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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) (#RDB18282)

CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-S1)

Alternative name AM-V0094M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18282zzk02.seq provided by the depositor
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18282 CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18282).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18282_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18282_B0H4a.seq check
>D07039E6_B0H4_18_lacIpro-B_F_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNCGNNNCG NNNCNGTGNG CGNGGAAGCG GAAGAGCGCC CAATACGCAA
   61 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
  121 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
  181 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
  241 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGAATTC GAGCTCGGTA CCCGGGGATC
  301 CCCACCATGG TGAGCGTGAT CGCCAAGCAG ATGACCTACA AGGTGTACAT GAGCGGCACC
  361 GTGAACGGCC ACTACTTCGA GGTGGAGGGC GACGGCAAGG GCAAGCCCTA CGAGGGCGAG
  421 CAGACCGTGA AGCTGACCGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG
  481 AGCCCCCAGC TCCAGTACGG CAGCATCCCC TTCACCAAGT ACCCCGAGGA CATCCCCGAC
  541 TACTTCAAGC AGAGCTTCCC CGAGGGCTAC ACCTGGGAGC GCAGCATGAA CTTCGAGGAC
  601 GGCGCCGTGT GCACCGTGAG CAACGACAGC AGCATCCAGG GCAACTGCTT CATCTACAAC
  661 GTGAAGATCA GCGGCGAGAA CTTCCCCCCC AACGGCCCCG TGATGCAGAA GAAGANCCAG
  721 GGCTGGGAGC CCAGCACCGA GCGCCTGTTC GCCCGCGACG GAATGCTGAT CGGCAACGAC
  781 TACATGGCCC TGAAGCTGGA GGGCGGCGGC CACTACCTGT GCGANTTCAA GANCACCTAC
  841 AANGCCNAGA ANCCCGNGAN GATGCCCGGN CGCCACGANA TCGANCGCAN NCTGGANGNG
  901 NNCAGCCNCA ACCGCNACTA CNCNAGCGNN GANCANNGNG AGATCGCCAT CGNCCNNNNC
961 ANCCNGNNGG NNNNNNNGNN NNNNNGNNNG NNNNNNNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 3'
Sequence file: RDB18282_B0H4b.seq check
>D07039_E6_B0H4_18_pUC-M13_-100_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNNNNNNC NNNANGTTGT
   61 AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGC TGTGGCGGGC
  121 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG
  181 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT
  241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
  301 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
  361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT
  421 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
  481 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTGAAGT AGTCGGGGAT
  541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AGCTGGGGGC TCAGGATGTC
  601 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC
  661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGCT
  721 CATGTACACC TTGTANGTCA TCTGCTTGGC GATCACGCTC ACCATGGTGG GGATCCCCGG
  781 GTNCCGNNNN NNAATTCGTA ATCNNGGTCA TAGCTGTTTC CTGNNNNNAA TTGTTATCCG
  841 CTCNCNNTTN CACACNAACA TACGANCCNG AAGCATANAG TGTNAANGCC NGGGGNGCCT
  901 AATGAGTGAN CTNANNTCAC ATTNANTTGC GTNGCNCTCA CTGNCCGCNT TNCCAGNCNG
  961 GAAACCNGTC NNGNCAGCTG CNTNAATNNA NTNNNCNAAC GCGCGGGGNN ANGNNGNNTN
1021 GNGNNNTNGG GCNCTNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]