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pET30c-streptag-TET2

Bacterial expression vector encoding the catalytic domain of human TET2.

Catalog number RDB18377
Resource name pET30c-streptag-TET2
Clone info. Bacterial expression vector of the codon-optimized catalytic domain of human Ten-eleven translocation 2 (TET2), composed of a Streptag at the N-terminal, amino acids corresponding to 1129-1480th aa and 1844-1936th aa of NM_001127208.3 linked with a 15-residue GS linker (GGGGSGGGGSGGGGS). See Fig.S1 of Taka, N. and Yoshida, W., Anal. Bioanal. Chem., 412 (22): 5299-5305, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE.
Vector backbone pET30c (plasmid)
Size of vector backbone 6.7 kb
Selectable markers Kanamycin
Gene/insert name human TET2 cDNA
Depositor|Developer Yoshida, Wataru |
Other clones in our bank
External Database
human TET2
          Reference sequence
            
           
          Sequence (full) RDB18377zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Taka, N. and Yoshida, W. Anal. Bioanal. Chem. 412(22): 5299-5305, 2020)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Taka, N. and Yoshida, W. Anal. Bioanal. Chem. 412(22): 5299-5305, 2020)。
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          RDB18377 pET30c-streptag-TET2 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Taka, N., Quantification of global DNA methylation level using 5-methylcytosine dioxygenase. Anal. Bioanal. Chem. 412 (22): 5299-5305 (2020). PMID 32504107.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Taka, N., Quantification of global DNA methylation level using 5-methylcytosine dioxygenase. Anal. Bioanal. Chem. 412 (22): 5299-5305 (2020). PMID 32504107.
          protocol Taka, N., Global DNA Methylation Analysis Using Methylcytosine Dioxygenase. In: Yuan BF. (eds) DNA Modification Detection Methods. Springer Protocols Handbooks. Humana, New York, NY. pp 93-102 (2021).

          2022.08.12

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