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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1)

CoralHue™ Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1)

Catalog number RDB18313
Resource name CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1)
Alternative name AM-V0203M
Clone info. ・For mammalian expression of the Plasma Membrane-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone pPM (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Galaxea fascicularis green fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18313 CoralHue(TM) Plasma Membrane-targeted monomeric Azami-Green 1 (pPM-mAG1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB18313zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18313_map.png

            Original reference

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18313_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, mAG1 5'
            Sequence file: RDB18313_B0H4a.seq check
            >D07039I1_B0H4_1_CMV_Forward_D04_10_ABI24.ab1
                1 NNNNNNNNNN NNNTNNNNGC NNNGGGCGGT ANGCGTGTAC GGNGGGNNGT CTATATAAGC
               61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCATTGCC ACCATGGGCT GCATCAAGAG
              121 CAAGCGCAAG GACAACCTGA ACGACGACGG CGTGGACATG GATCCCATGG TGAGTGTGAT
              181 TAAACCAGAG ATGAAGATCA AGCTGTGTAT GAGAGGCACT GTAAACGGGC ATAATTTCGT
              241 GATTGAAGGA GAAGGAAAAG GAAATCCTTA CGAGGGAACG CAGATTTTAG ACCTGAACGT
              301 CACTGAAGGC GCACCTCTGC CTTTCGCTTA CGATATCTTG ACAACAGTGT TCCAGTACGG
              361 CAACAGGGCA TTCACCAAGT ACCCAGCAGA TATTCAGGAC TATTTCAAGC AGACTTTTCC
              421 TGAGGGGTAT CACTGGGAAA GAAGCATGAC TTATGAAGAC CAGGGCATTT GCACCGCCAC
              481 AAGCAACATA AGCATGAGGG GCGACTGTTT TTTCTATGAC ATTCGTTTTG ATGGCACCAA
              541 CTTTCCTCCC AATGGTCCGG TTATGCAGAA GAAGACTCTT AAATGGGAGC CATCCACTGA
              601 GAAAATGTAC GTAGAGGATG GAGTGCTGAA GGGTGATGTT AACATGCGCC TGTTGCTTGA
              661 ANGANGTGGC CATTATCGAT GTGATTTCAA AACTACTTAC AAAGNNNNAA NGANGTCCGT
              721 TTGCCAGACG CGCACAAAAT TGACCACCGC ATTGANATTT TGAAGCATGA CAAAGATTAC
              781 AACAAGGTCN AGCTCTATGA NAATGCCGTT GCTCGCTATT CTATGCTGCC GANNCANGCC
              841 AANTAAGCGG NCGCNANNCT NNNTCATAAT CAGCCNTANC ACATTTGNAG ANGNTTNACT
            901 NGCTTTNAAA AAANNNNCNN NNNNNNNCNC NNNNANNNNN NNNNN
            //
            Primer: SV40polyA_R2
            Region: mAG1
            Sequence file: RDB18313_B0H4b.seq check
            >D07039I1_B0H4_1_SV40polyA-R2_F08_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTTA ACNACNACAA TTGCATTCNT TTTATGTTTC
               61 NGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
              121 TGGCTGATTA TGATCTAGAG TCGCGGCCGC TTACTTGGCC TGACTCGGCA GCATAGAATA
              181 GCGAGCAACG GCATTCTCAT AGAGCTTGAC CTTGTTGTAA TCTTTGTCAT GCTTCAAAAT
              241 CTCAATGCGG TGGTCAATTT TGTGCGCGTC TGGCAAACGG ACCTCCTTCT TTGCTTTGTA
              301 AGTAGTTTTG AAATCACATC GATAATGGCC ACCTCCTTCA AGCAACAGGC GCATGTTAAC
              361 ATCACCCTTC AGCACTCCAT CCTCTACGTA CATTTTCTCA GTGGATGGCT CCCATTTAAG
              421 AGTCTTCTTC TGCATAACCG GACCATTGGG AGGAAAGTTG GTGCCATCAA AACGAATGTC
              481 ATAGAAAAAA CAGTCGCCCC TCATGCTTAT GTTGCTTGTG GCGGTGCAAA TGCCCTGGTC
              541 TTCATAAGTC ATGCTTCTTT CCCAGTGATA CCCCTCAGGA AAAGTCTGCT TGAAATAGTC
              601 CTGAATATCT GCTGGGTACT TGGTGAATGC CCTGTTGCCG TACTGGAACA CTGTTGTCAA
              661 GATATCGTAA GCGAAAGGCA GAGGTGCGCC TTCAGTGACG TTCAGGTCTA AAATCTGCGT
              721 TCCCTCGTAA GGATTTCCTT TTCCTTCTCC TTCAATCACG AAATTATGCC CGTTTACAGT
              781 GCCTCTCATA CACAGCTTGA TCTTCATCTC TGGTTTAATC ACACTCACCA TGGGNNCCAT
              841 GTCCNCGCCG TCGTCNTTCN NNTGTCCTTG CGCTTGCTCT TGATGCANCC NATGGNGGCA
              901 ATGCTAGCGG ATCTGANNGT TCNCTNAACC NNCTCNGCTN ATATAGACCT CCCNNNGTAN
              961 ACNNNNANNG NCNNTTTGCG NCNNNGGGGN NNANNNGNNN NNNNNTTTTN NNAANTCCNN
            1021 NNNNNTTNNN NNNNNANNNA NNNNCNNNNN NNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR
            Sequence file: RDB18313_B0H4c.seq check
            >D07039I1_B0H4_1_SV40pro_ori_F_D06_12_ABI24.ab1
                1 NNNNNNNNNN NNGCCGCCTC NGCCTCTGNN CTANTCNNGA AGTAGTGAGG ANGCTTTTTT
               61 GGAGGCCTAG GCTTTTGCAA AGATCATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT
              121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
              181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
              241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
              301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
              361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
              421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
              481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
              541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAANAGCAT
              601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG
              661 GNNCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGNCCGC
              721 TTTTCTGGNN TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGNCATAGCG
              781 TTGGCTACCN GNNATATTGC TGAAGANCTT GNCGGCGAAN GGGCTGANCG CTTCCTCGNG
              841 CTTTACNGNA TCGCCGCTCC NNANTNNCAN CGCATCNCCT TCTATCNNCN TCNNNNNNNN
            901 NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            2022.05.11

            GNP_filter3_RDBDEP_html_220427.pl