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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1)

CoralHue™ humanized dimeric Keima-Red (phdKeima-Red-S1)

Catalog number RDB18286
Resource name CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1)
Alternative name AM-V0104M
Clone info. ・For subcloning.
・The codons are humanized.
・Dimeric Keima-Red exhibits an extremely large Stokes shift(176 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/616
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18286zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18286_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18286 CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18286_B0Krp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18286_B0Kra.seq check
            >D07039F1_B0Kr_7_lacIpro-B_F_A10_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNT CNGTGAGCGA GGAAGCGGAA GAGCGCCCAA
               61 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
              121 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
              181 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
              241 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGAATTCGAG CTCGGTACCC
              301 GGGGATCCCC ACCATGGTGA GCGTGATCGC CAAGCAGATG ACCTACAAGG TGTACATGTC
              361 CGGCACCGTG AACGGCCACT ACTTCGAGGT GGAGGGCGAC GGCAAGGGCA AGCCCTACGA
              421 GGGCGAGCAG ACCGTGAAGC TGACCGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA
              481 CATCCTGTCC CCCCTGTTCC AGTACGGCAG CATCCCCTTC ACCAAGTACC CCGAGGACAT
              541 CCCCGACTAC GTGAAGCAGA GCTTCCCCGA GGGCTACACC TGGGAGAGGA CCATGAACTT
              601 CGAGGACGGC GCCGTGTGCA CCGTGAGCAA CGACTCCAGC ATCCAGGGCA ACTGCTTCAT
              661 CTACAACGTG AAGATCAGCG GCACCAACTT CCCCCCCAAC GGCCCCGTGA TGCAGAAGAA
              721 GACCCAGGGC TGGGAGCCCA GCACCGAGAG GCTGTTCGCC AGGGACGGAA TGCTGATCGG
              781 CAACGACTAC NTGGCCCTGA AGCTGGANGG CGGCGGNCAC TACCTGTGCG AGTTCAAGTC
              841 CACCTACNAG GNCNANAAGC CCGTGAGGAN GNCCGGNTAC CACTACATCG ACAGGAAGCT
              901 GGACNTGANC ANNCACNANA GGGNNTACAC CTNCGNGNAN CANTGCGAGA TCGCCATCNC
              961 CNGGCACTCC CTGNTGGGNT ANNTGCNNNC ATGCNANCNT GGCNNNNGNN NGNCGTTTTN
            1021 NNNCGNCNNG ACNGGGAAAN CCNNNNGNNN NNNNNNN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18286_B0Krb.seq check
            >D07039F1_B0Kr_7_pUC-M13_-100_H05_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTTCCCNGNC NCGACGTTGT
               61 AAAANNNNNN CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGG AGTGCCTGGC
              121 GATGGCGATC TCGCACTGCT CCACGGAGGT GTAGTCCCTG TTGTGGCTGG TCACGTCCAG
              181 CTTCCTGTCG ATGTAGTGGT AGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGGA
              241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
              301 GATCAGCATT CCGTCCCTGG CGAACAGCCT CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
              361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTGGTGCCG CTGATCTTCA CGTTGTAGAT
              421 GAAGCAGTTG CCCTGGATGC TGGAGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
              481 GTTCATGGTC CTCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTCACGT AGTCGGGGAT
              541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AACAGGGGGG ACAGGATGTC
              601 CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC
              661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGGA
              721 CATGTACACC TTGTAGGTCA TCTGCTTGNN NATCACGCTC ACCATGGTGG GGATCCCCGG
              781 GTACCGAGCT CGAATTCGTA ATCATGGTCA TAGCTGTTTN CNTGNNNNNN NNTGNTATCC
              841 GCTCACAATT CNNCACNANN TACNANCCNG AAGCATAAAG TGTAAAGCCT GGGGNGNCTN
              901 NTGANNGANC NNNTNNNNTT NATTGNGTTN NNGCTCNCTG NCCNNNTTNN CNGNCNGGAN
              961 NCNNGNCGNG NCANCTGNNN TAATGANNCN GNCNACGCNN NGGGGANNNN GNGGTTNGNG
            1021 NATNGNNNNN NNNNTNCNNT TNCTCGCNNN NNNGANNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl