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pUC18C_mPAG2

Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance.

Catalog number RDB18374
Resource name pUC18C_mPAG2
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Chloramphenicol resistance. The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Chloramphenicol, pheS
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |
Other clones in our bank

External Database
Escherichia coli pheS

          Reference sequence
            
           
          Sequence (full) RDB18374zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。
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          RDB18374 pUC18C_mPAG2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18374_B0H5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pKD3verN3
          Region: cat 3', mPAG2 5'
          Sequence file: RDB18374_B0H5a.seq check
          >D07025A3_B0H5_1_pKD3verN3_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNTNN NTGAGANNNN GTTTTTCGTC TCNGCCNATC CCTGGGTGAG
             61 TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC
            121 CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA
            181 TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG
            241 CGATGAGTGG CAGGGCGGGG CGTAAAGGAG GTAAAACACA TGTCACACCT AGCAGAATTA
            301 GTTGCTAGTG CAAAAGCTGC AATATCACAA GCTAGCGATG TTGCGGCGCT GGACAATGTG
            361 CGTGTGGAGT ACCTGGGTAA GAAAGGCCAC CTGACCCTGC AGATGACCAC CCTGCGTGAA
            421 CTGCCGCCGG AGGAACGTCC GGCGGCGGGT GCGGTTATCA ACGAGGCGAA GGAACAAGTG
            481 CAGCAAGCGC TGAACGCGCG TAAAGCGGAG CTGGAAAGCG CGGCGCTGAA CGCGCGTCTG
            541 GCGGCGGAGA CCATTGACGT TAGCCTGCCG GGTCGTCGTA TTGAAAACGG TGGCCTGCAC
            601 CCGGTGACCC GTACCATCGA TCGTATTGAG AGCTTCTTTG GTGAACTGGG CTTCACCGTT
            661 GCGACCGGTC CGGAGATCGA AGACGATTAT CACAACTTTG ATGCGCTGAA CATTCCGGGT
            721 CACCACCCGG CGCGTGCGGA CCACGATACC TTCTGGTTTG ATACNACCCG TCTGCTGCGT
            781 ACCCAGANCA GCGGTGTTCN AATCCGTACC ATGAAGGCGC AGCAACCGCC GANTCGTATC
            841 ATTGCGCCGG GCCGTGTGTA CCGNNNCGAC TATGATCAGA NCCACACCCN NATGTTCCNN
            901 CNAATGGANG GNCTGNTCGN GGNNNCCNAC NTTANCTTCN NNNACCNGAA AGGNANCCTG
            961 NNCNANTTNN TGCNTNNCNN NNTTGANNNN NNNNNGNNNA NTNNNTTNNN NNNNNANNNN
          1021 NNTNNCNNNT TNNNNNNNNN N
          //
          Primer: ePAG_R1
          Region: mPAG2 3'
          Sequence file: RDB18374_B0H5b.seq check
          >D07025A3_B0H5_1_ePAG_R1_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNANNN GNNCTTNNCC TAGNNCCTTT TAAATTAAAA ATGAAGTTTT
             61 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GGAATTATTA CTTAAATTGT
            121 TTCAGGAAGC GCAGATCGTT TTCAAAGAAG CTACGCAGGT CGGTCACGCC ATAACGCAGC
            181 ATGGTCAGAC GTTCCATGCC CATACCAAAG CCGAAACCGC TGTACACTTC CGGATCAATA
            241 CCCACGTTAC GCAGAACGTT CGGGTGCACC ATGCCGCAAC CCAGAACTTC CAGCCATTTG
            301 CCGTTCTTGC CCATAACGTC CACTTCCGCG CTCGGCTCCG CAAACGGGAA GTAGCTCGGA
            361 CGAAAACGAA TCTGCAGATC TTCCTCAAAG AAGTTACGCA GAAAGTCGTG CAGGGTGCCT
            421 TTCAGGTTGG TGAAGCTAAT GTTGGTGTCC ACGATCAGAC CCTCCATTTG GTGGAACATC
            481 GGGGTGTGGG TCTGATCATA GTCGTTACGG TACACACGGC CCGGCGCAAT GATACGAATC
            541 GGCGGTTGCT GCGCCTTCAT GGTACGGATT TGAACACCGC TGGTCTGGGT ACGCAGCAGA
            601 CGGGTGGTAT CAAACCAGAA GGTATCGTGG TCCGCACGCG CCGGGTGGTG ACCCGGAATG
            661 TTCAGCGCAT CAAAGTTGTG ATAATCGTCT TCGATCTCCG GACCGGTCGC AACGGTGAAG
            721 CCCAGTTCAC CAAAGAAGCT CTCAATACGA TCGATGGTAC GGGTCACCGG GTGCAGGCCA
            781 CCGTTTTCNA TACGACGANC CGGCANGCTA ACGTCAATGG TCTCCGCCGC CAGANGCGCG
            841 TTCAGCGCCG CGCTTTCCAG CTCCGCTTNA CGCGCGTTCA GCGCTNGCTG CACTTGNNCC
            901 TTCNNNNCGT TGANAANCGC ACCCGCCGCN NGANGTTCCT CCGGCNNNNN TTCACGCAGG
            961 GNGGNCATCT GCNNGGNCNN NTGNNNTTTN NNANCCNNGN NNTNNNNNNG CACANTGTCN
          1021 NNCNNNNNNN NNN
          //
          Primer: pGEX3'
          Region: AmpR pro, cat
          Sequence file: RDB18374_B0H5c.seq check
          >D07025A3_B0H5_1_pGEX3'_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGCCTCG TGANACGCCT ATTTTTATAG GTTAATGTCA
             61 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
            121 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
            181 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGGA GAAAAAAATC ACTGGATATA
            241 CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG
            301 CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA
            361 AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG
            421 CTCATCCGGA ATTTCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC
            481 ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT
            541 ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG
            601 AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC
            661 CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCNA TATGGACAAC TTCTTCGCCC
            721 CCGTTTTCNC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA
            781 TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGANTGCTT AATGAATTAC
            841 ANNNNTACTG CNATGANTGG CNNGNCNGGG CGTAAAGGAN GTNAAACNCA TGTCACANCT
            901 ANCANAANNN NNTTGCTAGT GCNAAAGCTG NNATATCNCN ANGCTAGCGA TGTTGCNGCN
            961 CTNGNNNNAT GNGCGTGTGG NNTANNTGGG NNANAANNNN NNNCTNNNCN NNNNNANGAN
          1021 NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl