Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC18C_mPAG2

Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.

Sequence (full)	RDB18374zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18374_B0H5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pKD3verN3 Region: cat 3', mPAG2 5' Sequence file: RDB18374_B0H5a.seq
>D07025A3_B0H5_1_pKD3verN3_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTNN NTGAGANNNN GTTTTTCGTC TCNGCCNATC CCTGGGTGAG    61 TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC   121 CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA   181 TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG   241 CGATGAGTGG CAGGGCGGGG CGTAAAGGAG GTAAAACACA TGTCACACCT AGCAGAATTA   301 GTTGCTAGTG CAAAAGCTGC AATATCACAA GCTAGCGATG TTGCGGCGCT GGACAATGTG   361 CGTGTGGAGT ACCTGGGTAA GAAAGGCCAC CTGACCCTGC AGATGACCAC CCTGCGTGAA   421 CTGCCGCCGG AGGAACGTCC GGCGGCGGGT GCGGTTATCA ACGAGGCGAA GGAACAAGTG   481 CAGCAAGCGC TGAACGCGCG TAAAGCGGAG CTGGAAAGCG CGGCGCTGAA CGCGCGTCTG   541 GCGGCGGAGA CCATTGACGT TAGCCTGCCG GGTCGTCGTA TTGAAAACGG TGGCCTGCAC   601 CCGGTGACCC GTACCATCGA TCGTATTGAG AGCTTCTTTG GTGAACTGGG CTTCACCGTT   661 GCGACCGGTC CGGAGATCGA AGACGATTAT CACAACTTTG ATGCGCTGAA CATTCCGGGT   721 CACCACCCGG CGCGTGCGGA CCACGATACC TTCTGGTTTG ATACNACCCG TCTGCTGCGT   781 ACCCAGANCA GCGGTGTTCN AATCCGTACC ATGAAGGCGC AGCAACCGCC GANTCGTATC   841 ATTGCGCCGG GCCGTGTGTA CCGNNNCGAC TATGATCAGA NCCACACCCN NATGTTCCNN   901 CNAATGGANG GNCTGNTCGN GGNNNCCNAC NTTANCTTCN NNNACCNGAA AGGNANCCTG   961 NNCNANTTNN TGCNTNNCNN NNTTGANNNN NNNNNGNNNA NTNNNTTNNN NNNNNANNNN 1021 NNTNNCNNNT TNNNNNNNNN N //
Primer: ePAG_R1 Region: mPAG2 3' Sequence file: RDB18374_B0H5b.seq
>D07025A3_B0H5_1_ePAG_R1_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNANNN GNNCTTNNCC TAGNNCCTTT TAAATTAAAA ATGAAGTTTT    61 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GGAATTATTA CTTAAATTGT   121 TTCAGGAAGC GCAGATCGTT TTCAAAGAAG CTACGCAGGT CGGTCACGCC ATAACGCAGC   181 ATGGTCAGAC GTTCCATGCC CATACCAAAG CCGAAACCGC TGTACACTTC CGGATCAATA   241 CCCACGTTAC GCAGAACGTT CGGGTGCACC ATGCCGCAAC CCAGAACTTC CAGCCATTTG   301 CCGTTCTTGC CCATAACGTC CACTTCCGCG CTCGGCTCCG CAAACGGGAA GTAGCTCGGA   361 CGAAAACGAA TCTGCAGATC TTCCTCAAAG AAGTTACGCA GAAAGTCGTG CAGGGTGCCT   421 TTCAGGTTGG TGAAGCTAAT GTTGGTGTCC ACGATCAGAC CCTCCATTTG GTGGAACATC   481 GGGGTGTGGG TCTGATCATA GTCGTTACGG TACACACGGC CCGGCGCAAT GATACGAATC   541 GGCGGTTGCT GCGCCTTCAT GGTACGGATT TGAACACCGC TGGTCTGGGT ACGCAGCAGA   601 CGGGTGGTAT CAAACCAGAA GGTATCGTGG TCCGCACGCG CCGGGTGGTG ACCCGGAATG   661 TTCAGCGCAT CAAAGTTGTG ATAATCGTCT TCGATCTCCG GACCGGTCGC AACGGTGAAG   721 CCCAGTTCAC CAAAGAAGCT CTCAATACGA TCGATGGTAC GGGTCACCGG GTGCAGGCCA   781 CCGTTTTCNA TACGACGANC CGGCANGCTA ACGTCAATGG TCTCCGCCGC CAGANGCGCG   841 TTCAGCGCCG CGCTTTCCAG CTCCGCTTNA CGCGCGTTCA GCGCTNGCTG CACTTGNNCC   901 TTCNNNNCGT TGANAANCGC ACCCGCCGCN NGANGTTCCT CCGGCNNNNN TTCACGCAGG   961 GNGGNCATCT GCNNGGNCNN NTGNNNTTTN NNANCCNNGN NNTNNNNNNG CACANTGTCN 1021 NNCNNNNNNN NNN //
Primer: pGEX3' Region: AmpR pro, cat Sequence file: RDB18374_B0H5c.seq
>D07025A3_B0H5_1_pGEX3'_F09_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGCCTCG TGANACGCCT ATTTTTATAG GTTAATGTCA    61 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC   121 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT   181 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGGA GAAAAAAATC ACTGGATATA   241 CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG   301 CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA   361 AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG   421 CTCATCCGGA ATTTCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC   481 ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT   541 ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG   601 AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC   661 CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCNA TATGGACAAC TTCTTCGCCC   721 CCGTTTTCNC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA   781 TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGANTGCTT AATGAATTAC   841 ANNNNTACTG CNATGANTGG CNNGNCNGGG CGTAAAGGAN GTNAAACNCA TGTCACANCT   901 ANCANAANNN NNTTGCTAGT GCNAAAGCTG NNATATCNCN ANGCTAGCGA TGTTGCNGCN   961 CTNGNNNNAT GNGCGTGTGG NNTANNTGGG NNANAANNNN NNNCTNNNCN NNNNNANGAN 1021 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl