CoralHue™ humanized dimeric Keima-Red (phdKeima-Red-S1)
Alternative name | AM-V0104M |
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Clone info. | ・For subcloning. ・The codons are humanized. ・Dimeric Keima-Red exhibits an extremely large Stokes shift(176 nm) and is stable under usual aerobic conditions. ・Ex/Em(nm): 440/616 |
Vector backbone | S1 (plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Ampicillin |
Gene/insert name | Montipora. sp red fluorescent protein cDNA |
Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Sequence (full) | RDB18286zzk01.seq provided by the depositor | Additional map |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18286 | CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1) | DNA solution |
Materials & Methods section:
The CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18286). |
Reference section:
Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18286_B0Krp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: lacIpro-B_F (Pr0724) Region: MCS, insert 5' Sequence file: RDB18286_B0Kra.seq |
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>D07039F1_B0Kr_7_lacIpro-B_F_A10_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNT CNGTGAGCGA GGAAGCGGAA GAGCGCCCAA 61 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT 121 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 181 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG 241 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGAATTCGAG CTCGGTACCC 301 GGGGATCCCC ACCATGGTGA GCGTGATCGC CAAGCAGATG ACCTACAAGG TGTACATGTC 361 CGGCACCGTG AACGGCCACT ACTTCGAGGT GGAGGGCGAC GGCAAGGGCA AGCCCTACGA 421 GGGCGAGCAG ACCGTGAAGC TGACCGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA 481 CATCCTGTCC CCCCTGTTCC AGTACGGCAG CATCCCCTTC ACCAAGTACC CCGAGGACAT 541 CCCCGACTAC GTGAAGCAGA GCTTCCCCGA GGGCTACACC TGGGAGAGGA CCATGAACTT 601 CGAGGACGGC GCCGTGTGCA CCGTGAGCAA CGACTCCAGC ATCCAGGGCA ACTGCTTCAT 661 CTACAACGTG AAGATCAGCG GCACCAACTT CCCCCCCAAC GGCCCCGTGA TGCAGAAGAA 721 GACCCAGGGC TGGGAGCCCA GCACCGAGAG GCTGTTCGCC AGGGACGGAA TGCTGATCGG 781 CAACGACTAC NTGGCCCTGA AGCTGGANGG CGGCGGNCAC TACCTGTGCG AGTTCAAGTC 841 CACCTACNAG GNCNANAAGC CCGTGAGGAN GNCCGGNTAC CACTACATCG ACAGGAAGCT 901 GGACNTGANC ANNCACNANA GGGNNTACAC CTNCGNGNAN CANTGCGAGA TCGCCATCNC 961 CNGGCACTCC CTGNTGGGNT ANNTGCNNNC ATGCNANCNT GGCNNNNGNN NGNCGTTTTN 1021 NNNCGNCNNG ACNGGGAAAN CCNNNNGNNN NNNNNNN // |
Primer: pUC-M13_-100 (Pr0310) Region: MCS, insert 3' Sequence file: RDB18286_B0Krb.seq |
>D07039F1_B0Kr_7_pUC-M13_-100_H05_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTTCCCNGNC NCGACGTTGT 61 AAAANNNNNN CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGG AGTGCCTGGC 121 GATGGCGATC TCGCACTGCT CCACGGAGGT GTAGTCCCTG TTGTGGCTGG TCACGTCCAG 181 CTTCCTGTCG ATGTAGTGGT AGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGGA 241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC 301 GATCAGCATT CCGTCCCTGG CGAACAGCCT CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT 361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTGGTGCCG CTGATCTTCA CGTTGTAGAT 421 GAAGCAGTTG CCCTGGATGC TGGAGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA 481 GTTCATGGTC CTCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTCACGT AGTCGGGGAT 541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AACAGGGGGG ACAGGATGTC 601 CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC 661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGGA 721 CATGTACACC TTGTAGGTCA TCTGCTTGNN NATCACGCTC ACCATGGTGG GGATCCCCGG 781 GTACCGAGCT CGAATTCGTA ATCATGGTCA TAGCTGTTTN CNTGNNNNNN NNTGNTATCC 841 GCTCACAATT CNNCACNANN TACNANCCNG AAGCATAAAG TGTAAAGCCT GGGGNGNCTN 901 NTGANNGANC NNNTNNNNTT NATTGNGTTN NNGCTCNCTG NCCNNNTTNN CNGNCNGGAN 961 NCNNGNCGNG NCANCTGNNN TAATGANNCN GNCNACGCNN NGGGGANNNN GNGGTTNGNG 1021 NATNGNNNNN NNNNTNCNNT TNCTCGCNNN NNNGANNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP] |
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