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pUC18Z_mPAG2

Cloning vector harboring PheS counterselection marker. Zeocin resistance.

Catalog number RDB18373
Resource name pUC18Z_mPAG2
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. An improved clone of pUC18Z_ePAG2 (RDB17948). The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Zeocin, pheS
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |
Other clones in our bank

External Database
Escherichia coli pheS

          Reference sequence
            
           
          Sequence (full) RDB18373zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。
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          RDB18373 pUC18Z_mPAG2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pUC18Z_mPAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18373).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          References and tips

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18373_B0H5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX3' (Pr0213)
          Region: Amp pro, ZeoR, insert 5'
          Sequence file: RDB18373_B0H5a.seq check
          >D07025A2_B0H5_1_pGEX3'_E04_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNCGNNN NNNNNGGCCT CGTGNNANGC CTATTTTTAT AGGTTAATGT
             61 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC
            121 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC
            181 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG GCCAAGTTGA CCAGTGCCGT
            241 TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG
            301 GTTCTCTCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT
            361 GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT
            421 GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA
            481 CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT
            541 GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG GAGCAGGACT AAAGGAGGTA
            601 AAACACATGT CACACCTAGC AGAATTAGTT GCTAGTGCAA AAGCTGCAAT ATCACAAGCT
            661 AGCGATGTTG CGGCGCTGGA CAATGTGCGT GTGGAGTACC TGGGTAAGAA AGGCCACCTG
            721 ACCCTGCAGA TGACCACCCT GCGTGAACTG CCGCCGGANG ANCGTCCGGC GGCGGGTGCG
            781 GTTATCAACG AGGCGAANGA NCAAGTGCAG CAAGCGCTGA ACGCGCGTNA AGCGGANCTG
            841 GAAAGCGCGG CGCTNAACGC GCGTCTGGCG GCGGANNNNA TTGACGTTAG CCTGNCNGGN
            901 CGTCNNATNG AAAACNGNGG CCTGNNCCNN GNGNNCCNNN NCATCNNTCG NNTTGAGANC
            961 NTCTTTNNNN AANNNGGNTT NNNNNNTNNC NNNCNGNNCN GNANNTNNNA NNNNNANNNN
          1021 NNNNNN
          //
          Primer: ePAG_R1 (Pr0828)
          Region: insert 3'
          Sequence file: RDB18373_B0H5b.seq check
          >D07025A2_B0H5_1_ePAG_R1_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNGGNNN TTNNCCTAGN NCCTTNNNNN TAAAAATGAA GTTTTAAATC
             61 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGGAAT TATTACTTAA ATTGTTTCAG
            121 GAAGCGCAGA TCGTTTTCAA AGAAGCTACG CAGGTCGGTC ACGCCATAAC GCAGCATGGT
            181 CAGACGTTCC ATGCCCATAC CAAAGCCGAA ACCGCTGTAC ACTTCCGGAT CAATACCCAC
            241 GTTACGCAGA ACGTTCGGGT GCACCATGCC GCAACCCAGA ACTTCCAGCC ATTTGCCGTT
            301 CTTGCCCATA ACGTCCACTT CCGCGCTCGG CTCCGCAAAC GGGAAGTAGC TCGGACGAAA
            361 ACGGATCTGC AGATCTTCCT CAAAGAAGTT ACGCAGAAAG TCGTGCAGGG TGCCTTTCAG
            421 GTTGGTGAAG CTAATGTTGG TGTCCACGAT CAGACCCTCC ATTTGGTGGA ACATCGGGGT
            481 GTGGGTCTGA TCATAGTCGT TACGGTACAC ACGGCCCGGC GCAATGATAC GAATCGGCGG
            541 TTGCTGCGCC TTCATGGTAC GGATTTGAAC ACCGCTGGTC TGGGTACGCA GCAGACGGGT
            601 GGTATCAAAC CAGAAGGTAT CGTGGTCCGC ACGCGCCGGG TGGTGACCCG GAATGTTCAG
            661 CGCATCAAAG TTGTGATAAT CGTCTTCGAT CTCCGGACCG GTCGCAACGG TGAAGCCCAG
            721 TTCACCAAAG AAGCTCTCAA TACGATCGAT GGTACGGGTC ACCGGGTGCA GGCCACCGTT
            781 TTCAATACGA CGACCCGGCA GGCTAACGTC AATGGTCTCC GCCGCCAGAN GCGCGTTCAG
            841 CGCCGCGCTT TCCAGCTCCG CTTTACGCGC GTTCAGCGCT TGCTGCACTT GTNCCTTCGC
            901 NNCGTTGANA NNCGCACCCG CCGCCNGNNG NNCCTCCGGC GGCAGTNCAC GCANGGNGGN
            961 CNTCTGCNNG GNCNNNTGGC CTTTNNNANC NNNGTNNNNN NNANGCNNNT TGNNNNNNNN
          1021 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl