Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC18K_mPAG2

Cloning vector harboring PheS counterselection marker. Kanamycin resistance.

Sequence (full)	RDB18372zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18372_B0H5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: KanR-C_F (Pr0827) Region: KanR 3', mPAG2 5' Sequence file: RDB18372_B0H5a.seq
>D07025A1_B0H5_3_KanR-C_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNAANN NNGNTNNTGA NAATCCTGNT ATGAATAAAT TGCAGTTTCA    61 TTTGATGCTC GATGAGTTTT TCTAAAGGAG GTAAAACACA TGTCACACCT AGCAGAATTA   121 GTTGCTAGTG CAAAAGCTGC AATATCACAA GCTAGCGATG TTGCGGCGCT GGACAATGTG   181 CGTGTGGAGT ACCTGGGTAA GAAAGGCCAC CTGACCCTGC AGATGACCAC CCTGCGTGAA   241 CTGCCGCCGG AGGAACGTCC GGCGGCGGGT GCGGTTATCA ACGAGGCGAA GGAACAAGTG   301 CAGCAAGCGC TGAACGCGCG TAAAGCGGAG CTGGAAAGCG CGGCGCTGAA CGCGCGTCTG   361 GCGGCGGAGA CCATTGACGT TAGCCTGCCG GGTCGTCGTA TTGAAAACGG TGGCCTGCAC   421 CCGGTGACCC GTACCATCGA TCGTATTGAG AGCTTCTTTG GTGAACTGGG CTTCACCGTT   481 GCGACCGGTC CGGAGATCGA AGACGATTAT CACAACTTTG ATGCGCTGAA CATTCCGGGT   541 CACCACCCGG CGCGTGCGGA CCACGATACC TTCTGGTTTG ATACCACCCG TCTGCTGCGT   601 ACCCAGACCA GCGGTGTTCA AATCCGTACC ATGAAGGCGC AGCAACCGCC GATTCGTATC   661 ATTGCGCCGG GCCGTGTGTA CCGTAACGAC TATGATCAGA CCCACACCCC GATGTTCCAC   721 CAAATGGAGG GTCTGATCGT GGACACCAAC ATTAGCTTCA CCAACCTGAA AGGCACCCTG   781 CACGACTTTC TGCGTAACTT CTTTGANGAA GATCTGCAGA TCCGTTTTCG TCCGANCTAC   841 TTCCCGTTTG CGGANCCNAN CGCGGAAGTG NANGTTATGG GCAANAACGG CAAATGGCTG   901 GANNTTCTGG GTTGCGGNAT GGNGCACCNG ANCGTTCTGC NNAACGTGGG NATNGNNNCN   961 GNANNNGTAC AGNNGNTTTC NGCTTNNGNT ATNGGNNNNG NNNNGNNNGA NNNNNCNNNN 1021 NNNNNN //
Primer: ePAG_R1 (Pr0828) Region: mPAG2 3' Sequence file: RDB18372_B0H5b.seq
>D07025A1_B0H5_ePAG_R1_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNAANN GNNCTTNNNC TAGNTCCTTT TAAATTAAAA ATGAAGTTTT    61 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GGAATTATTA CTTAAATTGT   121 TTCAGGAAGC GCAGATCGTT TTCAAAGAAG CTACGCAGGT CGGTCACGCC ATAACGCAGC   181 ATGGTCAGAC GTTCCATGCC CATACCAAAG CCGAAACCGC TGTACACTTC CGGATCAATA   241 CCCACGTTAC GCAGAACGTT CGGGTGCACC ATGCCGCAAC CCAGAACTTC CAGCCATTTG   301 CCGTTCTTGC CCATAACGTC CACTTCCGCG CTCGGCTCCG CAAACGGGAA GTAGCTCGGA   361 CGAAAACGGA TCTGCAGATC TTCCTCAAAG AAGTTACGCA GAAAGTCGTG CAGGGTGCCT   421 TTCAGGTTGG TGAAGCTAAT GTTGGTGTCC ACGATCAGAC CCTCCATTTG GTGGAACATC   481 GGGGTGTGGG TCTGATCATA GTCGTTACGG TACACACGGC CCGGCGCAAT GATACGAATC   541 GGCGGTTGCT GCGCCTTCAT GGTACGGATT TGAACACCGC TGGTCTGGGT ACGCAGCAGA   601 CGGGTGGTAT CAAACCAGAA GGTATCGTGG TCCGCACGCG CCGGGTGGTG ACCCGGAATG   661 TTCAGCGCAT CAAAGTTGTG ATAATCGTCT TCGATCTCCG GACCGGTCGC AACGGTGAAG   721 CCCAGTTCAC CAAAGAAGCT CTCAATACGA TCGATGGTAC GGGTCACCGG GTGCAGGCCA   781 CCGTTTTCNA TACGACGANC CGGCANGCTA ACGTCNATGG TCTCCGCCGC CAGANGCGCG   841 TTCAGCGCCG CGCTTTCCAG CTNCGCTTTA CGCGCGTTCA GCGCTNGCTG CACTTGTNCC   901 TTCGCCNCGT TGANAANCGC ACCNGCCGCC NGANGTTCCN NNGGCGGCAG TTCNCGCNNG   961 GNGGNCATCT GCNNGNNNNN NTGGNNNTTT NNNANCNNNG NNANTNNNNA CNNNCATTGN 1021 CCNNNNNNNN NN //
Primer: pGEX3' (Pr0213) Region: AmpR pro, KanR5' Sequence file: RDB18372_B0H5c.seq
>D07025A1_B0H5_3_pGEX3'_E02_05_ABI08.ab1     1 NNNNNNNNNN NNNCGCNNNN NNNNNNGNCC TCGTGNNACG CCTATTTTTA TAGGTTAATG    61 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA   121 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC   181 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGCCATATT CAACGGGAAA   241 CGTCTTGCTC GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT   301 GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT GGGAAGCCCG   361 ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG   421 AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA   481 TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGGAAA ACAGCATTCC   541 AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC   601 TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC   661 GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG   721 ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT   781 TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG   841 AGGGGAAATT AATANGTTGT ATTGATGTTG GANGANNCGG ANTCGCANAN CGATACCANG   901 ATCTTGCCNT NCTATGGANN TGNCNCNGTG AGTTTTCTCC TTCNTTACNN AAACNGCTTT   961 TTCNAAAATA TGNNANTGAN NATCNNGANN NGNNNAAANN GNANNTTCNT TTNNATGNNC 1021 NNNNANNTTT TNNNNANNNN NNNNAANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl