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CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1) (#RDB18313)

CoralHue™ Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1)

Alternative name AM-V0203M
Clone info. ・For mammalian expression of the Plasma Membrane-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone pPM (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18313zzk01.seq provided by the depositor
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18313 CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18313).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18313_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, mAG1 5'
Sequence file: RDB18313_B0H4a.seq check
>D07039I1_B0H4_1_CMV_Forward_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNTNNNNGC NNNGGGCGGT ANGCGTGTAC GGNGGGNNGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCATTGCC ACCATGGGCT GCATCAAGAG
  121 CAAGCGCAAG GACAACCTGA ACGACGACGG CGTGGACATG GATCCCATGG TGAGTGTGAT
  181 TAAACCAGAG ATGAAGATCA AGCTGTGTAT GAGAGGCACT GTAAACGGGC ATAATTTCGT
  241 GATTGAAGGA GAAGGAAAAG GAAATCCTTA CGAGGGAACG CAGATTTTAG ACCTGAACGT
  301 CACTGAAGGC GCACCTCTGC CTTTCGCTTA CGATATCTTG ACAACAGTGT TCCAGTACGG
  361 CAACAGGGCA TTCACCAAGT ACCCAGCAGA TATTCAGGAC TATTTCAAGC AGACTTTTCC
  421 TGAGGGGTAT CACTGGGAAA GAAGCATGAC TTATGAAGAC CAGGGCATTT GCACCGCCAC
  481 AAGCAACATA AGCATGAGGG GCGACTGTTT TTTCTATGAC ATTCGTTTTG ATGGCACCAA
  541 CTTTCCTCCC AATGGTCCGG TTATGCAGAA GAAGACTCTT AAATGGGAGC CATCCACTGA
  601 GAAAATGTAC GTAGAGGATG GAGTGCTGAA GGGTGATGTT AACATGCGCC TGTTGCTTGA
  661 ANGANGTGGC CATTATCGAT GTGATTTCAA AACTACTTAC AAAGNNNNAA NGANGTCCGT
  721 TTGCCAGACG CGCACAAAAT TGACCACCGC ATTGANATTT TGAAGCATGA CAAAGATTAC
  781 AACAAGGTCN AGCTCTATGA NAATGCCGTT GCTCGCTATT CTATGCTGCC GANNCANGCC
  841 AANTAAGCGG NCGCNANNCT NNNTCATAAT CAGCCNTANC ACATTTGNAG ANGNTTNACT
901 NGCTTTNAAA AAANNNNCNN NNNNNNNCNC NNNNANNNNN NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: mAG1
Sequence file: RDB18313_B0H4b.seq check
>D07039I1_B0H4_1_SV40polyA-R2_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTA ACNACNACAA TTGCATTCNT TTTATGTTTC
   61 NGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
  121 TGGCTGATTA TGATCTAGAG TCGCGGCCGC TTACTTGGCC TGACTCGGCA GCATAGAATA
  181 GCGAGCAACG GCATTCTCAT AGAGCTTGAC CTTGTTGTAA TCTTTGTCAT GCTTCAAAAT
  241 CTCAATGCGG TGGTCAATTT TGTGCGCGTC TGGCAAACGG ACCTCCTTCT TTGCTTTGTA
  301 AGTAGTTTTG AAATCACATC GATAATGGCC ACCTCCTTCA AGCAACAGGC GCATGTTAAC
  361 ATCACCCTTC AGCACTCCAT CCTCTACGTA CATTTTCTCA GTGGATGGCT CCCATTTAAG
  421 AGTCTTCTTC TGCATAACCG GACCATTGGG AGGAAAGTTG GTGCCATCAA AACGAATGTC
  481 ATAGAAAAAA CAGTCGCCCC TCATGCTTAT GTTGCTTGTG GCGGTGCAAA TGCCCTGGTC
  541 TTCATAAGTC ATGCTTCTTT CCCAGTGATA CCCCTCAGGA AAAGTCTGCT TGAAATAGTC
  601 CTGAATATCT GCTGGGTACT TGGTGAATGC CCTGTTGCCG TACTGGAACA CTGTTGTCAA
  661 GATATCGTAA GCGAAAGGCA GAGGTGCGCC TTCAGTGACG TTCAGGTCTA AAATCTGCGT
  721 TCCCTCGTAA GGATTTCCTT TTCCTTCTCC TTCAATCACG AAATTATGCC CGTTTACAGT
  781 GCCTCTCATA CACAGCTTGA TCTTCATCTC TGGTTTAATC ACACTCACCA TGGGNNCCAT
  841 GTCCNCGCCG TCGTCNTTCN NNTGTCCTTG CGCTTGCTCT TGATGCANCC NATGGNGGCA
  901 ATGCTAGCGG ATCTGANNGT TCNCTNAACC NNCTCNGCTN ATATAGACCT CCCNNNGTAN
  961 ACNNNNANNG NCNNTTTGCG NCNNNGGGGN NNANNNGNNN NNNNNTTTTN NNAANTCCNN
1021 NNNNNTTNNN NNNNNANNNA NNNNCNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB18313_B0H4c.seq check
>D07039I1_B0H4_1_SV40pro_ori_F_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNGCCGCCTC NGCCTCTGNN CTANTCNNGA AGTAGTGAGG ANGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AGATCATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
  121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
  181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
  241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
  301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
  361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
  421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
  481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
  541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAANAGCAT
  601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG
  661 GNNCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGNCCGC
  721 TTTTCTGGNN TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGNCATAGCG
  781 TTGGCTACCN GNNATATTGC TGAAGANCTT GNCGGCGAAN GGGCTGANCG CTTCCTCGNG
  841 CTTTACNGNA TCGCCGCTCC NNANTNNCAN CGCATCNCCT TCTATCNNCN TCNNNNNNNN
901 NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]