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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL)

CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-MCL)

Catalog number RDB18283
Resource name CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL)
Alternative name AM-V0099M
Clone info. ・Fluorescent protein-Linker-MCS/For mammalian expression of the fluorescent protein or its fusion.
・The codons are humanized.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone MCL (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18283zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18283_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18283 CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18283_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, insert 5'
            Sequence file: RDB18283_B0H4a.seq check
            >D07039E7_B0H4_2_CMV_Forward_D01_10_ABI24.ab1
                1 NNNNNNNNNN NNNNTNNNNG CNNNGGGCGG TANGCGTGTA CGGNGGGNNG TCTATATAAG
               61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCATTGC CACCATGGTG AGCGTGATCG
              121 CCAAGCAGAT GACCTACAAG GTGTACATGA GCGGCACCGT GAACGGCCAC TACTTCGAGG
              181 TGGAGGGCGA CGGCAAGGGC AAGCCCTACG AGGGCGAGCA GACCGTGAAG CTGACCGTGA
              241 CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGAG CCCCCAGCTC CAGTACGGCA
              301 GCATCCCCTT CACCAAGTAC CCCGAGGACA TCCCCGACTA CTTCAAGCAG AGCTTCCCCG
              361 AGGGCTACAC CTGGGAGCGC AGCATGAACT TCGAGGACGG CGCCGTGTGC ACCGTGAGCA
              421 ACGACAGCAG CATCCAGGGC AACTGCTTCA TCTACAACGT GAAGATCAGC GGCGAGAACT
              481 TCCCCCCCAA CGGCCCCGTG ATGCAGAAGA AGACCCAGGG CTGGGAGCCC AGCACCGAGC
              541 GCCTGTTCGC CCGCGACGGA ATGCTGATCG GCAACGACTA CATGGCCCTG AAGCTGGAGG
              601 GCGGCGGCCA CTACCTGTGC GAGTTCAAGA GCACCTACAA GGCCAAGAAG CCCGTGAGGN
              661 TGCCCGGCCG CCACGAGATC GACCGCAAGC TGGACGTGNC CAGCCACAAC CGCGACTACA
              721 CCAGCGTGGA NCAGTGCGAG ATCGCCATCG CCCGCCACAG CCTGCTGGGC ACCGGTAATT
              781 CCGCTGACGG CGGCGGANGN NCGGGNGGTA GTGGTGGTTC ANGANGANGN TCGNNCCAAG
              841 GNNGNNNCTC ANGNNNCGGA ACNNCAGCAN ANAATTCNGG GAAANTCGAN NNNNAANCNN
            901 GNANNAANCG GCCNNNNNNC TNNNTCNTNA TCAGNNNNNN NNNN
            //
            Primer: SV40polyA_R2
            Region: MCS, Linker, insert 3'
            Sequence file: RDB18283_B0H4b.seq check
            >D07039E7_B0H4_2_SV40polyA-R2_E01_13_ABI24.ab1
                1 NNNNNNNNNC NNCANANNNN GTTNNNANCN ACNATTGCAT TCATTTTATG TTTCAGGTTC
               61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
              121 ATTATGATCT AGAGTCGCGG CCGCTTATTC AAGCTTTGTT CTCGAGTTTC CCGAATTCTC
              181 TGCTGCAGTT CCGGTACCTG AGGATCCTCC TTGGGTCGAT CCTCCTCCTG AACCACCACT
              241 ACCACCCGAT CCTCCGCCGC CGTCAGCGGA ATTACCGGTG CCCAGCAGGC TGTGGCGGGC
              301 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG
              361 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT
              421 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
              481 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
              541 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT
              601 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
              661 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAANCTC TGCTTGAAGT AGTCGGGGNT
              721 GTCCTCGGGG TACTTGGTGA ANGGGANGCT GCCGTACTGG ANCTGGGGGC TCANGATGTC
              781 CNANGCGAAN GGCANGGGGC CACCNNNGGN CACGGNCAGC TTCACGGTCT GCTCGCCCTC
              841 NNANGGCTNG CCNTTGCCGT CNCCNNNCAC CNCNNANTAN TGGCCNNTCA CGGNNCCNCN
            901 CANNNNCACC NNNNNNNNNT NNNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori, NeoR_KanR
            Sequence file: RDB18283_B0H4c.seq check
            >D07039E7_B0H4_2_SV40pro_ori_F_F01_16_ABI24.ab1
                1 NNNNNNNNNN NGNNGCCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
               61 GAGGCCTAGG CTTTTGCAAA GATCATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG
              121 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG
              181 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG
              241 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG
              301 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG
              361 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC
              421 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC
              481 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC
              541 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC
              601 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG
              661 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT
              721 TTTCTGGANT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN GACATANCGT
              781 TGGCTACCCG NNNTATTGCT GAANANCTTG GCGGCGAATG GGNCTGANCG CTTCCTCGNG
              841 CTTTACNGNA TCGCCGCTCC NNANNCGCAN CGCATCGCCT NNNANNNNCN TNNNNACNAN
            901 TNNTTNNNNN NGGNNNNNNG GGGNNNNNNA NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl