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CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red)

CoralHue™ Mitochondria-targeted mKeima-Red (pMT-mKeima-Red)

Catalog number RDB18321
Resource name CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red)
Alternative name AM-V0251M
Clone info. ・For mammalian expression of the Mitochondria-targeted fluorescent protein.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone pMT (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18321 CoralHue(TM) Mitochondria-targeted mKeima-Red (pMT-mKeima-Red) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB18321zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18321_map.png

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18321_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, insert 5'(MT Targeted mKeima-Red 5')
            Sequence file: RDB18321_B0H4a.seq check
            >18321_18321_B1KgF_CMV-Forward_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNCGTGTACG GTGGGNGGTC
               61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCATTGCCA CCATGCTGAG
              121 CCTGCGCCAG AGTATCCGCT TCTTCAAGCC CGCCACCAGG ACTCTGTGCA GTTCCAGGGC
              181 GGCCGCGGGG ACAATGGTGA GTGTGATCGC TAAACAAATG ACCTACAAGG TTTATATGTC
              241 AGGCACGGTC AATGGACACT ACTTTGAGGT CGAAGGCGAT GGAAAAGGAA AGCCTTACGA
              301 GGGAGAGCAG ACAGTAAAGC TCACTGTCAC CAAGGGTGGA CCTCTGCCAT TTGCTTGGGA
              361 TATTTTATCA CCACAGCTTC AGTACGGAAG CATACCATTC ACCAAGTACC CTGAAGACAT
              421 CCCTGATTAT TTCAAGCAGT CATTCCCTGA GGGATATACA TGGGAGAGGA GCATGAACTT
              481 TGAAGATGGT GCAGTGTGTA CTGTCAGCAA TGATTCCAGC ATCCAAGGCA ACTGTTTCAT
              541 CTACAATGTC AAAATCTCTG GTGAGAACTT TCCTCCCAAT GGACCTGTTA TGCAGAAGAA
              601 GACACAGGGC TGGGAACCCA GCACTGAGCG TCTCTTTGCA CGAGATGGAA TGCTGATAGG
              661 AAACGATTAT ATGGCTCTGA AGTTGGAAGG AGGTGGTCAC TATTTGTGTG AATTTAAATC
              721 TACTTACAAG GCANAGAAGC CTGTGANGAT GCCAGGGCGC CACGAGATTG ACCGCNAACT
              781 GGATGTAACC AGTCACAACA GGGATTACAC ATCTGTTGAG CAGTGTGAAA TAGCCATTGC
              841 ACGCCACTCT TNGCTCGGNT AANCTAGANG GGGGCCGCNA NTCTNGATCA TAATCAGCCN
              901 TACCNNATTT GNAGANGTTT TACTTGCTTT NAAAANNNNN NCNNCANCNT NCCNCNNNNA
            961 NNNNNNANNN NNNNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'(MT Targeted mKeima-Red 3')
            Sequence file: RDB18321_B0H4b.seq check
            >D07039I9_B0H4_4_SV40polyA-R2_E08_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNAANNT TAANNACNAC NATTGCATTC ATTTTATGTT
               61 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
              121 TATGGCTGAT TATGATCTAG AGTCGCGGCC CCCTCTAGAT TAACCGAGCA AAGAGTGGCG
              181 TGCAATGGCT ATTTCACACT GCTCAACAGA TGTGTAATCC CTGTTGTGAC TGGTTACATC
              241 CAGTTTGCGG TCAATCTCGT GGCGCCCTGG CATCCTCACA GGCTTCTTTG CCTTGTAAGT
              301 AGATTTAAAT TCACACAAAT AGTGACCACC TCCTTCCAAC TTCAGAGCCA TATAATCGTT
              361 TCCTATCAGC ATTCCATCTC GTGCAAAGAG ACGCTCAGTG CTGGGTTCCC AGCCCTGTGT
              421 CTTCTTCTGC ATAACAGGTC CATTGGGAGG AAAGTTCTCA CCAGAGATTT TGACATTGTA
              481 GATGAAACAG TTGCCTTGGA TGCTGGAATC ATTGCTGACA GTACACACTG CACCATCTTC
              541 AAAGTTCATG CTCCTCTCCC ATGTATATCC CTCAGGGAAT GACTGCTTGA AATAATCAGG
              601 GATGTCTTCA GGGTACTTGG TGAATGGTAT GCTTCCGTAC TGAAGCTGTG GTGATAAAAT
              661 ATCCCAAGCA AATGGCAGAG GTCCACCCTT GGTGACAGTG AGCTTTACTG TCTGCTCTCC
              721 CTCGTAAGGC TTTCCTTTTC CATCGCCTTC GACCTCAAAG TAGTGTCCAT TGACCGTGCC
              781 TGACATATAA ACCTTGTANG TCATTTGTTT AGCGATCACA CTCACCATTG TCCCCGCGGC
              841 CGCCCTGNAA CTGCACAGAN TCCTGGTGGC GGGCTTGAAG AAGCGNANAC TCTGGCGCNN
              901 NTCNNCATGG NGGCNANGCT AGCGGATCTN ANGGTNCACN NAACCAGCTC TGCTNATATA
            961 NANNTCCCNC NNNACACGCC NANCNNCNNT TTGCGTCNAN GGGGNNNNNN NNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB18321_B0H4c.seq check
            >D07039I9_B0H4_4_SV40pro_ori_F_E09_15_ABI24.ab1
                1 NNNNNNNNNN NNNGNNGNCN CCTCNNCCTC TGAGCTATTC CNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCA TCAAGAGACA GGATGAGGAT CGTTTCGCAT
              121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG
              181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC
              241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
              301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT
              361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA
              421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG
              481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT
              541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
              601 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG
              661 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG
              721 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT
              781 ANCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGNCGGC GAATGNGCTG ANCGCTTCCT
              841 CGNGCTTTAC NGTATCGCCG CNCCCNATTC NCAGCGCATC GCCTTCTATC GCCTTCTTGA
              901 NNANTTNTTC TGANCGGGAN TCNNGGGGTT CGAAATGACC GANCNANCGA CGCCCNNCNN
            961 GNCNTCNNNA GATTTCNNNT NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2021.12.03

            GNP_filter3_RDBDEP_html_211111.pl