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CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1)

CoralHue™ dimeric Keima-Red (pdKeima-Red-S1)

Catalog number RDB18285
Resource name CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1)
Alternative name AM-V0101M
Clone info. ・For subcloning.
・Dimeric Keima-Red exhibits an extremely large Stokes shift(176 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/616
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18285 CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18285zzk01.seq
Electronic file Map RDB18285_map.png

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18285_B0Krp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18285_B0Kra.seq check
>D07039E9_B0Kr_4_lacIpro-B_F_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNANCGNN TCNGTGAGCG AGGNANCGGA AGAGCGCCCA
   61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
  121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
  181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
  241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGAATTCGA GCTCGGTACC
  301 CGGGGATCCC CACCATGGTG AGTGTGATCG CTAAACAAAT GACCTACAAG GTTTATATGT
  361 CAGGCACGGT CAATGGACAC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA AAGCCTTACG
  421 AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA TTTGCTTGGG
  481 ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC CCTGAAGACA
  541 TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG ACCATGAACT
  601 TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC AACTGTTTCA
  661 TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT ATGCAGAAGA
  721 AGACACAGGG CTGGGAACCC AGCACTGANC GTCTCTTTGC ACGAGATGGA ATGCTGATAG
  781 GAAACGATTA TATGGCTCTG AAGTTGGAAG GANGTGGTCN NTATTTGTGT GAATTTNAAT
  841 CTACTTACNA GGNAAAGAAN CCTGTGAGGA TGCCAGGGTA TCACTATATT GANCGCAACT
  901 GGATGTNNNN NGTCNNNNCN GGGNNTACNC ATCTGTTGAN CAGTGTGAAA TAGCCNNNGN
  961 NCGNCACTCN TTGNTCNGNT AACTGNNNNA TGCANNCTTG GCACTGGNCN GNCGTTTTAN
 1021 ANCNTCNNGN NNNGNNAANN CCNNNNNNNN CCNNACNNAN NNGNNTNGNN NCNNANNNNN
1081 NTNNNNNNGC NGGNNNANNN NNNNNNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18285_B0Krb.seq check
>D07039E9_B0Kr_4_pUC-M13_-100_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGNANGC NNGGGTTTTC CNNGTCNCGA CGTTGTAAAA
   61 CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGTTAACCGA GCAAAGAGTG GCGTGCAATG
  121 GCTATTTCAC ACTGCTCAAC AGATGTGTAA TCCCTGTTGT GACTGGTTAC ATCCAGTTTG
  181 CGGTCAATAT AGTGATACCC TGGCATCCTC ACAGGCTTCT TTGCCTTGTA AGTAGATTTA
  241 AATTCACACA AATAGTGACC ACCTCCTTCC AACTTCAGAG CCATATAATC GTTTCCTATC
  301 AGCATTCCAT CTCGTGCAAA GAGACGCTCA GTGCTGGGTT CCCAGCCCTG TGTCTTCTTC
  361 TGCATAACAG GTCCATTGGG AGGAAAGTTC GTACCAGAGA TTTTGACATT GTAGATGAAA
  421 CAGTTGCCTT GGATGCTGGA ATCATTGCTG ACAGTACACA CTGCACCATC TTCAAAGTTC
  481 ATGGTCCTCT CCCATGTATA TCCCTCAGGG AATGACTGCT TTACATAATC AGGGATGTCT
  541 TCAGGGTACT TGGTGAATGG TATGCTTCCG TACTGAAACA GTGGTGATAA TATATCCCAA
  601 GCAAATGGCA GAGGTCCACC CTTGGTGACA GTGAGCTTTA CTGTCTGCTC TCCCTCGTAA
  661 GGCTTTCCTT TTCCATCGCC TTCGACCTCA AAGTAGTGTC CATTGACCGT GCCTGACATA
  721 TAAACCTTGT AGGTCATTTG TTTAGCGATC ACACTCACCA TGGTGGGGAT CCCCGGGTAN
  781 CGAGCTCGAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGN TATCCGCTCA
  841 CAATTCCNCA CAACATACNA GCCGGAAGCA TAAAGTGNAA AGCCTGGGGN GNCNNATGAN
  901 TGANCTAACT CACANNNNTT GCGTTGCGCT CACTGCCCGC TTTNCAGNNN GNAAANCNGN
  961 CNNGNCNNNT GCNNTNATGA ANCNGNCNAN CGCGCGGGNN NANGNGGTTN NNGNATNGGG
 1021 NNNTCTTNNN NTNNNNCNNN NNNNNGNNNN NCTGCNNNCN GNNCGNNCNG NNGNGGNNAN
1081 NGNNATCANN TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.01.15

GNP_filter3_RDBDEP_html_201222.pl