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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1)

CoralHue™ dimeric Keima-Red (pdKeima-Red-S1)

Catalog number RDB18285
Resource name CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1)
Alternative name AM-V0101M
Clone info. ・For subcloning.
・Dimeric Keima-Red exhibits an extremely large Stokes shift(176 nm) and is stable under usual aerobic conditions.
・Ex/Em(nm): 440/616
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18285zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18285_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18285 CoralHue(TM) dimeric Keima-Red (pdKeima-Red-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18285_B0Krp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18285_B0Kra.seq check
            >D07039E9_B0Kr_4_lacIpro-B_F_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNANCGNN TCNGTGAGCG AGGNANCGGA AGAGCGCCCA
               61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
              121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
              181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
              241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGAATTCGA GCTCGGTACC
              301 CGGGGATCCC CACCATGGTG AGTGTGATCG CTAAACAAAT GACCTACAAG GTTTATATGT
              361 CAGGCACGGT CAATGGACAC TACTTTGAGG TCGAAGGCGA TGGAAAAGGA AAGCCTTACG
              421 AGGGAGAGCA GACAGTAAAG CTCACTGTCA CCAAGGGTGG ACCTCTGCCA TTTGCTTGGG
              481 ATATATTATC ACCACTGTTT CAGTACGGAA GCATACCATT CACCAAGTAC CCTGAAGACA
              541 TCCCTGATTA TGTAAAGCAG TCATTCCCTG AGGGATATAC ATGGGAGAGG ACCATGAACT
              601 TTGAAGATGG TGCAGTGTGT ACTGTCAGCA ATGATTCCAG CATCCAAGGC AACTGTTTCA
              661 TCTACAATGT CAAAATCTCT GGTACGAACT TTCCTCCCAA TGGACCTGTT ATGCAGAAGA
              721 AGACACAGGG CTGGGAACCC AGCACTGANC GTCTCTTTGC ACGAGATGGA ATGCTGATAG
              781 GAAACGATTA TATGGCTCTG AAGTTGGAAG GANGTGGTCN NTATTTGTGT GAATTTNAAT
              841 CTACTTACNA GGNAAAGAAN CCTGTGAGGA TGCCAGGGTA TCACTATATT GANCGCAACT
              901 GGATGTNNNN NGTCNNNNCN GGGNNTACNC ATCTGTTGAN CAGTGTGAAA TAGCCNNNGN
              961 NCGNCACTCN TTGNTCNGNT AACTGNNNNA TGCANNCTTG GCACTGGNCN GNCGTTTTAN
             1021 ANCNTCNNGN NNNGNNAANN CCNNNNNNNN CCNNACNNAN NNGNNTNGNN NCNNANNNNN
            1081 NTNNNNNNGC NGGNNNANNN NNNNNNN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18285_B0Krb.seq check
            >D07039E9_B0Kr_4_pUC-M13_-100_D05_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGGGNANGC NNGGGTTTTC CNNGTCNCGA CGTTGTAAAA
               61 CGACGGCCAG TGCCAAGCTT GCATGCCTGC AGTTAACCGA GCAAAGAGTG GCGTGCAATG
              121 GCTATTTCAC ACTGCTCAAC AGATGTGTAA TCCCTGTTGT GACTGGTTAC ATCCAGTTTG
              181 CGGTCAATAT AGTGATACCC TGGCATCCTC ACAGGCTTCT TTGCCTTGTA AGTAGATTTA
              241 AATTCACACA AATAGTGACC ACCTCCTTCC AACTTCAGAG CCATATAATC GTTTCCTATC
              301 AGCATTCCAT CTCGTGCAAA GAGACGCTCA GTGCTGGGTT CCCAGCCCTG TGTCTTCTTC
              361 TGCATAACAG GTCCATTGGG AGGAAAGTTC GTACCAGAGA TTTTGACATT GTAGATGAAA
              421 CAGTTGCCTT GGATGCTGGA ATCATTGCTG ACAGTACACA CTGCACCATC TTCAAAGTTC
              481 ATGGTCCTCT CCCATGTATA TCCCTCAGGG AATGACTGCT TTACATAATC AGGGATGTCT
              541 TCAGGGTACT TGGTGAATGG TATGCTTCCG TACTGAAACA GTGGTGATAA TATATCCCAA
              601 GCAAATGGCA GAGGTCCACC CTTGGTGACA GTGAGCTTTA CTGTCTGCTC TCCCTCGTAA
              661 GGCTTTCCTT TTCCATCGCC TTCGACCTCA AAGTAGTGTC CATTGACCGT GCCTGACATA
              721 TAAACCTTGT AGGTCATTTG TTTAGCGATC ACACTCACCA TGGTGGGGAT CCCCGGGTAN
              781 CGAGCTCGAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGN TATCCGCTCA
              841 CAATTCCNCA CAACATACNA GCCGGAAGCA TAAAGTGNAA AGCCTGGGGN GNCNNATGAN
              901 TGANCTAACT CACANNNNTT GCGTTGCGCT CACTGCCCGC TTTNCAGNNN GNAAANCNGN
              961 CNNGNCNNNT GCNNTNATGA ANCNGNCNAN CGCGCGGGNN NANGNGGTTN NNGNATNGGG
             1021 NNNTCTTNNN NTNNNNCNNN NNNNNGNNNN NCTGCNNNCN GNNCGNNCNG NNGNGGNNAN
            1081 NGNNATCANN TNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl