Please review the QC test results indicated by icon below as well as clone information before placing your order.

CoralHue(TM) beta-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)

CoralHue™ β-Actin-targeted monomeric Azami-Green 1 (pActin-mAG1)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.

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Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full)	RDB18314zzk01.seq provided by the depositor
Remarks, protocol and/or map (png)	RDB18314_map.png

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18314_B0H4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert (mAG1 5') Sequence file: RDB18314_B0H4a.seq
>D07039I2_B0H4__2_CMV_Forward_A06_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT GNNGCNNATG GGCGGTANGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC ATTGCCACCA TGGTGAGTGT   121 GATTAAACCA GAGATGAAGA TCAAGCTGTG TATGAGAGGC ACTGTAAACG GGCATAATTT   181 CGTGATTGAA GGAGAAGGAA AAGGAAATCC TTACGAGGGA ACGCAGATTT TAGACCTGAA   241 CGTCACTGAA GGCGCACCTC TGCCTTTCGC TTACGATATC TTGACAACAG TGTTCCAGTA   301 CGGCAACAGG GCATTCACCA AGTACCCAGC AGATATTCAG GACTATTTCA AGCAGACTTT   361 TCCTGAGGGG TATCACTGGG AAAGAAGCAT GACTTATGAA GACCAGGGCA TTTGCACCGC   421 CACAAGCAAC ATAAGCATGA GGGGCGACTG TTTTTTCTAT GACATTCGTT TTGATGGCAC   481 CAACTTTCCT CCCAATGGTC CGGTTATGCA GAAGAAGACT CTTAAATGGG AGCCATCCAC   541 TGAGAAAATG TACGTAGAGG ATGGAGTGCT GAAGGGTGAT GTTAACATGC GCCTGTTGCT   601 TGAAGGAGGT GGCCATTATC GATGTGATTT CAAAACTACT TACAAAGCAA AGAAGGAGGT   661 CCGTTTGCCA GACGCGCACA AAATTGACCA CCGCATTGAG ATTTTGAAGC ATGACAAAGA   721 TTACAACAAG GTCAAGCTCT ATGAGAATGC CGTTGCTCGC TATTCTATGC TGCCGAGTCA   781 GGNCAAGACC GGTAATTCCG CTGACGGCGG CGGANGATCG GGTGGTAGTG GTGGTTCAGG   841 ANGAGGANCG ANCCAAGGAN GNTCCATGGA TGANGATATC GCCGCGCTCG NCGTCGANNA   901 NNGCTCCGGN ATGTGCAAGG NCGGNTNCGC GGGCGANNAT GCCCCCCGGG CCGTCTTCCC   961 CNCCATCGNG GGGCGCCCCN NNNCNNNGGN NTGATGNNGG GNATGGGNCA NANNNANTCC 1021 TATGTGGNNN NNNANNNCNN NNNNNNNN //
Primer: SV40polyA_R2 Region: insert (hActin beta 3') Sequence file: RDB18314_B0H4b.seq
>D07039I2_B0H4__2_SV40polyA_R2_B06_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGTTAA CAACAANNAT TGCATTCNTT TTATGTTTCA    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCTAGAGT CGCGGCCGCT TAGAAGCATT TGCGGTGGAC GATGGAGGGG   181 CCGGACTCGT CATACTCCTG CTTGCTGATC CACATCTGCT GGAAGGTGGA CAGCGAGGCC   241 AGGATGGAGC CGCCGATCCA CACGGAGTAC TTGCGCTCAG GAGGAGCAAT GATCTTGATC   301 TTCATTGTGC TGGGTGCCAG GGCAGTGATC TCCTTCTGCA TCCTGTCGGC AATGCCAGGG   361 TACATGGTGG TGCCGCCAGA CAGCACTGTG TTGGCGTACA GGTCTTTGCG GATGTCCACG   421 TCACACTTCA TGATGGAGTT GAAGGTAGTT TCGTGGATGC CACAGGACTC CATGCCCAGG   481 AAGGAAGGCT GGAAGAGTGC CTCAGGGCAG CGGAACCGCT CATTGCCAAT GGTGATGACC   541 TGGCCGTCAG GCAGCTCGTA GCTCTTCTCC AGGGAGGAGC TGGAAGCAGC CGTGGCCATC   601 TCTTGCTCGA AGTCCAGGGC GACGTAGCAC AGCTTCTCCT TAATGTCACG CACGATTTCC   661 CGCTCGGCCG TGGTGGTGAA GCTGTAGCCG CGCTCGGTGA GGATCTTCAT GAGGTAGTCA   721 GTCAGGTCCC GGCCAGCCAG GTCCAGACGC AGGATGGCAT GGGGGAGGGC ATACCCCTCG   781 TAGATGGGCA CAGTGTGGGT GANCCCGTCA CCGGANTCCA TCACGATGCC AGTGGTACGG   841 CCAGAGGCGT ACAGGGNTAG CACAGCCTNN NTAGCNACGT ACATGGCTGG GGTGNTGAAN   901 GTCTCAAACA TGATCTGGGN CATCTTCTCG CGNTTGGCCT TGGGGTTCNN NGGGCCNCNG   961 NCANCANCAN GGGGNGNTNN TNGGGANNCN CANNCNNCNC NNNNNANNNG NNNNNNNGNC 1021 NNANTTTNNN NNNNNNN //
Primer: mAG_MF1 Region: insert mid(mAG1 3',linker,hActin beta 5') Sequence file: RDB18314_B0H4c.seq
>D07039I2_B0H4__2_mAG_MF1_D06_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTN CNNTAGAGGN NGGAGTGCTG AAGGGTGANG    61 TTAACATGCG CCTGTTGCTT GAAGGAGGTG GCCATTATCG ATGTGATTTC AAAACTACTT   121 ACAAAGCAAA GAAGGAGGTC CGTTTGCCAG ACGCGCACAA AATTGACCAC CGCATTGAGA   181 TTTTGAAGCA TGACAAAGAT TACAACAAGG TCAAGCTCTA TGAGAATGCC GTTGCTCGCT   241 ATTCTATGCT GCCGAGTCAG GCCAAGACCG GTAATTCCGC TGACGGCGGC GGAGGATCGG   301 GTGGTAGTGG TGGTTCAGGA GGAGGATCGA CCCAAGGAGG ATCCATGGAT GATGATATCG   361 CCGCGCTCGT CGTCGACAAC GGCTCCGGCA TGTGCAAGGC CGGCTTCGCG GGCGACGATG   421 CCCCCCGGGC CGTCTTCCCC TCCATCGTGG GGCGCCCCAG GCACCAGGGC GTGATGGTGG   481 GCATGGGTCA GAAGGATTCC TATGTGGGCG ACGAGGCCCA GAGCAAGAGA GGCATCCTCN   541 NCCTGAAGTA CCCCATCGAG CACGGCATCG TCACCAACTG GGACGACATG GAGAAAATCT   601 GGCACCACAC CTTCTACAAT GAGCTGCGTG TGGCTCCCGA GGAGCACCCC GTGCTGCTGA   661 CCGAGGCCCC CCTGAACNNN NNNGCCAACC GCGAGAAGAT GACCCAGATC ATGTTTGAGA   721 CCTTCAACAC CCCAGCCATG TACGTTGCTA TCCAGGNNGN NCNNNNCCTG TNCGCCTCTG   781 GCCGTACCAC TGNNNNCGTG ATGGACTCCG GTGANGGGGT CACNCACNCT GTGCCCATCT   841 ACGANGGGTA TGCCCTCCNN ATGCCATCCN GCGTCTGGAN CTGNCTGGNC NGGANCTGNN   901 CTGANTACNN NNTGAANNNC CTCACCGANC GCNNNTACNG CTNCACCNCN NNNGNCNANC   961 GGGAAATCNN NGNNTGNNAT TAANGANAAN NCTGTGCNNN NGTCNNCCNN GNNCTNNNNN 1021 NCANNNNNNN NGNNCNNNNN TGNNTNNCAG CNNCNTNCCN NNGNANNAAN NNNNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB18314_B0H4d.seq
>D07039I2_B0H4__2_SV40pro_ori_F_C06_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNC GCCTCNGCCT CTGNNCTATT CCNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA   121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG   181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG   241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC   301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC   361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG   421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC   481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA   541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG   601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG   661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG   721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCAGGACA   781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGNGCT GANCGCTTCC   841 TCGNGCTTTA CGGTATCGCC GCTCCNGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG   901 ANGAGTNCTT CTGANCGGGA NTCTGGGGTT CGAAATGANC GACCNAGCGA CGCCCAACCT   961 GNCNNCACGA NNNTTCGATN NCNNCGNCNN CCNTCTATGA AANGNNGGGN TNNNNAANNN 1021 NTTTNNCNGG NNNNNNNNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2021.09.30

GNP_filter3_RDBDEP_html_210618.pl