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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1)

CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-S1)

Catalog number RDB18282
Resource name CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1)
Alternative name AM-V0094M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank
External Database
Montipora. sp red fluorescent protein
            Reference sequence
              
             
            Sequence (full) RDB18282zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18282_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18282 CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18282_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18282_B0H4a.seq check
            >D07039E6_B0H4_18_lacIpro-B_F_G06_21_ABI24.ab1
                1 NNNNNNNNNN NNNCGNNNCG NNNCNGTGNG CGNGGAAGCG GAAGAGCGCC CAATACGCAA
               61 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
              121 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
              181 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
              241 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGAATTC GAGCTCGGTA CCCGGGGATC
              301 CCCACCATGG TGAGCGTGAT CGCCAAGCAG ATGACCTACA AGGTGTACAT GAGCGGCACC
              361 GTGAACGGCC ACTACTTCGA GGTGGAGGGC GACGGCAAGG GCAAGCCCTA CGAGGGCGAG
              421 CAGACCGTGA AGCTGACCGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG
              481 AGCCCCCAGC TCCAGTACGG CAGCATCCCC TTCACCAAGT ACCCCGAGGA CATCCCCGAC
              541 TACTTCAAGC AGAGCTTCCC CGAGGGCTAC ACCTGGGAGC GCAGCATGAA CTTCGAGGAC
              601 GGCGCCGTGT GCACCGTGAG CAACGACAGC AGCATCCAGG GCAACTGCTT CATCTACAAC
              661 GTGAAGATCA GCGGCGAGAA CTTCCCCCCC AACGGCCCCG TGATGCAGAA GAAGANCCAG
              721 GGCTGGGAGC CCAGCACCGA GCGCCTGTTC GCCCGCGACG GAATGCTGAT CGGCAACGAC
              781 TACATGGCCC TGAAGCTGGA GGGCGGCGGC CACTACCTGT GCGANTTCAA GANCACCTAC
              841 AANGCCNAGA ANCCCGNGAN GATGCCCGGN CGCCACGANA TCGANCGCAN NCTGGANGNG
              901 NNCAGCCNCA ACCGCNACTA CNCNAGCGNN GANCANNGNG AGATCGCCAT CGNCCNNNNC
            961 ANCCNGNNGG NNNNNNNGNN NNNNNGNNNG NNNNNNNNNN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18282_B0H4b.seq check
            >D07039_E6_B0H4_18_pUC-M13_-100_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNNNNNNC NNNANGTTGT
               61 AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGC TGTGGCGGGC
              121 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG
              181 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT
              241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
              301 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
              361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT
              421 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
              481 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTGAAGT AGTCGGGGAT
              541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AGCTGGGGGC TCAGGATGTC
              601 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC
              661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGCT
              721 CATGTACACC TTGTANGTCA TCTGCTTGGC GATCACGCTC ACCATGGTGG GGATCCCCGG
              781 GTNCCGNNNN NNAATTCGTA ATCNNGGTCA TAGCTGTTTC CTGNNNNNAA TTGTTATCCG
              841 CTCNCNNTTN CACACNAACA TACGANCCNG AAGCATANAG TGTNAANGCC NGGGGNGCCT
              901 AATGAGTGAN CTNANNTCAC ATTNANTTGC GTNGCNCTCA CTGNCCGCNT TNCCAGNCNG
              961 GAAACCNGTC NNGNCAGCTG CNTNAATNNA NTNNNCNAAC GCGCGGGGNN ANGNNGNNTN
            1021 GNGNNNTNGG GCNCTNTNNN NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl