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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1)

CoralHue™ Mitochondria-targeted monomeric Azami-Green (pMT-mAG1)

Catalog number RDB18311
Resource name CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1)
Alternative name AM-V0201M
Clone info. ・For mammalian expression of the Mitochondria-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505

Vector backbone pMT (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Galaxea fascicularis green fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18311 CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1) DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB18311zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18311_map.png

            Original reference

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18311_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, insert 5'(mAG1 5')
            Sequence file: RDB18311_B0H4a.seq check
            >D07039H8_B0H4_2_CMV_Forward_C04_07_ABI24.ab1
                1 NNNNNNNNNC CNTTNNNGCN NTGGGCGGTA NGCGTGTACG GTGGGNNGTC TATATAAGCA
               61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCATTGCCA CCATGCTGAG CCTGCGCCAG
              121 AGTATCCGCT TCTTCAAGCC CGCCACCAGG ACTCTGTGCA GTTCCAGGGC GGCCGCGGGG
              181 ACAATGGTGA GTGTGATTAA ACCAGAGATG AAGATCAAGC TGTGTATGAG AGGCACTGTA
              241 AACGGGCATA ATTTCGTGAT TGAAGGAGAA GGAAAAGGAA ATCCTTACGA GGGAACGCAG
              301 ATTTTAGACC TGAACGTCAC TGAAGGCGCA CCTCTGCCTT TCGCTTACGA TATCTTGACA
              361 ACAGTGTTCC AGTACGGCAA CAGGGCATTC ACCAAGTACC CAGCAGATAT TCAGGACTAT
              421 TTCAAGCAGA CTTTTCCTGA GGGGTATCAC TGGGAAAGAA GCATGACTTA TGAAGACCAG
              481 GGCATTTGCA CCGCCACAAG CAACATAAGC ATGAGGGGCG ACTGTTTTTT CTATGACATT
              541 CGTTTTGATG GCACCAACTT TCCTCCCAAT GGTCCGGTTA TGCAGAAGAA GACTCTTAAA
              601 TGGGAGCCAT CCACTGAGAA AATGTACGTA GAGGNNGGAG TGCTGAAGGG TGATGTTAAC
              661 ATGCGCCTGT TGCTTGAANG ANGTGGCCAT TATCGATGTG ATTTCAAAAC TACTTACAAA
              721 GCANAGAANG ANGTCCGTTT GCCAGACGCG CACAAAATTG ACCACCGCAT TGAGATTTTG
              781 AAGCATGACA AAGATTACAA CAAGGTCAAG CTCTATGANA ATGCCGTTGC TCGCTANTCT
              841 ATGCTGCCNA NNCANGNCNA GTAATCNANA NGGGGGCCGC NANNCTANNT CATANTCAGC
              901 NNTANNNCNN TTGNANNNGN TTTNANTNNN NTNNAAAAAN NNCNNNCNNN NNCNNNCNNN
            961 NNNNNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'(mAG1 3')
            Sequence file: RDB18311_B0H4b.seq check
            >D07039H8_B0H4_2_SV40polyA-R2_C05_08_ABI24.ab1
                1 NNNNNNNNCN NNNNNNNAGT TAACAACAAC NATTGNATTC ATTTTATGTT TCAGGTTCAG
               61 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
              121 TATGATCTAG AGTCGCGGCC CCCTCTAGAT TACTTGGCCT GACTCGGCAG CATAGAATAG
              181 CGAGCAACGG CATTCTCATA GAGCTTGACC TTGTTGTAAT CTTTGTCATG CTTCAAAATC
              241 TCAATGCGGT GGTCAATTTT GTGCGCGTCT GGCAAACGGA CCTCCTTCTT TGCTTTGTAA
              301 GTAGTTTTGA AATCACATCG ATAATGGCCA CCTCCTTCAA GCAACAGGCG CATGTTAACA
              361 TCACCCTTCA GCACTCCATC CTCTACGTAC ATTTTCTCAG TGGATGGCTC CCATTTAAGA
              421 GTCTTCTTCT GCATAACCGG ACCATTGGGA GGAAAGTTGG TGCCATCAAA ACGAATGTCA
              481 TAGAAAAAAC AGTCGCCCCT CATGCTTATG TTGCTTGTGG CGGTGCAAAT GCCCTGGTCT
              541 TCATAAGTCA TGCTTCTTTC CCAGTGATAC CCCTCAGGAA AAGTCTGCTT GAAATAGTCC
              601 TGAATATCTG CTGGGTACTT GGTGAATGCC CTGTTGCCGT ACTGGAACAC TGTTGTCAAG
              661 ATATCGTAAG CGAAAGGCAG ANGTGCGCCT TCAGTGNCGT TCANGTCTAA AATCTGCGTT
              721 CCCTCGTAAG GNTTTCCTTT TCCTTCTCCT TCAATCACGA AATTATGCCC GTTTACAGTG
              781 CCTCTCATAC ACAGCTTGAT CTTCATCTCT GGTTNAATCA CACTCACCAT TGTCCCCGCG
              841 GCCNNNNNGN AACTGCNCAN ANNNCNGGNN GCGGGCTNNA ANAANCGNNN ANTCNNGNNN
            901 NNNCTCANCA NGNNNNNNNN NN
            //
            Primer: SV40pro_ori_F
            Region: NeoR_KanR
            Sequence file: RDB18311_B0H4c.seq check
            >D07039H8_B0H4_2_SV40pro_ori_F_C06_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNGCCTC NGCCTCTGNN CTANTCNNGA AGTAGTGAGG NNGCTTTTTT
               61 GGAGGCCTAG GCTTTTGCAA AGATCATCAA GAGACAGGAT GAGGNTCGTT TCGCATGATT
              121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
              181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
              241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
              301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
              361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
              421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
              481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
              541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
              601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN
              661 GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA NNATGGCCGC
              721 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG
              781 TTGGCTACNC GNNNTATTGC TGAAGANCTT GGCGGCGAAN GGGNCTGANC GCTTCCTCGN
              841 GCTTTACNGT ATCGCCGCTC CCNANNCGCA NCGCATCGCC TTCNNTCGCN NTCTNNNNNN
            901 NNNCTTCNNN NCGGNNNNNN NNGNNNNCNA ANNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206.

            2021.11.05

            GNP_filter3_RDBDEP_html_211029.pl