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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1) (#RDB18317)

CoralHue™ ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1)

Alternative name AM-V0222M
Clone info. ・For mammalian expression of the ER-targeted fluorescent protein.
・Monomeric Kusabira-Orange1 is Orange fluorescent protein.
・Ex/Em(nm): 548/561
Vector backbone pER (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18317zzk01.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18317 CoralHue(TM) ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18317).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18317_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, mKO1 5'
Sequence file: RDB18317_B0H4a.seq check
>D07039I5_B0H4_1_CMV_Forward_D07_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CNNNGGGCGG TANGCGTGTA CGGNGGGNNG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCATTGC CACCATGCTG CTGCCCGTCC
  121 CCCTGCTGCT GGGCCTGCTG GGCGCCGCCG CGGATCCGAT GGTGAGTGTG ATTAAACCAG
  181 AGATGAAGAT GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATTGAAG
  241 GTGAAGGCAC AGGCAGACCT TACGAGGGAC ATCAAGAGAT GACACTACGC GTCACAATGG
  301 CCAAGGGCGG GCCAATGCCT TTCGCGTTTG ACTTAGTGTC ACACGTGTTC TGTTACGGCC
  361 ACAGACCTTT TACTAAATAT CCAGAAGAGA TACCAGACTA TTTCAAACAA GCATTTCCTG
  421 AAGGCCTGTC ATGGGAAAGG TCGTTGGAGT TCGAAGATGG TGGGTCCGCT TCAGTCAGTG
  481 CGCATATAAG CCTTAGAGGA AACACCTTCT ACCACAAATC CAAATTTACT GGGGTTAACT
  541 TTCCTGCCGA TGGTCCTATC ATGCAAAACC AAAGTGTTGA TTGGGAGCCA TCAACCGAGA
  601 AAATTACTGC CAGCGACGGA GTTCTGAAGG GTGATGTTAC GATGTACCTA AAACTTGAAG
  661 GANGCGGCAA TCACAAATGC CAATTCAAGA CTACTTACAA NGCGGCAAAA AAGANTCTTA
  721 AAATGNCAGG AANCCATTAC ATCAGCCATC GCCTCGTCAG GAAAACCGAA NGCNNCATTA
  781 CTGANCTGGT ANAANATGCA GTANCTCATT CCNAGGANGA NCTGTNATCT ANANGGGGGC
  841 CGCNANNCTA NATCATAATC AGCNNTANNA CATTTGNANA NGNTTNANNN NNNTNNAAAA
901 AANCNTCCNN CANNNNNNNN NN
//
Primer: SV40polyA_R2 (Pr0623)
Region: mKO1 3'
Sequence file: RDB18317_B0H4b.seq check
>D07039I5_B0H4_1_SV40polyA-R2_D08_11_ABI24.ab1
    1 NNNNNNNNCN NCNNNNNAGT TNNCANNAAC AATTGNATTC NTTTTATGTT TCNGGTTCAG
   61 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
  121 TATGATCTAG AGTCGCGGCC CCCTCTAGAT TACAGCTCGT CCTTGGAATG AGCTACTGCA
  181 TCTTCTACCA GCTCAGTAAT GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCTGATGTAA
  241 TGGCTTCCTG GCATTTTAAG AATCTTTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT
  301 TTGTGATTGC CGCCTCCTTC AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG
  361 TCGCTGGCAG TAATTTTCTC GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA
  421 GGACCATCGG CAGGAAAGTT AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT
  481 CTAAGGCTTA TATGCGCACT GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT
  541 TCCCATGACA GGCCTTCAGG AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT
  601 TTAGTAAAAG GTCTGTGGCC GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAANGC
  661 ATTGGCCCGC CCTTGGCCAT TGTGACGCGT ANTGTCATCT CTTGATGTCC CTCGTAAGGT
  721 CTGCCTGTGC CTTCACCTTC NATTGTGAAC TCATGCCCAT TGACGGANCC GTCCATGTAG
  781 TACCTCATCT TCATCTCTGG TTTAATCACA CTCACCATCG GNNCCGCGGC GGCGCCNANC
  841 ANGNCCAGCA GCAGGGGGNN NNNCAGCANC ATNGNGGCAN NGCTANCGNN TNNNNNGGNN
901 CNNNNAACCA NNNNNNCNNN NATANNNNNN CNNNNNNNNN N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB18317_B0H4c.seq check
>D07039I5_B0H4_1_SV40pro_ori_F_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNGCCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGN NGCTTTTTTG
   61 GAGGCCTAGG CTTTTGCAAA GATCATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG
  121 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG
  181 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG
  241 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG
  301 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG
  361 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC
  421 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC
  481 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC
  541 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAANAGCATC
  601 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG
  661 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT
  721 TTTCTGGATT CATCGACTGT GGCCGGCTGG GNGTGGCGGA NCGCTATCAN GACATAGCGT
  781 TGGCTANNNG NNNTATTGCT GAANNNCTTG GCGGCGAATG GGCTGANCGC TTCCNCNNGC
  841 TTTACNGNAN CGCCGCNCCN NNNTNNCANC GCATCGCCNT CNNNCNNNNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]