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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1)

CoralHue™ humanized dimeric Keima-Red (phdKeima-Red-S1)

Catalog number RDB18286
Resource name CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1)
Alternative name AM-V0104M
Clone info. ・For subcloning.
・The codons are humanized.
・Dimeric Keima-Red exhibits an extremely large Stokes shift(176 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/616
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18286 CoralHue(TM) humanized dimeric Keima-Red (phdKeima-Red-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18286zzk01.seq
Electronic file Map RDB18286_map.png

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18286_B0Krp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18286_B0Kra.seq check
>D07039F1_B0Kr_7_lacIpro-B_F_A10_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNT CNGTGAGCGA GGAAGCGGAA GAGCGCCCAA
   61 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
  121 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
  181 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
  241 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGAATTCGAG CTCGGTACCC
  301 GGGGATCCCC ACCATGGTGA GCGTGATCGC CAAGCAGATG ACCTACAAGG TGTACATGTC
  361 CGGCACCGTG AACGGCCACT ACTTCGAGGT GGAGGGCGAC GGCAAGGGCA AGCCCTACGA
  421 GGGCGAGCAG ACCGTGAAGC TGACCGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA
  481 CATCCTGTCC CCCCTGTTCC AGTACGGCAG CATCCCCTTC ACCAAGTACC CCGAGGACAT
  541 CCCCGACTAC GTGAAGCAGA GCTTCCCCGA GGGCTACACC TGGGAGAGGA CCATGAACTT
  601 CGAGGACGGC GCCGTGTGCA CCGTGAGCAA CGACTCCAGC ATCCAGGGCA ACTGCTTCAT
  661 CTACAACGTG AAGATCAGCG GCACCAACTT CCCCCCCAAC GGCCCCGTGA TGCAGAAGAA
  721 GACCCAGGGC TGGGAGCCCA GCACCGAGAG GCTGTTCGCC AGGGACGGAA TGCTGATCGG
  781 CAACGACTAC NTGGCCCTGA AGCTGGANGG CGGCGGNCAC TACCTGTGCG AGTTCAAGTC
  841 CACCTACNAG GNCNANAAGC CCGTGAGGAN GNCCGGNTAC CACTACATCG ACAGGAAGCT
  901 GGACNTGANC ANNCACNANA GGGNNTACAC CTNCGNGNAN CANTGCGAGA TCGCCATCNC
  961 CNGGCACTCC CTGNTGGGNT ANNTGCNNNC ATGCNANCNT GGCNNNNGNN NGNCGTTTTN
1021 NNNCGNCNNG ACNGGGAAAN CCNNNNGNNN NNNNNNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18286_B0Krb.seq check
>D07039F1_B0Kr_7_pUC-M13_-100_H05_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTTCCCNGNC NCGACGTTGT
   61 AAAANNNNNN CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGG AGTGCCTGGC
  121 GATGGCGATC TCGCACTGCT CCACGGAGGT GTAGTCCCTG TTGTGGCTGG TCACGTCCAG
  181 CTTCCTGTCG ATGTAGTGGT AGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGGA
  241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
  301 GATCAGCATT CCGTCCCTGG CGAACAGCCT CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
  361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTGGTGCCG CTGATCTTCA CGTTGTAGAT
  421 GAAGCAGTTG CCCTGGATGC TGGAGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
  481 GTTCATGGTC CTCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTCACGT AGTCGGGGAT
  541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AACAGGGGGG ACAGGATGTC
  601 CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC
  661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGGA
  721 CATGTACACC TTGTAGGTCA TCTGCTTGNN NATCACGCTC ACCATGGTGG GGATCCCCGG
  781 GTACCGAGCT CGAATTCGTA ATCATGGTCA TAGCTGTTTN CNTGNNNNNN NNTGNTATCC
  841 GCTCACAATT CNNCACNANN TACNANCCNG AAGCATAAAG TGTAAAGCCT GGGGNGNCTN
  901 NTGANNGANC NNNTNNNNTT NATTGNGTTN NNGCTCNCTG NCCNNNTTNN CNGNCNGGAN
  961 NCNNGNCGNG NCANCTGNNN TAATGANNCN GNCNACGCNN NGGGGANNNN GNGGTTNGNG
1021 NATNGNNNNN NNNNTNCNNT TNCTCGCNNN NNNGANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2020.12.28

GNP_filter3_RDBDEP_html_201222.pl