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pSFL10 pRS314-GPDp-GFP1-10

Expression vector of the first ten beta-strands (GFP1-10).

Catalog number RDB18568
Resource name pSFL10 pRS314-GPDp-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of the first ten beta-strands (GFP1-10), GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18568 pSFL10 pRS314-GPDp-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18568_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB18568_B0J5a.seq check
>D07393A2_B0J5_1_HB-NOS_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGTNNN GCCNGGGTTT TCCCNGTCAC GACGTTGTAA
   61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTGGAGC TCAGTTTATC
  121 ATTATCAATA CTCGCCATTT CAAAGAATAC GTAAATAATT AATAGTAGTG ATTTTCCTAA
  181 CTTTATTTAG TCAAAAAATT AGCCTTTTAA TTCTGCTGTA ACCCGTACAT GCCCAAAATA
  241 GGGGGCGGGT TACACAGAAT ATATAACATC GTAGGTGTCT GGGTGAACAG TTTATTCCTG
  301 GCATCCACTA AATATAATGG AGCCCGCTTT TTAAGCTGGC ATCCAGAAAA AAAAAGAATC
  361 CCAGCACCAA AATATTGTTT TCTTCACCAA CCATCAGTTC ATAGGTCCAT TCTCTTAGCG
  421 CAACTACAGA GAACAGGGGC ACAAACAGGC AAAAAACGGG CACAACCTCA ATGGAGTGAT
  481 GCAACCTGCC TGGAGTAAAT GATGACACAA GGCAATTGAC CCACGCATGT ATCTATCTCA
  541 TTTTCTTACA CCTTCTATTA CCTTCTGCTC TCTCTGATTT GGAAAAAGCT GAAAAAAAAG
  601 GTTGAAACCA GTTCCCTGAA ATTATTCCCC TACTTGACTA ATAAGTATAT AAAGACGGTA
  661 GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA CTTTTATAGT
  721 TAGTCTTTTT TTTAGTTTTA AAACACCAGA ACTTAGTTTC GACGGATGCG GCCGCTCTAG
  781 AACTAGTGGA TCCGGANGGT CTGGTGGCGG ATCAATGGGT GGCACTAGTA TGAGCAAAGG
  841 ANAAGAACTT TTCACTGGAN TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG
  901 GGCACAAATT TTCTGTCANA NGANANGGTG AANGTGANGC TACNATCNGN AAACTCACAC
961 TTNAATTTAT TTGCNCTACN NNNAAANTAN NNNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB18568_B0J5b.seq check
>D07393A2_B0J5_1_SV40pA3'ElementReverse_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTG GNNNNNGCNN GCAACGCGGC CTTTTTACGG TTCCTGGCCT
   61 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
  121 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
  181 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
  241 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
  301 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC
  361 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT
  421 ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC
  481 CGGCCGCAAA TTAAAGCCTT CGAGCGTCCC AAAACCTTCT CAAGCAAGGT TTTCAGTATA
  541 ATGTTACATG CGTACACGCG TCTGTACAGA AAAAAAAGAA AAATTTGAAA TATAAATAAC
  601 GTTCTTAATA CTAACATAAC TATAAAAAAA TAAATAGGGA CCTAGACTTC AGGTTGTCTA
  661 ACTCCTTCCT TTTCGGTTAG AGCGGATGTG GGGGGAGGGC GTGAATGTAA GCGTGACATA
  721 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTACTTTT
  781 CGTTGGGATC TTTCGAAAGG ACAGTTTGTG TCGACAGGTA ATGGTTGTCT GGTAAAANGA
  841 CANGGCCATC GCCAATTGGA NTATTTTGTT GATAATGGNC TGCTANTTGA ANNNANNCAT
  901 CTTCNNCGNN NNNGCGAANN NNGAANTTAN CTTTNANNCC ATTCTTTTGN TTGNCNGNCN
961 NGATGNATAC ATTNNGNNAN TNNAANNNNN NNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, GFP1-10 5')
Sequence file: RDB18568_B0J5c.seq check
>D07393A2_B0J5_1_TDH3pro_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NGNNNTGNNT GNANTTCTNN NNNCTATTTC TTAAACTTCT TAAATTCTAC
   61 TTTTATAGTT AGTCTTTTTT TTAGTTTTAA AACACCAGAA CTTAGTTTCG ACGGATGCGG
  121 CCGCTCTAGA ACTAGTGGAT CCGGAGGGTC TGGTGGCGGA TCAATGGGTG GCACTAGTAT
  181 GAGCAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA
  241 TGTTAATGGG CACAAATTTT CTGTCAGAGG AGAGGGTGAA GGTGATGCTA CAATCGGAAA
  301 ACTCACACTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACACTTGT
  361 CACTACTCTG ACCTATGGTG TTCAATGCTT TTCCCGTTAT CCGGATCACA TGAAAAGGCA
  421 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAACGCACTA TATCTTTCAA
  481 AGATGACGGG AAATACAAGA CGCGTGCTGT AGTCAAGTTT GAAGGTGATA CCCTTGTTAA
  541 TCGTATCGAG TTAAAGGGTA CTGATTTTAA AGAAGATGGA AACATTCTCG GACACAAACT
  601 CGAGTACAAC TTTAACTCAC ACAATGTATA CATCACGGCA GACAAACAAA AGAATGGAAT
  661 CAAAGCTAAC TTCACAGTTC GCCACAACGT TGAAGATGGT TCCGTTCAAC TAGCAGANCA
  721 TTATCAACAA AATACTCCNA TTGGCGATGG NCCTGTCCTT TTACCNGACA ACCATTACCT
  781 GTCGACACNA ACTGTCCTTT CGAAAGATCC CAACGAAAAG TAAGAATTCN ATATCNAGCT
  841 TATCGATACN GTCGANCTCN AGTCATGTAA TTAGTTATGT CACGCTNANN TTCNCGCCCN
  901 CCCCCCCNCA TNNGCNNTNN NCGAAAANGN ANGNANTTAG NNNNNCNNGN ANNNCTNNGN
  961 CCCNATTNAT TTTTTTNNNN NNNNNGNNNN NNNTNNNNNN NNTNNTNNNN NNTNNNANNT
1021 TTTNNNTTNN TTNTNNNNNN NNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB18568_B0J5d.seq check
>D07393A2_B0J5_1_TRP1pro_F_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCAGCTT GGNNTNNGTC TGTTATTAAT TTCNCAGGTA
   61 GTTCTGGTCC ATTGGTGAAA GTTTGCGGCT TGCAGAGCAC AGAGGCCGCA GAATGTGCTC
  121 TAGATTCCGA TGCTGACTTG CTGGGTATTA TATGTGTGCC CAATAGAAAG AGAACAATTG
  181 ACCCGGTTAT TGCAAGGAAA ATTTCAAGTC TTGTAAAAGC ATATAAAAAT AGTTCAGGCA
  241 CTCCGAAATA CTTGGTTGGC GTGTTTCGTA ATCAACCTAA GGAGGATGTT TTGGCTCTGG
  301 TCAATGATTA CGGCATTGAT ATCGTCCAAC TGCATGGAGA TGAGTCGTGG CAAGAATACC
  361 AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT TCCAAAAGAC TGCAACATAC
  421 TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC CTTGTTTGAT TCAGAAGCAG
  481 GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA CTGGGTTGGA AGGCAAGAGA
  541 GCCCCGAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG
  601 CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG
  661 TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG
  721 AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCGATC TATGCGGTGT GAAATACCGC
  781 ACAGATGCGT AANGAGAAAA TACCGCATCA NGAAATTGTA AGCGTTAATA TTTTGTTAAA
  841 ATTCGCGTTA AATTTTTGTN NAATCAGCTC ATTTTTTNNC CNATANGNCG AAATCGGNAA
  901 AATNCCTTAT AAATCAAAAG AATANANCGA NNTAGGGTTN ANNNNNGNTN CCNNTTTGGN
961 NNNNGANTNN NCTANTNANN NNNNNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, GFP1-10 3')
Sequence file: RDB18568_B0J5e.seq check
>D07393A2_B0J5_1_CYC1ter_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGG NNGNGGGGGG NNGGCGTGAA TGTAAGCGTG ACATAACTAA
   61 TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA CTTTTCGTTG
  121 GGATCTTTCG AAAGGACAGT TTGTGTCGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
  181 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGGA ACCATCTTCA
  241 ACGTTGTGGC GAACTGTGAA GTTAGCTTTG ATTCCATTCT TTTGTTTGTC TGCCGTGATG
  301 TATACATTGT GTGAGTTAAA GTTGTACTCG AGTTTGTGTC CGAGAATGTT TCCATCTTCT
  361 TTAAAATCAG TACCCTTTAA CTCGATACGA TTAACAAGGG TATCACCTTC AAACTTGACT
  421 ACAGCACGCG TCTTGTATTT CCCGTCATCT TTGAAAGATA TAGTGCGTTC CTGTACATAA
  481 CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCCTTTTCA TGTGATCCGG ANAACGGGAA
  541 AAGCNTTGAA CACCATANGT CANANTAGTG ACNAGTGNTG GNCNTGGAAC AGGTANTTTT
  601 CCNGTANNGN AAATAAATTT AAGTGNGANT TTTCCNANNG NANCATCNCC NNCNCCCTCT
661 NCNCNGANAN AAAANTTNNN NCNNNTNNNN TCNNCNTCTN NNNCNNNNNN NNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18568_B0J5f.seq check
>D07393A2_B0J5_1_Amp_R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNANTN NNCATTNATN NGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA
  181 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT
  241 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT
  301 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC
  361 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG
  421 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT
  481 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA
  541 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT
  601 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCTAAGAA ACCATTATTA
  661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
  721 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA NACGGTCACA GCTTGTCTGT
  781 AAGCGGANGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC
  841 GGGGCTGGNT TAACTATGCG GCATCAGANC ANANTGNACT GAGAGTGNAC CATANNTCAC
  901 GANNTTACTA TATATATAAT ATAGGAAGCA TTTNATAGAA CAGCATCNNA ATATATGTGT
  961 ACTTTGCANN TATGANGCCA GANGGNAGTA NNNGNAANAN ATTNNTNANN GNAAAANNAN
 1021 NNNGNNNNNN TNNCNNNNNN NNNTNNNNNN NGNNNNNTTN NNTTTTTNNN CNNNNNNNNN
1081 N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2020.11.06

GNP_filter3_RDBDEP_html_201104.pl