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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL) (#RDB18283)

CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-MCL)

Alternative name AM-V0099M
Clone info. ・Fluorescent protein-Linker-MCS/For mammalian expression of the fluorescent protein or its fusion.
・The codons are humanized.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone MCL (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18283zzk02.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18283 CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-MCL) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18283).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18283_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB18283_B0H4a.seq check
>D07039E7_B0H4_2_CMV_Forward_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNTNNNNG CNNNGGGCGG TANGCGTGTA CGGNGGGNNG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCATTGC CACCATGGTG AGCGTGATCG
  121 CCAAGCAGAT GACCTACAAG GTGTACATGA GCGGCACCGT GAACGGCCAC TACTTCGAGG
  181 TGGAGGGCGA CGGCAAGGGC AAGCCCTACG AGGGCGAGCA GACCGTGAAG CTGACCGTGA
  241 CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGAG CCCCCAGCTC CAGTACGGCA
  301 GCATCCCCTT CACCAAGTAC CCCGAGGACA TCCCCGACTA CTTCAAGCAG AGCTTCCCCG
  361 AGGGCTACAC CTGGGAGCGC AGCATGAACT TCGAGGACGG CGCCGTGTGC ACCGTGAGCA
  421 ACGACAGCAG CATCCAGGGC AACTGCTTCA TCTACAACGT GAAGATCAGC GGCGAGAACT
  481 TCCCCCCCAA CGGCCCCGTG ATGCAGAAGA AGACCCAGGG CTGGGAGCCC AGCACCGAGC
  541 GCCTGTTCGC CCGCGACGGA ATGCTGATCG GCAACGACTA CATGGCCCTG AAGCTGGAGG
  601 GCGGCGGCCA CTACCTGTGC GAGTTCAAGA GCACCTACAA GGCCAAGAAG CCCGTGAGGN
  661 TGCCCGGCCG CCACGAGATC GACCGCAAGC TGGACGTGNC CAGCCACAAC CGCGACTACA
  721 CCAGCGTGGA NCAGTGCGAG ATCGCCATCG CCCGCCACAG CCTGCTGGGC ACCGGTAATT
  781 CCGCTGACGG CGGCGGANGN NCGGGNGGTA GTGGTGGTTC ANGANGANGN TCGNNCCAAG
  841 GNNGNNNCTC ANGNNNCGGA ACNNCAGCAN ANAATTCNGG GAAANTCGAN NNNNAANCNN
901 GNANNAANCG GCCNNNNNNC TNNNTCNTNA TCAGNNNNNN NNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: MCS, Linker, insert 3'
Sequence file: RDB18283_B0H4b.seq check
>D07039E7_B0H4_2_SV40polyA-R2_E01_13_ABI24.ab1
    1 NNNNNNNNNC NNCANANNNN GTTNNNANCN ACNATTGCAT TCATTTTATG TTTCAGGTTC
   61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG
  121 ATTATGATCT AGAGTCGCGG CCGCTTATTC AAGCTTTGTT CTCGAGTTTC CCGAATTCTC
  181 TGCTGCAGTT CCGGTACCTG AGGATCCTCC TTGGGTCGAT CCTCCTCCTG AACCACCACT
  241 ACCACCCGAT CCTCCGCCGC CGTCAGCGGA ATTACCGGTG CCCAGCAGGC TGTGGCGGGC
  301 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG
  361 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT
  421 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
  481 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
  541 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT
  601 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
  661 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAANCTC TGCTTGAAGT AGTCGGGGNT
  721 GTCCTCGGGG TACTTGGTGA ANGGGANGCT GCCGTACTGG ANCTGGGGGC TCANGATGTC
  781 CNANGCGAAN GGCANGGGGC CACCNNNGGN CACGGNCAGC TTCACGGTCT GCTCGCCCTC
  841 NNANGGCTNG CCNTTGCCGT CNCCNNNCAC CNCNNANTAN TGGCCNNTCA CGGNNCCNCN
901 CANNNNCACC NNNNNNNNNT NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18283_B0H4c.seq check
>D07039E7_B0H4_2_SV40pro_ori_F_F01_16_ABI24.ab1
    1 NNNNNNNNNN NGNNGCCTCN GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTAGG CTTTTGCAAA GATCATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG
  121 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG
  181 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG
  241 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG
  301 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG
  361 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC
  421 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC
  481 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC
  541 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC
  601 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG
  661 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT
  721 TTTCTGGANT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAN GACATANCGT
  781 TGGCTACCCG NNNTATTGCT GAANANCTTG GCGGCGAATG GGNCTGANCG CTTCCTCGNG
  841 CTTTACNGNA TCGCCGCTCC NNANNCGCAN CGCATCGCCT NNNANNNNCN TNNNNACNAN
901 TNNTTNNNNN NGGNNNNNNG GGGNNNNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]