RDB18572 - pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11

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pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 (#RDB18572)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: mCherry-GFP11; location: endoplasmic reticulum.

Alternative name	pSFL61, pRS316-GPDp-Ifa38-mCherry-GFP11
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment	Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone	pRS316 (plasmid)
Selectable markers	Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name	S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA Discosoma sp RFP cDNA
Depositor\|Developer	Tamura, Yasushi \|

Sequence (full)	RDB18572zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18572	pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18572).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18572_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18572_B0J5a.seq
>D07393A6_B0J5_2-HB-NOS_R_A04_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGGGTNNN GCCNGGGTTT TCCCNGTCNC GACGTTGTAA 61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC 121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA 181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA 241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG 301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC 361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG 421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT 481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA 541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG 601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA 661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT 721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCTCTAG 781 AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCNAGAN GCTGGGGNNA GANTTAGGTG 841 TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA CAACGNTATC 901 ACTTANATTT TTANCTCTTA TTTTTTGATC TTTTTTTTAC TANNANCANN CNANTTNCNA 961 NNNNNATNGN NGNNNAAACN NGNNAAATAC NGNGCNATCN NNNGNNGNNN NNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB18572_B0J5b.seq
>D07393A6_B0J5_2_SV40pA3'ElementReverse_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNAANN GCNNGCAACG CGGCCTTTTT ACGGTTCCTG 61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 421 AGCTATGACC ATGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG 481 GTACCGGCCG CAAATTAAAG CCTTCGAGCG TCCCAAAACC TTCTCAAGCA AGGTTTTCAG 541 TATAATGTTA CATGCGTACA CGCGTCTGTA CAGAAAAAAA AGAAAAATTT GAAATATAAA 601 TAACGTTCTT AATACTAACA TAACTATAAA AAAATAAATA GGGACCTAGA CTTCAGGTTG 661 TCTAACTCCT TCCTTTTCGG TTAGAGCGGA TGTGGGGGGA GGGCGTGAAT GTAAGCGTGA 721 CATAACTAAT TACATGACTC GAGGTCGANT CAAGTTATGC CGGCAGCATT GACGTACTCG 781 TGCAACACCA TGTGATCCCT GCTTGTACTC CCTCCNCCTG ANCCACCAGA TCTCTNGTAC 841 AGTTCGTCCA TTNCACCCNN ANANNGTCTG NNNNCNGCTC NTTCATACNG NNNCNNNANN 901 GNGNAANCTN CNNNGNGGNN ANNNANNTNN NGCTNGATGT NCACATTATA CNNNNCNNNN 961 NNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP11, mCherry) Sequence file: RDB18572_B0J5c.seq
>D07393A6_B0J5_2-CYC1Ter_R_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGNNGNGGGG GGNNGGCGTG NATGTAAGCG TGACATAACT 61 AATTACATGA CTCGAGGTCG ACTCAAGTTA TGCCGGCAGC ATTGACGTAC TCGTGCAACA 121 CCATGTGATC CCTGCTTGTA CTCCCTCCGC CTGACCCACC AGATCTCTTG TACAGTTCGT 181 CCATTCCACC CGTAGAATGT CTGCCTTCGG CTCGTTCATA CTGTTCCACA ATGGTGTAAT 241 CTTCATTGTG GCTAGTGATA TCCAGCTTGA TGTTCACATT ATACGCGCCT GGAAGCTGAA 301 CGGGTTTCTT TGCCTTGTAG GTGGTCTTGA CTTCGGCATC GTAATGGCCT CCATCCTTCA 361 GTTTCAGGCG CTGTTTGATT TCGCCCTTGA GTGCACCATC TTCGGGATAC ATACGCTCTG 421 AGCTGGCTTC CCATCCCATC GTTTTCTTCT GCATCACTGG CCCGTCGGAA GGAAAGTTGG 481 TTCCTCTCAG CTTCACCTTG TAGATGAACT CACCGTCTTG GAGGGAACTG TCCTGAGTCA 541 CTGTGACAAC TCCTCCGTCC TCGAAGTTCA TGACCCTCTC CCATTTGAAC CCCTCGGGAA 601 AGGAGAGTTT CAGGTAGTCT GGAATGTCCG CAGGATGTTT CACGTATGCC TTGCTCCCAT 661 ACATGAACTG GGGAGACAGA ATGTCCCAGG CAAACGGCAG AGGTCCTCCT TTTGTCACCT 721 TGAGTTTAGC GGTTTGTGTC CCCTCATATG GNCGGCCTTC CCCTTCACCC TCAATCTCNA 781 ACTCGTGTCC ATTGACACTG CCCTCCATGT GTACCTTGAA CCGCATAAAC TCCTTTATGA 841 NGGCCATGTT ATCCTCCTCA CCCNTGCNAA CGCCGCCNGC GGAAAGCTTT TCCTTTTTNA 901 NCTGTCNNGC GGCTTTTTTT TANNGNNNTA ANTCTGATAN ANTTATGGAA NGAANANTTN 961 NNGGNANTTA ANNNTCNNNN ANTAAANNNC NNAANNNNNN NNNN //
Primer: URA3pro_F (Pr0170) Region: URA3 pro, URA3 Sequence file: RDB18572_B0J5d.seq
>D07393A6_B0J5_2_URA3pro_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNANNN NNNANNTGCC NNGTATTCTT AACCCAACTG CACAGAACAA 61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA 121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT 181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG 241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG 301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA 361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA 421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG 481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG 541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG 601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG 661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG 721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ANGTGGTCTC TACAGGNTCT GACATTATTA 781 TTGTTGGAAG AGGANTATTT GCAAAGGGAA NGGANGCTAN NGTAGAGGGT GAACGTTACA 841 GAAAAGCANG CTGGGAANCA TATTNGANAA NANGCGGCCN GCAAAACTNA AAAAACTGNA 901 TTATNAGTAA ATGCNNGTAN ACTAAACTCN CAANNTANAG CTTNNNTTTN ANTATATCAG 961 TTANNACCCT NNGCNGNNGN GAANNNCNNN NNNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18572_B0J5e.seq
>D07393A6_B0J5_2_TDH3pro_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNGNNTG NNNTCTGTNA NTCTATTTCT TAAACTTCTT AAATTCTACT 61 TTTATAGTTA GTCTTTTTTT TAGTTTTAAA ACACCAGAAC TTAGTTTCGA CGGATGCGGC 121 CGCTCTAGAA CTAGTGGATC CATGACTTTT ATGCAACAGC TTCAAGAGGC TGGGGAAAGA 181 TTTAGGTGTA TCAATGGTCT TTTATGGGTT GTTTTTGGGC TTGGTGTCTT AAAATGTACA 241 ACGTTATCAC TTAGATTTTT AGCTCTTATT TTTGATCTTT TTTTACTACC AGCAGTCAAT 301 TTCGACAAGT ATGGTGCTAA AACTGGTAAA TACTGTGCTA TCACTGGTGC AAGTGACGGT 361 ATTGGTAAAG AATTTGCTAG ACAAATGGCG AAACGTGGCT TCAACTTGGT ATTGATCTCG 421 AGAACGCAAT CTAAATTGGA GGCTTTACAA AAAGAACTAG AAGATCAACA TCATGTCGTT 481 GTAAAGATTC TAGCCATTGA CATTGCGGAG GATAAAGAAT CCAACTATGA ATCCATTAAG 541 GAATTGTGTG CGCAGTTACC AATCACCGTT TTGGTCAATA ATGTTGGTCA ATCACACTCC 601 ATTCCTGTTC CATTTTTGGA AACAGAAGAG AAGGAGCTTA GAAATATTAT CACTATCAAT 661 AACACTGCTA CATTATTAAT TACACAAATC ATTGCACCAA AGATTGTGGA AACTGTGAAA 721 GCTGANAACA AGAAGTCGGG TACTCGTGGT TTGATCTTAA CCATGGGCTC ATTTGGTGGT 781 CTGATTCCCA CCCCACTTTT GGCTACATAC AGNGGTTCGA AATCATTCTT ACAAGGTTGG 841 TCTAACTCTN NGGCTGGGGA ANTATCTAAA GATGCTATCN ATGTNGAATT ANTCATTTCA 901 TATTTGGNCA CTANCTCNAN GTCTAAAANC ANAANNNCAT CNTNNANGAT CCNNAANNNN 961 NNNNNN //
Primer: Ifa38-C_F (Pr0897) Region: insert mid (Ifa38 3', mCherry 5') Sequence file: RDB18572_B0J5f.seq
>D07393A6_B0J5_2_Ifa38_C-F_B10_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNNGNNNN CTTTGGCGTT NANTCTAAGA NNTGTNAACT 61 CCNTTAATTA TTCCTTCCNT AAATCTATCA GAATTAGAGC CTTAAAAAAA GCCGCAAGAC 121 AGGTTAAAAA GGAAAAGCTT TCCGCTGGCG GCGTTAGCAA GGGTGAGGAG GATAACATGG 181 CCATCATAAA GGAGTTTATG CGGTTCAAGG TACACATGGA GGGCAGTGTC AATGGACACG 241 AGTTTGAGAT TGAGGGTGAA GGGGAAGGCC GGCCATATGA GGGGACACAA ACCGCTAAAC 301 TCAAGGTGAC AAAAGGAGGA CCTCTGCCGT TTGCCTGGGA CATTCTGTCT CCCCAGTTCA 361 TGTATGGGAG CAAGGCATAC GTGAAACATC CTGCGGACAT TCCAGACTAC CTGAAACTCT 421 CCTTTCCCGA GGGGTTCAAA TGGGAGAGGG TCATGAACTT CGAGGACGGA GGAGTTGTCA 481 CAGTGACTCA GGACAGTTCC CTCCAAGACG GTGAGTTCAT CTACAAGGTG AAGCTGAGAG 541 GAACCAACTT TCCTTCCGAC GGGCCAGTGA TGCAGAAGAA AACGATGGGA TGGGAAGCCA 601 GCTCAGAGCG TATGTATCCC GAAGATGGTG CACTCAAGGG CGAAATCAAA CAGCGCCTGA 661 AACTGAAGGA TGGAGGCCAT TACGATGCCG AAGTCAAGAC CACCTACAAG GCAAAGAAAC 721 CCGTTCAGCT TCCAGGCGCG TATAATGTGA ACATCAAGCT GGATATCACT AGCCACAATG 781 AAGATTACAC CATTGTGGAN CAGTATGAAC GANCCGAANG CAGACATTCT ACGGGTGGAA 841 TGGACGAACT GTACAAGAGA TCTGGTGGGT CANGCGGAGG GAGTACAAGC AGGNNTCACA 901 TGGNGTTGCA CGANTACGTC NNNGCTGCCN NCATAACNNN ANTCGANCNN NNNNCATGTA 961 ATNANNTANG NNNCNNNNAC NTTNNNNNCN NNCCCCNNNN NNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18572_B0J5g.seq
>D07393A6_B0J5_2_Amp_R_H01_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNA NCANTTATNN GGGTTATTGT CTCNTGAGCG GANACATATT 61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA 181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT 241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT 301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG 361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC 421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT 481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT 541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT 601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT 661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG 721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA 781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA NGGCGCGNCA GCGGGTGTTG GCGGGNGTCG 841 GGGCTGGCTT ANCTATGCGG CATCAGANCA NANTGTACTG ANAGTGNACC ATANCACCTT 901 TTCNNTTCAT CATTTTTTTT TTANNCTTTT TTTTGANTNN NNNTNNNNTN NAAATTTTTT 961 TGNNTCNGNN NNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]