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Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.
| Catalog number | RDB18374 |
|---|---|
| Resource name | pUC18C_mPAG2 |
| Clone info. | Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Chloramphenicol resistance. The risk of recombination is lower. Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine. |
| Vector backbone | pUC18 (plasmid) |
| Selectable markers | Chloramphenicol, pheS |
| Gene/insert name | Escherichia coli pheS genomic DNA |
| Depositor|Developer | Suzuki, Kaoru | Miyazaki, Kentaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) |
|---|---|
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
|---|---|---|---|---|
| RDB18374 | pUC18C_mPAG2 | Under QC test. Please contact us. | DNA solution |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.Electronic file
| Electronic file | Nucleotide sequence provided by the depositor RDB18374zzk01.seq |
|---|
Original reference
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
2021.01.06
GNP_filter3_RDBDEP_html_201222.pl