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pSFL17 pRS315-GPDp-Ifa38-GFP1-10

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus.

Catalog number RDB18571
Resource name pSFL17 pRS315-GPDp-Ifa38-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS315 (plasmid)
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae IFA38 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18571 pSFL17 pRS315-GPDp-Ifa38-GFP1-10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18571zzt01.seq compiled by us

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18571_B0J5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
          Sequence file: RDB18571_B0J5a.seq check
          >D07393A5_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNC GCCNGGGTTT TCCCAGTCAC GACGTTGTAA
             61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
            121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
            181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
            241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
            301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
            361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
            421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
            481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
            541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
            601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA
            661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT
            721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGAC
            781 TTTTATGCAA CAGCTTCAAG ANGCTGGGGA ANGANTTAGG TGTATCAATG GTCTTTTATG
            841 GNTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTANAT TTTTTAGCTC
            901 TTATTTTTGA TCTTTTTTTT ACTACCAGCA GNCNANNTTC GANNNNNNTN GNGCTNAAAC
          961 TGGNAAATNC TNNNCNATCA NNNGNNGCNN NNNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
          Sequence file: RDB18571_B0J5b.seq check
          >D07393A5_B0J5_3_SV40pA3'ElementReverse_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNGNNCCN ANGNNNNNGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
            301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
            361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
            421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
            481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT
            541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA
            601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG
            661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC
            721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
            781 TTACTTTTCG TTGGGATCTT TCGAAAGGAC AGTTTGTGTC GACAGGTAAT GGTTGTCTGG
            841 TAAAAGGACA GGGCCATCGC CNATTGGANT ATTTTGTTGA TNATGGNCTG CTANTTGAAN
            901 GGAACCATCT NNNNNGNNNN GNCNAANNNN GAANTTAGCN TTGANTNCNT TCTTTTNNTT
          961 GNCNGNCNNN NNGNNNNNNN NNN
          //
          Primer: paGFP-M_R1
          Region: insert mid (GFP1-10 5', Ifa38 3')
          Sequence file: RDB18571_B0J5c.seq check
          >D07393A5_B0J5_3_paGFP-M_R1_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNANNCNTGC CTTTTNNTGT GATCCGGANN NACGGGAAAA GCATTGAACA
             61 CCATAGGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA
            121 ATAAATTTAA GTGTGAGTTT TCCGATTGTA GCATCACCTT CACCCTCTCC TCTGACAGAA
            181 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA TTGGGACAAC TCCAGTGAAA
            241 AGTTCTTCTC CTTTGCTCAT ACTAGTGCCA CCCATTGATC CGCCACCAGA CCCTCCGGAT
            301 CCTTCCTTTT TAACCTGTCT TGCGGCTTTT TTTAAGGCTC TAATTCTGAT AGATTTATGG
            361 AAGGAATAAT TAATGGAGTT AACAATCTTA GAGTAAACGC CAAAGGTCTC TGTAATTACA
            421 AATTGATACA CTGCGTGCGC CCAGTAAGGG GTCATAGTAG CGTATCTTTC TTGAGAGCCG
            481 CAGCGTCTGC CAACGCTTCT TAAAGTGGAT TTTACAAACT GTTGTGGATT TGGGATCATT
            541 AAAGATGATC TTCTGATTTT AGACATCGAG CTAGTGACCA AATATGAAAT GATTAATTCA
            601 ACATCGATAG CATCTTTAGA TAATTCCCCA GCCAAAGAGT TAGACCAACC TTGTAAGAAT
            661 GATTTCGAAC CACTGTATGT AGCCAAAAGT GGGGTGGGAA TCAGACCACC AAATGAGCCC
            721 ATGGTTAAGA TCAAACCACG AGTACCCGAC TTCTTGTTTT CAGCTTTCAC AGTTTCCACA
            781 ATCTTTGGTG CAATGATTTG TGTAATTAAT AATGTANCAG TGTTATTGAT AGTGATAATA
            841 TTTCTAAGCT CCTTCTCTTC TGTTTCCAAA AATGGAACAG GANTNNAGTG TGATTGANNN
            901 ACATTATTGA NCNAAACGGT GATNNGNAAC TGCGCACACA ATTCCTTNAN NNNNTCATAN
          961 TTGNNNTCTT TATCCNNCGC NANGNCNNNG GCTANNNNNN TTNNNNNNNN NN
          //
          Primer: LEU2pro_F
          Region: LEU2 pro, LEU2
          Sequence file: RDB18571_B0J5d.seq check
          >D07393A5_B0J5_3_LEU2pro_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNATT NANGGNNCTA TTGTTTTTTC NNATAGGTGG TTAGCAATCG
             61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
            121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
            181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
            241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
            301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
            361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
            421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
            481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
            541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
            601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
            661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
            721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGANGAAA CCATCAAGAA CGAATTCCCT
            781 ACATTGAANG NTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
            841 ACCCACCTAA NTGGTATTAT AATCACCAGC AACATGNTTG GTGANATCAT CTCCNATGAA
            901 GCCTCCGTNA TCCCANGNTC CTNNGGNTNG NTGCCNTCNG CNNCCNNGGN CNCTTTGCCN
            961 GANAAGAANN CCGCATTTNG NTTNGNNCNA NNCNNTGNNN NNGNNNCNGN TNNNNNNTTN
          1021 NCNNAANNNN NNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB18571_B0J5e.seq check
          >D07393A5_B0J5_3_TDH3pro_F_E03_15_ABI24.ab1
              1 NNNNNNNNNN NGNNNTGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
             61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
            121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
            181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
            241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
            301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
            361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
            421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
            481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
            541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
            601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
            661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
            721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
            781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG
            841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GNCACTAGCT
            901 CGATGNCTAA AATCNNAANA TCATCTTTAA TGATCCNNAA NNCNNNANNN NTTGNAAAAT
          961 CCANTTTNAN NNNNNNNNGN NANNNNNNNN NN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, GFP1-10)
          Sequence file: RDB18571_B0J5f.seq check
          >D07393A5_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
             61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
            121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
            181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
            241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
            301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
            361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
            421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
            481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
            541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
            601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
            661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
            721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
            781 CAGANCCTCC GGANCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAN GNTCTAATTC
            841 TGATAGATTT ATGGAAGGAA TAATTAATGN ANTTAACAAT CNNANANTAA ANGCCAANNN
            901 TCTCTGTAAT TACNAATNNA TACNCTGCGN GCGCCCANNA ANGGGNCATA NNANCGNATC
          961 TTTCTTGANA NCNNCANCNN CNGNNNNCGC TTNNNNAAGN NNNNTTNNNN NNNNNN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB18571_B0J5g.seq check
          >D07393A5_B0J5_3_Amp_R_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTGAN NANTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
            181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
            241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
            301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
            361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
            421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
            481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
            541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
            601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCCTAAGAAA CCATTATTAT
            661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
            721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
            781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGNGTCG
            841 GGGCTGGCTT AACTATGCGG CATCNNANCA GATNGTACTG ANAGTGNACC ATATCGACNA
            901 CNTCGTTAAN GNCGTTTCTG ANANANTAAA ANTNTTGANG GAACTTTCNC NNTTANNGGN
            961 AANGNNTCNN GNNNNNNNTG ANTNNAACTN NNNCNANNNN NCNNNNCNNN NNNNNNTTTT
          1021 NNNTNNNNNN NNTTTTTTTT TTTTTTTNNN ANNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.16

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