CoralHue™ humanized monomeric Keima-Red (phmKeima-Red-S1)
Alternative name | AM-V0094M |
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Clone info. | ・For subcloning. ・The codons are humanized. ・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm). ・Ex/Em(nm): 440/620 |
Vector backbone | S1 (plasmid) |
Size of vector backbone | 2.7 kb |
Selectable markers | Ampicillin |
Gene/insert name | Montipora. sp red fluorescent protein cDNA |
Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Sequence (full) | RDB18282zzk02.seq provided by the depositor | Additional map |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18282 | CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) | DNA solution |
Materials & Methods section:
The CoralHue(TM) humanized monomeric Keima-Red (phmKeima-Red-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18282). |
Reference section:
Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18282_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: lacIpro-B_F (Pr0724) Region: MCS, insert 5' Sequence file: RDB18282_B0H4a.seq |
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>D07039E6_B0H4_18_lacIpro-B_F_G06_21_ABI24.ab1 1 NNNNNNNNNN NNNCGNNNCG NNNCNGTGNG CGNGGAAGCG GAAGAGCGCC CAATACGCAA 61 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 121 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 181 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 241 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGAATTC GAGCTCGGTA CCCGGGGATC 301 CCCACCATGG TGAGCGTGAT CGCCAAGCAG ATGACCTACA AGGTGTACAT GAGCGGCACC 361 GTGAACGGCC ACTACTTCGA GGTGGAGGGC GACGGCAAGG GCAAGCCCTA CGAGGGCGAG 421 CAGACCGTGA AGCTGACCGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG 481 AGCCCCCAGC TCCAGTACGG CAGCATCCCC TTCACCAAGT ACCCCGAGGA CATCCCCGAC 541 TACTTCAAGC AGAGCTTCCC CGAGGGCTAC ACCTGGGAGC GCAGCATGAA CTTCGAGGAC 601 GGCGCCGTGT GCACCGTGAG CAACGACAGC AGCATCCAGG GCAACTGCTT CATCTACAAC 661 GTGAAGATCA GCGGCGAGAA CTTCCCCCCC AACGGCCCCG TGATGCAGAA GAAGANCCAG 721 GGCTGGGAGC CCAGCACCGA GCGCCTGTTC GCCCGCGACG GAATGCTGAT CGGCAACGAC 781 TACATGGCCC TGAAGCTGGA GGGCGGCGGC CACTACCTGT GCGANTTCAA GANCACCTAC 841 AANGCCNAGA ANCCCGNGAN GATGCCCGGN CGCCACGANA TCGANCGCAN NCTGGANGNG 901 NNCAGCCNCA ACCGCNACTA CNCNAGCGNN GANCANNGNG AGATCGCCAT CGNCCNNNNC 961 ANCCNGNNGG NNNNNNNGNN NNNNNGNNNG NNNNNNNNNN // |
Primer: pUC-M13_-100 (Pr0310) Region: MCS, insert 3' Sequence file: RDB18282_B0H4b.seq |
>D07039_E6_B0H4_18_pUC-M13_-100_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNNNNNNC NNNANGTTGT 61 AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGTTAG CCCAGCAGGC TGTGGCGGGC 121 GATGGCGATC TCGCACTGCT CCACGCTGGT GTAGTCGCGG TTGTGGCTGG TCACGTCCAG 181 CTTGCGGTCG ATCTCGTGGC GGCCGGGCAT CCTCACGGGC TTCTTGGCCT TGTAGGTGCT 241 CTTGAACTCG CACAGGTAGT GGCCGCCGCC CTCCAGCTTC AGGGCCATGT AGTCGTTGCC 301 GATCAGCATT CCGTCGCGGG CGAACAGGCG CTCGGTGCTG GGCTCCCAGC CCTGGGTCTT 361 CTTCTGCATC ACGGGGCCGT TGGGGGGGAA GTTCTCGCCG CTGATCTTCA CGTTGTAGAT 421 GAAGCAGTTG CCCTGGATGC TGCTGTCGTT GCTCACGGTG CACACGGCGC CGTCCTCGAA 481 GTTCATGCTG CGCTCCCAGG TGTAGCCCTC GGGGAAGCTC TGCTTGAAGT AGTCGGGGAT 541 GTCCTCGGGG TACTTGGTGA AGGGGATGCT GCCGTACTGG AGCTGGGGGC TCAGGATGTC 601 CCAGGCGAAG GGCAGGGGGC CACCCTTGGT CACGGTCAGC TTCACGGTCT GCTCGCCCTC 661 GTAGGGCTTG CCCTTGCCGT CGCCCTCCAC CTCGAAGTAG TGGCCGTTCA CGGTGCCGCT 721 CATGTACACC TTGTANGTCA TCTGCTTGGC GATCACGCTC ACCATGGTGG GGATCCCCGG 781 GTNCCGNNNN NNAATTCGTA ATCNNGGTCA TAGCTGTTTC CTGNNNNNAA TTGTTATCCG 841 CTCNCNNTTN CACACNAACA TACGANCCNG AAGCATANAG TGTNAANGCC NGGGGNGCCT 901 AATGAGTGAN CTNANNTCAC ATTNANTTGC GTNGCNCTCA CTGNCCGCNT TNCCAGNCNG 961 GAAACCNGTC NNGNCAGCTG CNTNAATNNA NTNNNCNAAC GCGCGGGGNN ANGNNGNNTN 1021 GNGNNNTNGG GCNCTNTNNN NNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP] |
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