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pUC18Z_mPAG2 (#RDB18373)

Cloning vector harboring PheS counterselection marker. Zeocin resistance.


Drawn by SnapGene® software
Sequence (full) RDB18373hts01.seq
Publication Unpublished bioresource
Test sheet RDB18373_B0H5p1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. An improved clone of pUC18Z_ePAG2 (RDB17948). The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Zeocin, pheS
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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RDB18373 pUC18Z_mPAG2 DNA solution


How to cite this biological resource

Materials & Methods section:

The pUC18Z_mPAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18373).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP]