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pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus.

Catalog number RDB18572
Resource name pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with mCherry-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA Discosoma sp RFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
S. cerevisiae IFA38 Aequorea victoria GFP
    Discosoma sp RFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18572 pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18572zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18572_B0J5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
          Sequence file: RDB18572_B0J5a.seq check
          >D07393A6_B0J5_2-HB-NOS_R_A04_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGGGTNNN GCCNGGGTTT TCCCNGTCNC GACGTTGTAA
             61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
            121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
            181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
            241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
            301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
            361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
            421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
            481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
            541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
            601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA
            661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT
            721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCTCTAG
            781 AACTAGTGGA TCCATGACTT TTATGCAACA GCTTCNAGAN GCTGGGGNNA GANTTAGGTG
            841 TATCAATGGT CTTTTATGGG TTGTTTTTGG GCTTGGTGTC TTAAAATGTA CAACGNTATC
            901 ACTTANATTT TTANCTCTTA TTTTTTGATC TTTTTTTTAC TANNANCANN CNANTTNCNA
          961 NNNNNATNGN NGNNNAAACN NGNNAAATAC NGNGCNATCN NNNGNNGNNN NNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter)
          Sequence file: RDB18572_B0J5b.seq check
          >D07393A6_B0J5_2_SV40pA3'ElementReverse_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNAANN GCNNGCAACG CGGCCTTTTT ACGGTTCCTG
             61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
            121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
            181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
            241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
            301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT
            361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC
            421 AGCTATGACC ATGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
            481 GTACCGGCCG CAAATTAAAG CCTTCGAGCG TCCCAAAACC TTCTCAAGCA AGGTTTTCAG
            541 TATAATGTTA CATGCGTACA CGCGTCTGTA CAGAAAAAAA AGAAAAATTT GAAATATAAA
            601 TAACGTTCTT AATACTAACA TAACTATAAA AAAATAAATA GGGACCTAGA CTTCAGGTTG
            661 TCTAACTCCT TCCTTTTCGG TTAGAGCGGA TGTGGGGGGA GGGCGTGAAT GTAAGCGTGA
            721 CATAACTAAT TACATGACTC GAGGTCGANT CAAGTTATGC CGGCAGCATT GACGTACTCG
            781 TGCAACACCA TGTGATCCCT GCTTGTACTC CCTCCNCCTG ANCCACCAGA TCTCTNGTAC
            841 AGTTCGTCCA TTNCACCCNN ANANNGTCTG NNNNCNGCTC NTTCATACNG NNNCNNNANN
            901 GNGNAANCTN CNNNGNGGNN ANNNANNTNN NGCTNGATGT NCACATTATA CNNNNCNNNN
          961 NNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP11, mCherry)
          Sequence file: RDB18572_B0J5c.seq check
          >D07393A6_B0J5_2-CYC1Ter_R_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGNNGNGGGG GGNNGGCGTG NATGTAAGCG TGACATAACT
             61 AATTACATGA CTCGAGGTCG ACTCAAGTTA TGCCGGCAGC ATTGACGTAC TCGTGCAACA
            121 CCATGTGATC CCTGCTTGTA CTCCCTCCGC CTGACCCACC AGATCTCTTG TACAGTTCGT
            181 CCATTCCACC CGTAGAATGT CTGCCTTCGG CTCGTTCATA CTGTTCCACA ATGGTGTAAT
            241 CTTCATTGTG GCTAGTGATA TCCAGCTTGA TGTTCACATT ATACGCGCCT GGAAGCTGAA
            301 CGGGTTTCTT TGCCTTGTAG GTGGTCTTGA CTTCGGCATC GTAATGGCCT CCATCCTTCA
            361 GTTTCAGGCG CTGTTTGATT TCGCCCTTGA GTGCACCATC TTCGGGATAC ATACGCTCTG
            421 AGCTGGCTTC CCATCCCATC GTTTTCTTCT GCATCACTGG CCCGTCGGAA GGAAAGTTGG
            481 TTCCTCTCAG CTTCACCTTG TAGATGAACT CACCGTCTTG GAGGGAACTG TCCTGAGTCA
            541 CTGTGACAAC TCCTCCGTCC TCGAAGTTCA TGACCCTCTC CCATTTGAAC CCCTCGGGAA
            601 AGGAGAGTTT CAGGTAGTCT GGAATGTCCG CAGGATGTTT CACGTATGCC TTGCTCCCAT
            661 ACATGAACTG GGGAGACAGA ATGTCCCAGG CAAACGGCAG AGGTCCTCCT TTTGTCACCT
            721 TGAGTTTAGC GGTTTGTGTC CCCTCATATG GNCGGCCTTC CCCTTCACCC TCAATCTCNA
            781 ACTCGTGTCC ATTGACACTG CCCTCCATGT GTACCTTGAA CCGCATAAAC TCCTTTATGA
            841 NGGCCATGTT ATCCTCCTCA CCCNTGCNAA CGCCGCCNGC GGAAAGCTTT TCCTTTTTNA
            901 NCTGTCNNGC GGCTTTTTTT TANNGNNNTA ANTCTGATAN ANTTATGGAA NGAANANTTN
          961 NNGGNANTTA ANNNTCNNNN ANTAAANNNC NNAANNNNNN NNNN
          //
          Primer: URA3pro_F
          Region: URA3 pro, URA3
          Sequence file: RDB18572_B0J5d.seq check
          >D07393A6_B0J5_2_URA3pro_F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNANNN NNNANNTGCC NNGTATTCTT AACCCAACTG CACAGAACAA
             61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA
            121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT
            181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG
            241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG
            301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA
            361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA
            421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG
            481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG
            541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG
            601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG
            661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG
            721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ANGTGGTCTC TACAGGNTCT GACATTATTA
            781 TTGTTGGAAG AGGANTATTT GCAAAGGGAA NGGANGCTAN NGTAGAGGGT GAACGTTACA
            841 GAAAAGCANG CTGGGAANCA TATTNGANAA NANGCGGCCN GCAAAACTNA AAAAACTGNA
            901 TTATNAGTAA ATGCNNGTAN ACTAAACTCN CAANNTANAG CTTNNNTTTN ANTATATCAG
          961 TTANNACCCT NNGCNGNNGN GAANNNCNNN NNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB18572_B0J5e.seq check
          >D07393A6_B0J5_2_TDH3pro_F_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNGNNTG NNNTCTGTNA NTCTATTTCT TAAACTTCTT AAATTCTACT
             61 TTTATAGTTA GTCTTTTTTT TAGTTTTAAA ACACCAGAAC TTAGTTTCGA CGGATGCGGC
            121 CGCTCTAGAA CTAGTGGATC CATGACTTTT ATGCAACAGC TTCAAGAGGC TGGGGAAAGA
            181 TTTAGGTGTA TCAATGGTCT TTTATGGGTT GTTTTTGGGC TTGGTGTCTT AAAATGTACA
            241 ACGTTATCAC TTAGATTTTT AGCTCTTATT TTTGATCTTT TTTTACTACC AGCAGTCAAT
            301 TTCGACAAGT ATGGTGCTAA AACTGGTAAA TACTGTGCTA TCACTGGTGC AAGTGACGGT
            361 ATTGGTAAAG AATTTGCTAG ACAAATGGCG AAACGTGGCT TCAACTTGGT ATTGATCTCG
            421 AGAACGCAAT CTAAATTGGA GGCTTTACAA AAAGAACTAG AAGATCAACA TCATGTCGTT
            481 GTAAAGATTC TAGCCATTGA CATTGCGGAG GATAAAGAAT CCAACTATGA ATCCATTAAG
            541 GAATTGTGTG CGCAGTTACC AATCACCGTT TTGGTCAATA ATGTTGGTCA ATCACACTCC
            601 ATTCCTGTTC CATTTTTGGA AACAGAAGAG AAGGAGCTTA GAAATATTAT CACTATCAAT
            661 AACACTGCTA CATTATTAAT TACACAAATC ATTGCACCAA AGATTGTGGA AACTGTGAAA
            721 GCTGANAACA AGAAGTCGGG TACTCGTGGT TTGATCTTAA CCATGGGCTC ATTTGGTGGT
            781 CTGATTCCCA CCCCACTTTT GGCTACATAC AGNGGTTCGA AATCATTCTT ACAAGGTTGG
            841 TCTAACTCTN NGGCTGGGGA ANTATCTAAA GATGCTATCN ATGTNGAATT ANTCATTTCA
            901 TATTTGGNCA CTANCTCNAN GTCTAAAANC ANAANNNCAT CNTNNANGAT CCNNAANNNN
          961 NNNNNN
          //
          Primer: Ifa38-C_F
          Region: insert mid (Ifa38 3', mCherry 5')
          Sequence file: RDB18572_B0J5f.seq check
          >D07393A6_B0J5_2_Ifa38_C-F_B10_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTNNNGNNNN CTTTGGCGTT NANTCTAAGA NNTGTNAACT
             61 CCNTTAATTA TTCCTTCCNT AAATCTATCA GAATTAGAGC CTTAAAAAAA GCCGCAAGAC
            121 AGGTTAAAAA GGAAAAGCTT TCCGCTGGCG GCGTTAGCAA GGGTGAGGAG GATAACATGG
            181 CCATCATAAA GGAGTTTATG CGGTTCAAGG TACACATGGA GGGCAGTGTC AATGGACACG
            241 AGTTTGAGAT TGAGGGTGAA GGGGAAGGCC GGCCATATGA GGGGACACAA ACCGCTAAAC
            301 TCAAGGTGAC AAAAGGAGGA CCTCTGCCGT TTGCCTGGGA CATTCTGTCT CCCCAGTTCA
            361 TGTATGGGAG CAAGGCATAC GTGAAACATC CTGCGGACAT TCCAGACTAC CTGAAACTCT
            421 CCTTTCCCGA GGGGTTCAAA TGGGAGAGGG TCATGAACTT CGAGGACGGA GGAGTTGTCA
            481 CAGTGACTCA GGACAGTTCC CTCCAAGACG GTGAGTTCAT CTACAAGGTG AAGCTGAGAG
            541 GAACCAACTT TCCTTCCGAC GGGCCAGTGA TGCAGAAGAA AACGATGGGA TGGGAAGCCA
            601 GCTCAGAGCG TATGTATCCC GAAGATGGTG CACTCAAGGG CGAAATCAAA CAGCGCCTGA
            661 AACTGAAGGA TGGAGGCCAT TACGATGCCG AAGTCAAGAC CACCTACAAG GCAAAGAAAC
            721 CCGTTCAGCT TCCAGGCGCG TATAATGTGA ACATCAAGCT GGATATCACT AGCCACAATG
            781 AAGATTACAC CATTGTGGAN CAGTATGAAC GANCCGAANG CAGACATTCT ACGGGTGGAA
            841 TGGACGAACT GTACAAGAGA TCTGGTGGGT CANGCGGAGG GAGTACAAGC AGGNNTCACA
            901 TGGNGTTGCA CGANTACGTC NNNGCTGCCN NCATAACNNN ANTCGANCNN NNNNCATGTA
          961 ATNANNTANG NNNCNNNNAC NTTNNNNNCN NNCCCCNNNN NNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB18572_B0J5g.seq check
          >D07393A6_B0J5_2_Amp_R_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNA NCANTTATNN GGGTTATTGT CTCNTGAGCG GANACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT TTTAATATAA
            181 ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG TTTTTGTTTT
            241 CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA TCTTGCTCTT
            301 CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG ACCACACACG
            361 AAAATCCTGT GATTTTACAT TTTACTTATC GTTAATCGAA TGTATATCTA TTTAATCTGC
            421 TTTTCTTGTC TAATAAATAT ATATGTAAAG TACGCTTTTT GTTGAAATTT TTTAAACCTT
            481 TGTTTATTTT TTTTTCTTCA TTCCGTAACT CTTCTACCTT CTTTATTTAC TTTCTAAAAT
            541 CCAAATACAA AACATAAAAA TAAATAAACA CAGAGTAAAT TCCCAAATTA TTCCATCATT
            601 AAAAGATACG AGGCGCGTGT AAGTTACAGG CAAGCGATCC GTCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
            781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA NGGCGCGNCA GCGGGTGTTG GCGGGNGTCG
            841 GGGCTGGCTT ANCTATGCGG CATCAGANCA NANTGTACTG ANAGTGNACC ATANCACCTT
            901 TTCNNTTCAT CATTTTTTTT TTANNCTTTT TTTTGANTNN NNNTNNNNTN NAAATTTTTT
          961 TGNNTCNGNN NNCNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl