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CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1) (#RDB18314)

CoralHue™ β-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)

Alternative name AM-V0205M
Clone info. ・For mammalian expression of the β‐Actin-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone pActin (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18314zzk01.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18314 CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18314).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18314_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert (mAG1 5')
Sequence file: RDB18314_B0H4a.seq check
>D07039I2_B0H4__2_CMV_Forward_A06_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNGCNNATG GGCGGTANGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC ATTGCCACCA TGGTGAGTGT
  121 GATTAAACCA GAGATGAAGA TCAAGCTGTG TATGAGAGGC ACTGTAAACG GGCATAATTT
  181 CGTGATTGAA GGAGAAGGAA AAGGAAATCC TTACGAGGGA ACGCAGATTT TAGACCTGAA
  241 CGTCACTGAA GGCGCACCTC TGCCTTTCGC TTACGATATC TTGACAACAG TGTTCCAGTA
  301 CGGCAACAGG GCATTCACCA AGTACCCAGC AGATATTCAG GACTATTTCA AGCAGACTTT
  361 TCCTGAGGGG TATCACTGGG AAAGAAGCAT GACTTATGAA GACCAGGGCA TTTGCACCGC
  421 CACAAGCAAC ATAAGCATGA GGGGCGACTG TTTTTTCTAT GACATTCGTT TTGATGGCAC
  481 CAACTTTCCT CCCAATGGTC CGGTTATGCA GAAGAAGACT CTTAAATGGG AGCCATCCAC
  541 TGAGAAAATG TACGTAGAGG ATGGAGTGCT GAAGGGTGAT GTTAACATGC GCCTGTTGCT
  601 TGAAGGAGGT GGCCATTATC GATGTGATTT CAAAACTACT TACAAAGCAA AGAAGGAGGT
  661 CCGTTTGCCA GACGCGCACA AAATTGACCA CCGCATTGAG ATTTTGAAGC ATGACAAAGA
  721 TTACAACAAG GTCAAGCTCT ATGAGAATGC CGTTGCTCGC TATTCTATGC TGCCGAGTCA
  781 GGNCAAGACC GGTAATTCCG CTGACGGCGG CGGANGATCG GGTGGTAGTG GTGGTTCAGG
  841 ANGAGGANCG ANCCAAGGAN GNTCCATGGA TGANGATATC GCCGCGCTCG NCGTCGANNA
  901 NNGCTCCGGN ATGTGCAAGG NCGGNTNCGC GGGCGANNAT GCCCCCCGGG CCGTCTTCCC
  961 CNCCATCGNG GGGCGCCCCN NNNCNNNGGN NTGATGNNGG GNATGGGNCA NANNNANTCC
1021 TATGTGGNNN NNNANNNCNN NNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert (hActin beta 3')
Sequence file: RDB18314_B0H4b.seq check
>D07039I2_B0H4__2_SV40polyA_R2_B06_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAA CAACAANNAT TGCATTCNTT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCTAGAGT CGCGGCCGCT TAGAAGCATT TGCGGTGGAC GATGGAGGGG
  181 CCGGACTCGT CATACTCCTG CTTGCTGATC CACATCTGCT GGAAGGTGGA CAGCGAGGCC
  241 AGGATGGAGC CGCCGATCCA CACGGAGTAC TTGCGCTCAG GAGGAGCAAT GATCTTGATC
  301 TTCATTGTGC TGGGTGCCAG GGCAGTGATC TCCTTCTGCA TCCTGTCGGC AATGCCAGGG
  361 TACATGGTGG TGCCGCCAGA CAGCACTGTG TTGGCGTACA GGTCTTTGCG GATGTCCACG
  421 TCACACTTCA TGATGGAGTT GAAGGTAGTT TCGTGGATGC CACAGGACTC CATGCCCAGG
  481 AAGGAAGGCT GGAAGAGTGC CTCAGGGCAG CGGAACCGCT CATTGCCAAT GGTGATGACC
  541 TGGCCGTCAG GCAGCTCGTA GCTCTTCTCC AGGGAGGAGC TGGAAGCAGC CGTGGCCATC
  601 TCTTGCTCGA AGTCCAGGGC GACGTAGCAC AGCTTCTCCT TAATGTCACG CACGATTTCC
  661 CGCTCGGCCG TGGTGGTGAA GCTGTAGCCG CGCTCGGTGA GGATCTTCAT GAGGTAGTCA
  721 GTCAGGTCCC GGCCAGCCAG GTCCAGACGC AGGATGGCAT GGGGGAGGGC ATACCCCTCG
  781 TAGATGGGCA CAGTGTGGGT GANCCCGTCA CCGGANTCCA TCACGATGCC AGTGGTACGG
  841 CCAGAGGCGT ACAGGGNTAG CACAGCCTNN NTAGCNACGT ACATGGCTGG GGTGNTGAAN
  901 GTCTCAAACA TGATCTGGGN CATCTTCTCG CGNTTGGCCT TGGGGTTCNN NGGGCCNCNG
  961 NCANCANCAN GGGGNGNTNN TNGGGANNCN CANNCNNCNC NNNNNANNNG NNNNNNNGNC
1021 NNANTTTNNN NNNNNNN
//
Primer: mAG_MF1 (Pr1043)
Region: insert mid(mAG1 3',linker,hActin beta 5')
Sequence file: RDB18314_B0H4c.seq check
>D07039I2_B0H4__2_mAG_MF1_D06_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN CNNTAGAGGN NGGAGTGCTG AAGGGTGANG
   61 TTAACATGCG CCTGTTGCTT GAAGGAGGTG GCCATTATCG ATGTGATTTC AAAACTACTT
  121 ACAAAGCAAA GAAGGAGGTC CGTTTGCCAG ACGCGCACAA AATTGACCAC CGCATTGAGA
  181 TTTTGAAGCA TGACAAAGAT TACAACAAGG TCAAGCTCTA TGAGAATGCC GTTGCTCGCT
  241 ATTCTATGCT GCCGAGTCAG GCCAAGACCG GTAATTCCGC TGACGGCGGC GGAGGATCGG
  301 GTGGTAGTGG TGGTTCAGGA GGAGGATCGA CCCAAGGAGG ATCCATGGAT GATGATATCG
  361 CCGCGCTCGT CGTCGACAAC GGCTCCGGCA TGTGCAAGGC CGGCTTCGCG GGCGACGATG
  421 CCCCCCGGGC CGTCTTCCCC TCCATCGTGG GGCGCCCCAG GCACCAGGGC GTGATGGTGG
  481 GCATGGGTCA GAAGGATTCC TATGTGGGCG ACGAGGCCCA GAGCAAGAGA GGCATCCTCN
  541 NCCTGAAGTA CCCCATCGAG CACGGCATCG TCACCAACTG GGACGACATG GAGAAAATCT
  601 GGCACCACAC CTTCTACAAT GAGCTGCGTG TGGCTCCCGA GGAGCACCCC GTGCTGCTGA
  661 CCGAGGCCCC CCTGAACNNN NNNGCCAACC GCGAGAAGAT GACCCAGATC ATGTTTGAGA
  721 CCTTCAACAC CCCAGCCATG TACGTTGCTA TCCAGGNNGN NCNNNNCCTG TNCGCCTCTG
  781 GCCGTACCAC TGNNNNCGTG ATGGACTCCG GTGANGGGGT CACNCACNCT GTGCCCATCT
  841 ACGANGGGTA TGCCCTCCNN ATGCCATCCN GCGTCTGGAN CTGNCTGGNC NGGANCTGNN
  901 CTGANTACNN NNTGAANNNC CTCACCGANC GCNNNTACNG CTNCACCNCN NNNGNCNANC
  961 GGGAAATCNN NGNNTGNNAT TAANGANAAN NCTGTGCNNN NGTCNNCCNN GNNCTNNNNN
1021 NCANNNNNNN NGNNCNNNNN TGNNTNNCAG CNNCNTNCCN NNGNANNAAN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB18314_B0H4d.seq check
>D07039I2_B0H4__2_SV40pro_ori_F_C06_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNC GCCTCNGCCT CTGNNCTATT CCNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCAGGACA
  781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGNGCT GANCGCTTCC
  841 TCGNGCTTTA CGGTATCGCC GCTCCNGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG
  901 ANGAGTNCTT CTGANCGGGA NTCTGGGGTT CGAAATGANC GACCNAGCGA CGCCCAACCT
  961 GNCNNCACGA NNNTTCGATN NCNNCGNCNN CCNTCTATGA AANGNNGGGN TNNNNAANNN
1021 NTTTNNCNGG NNNNNNNNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]