Resource data sheet
DNA Bank Top

pUC18C_mPAG2 (#RDB18374)

Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.


Drawn by SnapGene® software
Sequence (full) RDB18374hts01.seq
Publication Unpublished bioresource
Test sheet RDB18374_B1Gcp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Chloramphenicol resistance. The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Chloramphenicol, pheS
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18374 pUC18C_mPAG2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC18C_mPAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18374).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP]