Please review the QC test results indicated by icon below as well as clone information before placing your order.

px330-PPP

Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.

Sequence (full)	RDB18386zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)。
提供依頼	提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18386_B0Hop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: PuroR-rev (Pr0423) Region: insert 5', bGH pA Sequence file: RDB18386_B0Hoa.seq
>D07192A1_B0Ho_2_PuroR-rev_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNA NNATACTATG CCGATATACT ATGCCGATGA TTAATTGTCA    61 ACAGGCTGCA GGTCGAAAGG CCCGGAGATG AGGAAGAGGA GAACAGCGCG GCAGACGTGC   121 GCTTTTGAAG CGTGCAGAAT GCCGGGCCTC CGGAGGACCT TCGGGCGCCC GCCCCGCCCC   181 TGAGCCCGCC CCTGAGCCCG CCCCCGGACC CACCCCTTCC CAGCCTCTGA GCCCAGAAAG   241 CGAAGGAGCA AAGCTGCTAT TGGCCGCTGC CCCAAAGGCC TACCCGCTTC CATTGCTCAG   301 CGGTGCTGTC CATCTGCACG AGACTAGTGA GACGTGCTAC TTCCATTTGT CACGTCCTGC   361 ACGACGCGAG CTGCGGGGCG GGGGGGAACT TCCTGACTAG GGGAGGAGTA GAAGGTGGCG   421 CGAAGGGGCC ACCAAAGAAC GGAGCCGGTT GGCGCCTACC GGTGGATGTG GAATGTGTGC   481 GAGGCCAGAG GCCACTTGTG TAGCGCCAAG TGCCCAGCGG GGCTGCTAAA GCGCATGCTC   541 CAGACTGCCT TGGGAAAAGC GCCTCCCCTA CCCGGTAGAG CGGCCGCTCC CCAGCATGCC   601 TGCTATTCTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG   661 AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG   721 GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGANGG GCAAACAACA GATGGCTGGC   781 AACTAGAANG CACAGTCGAG GCTGATCAGC GAGCTCTANG AATTCTTACT TTTTCTTTTT   841 TGCCTGGCCG GNCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCNNNCT GANACAGGTC   901 GATCNGTGTC TCGTACAGGN CGGNGNNGCT CTNGNNNNTC ANGGTGGCGT CCAGCANCTC   961 TTTGGNGCTG GNGTACNNCT TCCNNNCGAN GGNNGNGNCA AANNANTTGN ANNNNNNNNG 1021 GGNNNCCNNN NNNNNNN //
Primer: PGKpro_F (Pr0249) Region: insert 3' Sequence file: RDB18386_B0Hob.seq
>D07192A1_B0Ho_2_PGKpro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNTCTCNGG GCCTTTCGAC CTGCAGCCTG TTGACNATTA    61 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGAC   121 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC   181 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA   241 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG   301 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT   361 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG   421 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC   481 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC   541 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC   601 CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT   661 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGATGACA   721 CTCGACCTCG AAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA   781 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA   841 TCAATGTATC TTATCATGTC TGCCTGANGC GGTATTTTCT CCTTACGCAT CTGTGCGGNA   901 TTTCACACCG CANACGTCNA AGCAANNATA GTANGCGCCC NGTANCGGCG CANTAANCGC   961 GGCGGGNGTG GNGGTTACGC GCANCGNGNC CGNNACNNNN GCCAGCGCCN TANCGNCNNN 1021 NNCNTTTNGN NTTNNNNCNN TNNNNTNNNN NNNNNN //
Primer: U6pro_F3 (Pr0648) Region: hU6 pro, gRNA scafforld, CBh pro Sequence file: RDB18386_B0Hoc.seq
>D07192A1_B0Ho_2_U6pro_F3_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNANNGGN NNATNNTATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC    61 TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGGT CTTCGAGAAG ACCTGTTTTA   121 GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC   181 GAGTCGGTGC TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT   241 TTTAGCGCGT GCGCCAATTC TGCAGACAAA TGGCTCTAGA GGTACCCGTT ACATAACTTA   301 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAGTAA   361 CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT   421 TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA   481 AATGGCCCGC CTGGCATTGT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT   541 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT GAGCCCCACG TTCTGCTTCA   601 CTCTCCCCAT CTCCCCCCCC TCCCCACCCC CAATTTTGTA TTTATTTATT TTTTAATTAT 661 TTTGTGCAGC GATGGGGGCG GGGGGGGGGG GGGGNNNNNN NNNN //
Primer: CAG_R2 (Pr0585) Region: CBh pro, 3xFLAG, SV40 NSL, Cas9 5' Sequence file: RDB18386_B0Hod.seq
>D07192A1_B0Ho_2_CAG_R2_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCNNGCNN NGCNCCGCCG CCGCCTCGCG    61 CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC   121 TTCTCCTCCG GGCTGTAATT AGCTGAGCAA GAGGTAAGGG TTTAAGGGAT GGTTGGTTGG   181 TGGGGTATTA ATGTTTAATT ACCTGGAGCA CCTGCCTGAA ATCACTTTTT TTCAGGTTGG   241 ACCGGTGCCA CCATGGACTA TAAGGACCAC GACGGAGACT ACAAGGATCA TGATATTGAT   301 TACAAAGACG ATGACGATAA GATGGCCCCA AAGAAGAAGC GGAAGGTCGG TATCCACGGA   361 GTCCCAGCAG CCGACAAGAA GTACAGCATC GGCCTGGACA TCGGCACCAA CTCTGTGGGC   421 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC   481 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC TGCTGTTCGA CAGCGGCGAA   541 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC   601 CGGATCTGCT ATCTGCAAGA GATCTTCNGC AACGAGATGG CCAAGGTGGA CGACAGCTTC   661 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC   721 ATCTTCGGCA ACATCGTGGA CGANGTGGCC TANNNNNNGA AGTACCCCAC CATCTACCAC   781 CTGANAAAGA NACTGGTGGA CAGCNCCGAN NANNGNCGAN CTGCGGCTGA TCTATCTGGN   841 CCNGGCCCAC ATGATCAAGT TCCGGGGCNN CTTNCTGATC NAGGGCGANN TGANNCCCCG 901 ACNANAGCGA CGTGNANNAG CTGNTNNNCN AGCNGGNNNA NANCNNNNAN NNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, Cas9 3' Sequence file: RDB18386_B0Hoe.seq
>D07192A1_B0Ho_2_bGH_rev3_F03_18_ABI24.ab1     1 NNNNNNNNNN NGNNNGGNNG NNGGNNNNNN ACAGANGGCT GGCAACTAGA AGGCACAGTC    61 GAGGCTGATC AGCGAGCTCT AGGAATTCTT ACTTTTTCTT TTTTGCCTGG CCGGCCTTTT   121 TCGTGGCCGC CGGCCTTTTG TCGCCTCCCA GCTGAGACAG GTCGATCCGT GTCTCGTACA   181 GGCCGGTGAT GCTCTGGTGG ATCAGGGTGG CGTCCAGCAC CTCTTTGGTG CTGGTGTACC   241 TCTTCCGGTC GATGGTGGTG TCAAAGTACT TGAAGGCGGC AGGGGCTCCC AGATTGGTCA   301 GGGTAAACAG GTGGATGATA TTCTCGGCCT GCTCTCTGAT GGGCTTATCC CGGTGCTTGT   361 TGTAGGCGGA CAGCACTTTG TCCAGATTAG CGTCGGCCAG GATCACTCTC TTGGAGAACT   421 CGCTGATCTG CTCGATGATC TCGTCCAGGT AGTGCTTGTG CTGTTCCACA AACAGCTGTT   481 TCTGCTCATT ATCCTCGGGG GAGCCCTTCA GCTTCTCATA GTGGCTGGCC AGGTACAGGA   541 AGTTCACATA TTTGGAGGGC AGGGCCAGTT CGTTTCCCTT CTGCAGTTCG CCGGCAGAGG   601 CCAGCATTCT CTTCCGGCCG TTTTCCAGCT CGAACAGGGA GTACTTAGGC AGCTTGATGA   661 TCAGGTCCTT TTTCACTTCT TTGTAGCCCT TGGCTTCCAG AAAGTCGATG GGATTCTTCT   721 CGAAGCTGCT TCTTTCCATG ATGGTGNTCC CCAGCAGCTC TTTCACACTC TTCAGTTTCT   781 TGGACTTGCC CTTTTCCACT TTGGCCACCA CCAGCACAGA ATANGCCACG GTGGGGCTGT   841 CGAAGCCGCC GTACTTCTTA NGGTCCCAGT CCTTCTTTCT GGCGATCAGC TTATCGCTGT   901 NNCTCTTGGG CAGGANAGAC TCTTTGCTGA AGCCGCCNGT CTGCACCNCG GNCTTTTTCN   961 CGATATTCAC TTNNNNNNGN NCANNANNTN NCNGCANGGN GGNNAANNCN NGNNCNNTNN 1021 NNCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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