Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSFL9 pRS316-GPDp-GFP1-10

Expression vector of the first ten beta-strands (GFP1-10).

Catalog number RDB18567
Resource name pSFL9 pRS316-GPDp-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of the first ten beta-strands (GFP1-10), GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS316 (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB18567zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18567 pSFL9 pRS316-GPDp-GFP1-10 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pSFL9 pRS316-GPDp-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18567).

            Reference section:

            Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Visualizing multiple inter-organelle contact sites (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18567_B0J5p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: HB-NOS_R (Pr0800)
            Region: T7 pro, insert 5' (GPD pro)
            Sequence file: RDB18567_B0J5a.seq check
            >D07393A1_B0J5_2_HB-NOS_R_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GTANNGCCNG GGTTTTCCCN GTCACGACGT
               61 TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCAGT
              121 TTATCATTAT CAATACTCGC CATTTCAAAG AATACGTAAA TAATTAATAG TAGTGATTTT
              181 CCTAACTTTA TTTAGTCAAA AAATTAGCCT TTTAATTCTG CTGTAACCCG TACATGCCCA
              241 AAATAGGGGG CGGGTTACAC AGAATATATA ACATCGTAGG TGTCTGGGTG AACAGTTTAT
              301 TCCTGGCATC CACTAAATAT AATGGAGCCC GCTTTTTAAG CTGGCATCCA GAAAAAAAAA
              361 GAATCCCAGC ACCAAAATAT TGTTTTCTTC ACCAACCATC AGTTCATAGG TCCATTCTCT
              421 TAGCGCAACT ACAGAGAACA GGGGCACAAA CAGGCAAAAA ACGGGCACAA CCTCAATGGA
              481 GTGATGCAAC CTGCCTGGAG TAAATGATGA CACAAGGCAA TTGACCCACG CATGTATCTA
              541 TCTCATTTTC TTACACCTTC TATTACCTTC TGCTCTCTCT GATTTGGAAA AAGCTGAAAA
              601 AAAAGGTTGA AACCAGTTCC CTGAAATTAT TCCCCTACTT GACTAATAAG TATATAAAGA
              661 CGGTAGGTAT TGATTGTAAT TCTGTAAATC TATTTCTTAA ACTTCTTAAA TTCTACTTTT
              721 ATAGTTAGTC TTTTTTTTAG TTTTAAAACA CCAGAACTTA GTTTCGACGG ATGCGGCCGC
              781 TCTAGAACTA GTGGATCCGG ANGGTCTGGT GGCGGATCAA TGGGTGGCAC TAGTATGANC
              841 AAAGGAGAAG AACTTTTCNC TGGAGTTGTC CNNATTCTTG TTGAATTANA TGGTGATGTT
              901 NATGGGCACA AATTTTCTGT CANANNANAN GGTGNANNNN GANGCTACNN TCNNNAAAAC
            961 TCACACTTAA ATTTATTTGC NNTANNNGNA AANTANCNNG NTNNNNNNNN NN
            //
            Primer: SV40pA3'Element Reverse (Pr0108)
            Region: T3 pro, insert 3' (CYC1 ter, GFP1-10 3')
            Sequence file: RDB18567_B0J5b.seq check
            >D07393A1_B0J5_2_SV40A3'ElementReverse_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCAA CGCGGCCTTT TTNCGGTTCC
               61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
              121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
              181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG
              241 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA
              301 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT
              361 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA
              421 ACAGCTATGA CCATGATTAC GCCAAGCTCG AAATTAACCC TCACTAAAGG GAACAAAAGC
              481 TGGTACCGGC CGCAAATTAA AGCCTTCGAG CGTCCCAAAA CCTTCTCAAG CAAGGTTTTC
              541 AGTATAATGT TACATGCGTA CACGCGTCTG TACAGAAAAA AAAGAAAAAT TTGAAATATA
              601 AATAACGTTC TTAATACTAA CATAACTATA AAAAAATAAA TAGGGACCTA GACTTCAGGT
              661 TGTCTAACTC CTTCCTTTTC GGTTAGAGCG GATGTGGGGG GAGGGCGTGA ATGTAAGCGT
              721 GACATAACTA ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT
              781 ACTTTTCGTT GGGATCTTTC GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA
              841 AAAGGACAGG GCCATCGCCA ATTNGANTAN TTTGNTGANA ATGGNCTGCT ANTTGAANGG
              901 AANCNTCTTC NACGTNGTGG CGAACTGNNA ANTTAGCTTT GATTNCATTC TTTTGNTTGT
            961 CTGNNNNNNN GTANACNTNN NGNGANTTAA AGNTNNNNNN NNNN
            //
            Primer: URA3pro_F (Pr0170)
            Region: URA3pro, URA3
            Sequence file: RDB18567_B0J5c.seq check
            >D07393A1_B0J5_2_URA3pro_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNTNNNNN NNNNTGNNNT TGCCNNGTAT TCTTAACCCN ACTGCACAGA
               61 ACAAAAACCT GCAGGAAACG AAGATAAATC ATGTCGAAAG CTACATATAA GGAACGTGCT
              121 GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA TCATGCACGA AAAGCAAACA
              181 AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT TACTGGAGTT AGTTGAAGCA
              241 TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA TCTTGACTGA TTTTTCCATG
              301 GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT ACAATTTTTT ACTCTTCGAA
              361 GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC AGTACTCTGC GGGTGTATAC
              421 AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG TGGTGGGCCC AGGTATTGTT
              481 AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC CTAGAGGCCT TTTGATGTTA
              541 GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT ATACTAAGGG TACTGTTGAC
              601 ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG CTCAAAGAGA CATGGGTGGA
              661 AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG TGGGTTTAGA TGACAAGGGA
              721 GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG TCTCTACAGG ATCTGANATT
              781 ATTATTGTTG GAAGAGGANT ATTTGCAAAG GGAAGGGATG CTAANGTAGA GGGTGAACGT
              841 TACAGAAAAG CANGCTGGGA AGCATATTTG ANAAGANGCG GNCNGCAAAA CTNAAAAAAC
              901 TGTATTATAA GNNAATGNNT GTATACTAAA CTCNCNAATT ANAGNTTCNN TTTANTTATA
              961 TCAGTTATTA NCCTANNCGG NGNGAANNCC NCANAGANGC GTNANGNNAA AANNNNNNNN
            1021 NCNNNAAANN GNANNCNNTN NNNNNTTTTN NNNNNNNNN
            //
            Primer: TDH3pro_F (Pr0431)
            Region: insert mid (GPD pro, GFP1-10)
            Sequence file: RDB18567_B0J5d.seq check
            >
            //
            Primer: Amp_R (Pr0008)
            Region: AmpR, CEN_ARS
            Sequence file: RDB18567_B0J5e.seq check
            >D07393A1_B0J5_2_Amp_R_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTGN NNCANTNATC NGGGTTATTG TCTCATGAGC GGATACATAT
               61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
              121 CACCTGGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA
              181 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT
              241 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT
              301 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC
              361 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG
              421 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT
              481 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA
              541 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT
              601 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCTAAGAA ACCATTATTA
              661 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG
              721 GTGATGACGG TGAAAACCTC TGANACATGC AGCTCCCGGA NACGGTCACA GCTTGTCTGT
              781 AAGCGGATGC CGGGAGCAGA NAAGCCCGTC AGGGCGCGTC AGCGGGNGTT GGCGGGNGTC
              841 GGGGCTGGCT TAACTATGCG GCATCNNANC ANANTGNACT GAGAGTGNAC CNTACCACCT
              901 TTNNNATTNN TCATTTTTTT TTTTATTCNT TTTTTTTGAT TTCNGNTNNC TTGNAANNTT
            961 TTTTTGANTC NNNAATCTNC NNNNNGANNN ANNAACNANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl