RDB18386 - px330-PPP

px330-PPP (#RDB18386)

Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Sakakura, M., Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Alternative name	px330-PGK-puro-pA
Clone info.	Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.
Vector backbone	pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone	8.5 kb
Selectable markers	Ampicillin
Growth conditions	LB+Amp, 37oC
Gene/insert name	mouse PGK genomic DNA
Depositor\|Developer	Yamada, Yasuhiro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee
RDB18386	px330-PPP	DNA solution

How to cite this biological resource

Materials & Methods section:

The px330-PPP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18386).

Reference section:

Sakakura, M., Ohta, S., Yagi, M., Tanaka, A., Norihide, J., Woltjen, K., Yamamoto, T., Yamada, Y. , Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Sakakura, M., Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342. [PubMed] [Article] [RRC of NBRP]

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External Database
mouse PGK

Reference sequence