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pUC18K_mPAG2 (#RDB18372)

Cloning vector harboring PheS counterselection marker. Kanamycin resistance.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Kanamycin resistance. An improved clone of pUC18K_ePAG2 (RDB17947). The risk of recombination is lower.
Comment Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
Vector backbone pUC18 (plasmid)
Selectable markers Kanamycin, pheS
Growth conditions LB+Kanamycin, 37oC
Gene/insert name Escherichia coli pheS genomic DNA
Depositor|Developer Suzuki, Kaoru | Miyazaki, Kentaro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。

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RDB18372 pUC18K_mPAG2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pUC18K_mPAG2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18372).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Miyazaki, K., Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli. Biotechniques 58 (2): 86-88 (2015). PMID 25652032. [PubMed] [Article] [RRC of NBRP]