Cloning vector harboring PheS counterselection marker. Kanamycin resistance.
|Clone info.||Cloning vector harboring counterselection codon-modified marker Escherichia coli phenylalanyl-tRNA synthetase variant (T251A/A294G; mPAG2), a-subunit (ePheS) when 4-chloro-phenylalanine (4CP) is present. Kanamycin resistance. An improved clone of pUC18K_ePAG2 (RDB17947). The risk of recombination is lower.
Version with reduce recombination against PheS gene in E. coli genome. A tendency to host cell death in the presence of 1-10% (w/v) 4-chlorophenylalanine.
|Vector backbone||pUC18 (plasmid)|
|Selectable markers||Kanamycin, pheS|
|Gene/insert name||Escherichia coli pheS genomic DNA|
|Depositor|Developer||Suzuki, Kaoru | Miyazaki, Kentaro ||
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
|Terms and conditions set forth by the DEPOSITOR||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) |
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|提供条件||利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyazaki K. Biotechniques, 58 (2): 86-88, 2015) 。|
|Catalog #||Resource name||Availability||Shipping form||Fee (non-profit org.)|
|RDB18372||pUC18K_mPAG2||Under QC test. Please contact us.||DNA solution|
|Electronic file||Nucleotide sequence provided by the depositor RDB18372zzk01.seq|
Further references such as user reports and related articles (go to bottom)
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles