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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)

CoralHue™ humanized dimeric Keima570 (phdKeima570-S1)

Catalog number RDB18295
Resource name CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)
Alternative name AM-V0124M
Clone info. ・For subcloning.
・The codons are humanized.
・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/570
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18295zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18295_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18295 CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18295_B0Krp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18295_B0Kra.seq check
            >D07039G1_B0Kr_7_lacIpro-B_F_E05_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNNCGNNTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA
               61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
              121 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
              181 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
              241 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAGCT CGGTACCCGG
              301 GGATCCCCAC CATGGTGAGC GTGATCGCCA AGCAGATGAC CTACAAGGTG TACATGTCCG
              361 GCACCGTGAA CGGCCACTAC TTCGAGGTGG AGGGCGACGG CAAGGGCAAG CCCTACGAGG
              421 GCGAGCAGAC CGTGAAGCTG ACCGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA
              481 TCCTGTCCCC CCTGATGTGC TACGGCAGCA TCCCCTTCAC CAAGTACCCC GAGGACATCC
              541 CCGACTACGT GAAGCAGAGC TTCCCCGAGG GCTACACCTG GGAGAGGACC ATGAACTTCG
              601 AGGACGGCGC CGTGTGCACC GTGAGCAACG ACTCCAGCAT CCAGGGCAAC TGCTTCATCT
              661 ACAACGTGAA GATCAGCGGC ACCAACTTCC CCCCCAACGG CCCCGTGATG CAGAAGAAGA
              721 NCCAGGGCTG GGAGCCCAGC ACCGAGAGGC TGTTCGCCAG GGACGGAATG CTGATCGGCA
              781 ACGACTACAT GGCCCTGAAG CTGGAGGGCG GCGGCCACTA CCTGTGCGAG TTCAAGTCCN
              841 CCTACNANGN CAAGAAGCCC GTGAGGANGC CCGGNTACCA CTACNTCGAC NGGAAGCTGG
              901 ANNTGACCNG CCNCANCNGG GANTACNCCT CCGTGGANNN NNGNGAGATC GCCNTCGCCN
              961 GGCACTCCCT GNTGGNCTAA CTGNNNGNNT GNNANCTTGG NACNNGNNNG NCNTTTTNNA
            1021 ANNNCNNGNN NGGGNAAAAN CNNNNNNNNN N
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18295_B0Krb.seq check
            >D07039G1_B0Kr_7_pUC-M13_-100_F05_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGGNNNNNC CNGGGTTTTC CCNGTCNCGA CGTTGTAAAA
               61 CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGTTAGCCCA GCAGGGAGTG CCTGGCGATG
              121 GCGATCTCGC ACTGCTCCAC GGAGGTGTAG TCCCTGTTGT GGCTGGTCAC GTCCAGCTTC
              181 CTGTCGATGT AGTGGTAGCC GGGCATCCTC ACGGGCTTCT TGGCCTTGTA GGTGGACTTG
              241 AACTCGCACA GGTAGTGGCC GCCGCCCTCC AGCTTCAGGG CCATGTAGTC GTTGCCGATC
              301 AGCATTCCGT CCCTGGCGAA CAGCCTCTCG GTGCTGGGCT CCCAGCCCTG GGTCTTCTTC
              361 TGCATCACGG GGCCGTTGGG GGGGAAGTTG GTGCCGCTGA TCTTCACGTT GTAGATGAAG
              421 CAGTTGCCCT GGATGCTGGA GTCGTTGCTC ACGGTGCACA CGGCGCCGTC CTCGAAGTTC
              481 ATGGTCCTCT CCCAGGTGTA GCCCTCGGGG AAGCTCTGCT TCACGTAGTC GGGGATGTCC
              541 TCGGGGTACT TGGTGAAGGG GATGCTGCCG TAGCACATCA GGGGGGACAG GATGTCCCAG
              601 GCGAAGGGCA GGGGGCCGCC CTTGGTCACG GTCAGCTTCA CGGTCTGCTC GCCCTCGTAG
              661 GGCTTGCCCT TGCCGTCGCC CTCCACCTCG AAGTAGTGGC CGTTCACGGT GCCGGACATG
              721 TACACCTTGT AGGTCATCTG CTTGGCGATC ACGCTCNCCA TGGTGGGGAT CCCCGGGTAC
              781 CGAGCTCGAA TTCGTAATCA TGGTCNTAGC TGTTTCCTGT GTGAAATTGN TATCCGCTCA
              841 CAATTCCNCA CNACATACNA NCCGGAANNC NTNAAGTGTA AAGCCTGGGG TGNCNNATGA
              901 GTGANCNNAC TCANNNNAAT TGCGTTGCNN TNNCTGCCCG CTTTNCNNNC GGGAAACCTG
              961 TCNNGNCAGN TGCNTNNATG NATCGGNNNA CGCGCNGGGA NANGNGGNTT NNGTANTNGN
             1021 NNNTCNTCCN CTTNCTNNCT CNCTGANTCN CTGNGNTNNG NNNNTTCNGN TGNNGCNNNC
            1081 NNNNNNNNNT NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl