Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: mCherry-GFP11; location: endoplasmic reticulum.

Sequence (full)	RDB18572zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18572_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18572_B0J5a.seq
>D07393A6_B0J5_2-HB-NOS_R_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGGGTNNN GCCNGGGTTT TCCCNGTCNC GACGTTGTAA    61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC   121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA   181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA   241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG   301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC   361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG   421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT   481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA   541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG   601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA   661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT   721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCTCTAG   781 AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCNAGAN GCTGGGGNNA GANTTAGGTG   841 TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA CAACGNTATC   901 ACTTANATTT TTANCTCTTA TTTTTTGATC TTTTTTTTAC TANNANCANN CNANTTNCNA 961 NNNNNATNGN NGNNNAAACN NGNNAAATAC NGNGCNATCN NNNGNNGNNN NNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter) Sequence file: RDB18572_B0J5b.seq
>D07393A6_B0J5_2_SV40pA3'ElementReverse_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNAANN GCNNGCAACG CGGCCTTTTT ACGGTTCCTG    61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT   121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC   181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG   241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT   301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT   361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC   421 AGCTATGACC ATGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG   481 GTACCGGCCG CAAATTAAAG CCTTCGAGCG TCCCAAAACC TTCTCAAGCA AGGTTTTCAG   541 TATAATGTTA CATGCGTACA CGCGTCTGTA CAGAAAAAAA AGAAAAATTT GAAATATAAA   601 TAACGTTCTT AATACTAACA TAACTATAAA AAAATAAATA GGGACCTAGA CTTCAGGTTG   661 TCTAACTCCT TCCTTTTCGG TTAGAGCGGA TGTGGGGGGA GGGCGTGAAT GTAAGCGTGA   721 CATAACTAAT TACATGACTC GAGGTCGANT CAAGTTATGC CGGCAGCATT GACGTACTCG   781 TGCAACACCA TGTGATCCCT GCTTGTACTC CCTCCNCCTG ANCCACCAGA TCTCTNGTAC   841 AGTTCGTCCA TTNCACCCNN ANANNGTCTG NNNNCNGCTC NTTCATACNG NNNCNNNANN   901 GNGNAANCTN CNNNGNGGNN ANNNANNTNN NGCTNGATGT NCACATTATA CNNNNCNNNN 961 NNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP11, mCherry) Sequence file: RDB18572_B0J5c.seq
>D07393A6_B0J5_2-CYC1Ter_R_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNGNGGGG GGNNGGCGTG NATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACTCAAGTTA TGCCGGCAGC ATTGACGTAC TCGTGCAACA   121 CCATGTGATC CCTGCTTGTA CTCCCTCCGC CTGACCCACC AGATCTCTTG TACAGTTCGT   181 CCATTCCACC CGTAGAATGT CTGCCTTCGG CTCGTTCATA CTGTTCCACA ATGGTGTAAT   241 CTTCATTGTG GCTAGTGATA TCCAGCTTGA TGTTCACATT ATACGCGCCT GGAAGCTGAA   301 CGGGTTTCTT TGCCTTGTAG GTGGTCTTGA CTTCGGCATC GTAATGGCCT CCATCCTTCA   361 GTTTCAGGCG CTGTTTGATT TCGCCCTTGA GTGCACCATC TTCGGGATAC ATACGCTCTG   421 AGCTGGCTTC CCATCCCATC GTTTTCTTCT GCATCACTGG CCCGTCGGAA GGAAAGTTGG   481 TTCCTCTCAG CTTCACCTTG TAGATGAACT CACCGTCTTG GAGGGAACTG TCCTGAGTCA   541 CTGTGACAAC TCCTCCGTCC TCGAAGTTCA TGACCCTCTC CCATTTGAAC CCCTCGGGAA   601 AGGAGAGTTT CAGGTAGTCT GGAATGTCCG CAGGATGTTT CACGTATGCC TTGCTCCCAT   661 ACATGAACTG GGGAGACAGA ATGTCCCAGG CAAACGGCAG AGGTCCTCCT TTTGTCACCT   721 TGAGTTTAGC GGTTTGTGTC CCCTCATATG GNCGGCCTTC CCCTTCACCC TCAATCTCNA   781 ACTCGTGTCC ATTGACACTG CCCTCCATGT GTACCTTGAA CCGCATAAAC TCCTTTATGA   841 NGGCCATGTT ATCCTCCTCA CCCNTGCNAA CGCCGCCNGC GGAAAGCTTT TCCTTTTTNA   901 NCTGTCNNGC GGCTTTTTTT TANNGNNNTA ANTCTGATAN ANTTATGGAA NGAANANTTN 961 NNGGNANTTA ANNNTCNNNN ANTAAANNNC NNAANNNNNN NNNN //
Primer: URA3pro_F (Pr0170) Region: URA3 pro, URA3 Sequence file: RDB18572_B0J5d.seq
>D07393A6_B0J5_2_URA3pro_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNANNN NNNANNTGCC NNGTATTCTT AACCCAACTG CACAGAACAA    61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA   121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT   181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG   241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG   301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA   361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA   421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG   481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG   541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG   601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG   661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG   721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ANGTGGTCTC TACAGGNTCT GACATTATTA   781 TTGTTGGAAG AGGANTATTT GCAAAGGGAA NGGANGCTAN NGTAGAGGGT GAACGTTACA   841 GAAAAGCANG CTGGGAANCA TATTNGANAA NANGCGGCCN GCAAAACTNA AAAAACTGNA   901 TTATNAGTAA ATGCNNGTAN ACTAAACTCN CAANNTANAG CTTNNNTTTN ANTATATCAG 961 TTANNACCCT NNGCNGNNGN GAANNNCNNN NNNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18572_B0J5e.seq
>D07393A6_B0J5_2_TDH3pro_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNGNNTG NNNTCTGTNA NTCTATTTCT TAAACTTCTT AAATTCTACT    61 TTTATAGTTA GTCTTTTTTT TAGTTTTAAA ACACCAGAAC TTAGTTTCGA CGGATGCGGC   121 CGCTCTAGAA CTAGTGGATC CATGACTTTT ATGCAACAGC TTCAAGAGGC TGGGGAAAGA   181 TTTAGGTGTA TCAATGGTCT TTTATGGGTT GTTTTTGGGC TTGGTGTCTT AAAATGTACA   241 ACGTTATCAC TTAGATTTTT AGCTCTTATT TTTGATCTTT TTTTACTACC AGCAGTCAAT   301 TTCGACAAGT ATGGTGCTAA AACTGGTAAA TACTGTGCTA TCACTGGTGC AAGTGACGGT   361 ATTGGTAAAG AATTTGCTAG ACAAATGGCG AAACGTGGCT TCAACTTGGT ATTGATCTCG   421 AGAACGCAAT CTAAATTGGA GGCTTTACAA AAAGAACTAG AAGATCAACA TCATGTCGTT   481 GTAAAGATTC TAGCCATTGA CATTGCGGAG GATAAAGAAT CCAACTATGA ATCCATTAAG   541 GAATTGTGTG CGCAGTTACC AATCACCGTT TTGGTCAATA ATGTTGGTCA ATCACACTCC   601 ATTCCTGTTC CATTTTTGGA AACAGAAGAG AAGGAGCTTA GAAATATTAT CACTATCAAT   661 AACACTGCTA CATTATTAAT TACACAAATC ATTGCACCAA AGATTGTGGA AACTGTGAAA   721 GCTGANAACA AGAAGTCGGG TACTCGTGGT TTGATCTTAA CCATGGGCTC ATTTGGTGGT   781 CTGATTCCCA CCCCACTTTT GGCTACATAC AGNGGTTCGA AATCATTCTT ACAAGGTTGG   841 TCTAACTCTN NGGCTGGGGA ANTATCTAAA GATGCTATCN ATGTNGAATT ANTCATTTCA   901 TATTTGGNCA CTANCTCNAN GTCTAAAANC ANAANNNCAT CNTNNANGAT CCNNAANNNN 961 NNNNNN //
Primer: Ifa38-C_F (Pr0897) Region: insert mid (Ifa38 3', mCherry 5') Sequence file: RDB18572_B0J5f.seq
>D07393A6_B0J5_2_Ifa38_C-F_B10_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNNNGNNNN CTTTGGCGTT NANTCTAAGA NNTGTNAACT    61 CCNTTAATTA TTCCTTCCNT AAATCTATCA GAATTAGAGC CTTAAAAAAA GCCGCAAGAC   121 AGGTTAAAAA GGAAAAGCTT TCCGCTGGCG GCGTTAGCAA GGGTGAGGAG GATAACATGG   181 CCATCATAAA GGAGTTTATG CGGTTCAAGG TACACATGGA GGGCAGTGTC AATGGACACG   241 AGTTTGAGAT TGAGGGTGAA GGGGAAGGCC GGCCATATGA GGGGACACAA ACCGCTAAAC   301 TCAAGGTGAC AAAAGGAGGA CCTCTGCCGT TTGCCTGGGA CATTCTGTCT CCCCAGTTCA   361 TGTATGGGAG CAAGGCATAC GTGAAACATC CTGCGGACAT TCCAGACTAC CTGAAACTCT   421 CCTTTCCCGA GGGGTTCAAA TGGGAGAGGG TCATGAACTT CGAGGACGGA GGAGTTGTCA   481 CAGTGACTCA GGACAGTTCC CTCCAAGACG GTGAGTTCAT CTACAAGGTG AAGCTGAGAG   541 GAACCAACTT TCCTTCCGAC GGGCCAGTGA TGCAGAAGAA AACGATGGGA TGGGAAGCCA   601 GCTCAGAGCG TATGTATCCC GAAGATGGTG CACTCAAGGG CGAAATCAAA CAGCGCCTGA   661 AACTGAAGGA TGGAGGCCAT TACGATGCCG AAGTCAAGAC CACCTACAAG GCAAAGAAAC   721 CCGTTCAGCT TCCAGGCGCG TATAATGTGA ACATCAAGCT GGATATCACT AGCCACAATG   781 AAGATTACAC CATTGTGGAN CAGTATGAAC GANCCGAANG CAGACATTCT ACGGGTGGAA   841 TGGACGAACT GTACAAGAGA TCTGGTGGGT CANGCGGAGG GAGTACAAGC AGGNNTCACA   901 TGGNGTTGCA CGANTACGTC NNNGCTGCCN NCATAACNNN ANTCGANCNN NNNNCATGTA 961 ATNANNTANG NNNCNNNNAC NTTNNNNNCN NNCCCCNNNN NNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18572_B0J5g.seq
>D07393A6_B0J5_2_Amp_R_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNA NCANTTATNN GGGTTATTGT CTCNTGAGCG GANACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA   181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT   241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT   301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG   361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC   421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT   481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT   541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT   601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA   781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA NGGCGCGNCA GCGGGTGTTG GCGGGNGTCG   841 GGGCTGGCTT ANCTATGCGG CATCAGANCA NANTGTACTG ANAGTGNACC ATANCACCTT   901 TTCNNTTCAT CATTTTTTTT TTANNCTTTT TTTTGANTNN NNNTNNNNTN NAAATTTTTT 961 TGNNTCNGNN NNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.13

GNP_filter3_RDBDEP_html_231213.pl