TACCCGACTT
            721 CTTGTTTTCA GCTTTCACAG TTTCCACAAT CTTTGGTGCA ATGATTTGTG TAATTAATAA
            781 TGTAGCAGTG TTATTGATAG TGATAATATT TCTAAGCTCC TTCTCTTCTG TTTCCAAAAA
            841 TGGAACAGGA ATGGAGTGTG ATTGANNAAC ATTATTGANN NAAACGGNGA NTGGTAACTG
            901 CGCACANAAT TCCTNNANGN ANTCATAGTT GGANTCNTTA NNCNCCNNNN NGTCNANNGN
            961 NANAATCTTT ANNNCGANNT GATGNNGNNN NNCNANNTNN TTTTNNNNAN NNCNNNNNNN
          1021 TNNNNNNNNN N
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB18573_B0J5d.seq check
          >D07393A7_B0J5_1_TRP1pro_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCT NGGNNTNNGT CTGTTATTAA TTTCNCAGGT
             61 AGTTCTGGTC CATTGGTGAA AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCT
            121 CTAGATTCCG ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT
            181 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA TAGTTCAGGC
            241 ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA AGGAGGATGT TTTGGCTCTG
            301 GTCAATGATT ACGGCATTGA TATCGTCCAA CTGCATGGAG ATGAGTCGTG GCAAGAATAC
            361 CAAGAGTTCC TCGGTTTGCC AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA
            421 CTACTCAGTG CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA
            481 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG AAGGCAAGAG
            541 AGCCCCGAAA GCTTACATTT TATGTTAGCT GGTGGACTGA CGCCAGAAAA TGTTGGTGAT
            601 GCGCTTAGAT TAAATGGCGT TATTGGTGTT GATGTAAGCG GAGGTGTGGA GACAAATGGT
            661 GTAAAAGACT CTAACAAAAT AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT
            721 GAGTAGTATT TATTTAAGTA TTGTTTGTGC ACTTGCCGAT CTATGCGGTG TGAAATACCG
            781 CACAGATGCG TAANGAGAAA ATACCGCATC ANGAAATTGT AAGCGTTNAT ATTTTGTTAA
            841 AATTCGCGTT NAATTTTTGT TAAATCAGCT CATTTTTTAA CCNATANNNC GAAATCGGCA
            901 AAATCCNNTA TAANTCAAAA GAATAGACCG ANATANGGNT NNANTGNTGN TNNCNNTTTG
            961 GNANCNNGAN NNCNCNANTN NNNNNNNNGN NNTNNNNNNN NNANNGNNNN NANNNNNNNN
          1021 NN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB18573_B0J5e.seq check
          >D07393A7_B0J5_1_TDH3pro_F_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN TGNNNTTCTG NNNNCTATTT CTTAAACTTC TTAAATTCTA
             61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
            121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
            181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
            241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
            301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
            361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
            421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
            481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
            541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
            601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
            661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
            721 CGGGTACTCG TGGTTTGATC TTAACCNTGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
            781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACNAGG TTGGTCTAAC TCTTTGGCTG
            841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTTCATATTT GGNCACTANC
            901 TCGANNNCTA AAATCANAAG ATCATCNTTT NNNGANNCNN AATNCNCNNN ANNTNGTNAA
          961 NNNNNNNTNN ANNNNNNNNN N
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB18573_B0J5f.seq check
          >D07393A7_B0J5_1_Amp_R_H03_24_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTGA NCATTTATNN GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
            781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGTGTCG
            841 GGGCTGGNTT AACTATGCGG CATCNNANCA GANTGTACTG ANAGTGNNNC ATANATCACG
            901 ACATTACTAT ATATATAATA TANGAAGCNT TTAATAGAAC AGCATCGNAA TATATGTGNA
            961 CTTTGCANNN ATGANGCCNN ANGNNANNAN NNNNNNANNN TCTTNNNNNN AAAANNANCN
          1021 TNNNNNNNNT NNNNNNNNNN
          //
          Primer:
          Sequence file: RDB18573_B0J5g.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl