Please review the QC test results indicated by icon below as well as clone information before placing your order.

pSFL10 pRS314-GPDp-GFP1-10

Expression vector of the first ten beta-strands (GFP1-10).

Sequence (full)	RDB18568zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18568_B0J5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB18568_B0J5a.seq
>D07393A2_B0J5_1_HB-NOS_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGGTNNN GCCNGGGTTT TCCCNGTCAC GACGTTGTAA    61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC   121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA   181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA   241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG   301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC   361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG   421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT   481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA   541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG   601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA   661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT   721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCTCTAG   781 AACTAGTGGA TCCGGANGGT CTGGTGGCGG ATCAATGGGT GGCACTAGTA TGAGCAAAGG   841 ANAAGAACTT TTCACTGGAN TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG   901 GGCACAAATT TTCTGTCANA NGANANGGTG AANGTGANGC TACNATCNGN AAACTCACAC 961 TTNAATTTAT TTGCNCTACN NNNAAANTAN NNNNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3') Sequence file: RDB18568_B0J5b.seq
>D07393A2_B0J5_1_SV40pA3'ElementReverse_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTG GNNNNNGCNN GCAACGCGGC CTTTTTACGG TTCCTGGCCT    61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC   121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG   181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT   241 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC   301 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC   361 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT   421 ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC   481 CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC AAAACCTTCT CAAGCAAGGT TTTCAGTATA   541 ATGTTACATG CGTACACGCG TCTGTACAGA AAAAAAAGAA AAATTTGAAA TATAAATAAC   601 GTTCTTAATA CTAACATAAC TATAAAAAAA TAAATAGGGA CCTAGACTTC AGGTTGTCTA   661 ACTCCTTCCT TTTCGGTTAG AGCGGATGTG GGGGGAGGGC GTGAATGTAA GCGTGACATA   721 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTACTTTT   781 CGTTGGGATC TTTCGAAAGG ACAGTTTGTG TCGACAGGTA ATGGTTGTCT GGTAAAANGA   841 CANGGCCATC GCCAATTGGA NTATTTTGTT GATAATGGNC TGCTANTTGA ANNNANNCAT   901 CTTCNNCGNN NNNGCGAANN NNGAANTTAN CTTTNANNCC ATTCTTTTGN TTGNCNGNCN 961 NGATGNATAC ATTNNGNNAN TNNAANNNNN NNNNN //
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, GFP1-10 5') Sequence file: RDB18568_B0J5c.seq
>D07393A2_B0J5_1_TDH3pro_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NGNNNTGNNT GNANTTCTNN NNNCTATTTC TTAAACTTCT TAAATTCTAC    61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG   121 CCGCTCTAGA ACTAGTGGAT CCGGAGGGTC TGGTGGCGGA TCAATGGGTG GCACTAGTAT   181 GAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA   241 TGTTAATGGG CACAAATTTT CTGTCAGAGG AGAGGGTGAA GGTGATGCTA CAATCGGAAA   301 ACTCACACTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACACTTGT   361 CACTACTCTG ACCTATGGTG TTCAATGCTT TTCCCGTTAT CCGGATCACA TGAAAAGGCA   421 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAACGCACTA TATCTTTCAA   481 AGATGACGGG AAATACAAGA CGCGTGCTGT AGTCAAGTTT GAAGGTGATA CCCTTGTTAA   541 TCGTATCGAG TTAAAGGGTA CTGATTTTAA AGAAGATGGA AACATTCTCG GACACAAACT   601 CGAGTACAAC TTTAACTCAC ACAATGTATA CATCACGGCA GACAAACAAA AGAATGGAAT   661 CAAAGCTAAC TTCACAGTTC GCCACAACGT TGAAGATGGT TCCGTTCAAC TAGCAGANCA   721 TTATCAACAA AATACTCCNA TTGGCGATGG NCCTGTCCTT TTACCNGACA ACCATTACCT   781 GTCGACACNA ACTGTCCTTT CGAAAGATCC CAACGAAAAG TAAGAATTCN ATATCNAGCT   841 TATCGATACN GTCGANCTCN AGTCATGTAA TTAGTTATGT CACGCTNANN TTCNCGCCCN   901 CCCCCCCNCA TNNGCNNTNN NCGAAAANGN ANGNANTTAG NNNNNCNNGN ANNNCTNNGN   961 CCCNATTNAT TTTTTTNNNN NNNNNGNNNN NNNTNNNNNN NNTNNTNNNN NNTNNNANNT 1021 TTTNNNTTNN TTNTNNNNNN NNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18568_B0J5d.seq
>D07393A2_B0J5_1_TRP1pro_F_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCAGCTT GGNNTNNGTC TGTTATTAAT TTCNCAGGTA    61 GTTCTGGTCC ATTGGTGAAA GTTTGCGGCT TGCAGAGCAC AGAGGCCGCA GAATGTGCTC   121 TAGATTCCGA TGCTGACTTG CTGGGTATTA TATGTGTGCC CAATAGAAAG AGAACAATTG   181 ACCCGGTTAT TGCAAGGAAA ATTTCAAGTC TTGTAAAAGC ATATAAAAAT AGTTCAGGCA   241 CTCCGAAATA CTTGGTTGGC GTGTTTCGTA ATCAACCTAA GGAGGATGTT TTGGCTCTGG   301 TCAATGATTA CGGCATTGAT ATCGTCCAAC TGCATGGAGA TGAGTCGTGG CAAGAATACC   361 AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT TCCAAAAGAC TGCAACATAC   421 TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC CTTGTTTGAT TCAGAAGCAG   481 GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA CTGGGTTGGA AGGCAAGAGA   541 GCCCCGAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG   601 CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG   661 TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG   721 AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCGATC TATGCGGTGT GAAATACCGC   781 ACAGATGCGT AANGAGAAAA TACCGCATCA NGAAATTGTA AGCGTTAATA TTTTGTTAAA   841 ATTCGCGTTA AATTTTTGTN NAATCAGCTC ATTTTTTNNC CNATANGNCG AAATCGGNAA   901 AATNCCTTAT AAATCAAAAG AATANANCGA NNTAGGGTTN ANNNNNGNTN CCNNTTTGGN 961 NNNNGANTNN NCTANTNANN NNNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, GFP1-10 3') Sequence file: RDB18568_B0J5e.seq
>D07393A2_B0J5_1_CYC1ter_R_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGG NNGNGGGGGG NNGGCGTGAA TGTAAGCGTG ACATAACTAA    61 TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA CTTTTCGTTG   121 GGATCTTTCG AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG   181 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA   241 ACGTTGTGGC GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG   301 TATACATTGT GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT   361 TTAAAATCAG TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT   421 ACAGCACGCG TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA   481 CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ANAACGGGAA   541 AAGCNTTGAA CACCATANGT CANANTAGTG ACNAGTGNTG GNCNTGGAAC AGGTANTTTT   601 CCNGTANNGN AAATAAATTT AAGTGNGANT TTTCCNANNG NANCATCNCC NNCNCCCTCT 661 NCNCNGANAN AAAANTTNNN NCNNNTNNNN TCNNCNTCTN NNNCNNNNNN NNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18568_B0J5f.seq
>D07393A2_B0J5_1_Amp_R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNANTN NNCATTNATN NGGGTTATTG TCTCATGAGC GGATACATAT    61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC   121 CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA   181 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT   241 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT   301 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC   361 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG   421 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT   481 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA   541 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT   601 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCTAAGAA ACCATTATTA   661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG   721 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA NACGGTCACA GCTTGTCTGT   781 AAGCGGANGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC   841 GGGGCTGGNT TAACTATGCG GCATCAGANC ANANTGNACT GAGAGTGNAC CATANNTCAC   901 GANNTTACTA TATATATAAT ATAGGAAGCA TTTNATAGAA CAGCATCNNA ATATATGTGT   961 ACTTTGCANN TATGANGCCA GANGGNAGTA NNNGNAANAN ATTNNTNANN GNAAAANNAN  1021 NNNGNNNNNN TNNCNNNNNN NNNTNNNNNN NGNNNNNTTN NNTTTTTNNN CNNNNNNNNN 1081 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl