Cloning vector harboring PheS counterselection marker. Zeocin resistance.
Drawn by SnapGene® software |
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Clone info. | Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. An improved clone of pUC18Z_ePAG2 (RDB17948). The risk of recombination is lower. |
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Comment | Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine. |
Vector backbone | pUC18 (plasmid) |
Selectable markers | Zeocin, pheS |
Gene/insert name | Escherichia coli pheS genomic DNA |
Depositor|Developer | Suzuki, Kaoru | Miyazaki, Kentaro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18373 | pUC18Z_mPAG2 | DNA solution |
Materials & Methods section:
The pUC18Z_mPAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18373). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
reference | Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [link to RRC of NBRP] |
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