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pUC18Z_mPAG2

Cloning vector harboring PheS counterselection marker. Zeocin resistance.

Catalog number RDB18373
Resource name pUC18Z_mPAG2
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Zeocin resistance. An improved clone of pUC18Z_ePAG2 (RDB17948). The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Zeocin, pheS
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |
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External Database
Escherichia coli pheS

          Reference sequence
            
           
          Sequence (full) RDB18373zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。
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          RDB18373 pUC18Z_mPAG2 Under QC test. Please contact us. DNA solution

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          References

          Original, user report and related articles

          reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032.

          2022.08.12

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