Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.
Alternative name | px330-PGK-puro-pA |
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Clone info. | Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance. |
Vector backbone | pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid) |
Size of vector backbone | 8.5 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse PGK genomic DNA |
Depositor|Developer | Yamada, Yasuhiro | |
Sequence (full) ![]() |
RDB18386zzk02.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019) |
Remarks | The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word] ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)。 |
備考 | 本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18386 | px330-PPP | DNA solution |
Materials & Methods section:
The px330-PPP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18386). |
Reference section:
Sakakura, M., Ohta, S., Yagi, M., Tanaka, A., Norihide, J., Woltjen, K., Yamamoto, T., Yamada, Y. , Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18386_B0Hop1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: PuroR-rev (Pr0423) Region: insert 5', bGH pA Sequence file: RDB18386_B0Hoa.seq ![]() |
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>D07192A1_B0Ho_2_PuroR-rev_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNA NNATACTATG CCGATATACT ATGCCGATGA TTAATTGTCA 61 ACAGGCTGCA GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC 121 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC 181 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA GCCCAGAAAG 241 CGAAGGAGCA AAGCTGCTAT TGGCCGCTGC CCCAAAGGCC TACCCGCTTC CATTGCTCAG 301 CGGTGCTGTC CATCTGCACG AGACTAGTGA GACGTGCTAC TTCCATTTGT CACGTCCTGC 361 ACGACGCGAG CTGCGGGGCG GGGGGGAACT TCCTGACTAG GGGAGGAGTA GAAGGTGGCG 421 CGAAGGGGCC ACCAAAGAAC GGAGCCGGTT GGCGCCTACC GGTGGATGTG GAATGTGTGC 481 GAGGCCAGAG GCCACTTGTG TAGCGCCAAG TGCCCAGCGG GGCTGCTAAA GCGCATGCTC 541 CAGACTGCCT TGGGAAAAGC GCCTCCCCTA CCCGGTAGAG CGGCCGCTCC CCAGCATGCC 601 TGCTATTCTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG 661 AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG 721 GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGANGG GCAAACAACA GATGGCTGGC 781 AACTAGAANG CACAGTCGAG GCTGATCAGC GAGCTCTANG AATTCTTACT TTTTCTTTTT 841 TGCCTGGCCG GNCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCNNNCT GANACAGGTC 901 GATCNGTGTC TCGTACAGGN CGGNGNNGCT CTNGNNNNTC ANGGTGGCGT CCAGCANCTC 961 TTTGGNGCTG GNGTACNNCT TCCNNNCGAN GGNNGNGNCA AANNANTTGN ANNNNNNNNG 1021 GGNNNCCNNN NNNNNNN // |
Primer: PGKpro_F (Pr0249) Region: insert 3' Sequence file: RDB18386_B0Hob.seq ![]() |
> // |
Primer: U6pro_F3 (Pr0648) Region: hU6 pro, gRNA scafforld, CBh pro Sequence file: RDB18386_B0Hoc.seq ![]() |
>D07192A1_B0Ho_2_U6pro_F3_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNANNGGN NNATNNTATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC 61 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGGT CTTCGAGAAG ACCTGTTTTA 121 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC 181 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT 241 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA 301 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA 361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT 421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA 481 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT 541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA 601 CTCTCCCCAT CTCCCCCCCC TCCCCACCCC CAATTTTGTA TTTATTTATT TTTTAATTAT 661 TTTGTGCAGC GATGGGGGCG GGGGGGGGGG GGGGNNNNNN NNNN // |
Primer: CAG_R2 (Pr0585) Region: CBh pro, 3xFLAG, SV40 NSL, Cas9 5' Sequence file: RDB18386_B0Hod.seq ![]() |
>D07192A1_B0Ho_2_CAG_R2_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCNN NGCNCCGCCG CCGCCTCGCG 61 CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC 121 TTCTCCTCCG GGCTGTAATT AGCTGAGCAA GAGGTAAGGG TTTAAGGGAT GGTTGGTTGG 181 TGGGGTATTA ATGTTTAATT ACCTGGAGCA CCTGCCTGAA ATCACTTTTT TTCAGGTTGG 241 ACCGGTGCCA CCATGGACTA TAAGGACCAC GACGGAGACT ACAAGGATCA TGATATTGAT 301 TACAAAGACG ATGACGATAA GATGGCCCCA AAGAAGAAGC GGAAGGTCGG TATCCACGGA 361 GTCCCAGCAG CCGACAAGAA GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC 421 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC 481 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC TGCTGTTCGA CAGCGGCGAA 541 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC 601 CGGATCTGCT ATCTGCAAGA GATCTTCNGC AACGAGATGG CCAAGGTGGA CGACAGCTTC 661 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC 721 ATCTTCGGCA ACATCGTGGA CGANGTGGCC TANNNNNNGA AGTACCCCAC CATCTACCAC 781 CTGANAAAGA NACTGGTGGA CAGCNCCGAN NANNGNCGAN CTGCGGCTGA TCTATCTGGN 841 CCNGGCCCAC ATGATCAAGT TCCGGGGCNN CTTNCTGATC NAGGGCGANN TGANNCCCCG 901 ACNANAGCGA CGTGNANNAG CTGNTNNNCN AGCNGGNNNA NANCNNNNAN NNNNNNNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA, Cas9 3' Sequence file: RDB18386_B0Hoe.seq ![]() |
>D07192A1_B0Ho_2_bGH_rev3_F03_18_ABI24.ab1 1 NNNNNNNNNN NGNNNGGNNG NNGGNNNNNN ACAGANGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT 121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA 181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC 241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA 301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT 361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT 421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT 481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA 541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG 601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA 661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT 721 CGAAGCTGCT TCTTTCCATG ATGGTGNTCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT 781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATANGCCACG GTGGGGCTGT 841 CGAAGCCGCC GTACTTCTTA NGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT 901 NNCTCTTGGG CAGGANAGAC TCTTTGCTGA AGCCGCCNGT CTGCACCNCG GNCTTTTTCN 961 CGATATTCAC TTNNNNNNGN NCANNANNTN NCNGCANGGN GGNNAANNCN NGNNCNNTNN 1021 NNCNNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Sakakura, M., Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [link to RRC of NBRP] |
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