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px330-PPP

Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.

Catalog number RDB18386
Resource name px330-PPP
Alternative name px330-PGK-puro-pA
Clone info. Expression vector of human codon-optimized SpCas9 and gRNA, puromycin resistance.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Ampicillin
Gene/insert name mouse PGK genomic DNA
Depositor|Developer Yamada, Yasuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sakakura, M. et. Al., Biochem. Biophys. Res. Commun. 519(4): 705-713, 2019)
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18386 px330-PPP Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18386zzk01.seq

Original reference

original Sakakura, M., Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Sakakura, M., Smarcb1 maintains the cellular identity and the chromatin landscapes of mouse embryonic stem cells. Biochem. Biophys. Res. Commun. 519 (4): 705-713 (2019). PMID 31543342.

2021.03.11

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