Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL17 pRS315-GPDp-Ifa38-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: endoplasmic reticulum.

Sequence (full)	RDB18571zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18571_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18571_B0J5a.seq
>D07393A5_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNC GCCNGGGTTT TCCCAGTCAC GACGTTGTAA    61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC   121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA   181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA   241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG   301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC   361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG   421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT   481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA   541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG   601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA   661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT   721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGAC   781 TTTTATGCAA CAGCTTCAAG ANGCTGGGGA ANGANTTAGG TGTATCAATG GTCTTTTATG   841 GNTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTANAT TTTTTAGCTC   901 TTATTTTTGA TCTTTTTTTT ACTACCAGCA GNCNANNTTC GANNNNNNTN GNGCTNAAAC 961 TGGNAAATNC TNNNCNATCA NNNGNNGCNN NNNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3') Sequence file: RDB18571_B0J5b.seq
>D07393A5_B0J5_3_SV40pA3'ElementReverse_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNGNNCCN ANGNNNNNGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC    61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA   121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG   181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG   241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA   301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT   361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG   421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT   481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT   541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA   601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG   661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC   721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC   781 TTACTTTTCG TTGGGATCTT TCGAAAGGAC AGTTTGTGTC GACAGGTAAT GGTTGTCTGG   841 TAAAAGGACA GGGCCATCGC CNATTGGANT ATTTTGTTGA TNATGGNCTG CTANTTGAAN   901 GGAACCATCT NNNNNGNNNN GNCNAANNNN GAANTTAGCN TTGANTNCNT TCTTTTNNTT 961 GNCNGNCNNN NNGNNNNNNN NNN //
Primer: paGFP-M_R1 (Pr0893) Region: insert mid (GFP1-10 5', Ifa38 3') Sequence file: RDB18571_B0J5c.seq
>D07393A5_B0J5_3_paGFP-M_R1_D03_12_ABI24.ab1     1 NNNNNNNNNN NNANNCNTGC CTTTTNNTGT GATCCGGANN NACGGGAAAA GCATTGAACA    61 CCATAGGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA   121 ATAAATTTAA GTGTGAGTTT TCCGATTGTA GCATCACCTT CACCCTCTCC TCTGACAGAA   181 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA TTGGGACAAC TCCAGTGAAA   241 AGTTCTTCTC CTTTGCTCAT ACTAGTGCCA CCCATTGATC CGCCACCAGA CCCTCCGGAT   301 CCTTCCTTTT TAACCTGTCT TGCGGCTTTT TTTAAGGCTC TAATTCTGAT AGATTTATGG   361 AAGGAATAAT TAATGGAGTT AACAATCTTA GAGTAAACGC CAAAGGTCTC TGTAATTACA   421 AATTGATACA CTGCGTGCGC CCAGTAAGGG GTCATAGTAG CGTATCTTTC TTGAGAGCCG   481 CAGCGTCTGC CAACGCTTCT TAAAGTGGAT TTTACAAACT GTTGTGGATT TGGGATCATT   541 AAAGATGATC TTCTGATTTT AGACATCGAG CTAGTGACCA AATATGAAAT GATTAATTCA   601 ACATCGATAG CATCTTTAGA TAATTCCCCA GCCAAAGAGT TAGACCAACC TTGTAAGAAT   661 GATTTCGAAC CACTGTATGT AGCCAAAAGT GGGGTGGGAA TCAGACCACC AAATGAGCCC   721 ATGGTTAAGA TCAAACCACG AGTACCCGAC TTCTTGTTTT CAGCTTTCAC AGTTTCCACA   781 ATCTTTGGTG CAATGATTTG TGTAATTAAT AATGTANCAG TGTTATTGAT AGTGATAATA   841 TTTCTAAGCT CCTTCTCTTC TGTTTCCAAA AATGGAACAG GANTNNAGTG TGATTGANNN   901 ACATTATTGA NCNAAACGGT GATNNGNAAC TGCGCACACA ATTCCTTNAN NNNNTCATAN 961 TTGNNNTCTT TATCCNNCGC NANGNCNNNG GCTANNNNNN TTNNNNNNNN NN //
Primer: LEU2pro_F (Pr0169) Region: LEU2 pro, LEU2 Sequence file: RDB18571_B0J5d.seq
>D07393A5_B0J5_3_LEU2pro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNATT NANGGNNCTA TTGTTTTTTC NNATAGGTGG TTAGCAATCG    61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGANGAAA CCATCAAGAA CGAATTCCCT   781 ACATTGAANG NTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA NTGGTATTAT AATCACCAGC AACATGNTTG GTGANATCAT CTCCNATGAA   901 GCCTCCGTNA TCCCANGNTC CTNNGGNTNG NTGCCNTCNG CNNCCNNGGN CNCTTTGCCN   961 GANAAGAANN CCGCATTTNG NTTNGNNCNA NNCNNTGNNN NNGNNNCNGN TNNNNNNTTN 1021 NCNNAANNNN NNNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18571_B0J5e.seq
>D07393A5_B0J5_3_TDH3pro_F_E03_15_ABI24.ab1     1 NNNNNNNNNN NGNNNTGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA    61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG   121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG   181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT   241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG   301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG   361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT   421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA   481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT   541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT   601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT   661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT   721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC   781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG   841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GNCACTAGCT   901 CGATGNCTAA AATCNNAANA TCATCTTTAA TGATCCNNAA NNCNNNANNN NTTGNAAAAT 961 CCANTTTNAN NNNNNNNNGN NANNNNNNNN NN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10) Sequence file: RDB18571_B0J5f.seq
>D07393A5_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT   121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG   181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT   241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA   301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT   361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA   421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT   481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG   541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT   601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT   661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA   721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC   781 CAGANCCTCC GGANCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAN GNTCTAATTC   841 TGATAGATTT ATGGAAGGAA TAATTAATGN ANTTAACAAT CNNANANTAA ANGCCAANNN   901 TCTCTGTAAT TACNAATNNA TACNCTGCGN GCGCCCANNA ANGGGNCATA NNANCGNATC 961 TTTCTTGANA NCNNCANCNN CNGNNNNCGC TTNNNNAAGN NNNNTTNNNN NNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18571_B0J5g.seq
>D07393A5_B0J5_3_Amp_R_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGAN NANTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCCTAAGAAA CCATTATTAT   661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG   721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA   781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGNGTCG   841 GGGCTGGCTT AACTATGCGG CATCNNANCA GATNGTACTG ANAGTGNACC ATATCGACNA   901 CNTCGTTAAN GNCGTTTCTG ANANANTAAA ANTNTTGANG GAACTTTCNC NNTTANNGGN   961 AANGNNTCNN GNNNNNNNTG ANTNNAACTN NNNCNANNNN NCNNNNCNNN NNNNNNTTTT 1021 NNNTNNNNNN NNTTTTTTTT TTTTTTTNNN ANNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl