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CoralHue(TM) monomeric Midoriishi-Cyan 1 (pmMiCy1-S1)

CoralHue™ monomeric Midoriishi-Cyan 1 (pmMiCy1-S1)

Catalog number RDB18289
Resource name CoralHue(TM) monomeric Midoriishi-Cyan 1 (pmMiCy1-S1)
Alternative name AM-V0111M
Clone info. ・For subcloning.
・Monomeric Midoriishi-Cyan1 is Cyan fluorescent protein.
・Ex/Em(nm): 472/495

Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Acropara sp. cyan fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18289 CoralHue(TM) monomeric Midoriishi-Cyan 1 (pmMiCy1-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB18289zzk02.seq provided by the depositor
Remarks, protocol and/or map (png) RDB18289_map.png

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18289_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18289_B0H4a.seq check
>D07039F4_B0H4_13_lacIpro-B_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN CGNNNCNGTG AGCGAGGAAG CGGAAGANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
  121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
  181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA
  301 TCCCCACCAT GGTGTCTTAT TCAAAGCAAG GCATCGCACA AGAAATGCGG ACGAAATACC
  361 GTATGGAAGG CAGTGTCAAT GGCCATGAGT TCACGATCGA AGGTGTAGGA ACTGGAAACC
  421 CTTACGAAGG GAAACAGACG TCCGAATTAG TGATCATCAA GCCTAAGGGA AAACCCCTTC
  481 CATTCTCCTT TGACATACTG TCAACAGTCT TTCAATATGG AAACAGATGC TTCACAAAGT
  541 ACCCTGCCGA CATGCCTGAC TATTTCAAGC AAGCATTCCC AGATGGAATG TCATATGAAA
  601 GGTCATTTCT ATTTGAGGAT GGAGGAGTTG CTACAGCCAG CTGGAGCATT CGTCTCGAAG
  661 GAAATTGCTT CATCCACAAT TCCATCTATC ATGGCACAAA CTTTCCCGCT GATGGACCCG
  721 TAATGAAGAA GCAGACAATT GGCTGGGATA AGTCCTCCGA AAAAATGAGT GTGGCTAAAG
  781 AGGTGCTAAG ANGTGATGTG ACTCAGTTTC TTCTGCTCGA ANGANGTGGT TACCAGANAT
  841 GCCAGTTACA CTCCACTTAC AAAACAGANA ANCCAGTCGC AANGNCCCCC NANNCATGTC
  901 GNANAACATC NAATTGNNAN GANCGNNNTN NGNNNAANNG CNAANNNNTT CANNGNCAAN
961 CNGGNNNNNN NTGCNNANGN NNNNNNNNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18289_B0H4b.seq check
>D07039F4_B0H4_13_pUC-M13_-100_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGG NNNNNNNNGG GTTTTCCCNG TCNCGACGTT GTAAAACGAC
   61 GGCCAGTGCC AAGCTTGCAT GCCTGCAGTT ATTTAACCTT CAAAGGGTTA ACATGAGCCT
  121 CAGCATGTTC TTCCAGCTTG ACCTTGAAGC CTTTTGCAGT TTGGCCAAGG TCGGTCCTCA
  181 CAATTTGATG TTCTACGACA TGACTCGGGG GCATTGCGAC TGGCTTCTCT GTTTTGTAAG
  241 TGGAGTGTAA CTGGCATCTC TGGTAACCAC CTCCTTCGAG CAGAAGAAAC TGAGTCACAT
  301 CACCTCTTAG CACCTCTTTA GCCACACTCA TTTTTTCGGA GGACTTATCC CAGCCAATTG
  361 TCTGCTTCTT CATTACGGGT CCATCAGCGG GAAAGTTTGT GCCATGATAG ATGGAATTGT
  421 GGATGAAGCA ATTTCCTTCG AGACGAATGC TCCAGCTGGC TGTAGCAACT CCTCCATCCT
  481 CAAATAGAAA TGACCTTTCA TATGACATTC CATCTGGGAA TGCTTGCTTG AAATAGTCAG
  541 GCATGTCGGC AGGGTACTTT GTGAAGCATC TGTTTCCATA TTGAAAGACT GTTGACAGTA
  601 TGTCAAAGGA GAATGGAAGG GGTTTTCCCT TAGGCTTGAT GATCACTAAT TCGGACGTCT
  661 GTTTCCCTTC GTAAGGNTTT CCAGTTCCTA CACCTTCGAT CGTGAACTCA TGGCCATTGA
  721 CACTGCCTTC CATACGGTAT TTCGTCCGCA TTTCTTGTGC GATGCCTTGC TTTGAATAAG
  781 ACACCATGGT GGGGNTCCCC GGGTACCGAG CTCGAATTCG TAATCATGGT CATANCTGTT
  841 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCNNCACAAC ATACGANCCG GANNCATAAA
  901 GTGTAAAGCC NGGGGNGNCN AANGANTGAN CTAANTCACA TTAATTGNGT TGCGCNCACN
961 GNCNNNNTTN CANNNNGGAA ANNNNNCNNG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2021.10.18

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