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CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1) (#RDB18311)

CoralHue™ Mitochondria-targeted monomeric Azami-Green (pMT-mAG1)

Alternative name AM-V0201M
Clone info. ・For mammalian expression of the Mitochondria-targeted fluorescent protein.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone pMT (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18311zzk01.seq provided by the depositor
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18311 CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) Mitochondria-targeted monomeric Azami-Green (pMT-mAG1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18311).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18311_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'(mAG1 5')
Sequence file: RDB18311_B0H4a.seq check
>D07039H8_B0H4_2_CMV_Forward_C04_07_ABI24.ab1
    1 NNNNNNNNNC CNTTNNNGCN NTGGGCGGTA NGCGTGTACG GTGGGNNGTC TATATAAGCA
   61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCATTGCCA CCATGCTGAG CCTGCGCCAG
  121 AGTATCCGCT TCTTCAAGCC CGCCACCAGG ACTCTGTGCA GTTCCAGGGC GGCCGCGGGG
  181 ACAATGGTGA GTGTGATTAA ACCAGAGATG AAGATCAAGC TGTGTATGAG AGGCACTGTA
  241 AACGGGCATA ATTTCGTGAT TGAAGGAGAA GGAAAAGGAA ATCCTTACGA GGGAACGCAG
  301 ATTTTAGACC TGAACGTCAC TGAAGGCGCA CCTCTGCCTT TCGCTTACGA TATCTTGACA
  361 ACAGTGTTCC AGTACGGCAA CAGGGCATTC ACCAAGTACC CAGCAGATAT TCAGGACTAT
  421 TTCAAGCAGA CTTTTCCTGA GGGGTATCAC TGGGAAAGAA GCATGACTTA TGAAGACCAG
  481 GGCATTTGCA CCGCCACAAG CAACATAAGC ATGAGGGGCG ACTGTTTTTT CTATGACATT
  541 CGTTTTGATG GCACCAACTT TCCTCCCAAT GGTCCGGTTA TGCAGAAGAA GACTCTTAAA
  601 TGGGAGCCAT CCACTGAGAA AATGTACGTA GAGGNNGGAG TGCTGAAGGG TGATGTTAAC
  661 ATGCGCCTGT TGCTTGAANG ANGTGGCCAT TATCGATGTG ATTTCAAAAC TACTTACAAA
  721 GCANAGAANG ANGTCCGTTT GCCAGACGCG CACAAAATTG ACCACCGCAT TGAGATTTTG
  781 AAGCATGACA AAGATTACAA CAAGGTCAAG CTCTATGANA ATGCCGTTGC TCGCTANTCT
  841 ATGCTGCCNA NNCANGNCNA GTAATCNANA NGGGGGCCGC NANNCTANNT CATANTCAGC
  901 NNTANNNCNN TTGNANNNGN TTTNANTNNN NTNNAAAAAN NNCNNNCNNN NNCNNNCNNN
961 NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'(mAG1 3')
Sequence file: RDB18311_B0H4b.seq check
>D07039H8_B0H4_2_SV40polyA-R2_C05_08_ABI24.ab1
    1 NNNNNNNNCN NNNNNNNAGT TAACAACAAC NATTGNATTC ATTTTATGTT TCAGGTTCAG
   61 GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT
  121 TATGATCTAG AGTCGCGGCC CCCTCTAGAT TACTTGGCCT GACTCGGCAG CATAGAATAG
  181 CGAGCAACGG CATTCTCATA GAGCTTGACC TTGTTGTAAT CTTTGTCATG CTTCAAAATC
  241 TCAATGCGGT GGTCAATTTT GTGCGCGTCT GGCAAACGGA CCTCCTTCTT TGCTTTGTAA
  301 GTAGTTTTGA AATCACATCG ATAATGGCCA CCTCCTTCAA GCAACAGGCG CATGTTAACA
  361 TCACCCTTCA GCACTCCATC CTCTACGTAC ATTTTCTCAG TGGATGGCTC CCATTTAAGA
  421 GTCTTCTTCT GCATAACCGG ACCATTGGGA GGAAAGTTGG TGCCATCAAA ACGAATGTCA
  481 TAGAAAAAAC AGTCGCCCCT CATGCTTATG TTGCTTGTGG CGGTGCAAAT GCCCTGGTCT
  541 TCATAAGTCA TGCTTCTTTC CCAGTGATAC CCCTCAGGAA AAGTCTGCTT GAAATAGTCC
  601 TGAATATCTG CTGGGTACTT GGTGAATGCC CTGTTGCCGT ACTGGAACAC TGTTGTCAAG
  661 ATATCGTAAG CGAAAGGCAG ANGTGCGCCT TCAGTGNCGT TCANGTCTAA AATCTGCGTT
  721 CCCTCGTAAG GNTTTCCTTT TCCTTCTCCT TCAATCACGA AATTATGCCC GTTTACAGTG
  781 CCTCTCATAC ACAGCTTGAT CTTCATCTCT GGTTNAATCA CACTCACCAT TGTCCCCGCG
  841 GCCNNNNNGN AACTGCNCAN ANNNCNGGNN GCGGGCTNNA ANAANCGNNN ANTCNNGNNN
901 NNNCTCANCA NGNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: NeoR_KanR
Sequence file: RDB18311_B0H4c.seq check
>D07039H8_B0H4_2_SV40pro_ori_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNGCCTC NGCCTCTGNN CTANTCNNGA AGTAGTGAGG NNGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AGATCATCAA GAGACAGGAT GAGGNTCGTT TCGCATGATT
  121 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT
  181 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG
  241 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC
  301 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC
  361 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC
  421 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG
  481 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG
  541 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT
  601 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAN
  661 GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA NNATGGCCGC
  721 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA NGACATAGCG
  781 TTGGCTACNC GNNNTATTGC TGAAGANCTT GGCGGCGAAN GGGNCTGANC GCTTCCTCGN
  841 GCTTTACNGT ATCGCCGCTC CCNANNCGCA NCGCATCGCC TTCNNTCGCN NTCTNNNNNN
901 NNNCTTCNNN NCGGNNNNNN NNGNNNNCNA ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]