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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1)

CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-S1)

Catalog number RDB18282
Resource name CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1)
Alternative name AM-V0094M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18282 CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB18282zzk02.seq provided by the depositor
Remarks, protocol and/or map (png) RDB18282_map.png

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18282_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18282_B0H4a.seq check
>D07039E6_B0H4_18_lacIpro-B_F_G06_21_ABI24.ab1
    1 NNNNNNNNNN NNNCGNNNCG NNNCNGTGNG CGNGGAAGCG GAAGAGCGCC CAATACGCAA
   61 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA
  121 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
  181 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
  241 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGAATTC GAGCTCGGTA CCCGGGGATC
  301 CCCACCATGG TGAGCGTGAT CGCCAAGCAG ATGACCTACA AGGTGTACAT GAGCGGCACC
  361 GTGAACGGCC ACTACTTCGA GGTGGAGGGC GACGGCAAGG GCAAGCCCTA CGAGGGCGAG
  421 CAGACCGTGA AGCTGACCGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG
  481 AGCCCCCAGC TCCAGTACGG CAGCATCCCC TTCACCAAGT ACCCCGAGGA CATCCCCGAC
  541 TACTTCAAGC AGAGCTTCCC CGAGGGCTAC ACCTGGGAGC GCAGCATGAA CTTCGAGGAC
  601 GGCGCCGTGT GCACCGTGAG CAACGACAGC AGCATCCAGG GCAACTGCTT CATCTACAAC
  661 GTGAAGATCA GCGGCGAGAA CTTCCCCCCC AACGGCCCCG TGATGCAGAA GAAGANCCAG
  721 GGCTGGGAGC CCAGCACCGA GCGCCTGTTC GCCCGCGACG GAATGCTGAT CGGCAACGAC
  781 TACATGGCCC TGAAGCTGGA GGGCGGCGGC CACTACCTGT GCGANTTCAA GANCACCTAC
  841 AANGCCNAGA ANCCCGNGAN GATGCCCGGN CGCCACGANA TCGANCGCAN NCTGGANGNG
  901 NNCAGCCNCA ACCGCNACTA CNCNAGCGNN GANCANNGNG AGATCGCCAT CGNCCNNNNC
961 ANCCNGNNGG NNNNNNNGNN NNNNNGNNNG NNNNNNNNNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18282_B0H4b.seq check
>D07039_E6_B0H4_18_pUC-M13_-100_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNNNNNNC NNNANGTTGT
   61 AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGC TGTGGCGGGC
  121 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG
  181 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT
  241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC
  301 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT
  361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT
  421 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA
  481 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTGAAGT AGTCGGGGAT
  541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AGCTGGGGGC TCAGGATGTC
  601 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC
  661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGCT
  721 CATGTACACC TTGTANGTCA TCTGCTTGGC GATCACGCTC ACCATGGTGG GGATCCCCGG
  781 GTNCCGNNNN NNAATTCGTA ATCNNGGTCA TAGCTGTTTC CTGNNNNNAA TTGTTATCCG
  841 CTCNCNNTTN CACACNAACA TACGANCCNG AAGCATANAG TGTNAANGCC NGGGGNGCCT
  901 AATGAGTGAN CTNANNTCAC ATTNANTTGC GTNGCNCTCA CTGNCCGCNT TNCCAGNCNG
  961 GAAACCNGTC NNGNCAGCTG CNTNAATNNA NTNNNCNAAC GCGCGGGGNN ANGNNGNNTN
1021 GNGNNNTNGG GCNCTNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.10.18

GNP_filter3_RDBDEP_html_210618.pl