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pSFL17 pRS315-GPDp-Ifa38-GFP1-10

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus.

Catalog number RDB18571
Resource name pSFL17 pRS315-GPDp-Ifa38-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS315 (plasmid)
Selectable markers Ampicillin (E. coli), LEU2 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18571 pSFL17 pRS315-GPDp-Ifa38-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18571_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
Sequence file: RDB18571_B0J5a.seq check
>D07393A5_B0J5_3_HB-NOS_R_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN NNNNNGTNNC GCCNGGGTTT TCCCAGTCAC GACGTTGTAA
   61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
  121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
  181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
  241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
  301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
  361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
  421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
  481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
  541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
  601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA
  661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT
  721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCATGAC
  781 TTTTATGCAA CAGCTTCAAG ANGCTGGGGA ANGANTTAGG TGTATCAATG GTCTTTTATG
  841 GNTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTANAT TTTTTAGCTC
  901 TTATTTTTGA TCTTTTTTTT ACTACCAGCA GNCNANNTTC GANNNNNNTN GNGCTNAAAC
961 TGGNAAATNC TNNNCNATCA NNNGNNGCNN NNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB18571_B0J5b.seq check
>D07393A5_B0J5_3_SV40pA3'ElementReverse_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNCCN ANGNNNNNGC NNGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
  301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
  361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  421 CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
  481 ACCGGGCCCG GCCGCAAATT AAAGCCTTCG AGCGTCCCAA AACCTTCTCA AGCAAGGTTT
  541 TCAGTATAAT GTTACATGCG TACACGCGTC TGTACAGAAA AAAAAGAAAA ATTTGAAATA
  601 TAAATAACGT TCTTAATACT AACATAACTA TAAAAAAATA AATAGGGACC TAGACTTCAG
  661 GTTGTCTAAC TCCTTCCTTT TCGGTTAGAG CGGATGTGGG GGGAGGGCGT GAATGTAAGC
  721 GTGACATAAC TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
  781 TTACTTTTCG TTGGGATCTT TCGAAAGGAC AGTTTGTGTC GACAGGTAAT GGTTGTCTGG
  841 TAAAAGGACA GGGCCATCGC CNATTGGANT ATTTTGTTGA TNATGGNCTG CTANTTGAAN
  901 GGAACCATCT NNNNNGNNNN GNCNAANNNN GAANTTAGCN TTGANTNCNT TCTTTTNNTT
961 GNCNGNCNNN NNGNNNNNNN NNN
//
Primer: paGFP-M_R1
Region: insert mid (GFP1-10 5', Ifa38 3')
Sequence file: RDB18571_B0J5c.seq check
>D07393A5_B0J5_3_paGFP-M_R1_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNANNCNTGC CTTTTNNTGT GATCCGGANN NACGGGAAAA GCATTGAACA
   61 CCATAGGTCA GAGTAGTGAC AAGTGTTGGC CATGGAACAG GTAGTTTTCC AGTAGTGCAA
  121 ATAAATTTAA GTGTGAGTTT TCCGATTGTA GCATCACCTT CACCCTCTCC TCTGACAGAA
  181 AATTTGTGCC CATTAACATC ACCATCTAAT TCAACAAGAA TTGGGACAAC TCCAGTGAAA
  241 AGTTCTTCTC CTTTGCTCAT ACTAGTGCCA CCCATTGATC CGCCACCAGA CCCTCCGGAT
  301 CCTTCCTTTT TAACCTGTCT TGCGGCTTTT TTTAAGGCTC TAATTCTGAT AGATTTATGG
  361 AAGGAATAAT TAATGGAGTT AACAATCTTA GAGTAAACGC CAAAGGTCTC TGTAATTACA
  421 AATTGATACA CTGCGTGCGC CCAGTAAGGG GTCATAGTAG CGTATCTTTC TTGAGAGCCG
  481 CAGCGTCTGC CAACGCTTCT TAAAGTGGAT TTTACAAACT GTTGTGGATT TGGGATCATT
  541 AAAGATGATC TTCTGATTTT AGACATCGAG CTAGTGACCA AATATGAAAT GATTAATTCA
  601 ACATCGATAG CATCTTTAGA TAATTCCCCA GCCAAAGAGT TAGACCAACC TTGTAAGAAT
  661 GATTTCGAAC CACTGTATGT AGCCAAAAGT GGGGTGGGAA TCAGACCACC AAATGAGCCC
  721 ATGGTTAAGA TCAAACCACG AGTACCCGAC TTCTTGTTTT CAGCTTTCAC AGTTTCCACA
  781 ATCTTTGGTG CAATGATTTG TGTAATTAAT AATGTANCAG TGTTATTGAT AGTGATAATA
  841 TTTCTAAGCT CCTTCTCTTC TGTTTCCAAA AATGGAACAG GANTNNAGTG TGATTGANNN
  901 ACATTATTGA NCNAAACGGT GATNNGNAAC TGCGCACACA ATTCCTTNAN NNNNTCATAN
961 TTGNNNTCTT TATCCNNCGC NANGNCNNNG GCTANNNNNN TTNNNNNNNN NN
//
Primer: LEU2pro_F
Region: LEU2 pro, LEU2
Sequence file: RDB18571_B0J5d.seq check
>D07393A5_B0J5_3_LEU2pro_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNATT NANGGNNCTA TTGTTTTTTC NNATAGGTGG TTAGCAATCG
   61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGANGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAANG NTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA NTGGTATTAT AATCACCAGC AACATGNTTG GTGANATCAT CTCCNATGAA
  901 GCCTCCGTNA TCCCANGNTC CTNNGGNTNG NTGCCNTCNG CNNCCNNGGN CNCTTTGCCN
  961 GANAAGAANN CCGCATTTNG NTTNGNNCNA NNCNNTGNNN NNGNNNCNGN TNNNNNNTTN
1021 NCNNAANNNN NNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB18571_B0J5e.seq check
>D07393A5_B0J5_3_TDH3pro_F_E03_15_ABI24.ab1
    1 NNNNNNNNNN NGNNNTGNNT GNNNTTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG
  121 GCCGCATGAC TTTTATGCAA CAGCTTCAAG AGGCTGGGGA AAGATTTAGG TGTATCAATG
  181 GTCTTTTATG GGTTGTTTTT GGGCTTGGTG TCTTAAAATG TACAACGTTA TCACTTAGAT
  241 TTTTAGCTCT TATTTTTGAT CTTTTTTTAC TACCAGCAGT CAATTTCGAC AAGTATGGTG
  301 CTAAAACTGG TAAATACTGT GCTATCACTG GTGCAAGTGA CGGTATTGGT AAAGAATTTG
  361 CTAGACAAAT GGCGAAACGT GGCTTCAACT TGGTATTGAT CTCGAGAACG CAATCTAAAT
  421 TGGAGGCTTT ACAAAAAGAA CTAGAAGATC AACATCATGT CGTTGTAAAG ATTCTAGCCA
  481 TTGACATTGC GGAGGATAAA GAATCCAACT ATGAATCCAT TAAGGAATTG TGTGCGCAGT
  541 TACCAATCAC CGTTTTGGTC AATAATGTTG GTCAATCACA CTCCATTCCT GTTCCATTTT
  601 TGGAAACAGA AGAGAAGGAG CTTAGAAATA TTATCACTAT CAATAACACT GCTACATTAT
  661 TAATTACACA AATCATTGCA CCAAAGATTG TGGAAACTGT GAAAGCTGAA AACAAGAAGT
  721 CGGGTACTCG TGGTTTGATC TTAACCATGG GCTCATTTGG TGGTCTGATT CCCACCCCAC
  781 TTTTGGCTAC ATACAGTGGT TCGAAATCAT TCTTACAAGG TTGGTCTAAC TCTTTGGCTG
  841 GGGAATTATC TAAAGATGCT ATCGATGTTG AATTAATCAT TTCATATTTG GNCACTAGCT
  901 CGATGNCTAA AATCNNAANA TCATCTTTAA TGATCCNNAA NNCNNNANNN NTTGNAAAAT
961 CCANTTTNAN NNNNNNNNGN NANNNNNNNN NN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10)
Sequence file: RDB18571_B0J5f.seq check
>D07393A5_B0J5_3_CYC1ter_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGACATAACT
   61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTTTCGT
  121 TGGGATCTTT CGAAAGGACA GTTTGTGTCG ACAGGTAATG GTTGTCTGGT AAAAGGACAG
  181 GGCCATCGCC AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG GAACCATCTT
  241 CAACGTTGTG GCGAACTGTG AAGTTAGCTT TGATTCCATT CTTTTGTTTG TCTGCCGTGA
  301 TGTATACATT GTGTGAGTTA AAGTTGTACT CGAGTTTGTG TCCGAGAATG TTTCCATCTT
  361 CTTTAAAATC AGTACCCTTT AACTCGATAC GATTAACAAG GGTATCACCT TCAAACTTGA
  421 CTACAGCACG CGTCTTGTAT TTCCCGTCAT CTTTGAAAGA TATAGTGCGT TCCTGTACAT
  481 AACCTTCGGG CATGGCACTC TTGAAAAAGT CATGCCTTTT CATGTGATCC GGATAACGGG
  541 AAAAGCATTG AACACCATAG GTCAGAGTAG TGACAAGTGT TGGCCATGGA ACAGGTAGTT
  601 TTCCAGTAGT GCAAATAAAT TTAAGTGTGA GTTTTCCGAT TGTAGCATCA CCTTCACCCT
  661 CTCCTCTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA
  721 CAACTCCAGT GAAAAGTTCT TCTCCTTTGC TCATACTAGT GCCACCCATT GATCCGCCAC
  781 CAGANCCTCC GGANCCTTCC TTTTTAACCT GTCTTGCGGC TTTTTTTAAN GNTCTAATTC
  841 TGATAGATTT ATGGAAGGAA TAATTAATGN ANTTAACAAT CNNANANTAA ANGCCAANNN
  901 TCTCTGTAAT TACNAATNNA TACNCTGCGN GCGCCCANNA ANGGGNCATA NNANCGNATC
961 TTTCTTGANA NCNNCANCNN CNGNNNNCGC TTNNNNAAGN NNNNTTNNNN NNNNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18571_B0J5g.seq check
>D07393A5_B0J5_3_Amp_R_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGAN NANTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCCTAAGAAA CCATTATTAT
  661 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
  721 TGANGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAN ACGGTCACAG CTTGTCTGTA
  781 AGCGGANGCC GGGANCAGAN AAGCCCGTCA GGGCGCGTCA GCGGGNGTTG GCGGGNGTCG
  841 GGGCTGGCTT AACTATGCGG CATCNNANCA GATNGTACTG ANAGTGNACC ATATCGACNA
  901 CNTCGTTAAN GNCGTTTCTG ANANANTAAA ANTNTTGANG GAACTTTCNC NNTTANNGGN
  961 AANGNNTCNN GNNNNNNNTG ANTNNAACTN NNNCNANNNN NCNNNNCNNN NNNNNNTTTT
1021 NNNTNNNNNN NNTTTTTTTT TTTTTTTNNN ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl