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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

px330-PPP (#RDB18386)

Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.

Alternative name px330-PGK-puro-pA
Clone info. Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name mouse PGK genomic DNA
Depositor|Developer Yamada, Yasuhiro |
 
Sequence (full) RDB18386zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18386 px330-PPP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The px330-PPP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18386).

Reference section:

Sakakura, M., Ohta, S., Yagi, M., Tanaka, A., Norihide, J., Woltjen, K., Yamamoto, T., Yamada, Y. , Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18386_B0Hop1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: PuroR-rev (Pr0423)
Region: insert 5', bGH pA
Sequence file: RDB18386_B0Hoa.seq check
>D07192A1_B0Ho_2_PuroR-rev_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNA NNATACTATG CCGATATACT ATGCCGATGA TTAATTGTCA
   61 ACAGGCTGCA GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC
  121 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC
  181 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA GCCCAGAAAG
  241 CGAAGGAGCA AAGCTGCTAT TGGCCGCTGC CCCAAAGGCC TACCCGCTTC CATTGCTCAG
  301 CGGTGCTGTC CATCTGCACG AGACTAGTGA GACGTGCTAC TTCCATTTGT CACGTCCTGC
  361 ACGACGCGAG CTGCGGGGCG GGGGGGAACT TCCTGACTAG GGGAGGAGTA GAAGGTGGCG
  421 CGAAGGGGCC ACCAAAGAAC GGAGCCGGTT GGCGCCTACC GGTGGATGTG GAATGTGTGC
  481 GAGGCCAGAG GCCACTTGTG TAGCGCCAAG TGCCCAGCGG GGCTGCTAAA GCGCATGCTC
  541 CAGACTGCCT TGGGAAAAGC GCCTCCCCTA CCCGGTAGAG CGGCCGCTCC CCAGCATGCC
  601 TGCTATTCTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG
  661 AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG
  721 GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGANGG GCAAACAACA GATGGCTGGC
  781 AACTAGAANG CACAGTCGAG GCTGATCAGC GAGCTCTANG AATTCTTACT TTTTCTTTTT
  841 TGCCTGGCCG GNCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCNNNCT GANACAGGTC
  901 GATCNGTGTC TCGTACAGGN CGGNGNNGCT CTNGNNNNTC ANGGTGGCGT CCAGCANCTC
  961 TTTGGNGCTG GNGTACNNCT TCCNNNCGAN GGNNGNGNCA AANNANTTGN ANNNNNNNNG
1021 GGNNNCCNNN NNNNNNN
//
Primer: PGKpro_F (Pr0249)
Region: insert 3'
Sequence file: RDB18386_B0Hob.seq check
>
//
Primer: U6pro_F3 (Pr0648)
Region: hU6 pro, gRNA scafforld, CBh pro
Sequence file: RDB18386_B0Hoc.seq check
>D07192A1_B0Ho_2_U6pro_F3_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNANNGGN NNATNNTATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC
   61 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGGT CTTCGAGAAG ACCTGTTTTA
  121 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC
  181 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT
  241 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA
  301 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA
  361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA
  481 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT
  541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA
  601 CTCTCCCCAT CTCCCCCCCC TCCCCACCCC CAATTTTGTA TTTATTTATT TTTTAATTAT
661 TTTGTGCAGC GATGGGGGCG GGGGGGGGGG GGGGNNNNNN NNNN
//
Primer: CAG_R2 (Pr0585)
Region: CBh pro, 3xFLAG, SV40 NSL, Cas9 5'
Sequence file: RDB18386_B0Hod.seq check
>D07192A1_B0Ho_2_CAG_R2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCNN NGCNCCGCCG CCGCCTCGCG
   61 CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC
  121 TTCTCCTCCG GGCTGTAATT AGCTGAGCAA GAGGTAAGGG TTTAAGGGAT GGTTGGTTGG
  181 TGGGGTATTA ATGTTTAATT ACCTGGAGCA CCTGCCTGAA ATCACTTTTT TTCAGGTTGG
  241 ACCGGTGCCA CCATGGACTA TAAGGACCAC GACGGAGACT ACAAGGATCA TGATATTGAT
  301 TACAAAGACG ATGACGATAA GATGGCCCCA AAGAAGAAGC GGAAGGTCGG TATCCACGGA
  361 GTCCCAGCAG CCGACAAGAA GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC
  421 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC
  481 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC TGCTGTTCGA CAGCGGCGAA
  541 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC
  601 CGGATCTGCT ATCTGCAAGA GATCTTCNGC AACGAGATGG CCAAGGTGGA CGACAGCTTC
  661 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC
  721 ATCTTCGGCA ACATCGTGGA CGANGTGGCC TANNNNNNGA AGTACCCCAC CATCTACCAC
  781 CTGANAAAGA NACTGGTGGA CAGCNCCGAN NANNGNCGAN CTGCGGCTGA TCTATCTGGN
  841 CCNGGCCCAC ATGATCAAGT TCCGGGGCNN CTTNCTGATC NAGGGCGANN TGANNCCCCG
901 ACNANAGCGA CGTGNANNAG CTGNTNNNCN AGCNGGNNNA NANCNNNNAN NNNNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, Cas9 3'
Sequence file: RDB18386_B0Hoe.seq check
>D07192A1_B0Ho_2_bGH_rev3_F03_18_ABI24.ab1
    1 NNNNNNNNNN NGNNNGGNNG NNGGNNNNNN ACAGANGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT
  121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA
  181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC
  241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA
  301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT
  361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT
  421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT
  481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA
  541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG
  601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA
  661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT
  721 CGAAGCTGCT TCTTTCCATG ATGGTGNTCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT
  781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATANGCCACG GTGGGGCTGT
  841 CGAAGCCGCC GTACTTCTTA NGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT
  901 NNCTCTTGGG CAGGANAGAC TCTTTGCTGA AGCCGCCNGT CTGCACCNCG GNCTTTTTCN
  961 CGATATTCAC TTNNNNNNGN NCANNANNTN NCNGCANGGN GGNNAANNCN NGNNCNNTNN
1021 NNCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sakakura, M., Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [link to RRC of NBRP]