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pSFL9 pRS316-GPDp-GFP1-10

Expression vector of the first ten beta-strands (GFP1-10).

Catalog number RDB18567
Resource name pSFL9 pRS316-GPDp-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of the first ten beta-strands (GFP1-10), GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18567 pSFL9 pRS316-GPDp-GFP1-10 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18567_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB18567_B0J5a.seq check
>D07393A1_B0J5_2_HB-NOS_R_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GTANNGCCNG GGTTTTCCCN GTCACGACGT
   61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT
  121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT
  181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA
  241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT
  301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA
  361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT
  421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA
  481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGACCCACG CATGTATCTA
  541 TCTCATTTTC TTACACCTTC TATTACCTTC TGCTCTCTCT GATTTGGAAA AAGCTGAAAA
  601 AAAAGGTTGA AACCAGTTCC CTGAAATTAT TCCCCTACTT GACTAATAAG TATATAAAGA
  661 CGGTAGGTAT TGATTGTAAT TCTGTAAATC TATTTCTTAA ACTTCTTAAA TTCTACTTTT
  721 ATAGTTAGTC TTTTTTTTAG TTTTAAAACA CCAGAACTTA GTTTCGACGG ATGCGGCCGC
  781 TCTAGAACTA GTGGATCCGG ANGGTCTGGT GGCGGATCAA TGGGTGGCAC TAGTATGANC
  841 AAAGGAGAAG AACTTTTCNC TGGAGTTGTC CNNATTCTTG TTGAATTANA TGGTGATGTT
  901 NATGGGCACA AATTTTCTGT CANANNANAN GGTGNANNNN GANGCTACNN TCNNNAAAAC
961 TCACACTTAA ATTTATTTGC NNTANNNGNA AANTANCNNG NTNNNNNNNN NN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
Sequence file: RDB18567_B0J5b.seq check
>D07393A1_B0J5_2_SV40A3'ElementReverse_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCAA CGCGGCCTTT TTNCGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
  241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA
  301 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT
  361 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA
  421 ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC
  481 TGGTACCGGC CGCAAATTAA AGCCTTCGAG CGTCCCAAAA CCTTCTCAAG CAAGGTTTTC
  541 AGTATAATGT TACATGCGTA CACGCGTCTG TACAGAAAAA AAAGAAAAAT TTGAAATATA
  601 AATAACGTTC TTAATACTAA CATAACTATA AAAAAATAAA TAGGGACCTA GACTTCAGGT
  661 TGTCTAACTC CTTCCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT
  721 GACATAACTA ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT
  781 ACTTTTCGTT GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA
  841 AAAGGACAGG GCCATCGCCA ATTNGANTAN TTTGNTGANA ATGGNCTGCT ANTTGAANGG
  901 AANCNTCTTC NACGTNGTGG CGAACTGNNA ANTTAGCTTT GATTNCATTC TTTTGNTTGT
961 CTGNNNNNNN GTANACNTNN NGNGANTTAA AGNTNNNNNN NNNN
//
Primer: URA3pro_F
Region: URA3pro, URA3
Sequence file: RDB18567_B0J5c.seq check
>D07393A1_B0J5_2_URA3pro_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNN NNNNTGNNNT TGCCNNGTAT TCTTAACCCN ACTGCACAGA
   61 ACAAAAACCT GCAGGAAACG AAGATAAATC ATGTCGAAAG CTACATATAA GGAACGTGCT
  121 GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA TCATGCACGA AAAGCAAACA
  181 AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT TACTGGAGTT AGTTGAAGCA
  241 TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA TCTTGACTGA TTTTTCCATG
  301 GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT ACAATTTTTT ACTCTTCGAA
  361 GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC AGTACTCTGC GGGTGTATAC
  421 AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG TGGTGGGCCC AGGTATTGTT
  481 AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC CTAGAGGCCT TTTGATGTTA
  541 GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT ATACTAAGGG TACTGTTGAC
  601 ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG CTCAAAGAGA CATGGGTGGA
  661 AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG TGGGTTTAGA TGACAAGGGA
  721 GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG TCTCTACAGG ATCTGANATT
  781 ATTATTGTTG GAAGAGGANT ATTTGCAAAG GGAAGGGATG CTAANGTAGA GGGTGAACGT
  841 TACAGAAAAG CANGCTGGGA AGCATATTTG ANAAGANGCG GNCNGCAAAA CTNAAAAAAC
  901 TGTATTATAA GNNAATGNNT GTATACTAAA CTCNCNAATT ANAGNTTCNN TTTANTTATA
  961 TCAGTTATTA NCCTANNCGG NGNGAANNCC NCANAGANGC GTNANGNNAA AANNNNNNNN
1021 NCNNNAAANN GNANNCNNTN NNNNNTTTTN NNNNNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, GFP1-10)
Sequence file: RDB18567_B0J5d.seq check
>D07393A1_B0J5_2_TDH3pro_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNTGNNATT CTGTNNATCT ATTTCTTAAA CTTCTTAAAT
   61 TCTACTTTTA TAGTTAGTCT TTTTTTTAGT TTTAAAACAC CAGAACTTAG TTTCGACGGA
  121 TGCGGCCGCT CTAGAACTAG TGGATCCGGA GGGTCTGGTG GCGGATCAAT GGGTGGCACT
  181 AGTATGAGCA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT
  241 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGAGGAGAGG GTGAAGGTGA TGCTACAATC
  301 GGAAAACTCA CACTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGCCAACA
  361 CTTGTCACTA CTCTGACCTA TGGTGTTCAA TGCTTTTCCC GTTATCCGGA TCACATGAAA
  421 AGGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAACG CACTATATCT
  481 TTCAAAGATG ACGGGAAATA CAAGACGCGT GCTGTAGTCA AGTTTGAAGG TGATACCCTT
  541 GTTAATCGTA TCGAGTTAAA GGGTACTGAT TTTAAAGAAG ATGGAAACAT TCTCGGACAC
  601 AAACTCGAGT ACAACTTTAA CTCACACAAT GTATACATCA CGGCAGACAA ACAAAAGAAT
  661 GGAATCAAAG CTAACTTCAC AGTTCGCCAC AACGTTGAAG ATGGTTCCGT TCAACTAGCA
  721 GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT
  781 TACCTGTCGA CACAAACTGT CCTTTCGAAA GATCCCAACG AAAAGTAAGA ATTCGATATC
  841 AAGCTTATCG ATACCGTCNA CCTCGAGTCA TGTAATTAGT TATGTCACGC TTACATTCNC
  901 GCCCTCCCCC CNCATCCGCT CTNNCCGAAA AGGANGGAGT TAGANNACCN NAANNCTNNN
  961 TCCCNATTTA TTTTTTTATA GTTNTGTTAG TANNNNNANN GNNANTTNNA TTTCNAANTT
1021 TNNNTTTTTT TTNNNNNNNN NN
//
Primer: Amp_R
Region: AmpR, CEN_ARS
Sequence file: RDB18567_B0J5e.seq check
>D07393A1_B0J5_2_Amp_R_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGN NNCANTNATC NGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA
  181 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT
  241 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT
  301 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC
  361 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG
  421 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT
  481 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA
  541 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT
  601 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCTAAGAA ACCATTATTA
  661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
  721 GTGATGACGG TGAAAACCTC TGANACATGC AGCTCCCGGA NACGGTCACA GCTTGTCTGT
  781 AAGCGGATGC CGGGAGCAGA NAAGCCCGTC AGGGCGCGTC AGCGGGNGTT GGCGGGNGTC
  841 GGGGCTGGCT TAACTATGCG GCATCNNANC ANANTGNACT GAGAGTGNAC CNTACCACCT
  901 TTNNNATTNN TCATTTTTTT TTTTATTCNT TTTTTTTGAT TTCNGNTNNC TTGNAANNTT
961 TTTTTGANTC NNNAATCTNC NNNNNGANNN ANNAACNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.21

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