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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1) (#RDB18295)

CoralHue™ humanized dimeric Keima570 (phdKeima570-S1)

Alternative name AM-V0124M
Clone info. ・For subcloning.
・The codons are humanized.
・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/570
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18295zzk01.seq provided by the depositor
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18295 CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18295).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18295_B0Krp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18295_B0Kra.seq check
>D07039G1_B0Kr_7_lacIpro-B_F_E05_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN CNNCGNNTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
  121 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
  181 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
  241 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAGCT CGGTACCCGG
  301 GGATCCCCAC CATGGTGAGC GTGATCGCCA AGCAGATGAC CTACAAGGTG TACATGTCCG
  361 GCACCGTGAA CGGCCACTAC TTCGAGGTGG AGGGCGACGG CAAGGGCAAG CCCTACGAGG
  421 GCGAGCAGAC CGTGAAGCTG ACCGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA
  481 TCCTGTCCCC CCTGATGTGC TACGGCAGCA TCCCCTTCAC CAAGTACCCC GAGGACATCC
  541 CCGACTACGT GAAGCAGAGC TTCCCCGAGG GCTACACCTG GGAGAGGACC ATGAACTTCG
  601 AGGACGGCGC CGTGTGCACC GTGAGCAACG ACTCCAGCAT CCAGGGCAAC TGCTTCATCT
  661 ACAACGTGAA GATCAGCGGC ACCAACTTCC CCCCCAACGG CCCCGTGATG CAGAAGAAGA
  721 NCCAGGGCTG GGAGCCCAGC ACCGAGAGGC TGTTCGCCAG GGACGGAATG CTGATCGGCA
  781 ACGACTACAT GGCCCTGAAG CTGGAGGGCG GCGGCCACTA CCTGTGCGAG TTCAAGTCCN
  841 CCTACNANGN CAAGAAGCCC GTGAGGANGC CCGGNTACCA CTACNTCGAC NGGAAGCTGG
  901 ANNTGACCNG CCNCANCNGG GANTACNCCT CCGTGGANNN NNGNGAGATC GCCNTCGCCN
  961 GGCACTCCCT GNTGGNCTAA CTGNNNGNNT GNNANCTTGG NACNNGNNNG NCNTTTTNNA
1021 ANNNCNNGNN NGGGNAAAAN CNNNNNNNNN N
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 3'
Sequence file: RDB18295_B0Krb.seq check
>D07039G1_B0Kr_7_pUC-M13_-100_F05_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGGNNNNNC CNGGGTTTTC CCNGTCNCGA CGTTGTAAAA
   61 CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGTTAGCCCA GCAGGGAGTG CCTGGCGATG
  121 GCGATCTCGC ACTGCTCCAC GGAGGTGTAG TCCCTGTTGT GGCTGGTCAC GTCCAGCTTC
  181 CTGTCGATGT AGTGGTAGCC GGGCATCCTC ACGGGCTTCT TGGCCTTGTA GGTGGACTTG
  241 AACTCGCACA GGTAGTGGCC GCCGCCCTCC AGCTTCAGGG CCATGTAGTC GTTGCCGATC
  301 AGCATTCCGT CCCTGGCGAA CAGCCTCTCG GTGCTGGGCT CCCAGCCCTG GGTCTTCTTC
  361 TGCATCACGG GGCCGTTGGG GGGGAAGTTG GTGCCGCTGA TCTTCACGTT GTAGATGAAG
  421 CAGTTGCCCT GGATGCTGGA GTCGTTGCTC ACGGTGCACA CGGCGCCGTC CTCGAAGTTC
  481 ATGGTCCTCT CCCAGGTGTA GCCCTCGGGG AAGCTCTGCT TCACGTAGTC GGGGATGTCC
  541 TCGGGGTACT TGGTGAAGGG GATGCTGCCG TAGCACATCA GGGGGGACAG GATGTCCCAG
  601 GCGAAGGGCA GGGGGCCGCC CTTGGTCACG GTCAGCTTCA CGGTCTGCTC GCCCTCGTAG
  661 GGCTTGCCCT TGCCGTCGCC CTCCACCTCG AAGTAGTGGC CGTTCACGGT GCCGGACATG
  721 TACACCTTGT AGGTCATCTG CTTGGCGATC ACGCTCNCCA TGGTGGGGAT CCCCGGGTAC
  781 CGAGCTCGAA TTCGTAATCA TGGTCNTAGC TGTTTCCTGT GTGAAATTGN TATCCGCTCA
  841 CAATTCCNCA CNACATACNA NCCGGAANNC NTNAAGTGTA AAGCCTGGGG TGNCNNATGA
  901 GTGANCNNAC TCANNNNAAT TGCGTTGCNN TNNCTGCCCG CTTTNCNNNC GGGAAACCTG
  961 TCNNGNCAGN TGCNTNNATG NATCGGNNNA CGCGCNGGGA NANGNGGNTT NNGTANTNGN
 1021 NNNTCNTCCN CTTNCTNNCT CNCTGANTCN CTGNGNTNNG NNNNTTCNGN TGNNGCNNNC
1081 NNNNNNNNNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]