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pSFL12 pRS314-GPDp-GFP11

Expression vector of the the last 11th beta-strand (GFP11).

Catalog number RDB18570
Resource name pSFL12 pRS314-GPDp-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of the the last 11th beta-strand (GFP11), GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB18570 pSFL12 pRS314-GPDp-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18570_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB18570_B0J5a.seq check
>D07393A4_B0J5_2_HB-NOS_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNNNNGTAN NGCCNGGGTT TTCCCNGTCN CGACGTTGTA
   61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT
  121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
  181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
  241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
  301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
  361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
  421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
  481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
  541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA
  601 GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT
  661 AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG
  721 TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCTCTA
  781 GAACTAGTGG ATCCGGANGG TCTGGTGGCG GATCAACAAG TCGTGACCAC ATGNNCCTTC
  841 ATGANTACGT AAATGCTGCT GGGANTANAT AAGAATTCGA TATCNAGCTT ATCGATANCG
  901 NCNANCNCNN NNCATGNAAT TAGTTATGNC ACGCTTACAT TCNCGCCCNC CCCCNNCATC
961 CGNNCTNNNC NNAAAANGNA NNNANTTAGA NNNNCNNNNA NNNNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP11)
Sequence file: RDB18570_B0J5b.seq check
>D07393A4_B0J5_2_SV40pA3'ElementReverse_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNNANGCNN GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
  121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
  181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
  241 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
  301 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
  361 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT
  421 ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC
  481 CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC AAAACCTTCT CAAGCAAGGT TTTCAGTATA
  541 ATGTTACATG CGTACACGCG TCTGTACAGA AAAAAAAGAA AAATTTGAAA TATAAATAAC
  601 GTTCTTAATA CTAACATAAC TATAAAAAAA TAAATAGGGA CCTAGACTTC AGGTTGTCTA
  661 ACTCCTTCCT TTTCGGTTAG AGCGGATGTG GGGGGAGGGC GTGAATGTAA GCGTGACATA
  721 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTATGTAA
  781 TCCCAGCAGC ATTTACGTAC TCATGAANGA NCATGTGGTC ACGACTTGTT GATCCGCCNC
  841 CAGACCCTCC GGNTCCACTA GTTCTAGAGC GGCCGCATCN GTCNAAACTA AGTTCNGGNG
  901 TTTTAAAACT AAAAAAAAGA NTAACTATNA AAGTANAANT TNANNNNNTT NNNNNNANAT
961 TTACNNNANN NNNNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, GFP11, CYC1 ter)
Sequence file: RDB18570_B0J5c.seq check
>D07393A4_B0J5_2_TDH3pro_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNNNNCTNN NNNCTATTTC TTAAACTTCT TAAATTCTAC
   61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
  121 CCGCTCTAGA ACTAGTGGAT CCGGAGGGTC TGGTGGCGGA TCAACAAGTC GTGACCACAT
  181 GGTCCTTCAT GAGTACGTAA ATGCTGCTGG GATTACATAA GAATTCGATA TCAAGCTTAT
  241 CGATACCGTC GACCTCGAGT CATGTAATTA GTTATGTCAC GCTTACATTC ACGCCCTCCC
  301 CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA GGTCCCTATT
  361 TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT TTTTCTTTTT
  421 TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT TGAGAAGGTT
  481 TTGGGACGCT CGAAGGCTTT AATTTGCGGC CGGTACCAGC TTTTGTTCCC TTTAGTGAGG
  541 GTTAATTTCG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
  601 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
  661 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
  721 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGNGAGGCG GTTTGCGTAT
  781 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
  841 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC NCAGAATCAG GGGANAACGC
  901 NNNAAGAACA TGTGANCNAA NNGNNAGCAA AANNNNNGGA ACCGNAAAAA NNNNCGNGTT
  961 GCTGGNGNTT TTTCCNNNNN NNNCNNNCNN CNNGANNNNC ATNNNNAAAA NNNNNNNCNN
1021 NNNNNNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB18570_B0J5d.seq check
>D07393A4_B0J5_2_TRP1pro_F_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN AAGGCNGCTN GGNNTNNGTC TGTTATTAAT TTCNCAGGTA
   61 GTTCTGGTCC NTTGGTGAAA GTTTGCGGCT TGCAGAGCAC AGAGGCCGCA GAATGTGCTC
  121 TAGATTCCGA TGCTGACTTG CTGGGTATTA TATGTGTGCC CAATAGAAAG AGAACAATTG
  181 ACCCGGTTAT TGCAAGGAAA ATTTCAAGTC TTGTAAAAGC ATATAAAAAT AGTTCAGGCA
  241 CTCCGAAATA CTTGGTTGGC GTGTTTCGTA ATCAACCTAA GGAGGATGTT TTGGCTCTGG
  301 TCAATGATTA CGGCATTGAT ATCGTCCAAC TGCATGGAGA TGAGTCGTGG CAAGAATACC
  361 AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT TCCAAAAGAC TGCAACATAC
  421 TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC CTTGTTTGAT TCAGAAGCAG
  481 GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA CTGGGTTGGA AGGCAAGAGA
  541 GCCCCGAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG
  601 CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG
  661 TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG
  721 AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCGATC TATGCGGTGT GAAATACCGC
  781 ACAGATGCGT AANGAGAAAA TACCGCATCA NGAAATTGTA AGCGTTAATA TTTTGTTAAA
  841 ATTCGCGTTA AATTTNTGTT AANTCAGCTC ATTTTTTANC CNATANGCNN AAATCNGNCA
  901 AAATCCCTTA TAAATCAAAA GNNTAGACCN AGATANGNNT NANTGNNGNT NNNNNTTTGG
961 NNNNNNANTC CACTATNNNN NNNNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18570_B0J5e.seq check
>D07393A4_B0J5_2_Amp_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNAN CATTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGANGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGNGTTGG NGGGNGTCNG
  841 GGCTGGCTTA ACTATGCGGC ATCANANCAN ANTGTACTGA GAGTGNNNNA TANATCACGA
  901 NATTACTATA TATATAATAT AGGAAGCATT TNATANAACA GCATCGTAAT ATATGTGTAC
  961 TTTGCANTTN TGNNNCCNGA NGGNANTANN NNNNNANATT CNTNNNNGAA AAATANCTTG
1021 NCNNCNTNAN GNANNNNCNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl