RDB18295 - CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)

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CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)

CoralHue™ humanized dimeric Keima570 (phdKeima570-S1)

Catalog number	RDB18295
Resource name	CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)
Alternative name	AM-V0124M
Clone info.	・For subcloning. ・The codons are humanized. ・Dimeric Keima570 exhibits an extremely large Stokes shift(130 nm) and is stable under usual aerobic conditions. ・Ex/Em(nm): 440/570
Vector backbone	S1 (plasmid)
Size of vector backbone	2.7 kb
Selectable markers	Ampicillin
Gene/insert name	Montipora. sp red fluorescent protein cDNA
Depositor\|Developer	MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., \| Nakajo, Tomohiro \| Karasawa, Satoshi \|

Other clones in our bank

External Database
Montipora. sp red fluorescent protein

Reference sequence

Sequence (full)	RDB18295zzk01.seq provided by the depositor
Remarks, protocol and/or map (png)	RDB18295_map.png

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
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提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18295	CoralHue(TM) humanized dimeric Keima570 (phdKeima570-S1)	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content	Fluorescent protein resources (English text)
Featured content	Fluorescent protein resources (Japanese text)
Featured content	CoralHue™ Fluorescent Protein (English text)
Featured content	CoralHue™ Fluorescent Protein (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18295_B0Krp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18295_B0Kra.seq
>D07039G1_B0Kr_7_lacIpro-B_F_E05_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN CNNCGNNTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA 61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT 121 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG 181 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 241 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAGCT CGGTACCCGG 301 GGATCCCCAC CATGGTGAGC GTGATCGCCA AGCAGATGAC CTACAAGGTG TACATGTCCG 361 GCACCGTGAA CGGCCACTAC TTCGAGGTGG AGGGCGACGG CAAGGGCAAG CCCTACGAGG 421 GCGAGCAGAC CGTGAAGCTG ACCGTGACCA AGGGCGGCCC CCTGCCCTTC GCCTGGGACA 481 TCCTGTCCCC CCTGATGTGC TACGGCAGCA TCCCCTTCAC CAAGTACCCC GAGGACATCC 541 CCGACTACGT GAAGCAGAGC TTCCCCGAGG GCTACACCTG GGAGAGGACC ATGAACTTCG 601 AGGACGGCGC CGTGTGCACC GTGAGCAACG ACTCCAGCAT CCAGGGCAAC TGCTTCATCT 661 ACAACGTGAA GATCAGCGGC ACCAACTTCC CCCCCAACGG CCCCGTGATG CAGAAGAAGA 721 NCCAGGGCTG GGAGCCCAGC ACCGAGAGGC TGTTCGCCAG GGACGGAATG CTGATCGGCA 781 ACGACTACAT GGCCCTGAAG CTGGAGGGCG GCGGCCACTA CCTGTGCGAG TTCAAGTCCN 841 CCTACNANGN CAAGAAGCCC GTGAGGANGC CCGGNTACCA CTACNTCGAC NGGAAGCTGG 901 ANNTGACCNG CCNCANCNGG GANTACNCCT CCGTGGANNN NNGNGAGATC GCCNTCGCCN 961 GGCACTCCCT GNTGGNCTAA CTGNNNGNNT GNNANCTTGG NACNNGNNNG NCNTTTTNNA 1021 ANNNCNNGNN NGGGNAAAAN CNNNNNNNNN N //
Primer: pUC-M13_-100 Region: MCS, insert 3' Sequence file: RDB18295_B0Krb.seq
>D07039G1_B0Kr_7_pUC-M13_-100_F05_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGGNNNNNC CNGGGTTTTC CCNGTCNCGA CGTTGTAAAA 61 CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGTTAGCCCA GCAGGGAGTG CCTGGCGATG 121 GCGATCTCGC ACTGCTCCAC GGAGGTGTAG TCCCTGTTGT GGCTGGTCAC GTCCAGCTTC 181 CTGTCGATGT AGTGGTAGCC GGGCATCCTC ACGGGCTTCT TGGCCTTGTA GGTGGACTTG 241 AACTCGCACA GGTAGTGGCC GCCGCCCTCC AGCTTCAGGG CCATGTAGTC GTTGCCGATC 301 AGCATTCCGT CCCTGGCGAA CAGCCTCTCG GTGCTGGGCT CCCAGCCCTG GGTCTTCTTC 361 TGCATCACGG GGCCGTTGGG GGGGAAGTTG GTGCCGCTGA TCTTCACGTT GTAGATGAAG 421 CAGTTGCCCT GGATGCTGGA GTCGTTGCTC ACGGTGCACA CGGCGCCGTC CTCGAAGTTC 481 ATGGTCCTCT CCCAGGTGTA GCCCTCGGGG AAGCTCTGCT TCACGTAGTC GGGGATGTCC 541 TCGGGGTACT TGGTGAAGGG GATGCTGCCG TAGCACATCA GGGGGGACAG GATGTCCCAG 601 GCGAAGGGCA GGGGGCCGCC CTTGGTCACG GTCAGCTTCA CGGTCTGCTC GCCCTCGTAG 661 GGCTTGCCCT TGCCGTCGCC CTCCACCTCG AAGTAGTGGC CGTTCACGGT GCCGGACATG 721 TACACCTTGT AGGTCATCTG CTTGGCGATC ACGCTCNCCA TGGTGGGGAT CCCCGGGTAC 781 CGAGCTCGAA TTCGTAATCA TGGTCNTAGC TGTTTCCTGT GTGAAATTGN TATCCGCTCA 841 CAATTCCNCA CNACATACNA NCCGGAANNC NTNAAGTGTA AAGCCTGGGG TGNCNNATGA 901 GTGANCNNAC TCANNNNAAT TGCGTTGCNN TNNCTGCCCG CTTTNCNNNC GGGAAACCTG 961 TCNNGNCAGN TGCNTNNATG NATCGGNNNA CGCGCNGGGA NANGNGGNTT NNGTANTNGN 1021 NNNTCNTCCN CTTNCTNNCT CNCTGANTCN CTGNGNTNNG NNNNTTCNGN TGNNGCNNNC 1081 NNNNNNNNNT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl