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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mitoKeima-Red (#RDB18369)

Expression vector encoding mitochondrially targeted Keima-Red (mitoKeima-Red).

Clone info. Expression vector encoding mitochondrially targeted Keima-Red (mitoKeima-Red). The mitochondria-targeting amino acid sequence MLSLRQSIRFFKPATRTLCSSR, derived from cytochrome oxidase subunit IV.
Comment Expression was confirmed by the depositor with fluorescence live cell imaging.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name Montipora sp. red fluorescent protein cDNA
Depositor|Developer Aoki, Shigeki |
 
Sequence (full) RDB18369zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shiratori, R. et al. Sci. Rep.10; 9(1): 18699, 2019)
Additional terms and conditions:
A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the Medical & Biological Laboratories Co., Ltd prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shiratori, R. et al. Sci. Rep.10; 9(1): 18699, 2019)。
MTAに書く付加的使用条件：
非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合はMBL社から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18369 mitoKeima-Red DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The mitoKeima-Red was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18369).

Reference section:

Shiratori, R., Furuichi, K., Yamaguchi, M., Miyazaki, N., Aoki, H., Chibana, H., Ito, K., Aoki, S., Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner. Sci. Rep. 9 (1): 18699 (2019). PMID 31822748. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18369_B0Gmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, mitoKeima-Red 5'
Sequence file: RDB18369_B0Gma.seq check
>D07172A1_B0Gm_3_CMV_Forward_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTG NNGCNNTGGG CGGTAGGCGT GTNCGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTTG CCATGCTGAG CCTGCGCCAG
  181 AGTATCCGCT TCTTCAAGCC CGCCACCAGG ACTCTGTGCA GTTCCAGGAT GGTGAGCGTG
  241 ATCGCCAAGC AGATGACCTA CAAGGTGTAC ATGAGCGGCA CCGTGAACGG CCACTACTTC
  301 GAGGTGGAGG GCGACGGCAA GGGCAAGCCC TACGAGGGCG AGCAGACCGT GAAGCTGACC
  361 GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGAGCCCCCA GCTCCAGTAC
  421 GGCAGCATCC CCTTCACCAA GTACCCCGAG GACATCCCCG ACTACTTCAA GCAGAGCTTC
  481 CCCGAGGGCT ACACCTGGGA GCGCAGCATG AACTTCGAGG ACGGCGCCGT GTGCACCGTG
  541 AGCAACGACA GCAGCATCCA GGGCAACTGC TTCATCTACA ACGTGAAGAT CAGCGGCGAG
  601 AACTTCCCCC CCAACGGCCC CGTGATGCAG AAGAAGACCC AGGGCTGGGA GCCCAGCACC
  661 GAGCGCCTGT TCGCCCGCGA CGGAATGCTG ATCGGCAACG ACTACANNNN NNTGAAGCTG
  721 GAGGGCGGCG GCCACTACCT GTGCGAGTTC AAGAGCACCT ACAANGCCAA GAAGCCCGTG
  781 ANGATGCCCG GCCGCCACGA NATCGANCGC AAGCTGGACG TGACCAGCCA CAACCGCGAC
  841 TACACCAGCG TGGANCAGTG CGANATCGCC ATCGCCCGCC NCANCCTGCT GGGCTNATCT
  901 AGANGGCCNG NNNNTCNAAN GNAAGCCTAT CCCTAACCNT NNNNNCGNNC TCNANTCNAC
961 GCNNANNGGN CATCNTCNNN NTCNNNNNTN NANNTNNANN CNNNNNNNNN N
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, mitoKeima-Red 3'
Sequence file: RDB18369_B0Gmb.seq check
>D07172A1_B0Gm_3_BGH_rev2_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNGNNGGGCA NCAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTACGCGTA
  121 GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG GCCCTCTAGA
  181 TTAGCCCAGC AGGCTGTGGC GGGCGATGGC GATCTCGCAC TGCTCCACGC TGGTGTAGTC
  241 GCGGTTGTGG CTGGTCACGT CCAGCTTGCG GTCGATCTCG TGGCGGCCGG GCATCCTCAC
  301 GGGCTTCTTG GCCTTGTAGG TGCTCTTGAA CTCGCACAGG TAGTGGCCGC CGCCCTCCAG
  361 CTTCAGGGCC ATGTAGTCGT TGCCGATCAG CATTCCGTCG CGGGCGAACA GGCGCTCGGT
  421 GCTGGGCTCC CAGCCCTGGG TCTTCTTCTG CATCACGGGG CCGTTGGGGG GGAAGTTCTC
  481 GCCGCTGATC TTCACGTTGT AGATGAAGCA GTTGCCCTGG ATGCTGCTGT CGTTGCTCAC
  541 GGTGCACACG GCGCCGTCCT CGAAGTTCAT GCTGCGCTCC CAGGTGTAGC CCTCGGGGAA
  601 GCTCTGCTTG AAGTAGTCGG GGATGTCCTC GGGGTACTTG GTGAAGGGGA TGCTGCCGTA
  661 CTGGAGCTGG GGGCTCAGGA TGTCCCAGGC GAAGGGCAGG GGGCCACCCT TGGTCACGGT
  721 CAGCTTCACG GTCTGCTCGC CCTCGTANGG CTTGCCCTTG CCGTCGCCCT CCACCTCGAA
  781 GTAGTGNNCG TTCACGGTGC CGCTCATGTA CACCTTGTAN GTCATCTGCT TGGCGATCAC
  841 GCTCACCATC CTGNAANTGC ACAGANNCCT GGTGNCGGGC TNGAAGAANC GGATACTCNG
  901 GNGCAGGNTC ANCATGGNNA GCNNAACNNN CCAGCTTGGG NCTCCNNATA NNGANTCNNA
961 NTAATTTCNA NNNNCNANTN ANCNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR
Sequence file: RDB18369_B0Gmc.seq check
>D07172A1_B0Gm_3_SV40pro_ori_F_G04_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCCTCTGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
  721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
  781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACATAGCGTT GGCTANCCGT GATATTGCTG
  841 AAGAGCTTGG CGGCGAANGG GCTGANCGCT TCCTCGNGCT TNNCGGNATC GCCGCNNCCG
  901 ANTCNCANCG CANCGNCNNN CTATCGCNTN CTNNNNNANN TNNNCTGANC GGNANTCNNG
961 GGGNNCNNNA AATGACCNNN CNANNCGANN NCNNNNCNNG NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shiratori, R., Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner. Sci. Rep. 9 (1): 18699 (2019). PMID 31822748. [link to RRC of NBRP]