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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSFL12 pRS314-GPDp-GFP11

Expression vector of the the last 11th beta-strand (GFP11).

Catalog number RDB18570
Resource name pSFL12 pRS314-GPDp-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of the the last 11th beta-strand (GFP11), GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB18570zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB18570 pSFL12 pRS314-GPDp-GFP11 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualizing multiple inter-organelle contact sites (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18570_B0J5p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: HB-NOS_R (Pr0800)
            Region: T7 pro, insert 5' (GPD pro)
            Sequence file: RDB18570_B0J5a.seq check
            >D07393A4_B0J5_2_HB-NOS_R_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNNNNGTAN NGCCNGGGTT TTCCCNGTCN CGACGTTGTA
               61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT
              121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
              181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
              241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
              301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
              361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
              421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
              481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
              541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA
              601 GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT
              661 AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG
              721 TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCTCTA
              781 GAACTAGTGG ATCCGGANGG TCTGGTGGCG GATCAACAAG TCGTGACCAC ATGNNCCTTC
              841 ATGANTACGT AAATGCTGCT GGGANTANAT AAGAATTCGA TATCNAGCTT ATCGATANCG
              901 NCNANCNCNN NNCATGNAAT TAGTTATGNC ACGCTTACAT TCNCGCCCNC CCCCNNCATC
            961 CGNNCTNNNC NNAAAANGNA NNNANTTAGA NNNNCNNNNA NNNNNNNNNN
            //
            Primer: SV40pA3'Element Reverse (Pr0108)
            Region: T3 pro, insert 3' (CYC1 ter, GFP11)
            Sequence file: RDB18570_B0J5b.seq check
            >D07393A4_B0J5_2_SV40pA3'ElementReverse_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG GNNNANGCNN GCAACGCGGC CTTTTTACGG TTCCTGGCCT
               61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
              121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
              181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
              241 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
              301 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
              361 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT
              421 ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC
              481 CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC AAAACCTTCT CAAGCAAGGT TTTCAGTATA
              541 ATGTTACATG CGTACACGCG TCTGTACAGA AAAAAAAGAA AAATTTGAAA TATAAATAAC
              601 GTTCTTAATA CTAACATAAC TATAAAAAAA TAAATAGGGA CCTAGACTTC AGGTTGTCTA
              661 ACTCCTTCCT TTTCGGTTAG AGCGGATGTG GGGGGAGGGC GTGAATGTAA GCGTGACATA
              721 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTATGTAA
              781 TCCCAGCAGC ATTTACGTAC TCATGAANGA NCATGTGGTC ACGACTTGTT GATCCGCCNC
              841 CAGACCCTCC GGNTCCACTA GTTCTAGAGC GGCCGCATCN GTCNAAACTA AGTTCNGGNG
              901 TTTTAAAACT AAAAAAAAGA NTAACTATNA AAGTANAANT TNANNNNNTT NNNNNNANAT
            961 TTACNNNANN NNNNNNNN
            //
            Primer: TDH3pro_F (Pr0431)
            Region: insert mid (GPD pro, GFP11, CYC1 ter)
            Sequence file: RDB18570_B0J5c.seq check
            >D07393A4_B0J5_2_TDH3pro_F_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT GNNNNNCTNN NNNCTATTTC TTAAACTTCT TAAATTCTAC
               61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
              121 CCGCTCTAGA ACTAGTGGAT CCGGAGGGTC TGGTGGCGGA TCAACAAGTC GTGACCACAT
              181 GGTCCTTCAT GAGTACGTAA ATGCTGCTGG GATTACATAA GAATTCGATA TCAAGCTTAT
              241 CGATACCGTC GACCTCGAGT CATGTAATTA GTTATGTCAC GCTTACATTC ACGCCCTCCC
              301 CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA GGTCCCTATT
              361 TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT TTTTCTTTTT
              421 TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT TGAGAAGGTT
              481 TTGGGACGCT CGAAGGCTTT AATTTGCGGC CGGTACCAGC TTTTGTTCCC TTTAGTGAGG
              541 GTTAATTTCG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC
              601 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA
              661 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
              721 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGNGAGGCG GTTTGCGTAT
              781 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
              841 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC NCAGAATCAG GGGANAACGC
              901 NNNAAGAACA TGTGANCNAA NNGNNAGCAA AANNNNNGGA ACCGNAAAAA NNNNCGNGTT
              961 GCTGGNGNTT TTTCCNNNNN NNNCNNNCNN CNNGANNNNC ATNNNNAAAA NNNNNNNCNN
            1021 NNNNNNNN
            //
            Primer: TRP1pro_F (Pr0171)
            Region: TRP1
            Sequence file: RDB18570_B0J5d.seq check
            >D07393A4_B0J5_2_TRP1pro_F_B02_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN AAGGCNGCTN GGNNTNNGTC TGTTATTAAT TTCNCAGGTA
               61 GTTCTGGTCC NTTGGTGAAA GTTTGCGGCT TGCAGAGCAC AGAGGCCGCA GAATGTGCTC
              121 TAGATTCCGA TGCTGACTTG CTGGGTATTA TATGTGTGCC CAATAGAAAG AGAACAATTG
              181 ACCCGGTTAT TGCAAGGAAA ATTTCAAGTC TTGTAAAAGC ATATAAAAAT AGTTCAGGCA
              241 CTCCGAAATA CTTGGTTGGC GTGTTTCGTA ATCAACCTAA GGAGGATGTT TTGGCTCTGG
              301 TCAATGATTA CGGCATTGAT ATCGTCCAAC TGCATGGAGA TGAGTCGTGG CAAGAATACC
              361 AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT TCCAAAAGAC TGCAACATAC
              421 TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC CTTGTTTGAT TCAGAAGCAG
              481 GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA CTGGGTTGGA AGGCAAGAGA
              541 GCCCCGAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG
              601 CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG
              661 TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG
              721 AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCGATC TATGCGGTGT GAAATACCGC
              781 ACAGATGCGT AANGAGAAAA TACCGCATCA NGAAATTGTA AGCGTTAATA TTTTGTTAAA
              841 ATTCGCGTTA AATTTNTGTT AANTCAGCTC ATTTTTTANC CNATANGCNN AAATCNGNCA
              901 AAATCCCTTA TAAATCAAAA GNNTAGACCN AGATANGNNT NANTGNNGNT NNNNNTTTGG
            961 NNNNNNANTC CACTATNNNN NNNNNN
            //
            Primer: Amp_R (Pr0008)
            Region: AmpR pro, CEN_ARS
            Sequence file: RDB18570_B0J5e.seq check
            >D07393A4_B0J5_2_Amp_R_E02_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTNAN CATTTATCNG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
              181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
              241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
              301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
              361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
              421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
              481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
              541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
              601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
              661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
              721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
              781 GCGGANGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGNGTTGG NGGGNGTCNG
              841 GGCTGGCTTA ACTATGCGGC ATCANANCAN ANTGTACTGA GAGTGNNNNA TANATCACGA
              901 NATTACTATA TATATAATAT AGGAAGCATT TNATANAACA GCATCGTAAT ATATGTGTAC
              961 TTTGCANTTN TGNNNCCNGA NGGNANTANN NNNNNANATT CNTNNNNGAA AAATANCTTG
            1021 NCNNCNTNAN GNANNNNCNT NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

            2023.04.06

            GNP_filter3_RDBDEP_html_230405.pl