CoralHue™ ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1)
Alternative name | AM-V0222M |
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Clone info. | ・For mammalian expression of the ER-targeted fluorescent protein. ・Monomeric Kusabira-Orange1 is Orange fluorescent protein. ・Ex/Em(nm): 548/561 |
Vector backbone | pER (plasmid) |
Size of vector backbone | 3.9 kb |
Selectable markers | Kanamycin (E. coli), Neomycin (mammalian cells) |
Gene/insert name | Fungia concinna orange fluorescent protein cDNA |
Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Sequence (full) | RDB18317zzk01.seq provided by the depositor |
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Additional map |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18317 | CoralHue(TM) ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1) | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The CoralHue(TM) ER-targeted monomeric Kusabira-Orange 1 (pER-mKO1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18317). |
Reference section:
Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18317_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, mKO1 5' Sequence file: RDB18317_B0H4a.seq |
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>D07039I5_B0H4_1_CMV_Forward_D07_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG CNNNGGGCGG TANGCGTGTA CGGNGGGNNG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCATTGC CACCATGCTG CTGCCCGTCC 121 CCCTGCTGCT GGGCCTGCTG GGCGCCGCCG CGGATCCGAT GGTGAGTGTG ATTAAACCAG 181 AGATGAAGAT GAGGTACTAC ATGGACGGCT CCGTCAATGG GCATGAGTTC ACAATTGAAG 241 GTGAAGGCAC AGGCAGACCT TACGAGGGAC ATCAAGAGAT GACACTACGC GTCACAATGG 301 CCAAGGGCGG GCCAATGCCT TTCGCGTTTG ACTTAGTGTC ACACGTGTTC TGTTACGGCC 361 ACAGACCTTT TACTAAATAT CCAGAAGAGA TACCAGACTA TTTCAAACAA GCATTTCCTG 421 AAGGCCTGTC ATGGGAAAGG TCGTTGGAGT TCGAAGATGG TGGGTCCGCT TCAGTCAGTG 481 CGCATATAAG CCTTAGAGGA AACACCTTCT ACCACAAATC CAAATTTACT GGGGTTAACT 541 TTCCTGCCGA TGGTCCTATC ATGCAAAACC AAAGTGTTGA TTGGGAGCCA TCAACCGAGA 601 AAATTACTGC CAGCGACGGA GTTCTGAAGG GTGATGTTAC GATGTACCTA AAACTTGAAG 661 GANGCGGCAA TCACAAATGC CAATTCAAGA CTACTTACAA NGCGGCAAAA AAGANTCTTA 721 AAATGNCAGG AANCCATTAC ATCAGCCATC GCCTCGTCAG GAAAACCGAA NGCNNCATTA 781 CTGANCTGGT ANAANATGCA GTANCTCATT CCNAGGANGA NCTGTNATCT ANANGGGGGC 841 CGCNANNCTA NATCATAATC AGCNNTANNA CATTTGNANA NGNTTNANNN NNNTNNAAAA 901 AANCNTCCNN CANNNNNNNN NN // |
Primer: SV40polyA_R2 (Pr0623) Region: mKO1 3' Sequence file: RDB18317_B0H4b.seq |
>D07039I5_B0H4_1_SV40polyA-R2_D08_11_ABI24.ab1 1 NNNNNNNNCN NCNNNNNAGT TNNCANNAAC AATTGNATTC NTTTTATGTT TCNGGTTCAG 61 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT 121 TATGATCTAG AGTCGCGGCC CCCTCTAGAT TACAGCTCGT CCTTGGAATG AGCTACTGCA 181 TCTTCTACCA GCTCAGTAAT GTTGCCTTCG GTTTTCCTGA CGAGGCGATG GCTGATGTAA 241 TGGCTTCCTG GCATTTTAAG AATCTTTTTT GCCGCCTTGT AAGTAGTCTT GAATTGGCAT 301 TTGTGATTGC CGCCTCCTTC AAGTTTTAGG TACATCGTAA CATCACCCTT CAGAACTCCG 361 TCGCTGGCAG TAATTTTCTC GGTTGATGGC TCCCAATCAA CACTTTGGTT TTGCATGATA 421 GGACCATCGG CAGGAAAGTT AACCCCAGTA AATTTGGATT TGTGGTAGAA GGTGTTTCCT 481 CTAAGGCTTA TATGCGCACT GACTGAAGCG GACCCACCAT CTTCGAACTC CAACGACCTT 541 TCCCATGACA GGCCTTCAGG AAATGCTTGT TTGAAATAGT CTGGTATCTC TTCTGGATAT 601 TTAGTAAAAG GTCTGTGGCC GTAACAGAAC ACGTGTGACA CTAAGTCAAA CGCGAAANGC 661 ATTGGCCCGC CCTTGGCCAT TGTGACGCGT ANTGTCATCT CTTGATGTCC CTCGTAAGGT 721 CTGCCTGTGC CTTCACCTTC NATTGTGAAC TCATGCCCAT TGACGGANCC GTCCATGTAG 781 TACCTCATCT TCATCTCTGG TTTAATCACA CTCACCATCG GNNCCGCGGC GGCGCCNANC 841 ANGNCCAGCA GCAGGGGGNN NNNCAGCANC ATNGNGGCAN NGCTANCGNN TNNNNNGGNN 901 CNNNNAACCA NNNNNNCNNN NATANNNNNN CNNNNNNNNN N // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR_KanR Sequence file: RDB18317_B0H4c.seq |
>D07039I5_B0H4_1_SV40pro_ori_F_D09_12_ABI24.ab1 1 NNNNNNNNNN NNNNGCCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGN NGCTTTTTTG 61 GAGGCCTAGG CTTTTGCAAA GATCATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG 121 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG 181 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG 241 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG 301 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG 361 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 421 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC 481 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC 541 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAANAGCATC 601 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG 661 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 721 TTTCTGGATT CATCGACTGT GGCCGGCTGG GNGTGGCGGA NCGCTATCAN GACATAGCGT 781 TGGCTANNNG NNNTATTGCT GAANNNCTTG GCGGCGAATG GGCTGANCGC TTCCNCNNGC 841 TTTACNGNAN CGCCGCNCCN NNNTNNCANC GCATCGCCNT CNNNCNNNNT NNNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP] |
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