Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1) (#RDB18281)

CoralHue™ monomeric Keima-Red (pmKeima-Red-MN1)

Alternative name AM-V0093M
Clone info. ・MCS-Fluorescent protein/For mammalian expression of the fluorescent protein or its fusion.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone MN1 (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18281zzk02.seq provided by the depositor
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18281 CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18281).

Reference section:

Kogure, T., Karasawa, S., Araki, T., Saito, K., Kinjo, M., Miyawaki, A., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18281_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, MCS, insert 5'
Sequence file: RDB18281_B0H4a.seq check
>D07039E5_B0H4_6_CMV_Forward_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCATTG GATCCTCAGG TACCGGAACT
  121 GCAGCAGAGA ATTCGGGAAA CTCGAGAACA AAGCTTGGAT CAGCGGCCGC GGGGACCATG
  181 GTGAGTGTGA TCGCTAAACA AATGACCTAC AAGGTTTATA TGTCAGGCAC GGTCAATGGA
  241 CACTACTTTG AGGTCGAAGG CGATGGAAAA GGAAAGCCTT ACGAGGGAGA GCAGACAGTA
  301 AAGCTCACTG TCACCAAGGG TGGACCTCTG CCATTTGCTT GGGATATTTT ATCACCACAG
  361 CTTCAGTACG GAAGCATACC ATTCACCAAG TACCCTGAAG ACATCCCTGA TTATTTCAAG
  421 CAGTCATTCC CTGAGGGATA TACATGGGAG AGGAGCATGA ACTTTGAAGA TGGTGCAGTG
  481 TGTACTGTCA GCAATGATTC CAGCATCCAA GGCAACTGTT TCATCTACAA TGTCAAAATC
  541 TCTGGTGAGA ACTTTCCTCC CAATGGACCT GTTATGCAGA AGAAGACACA GGGCTGGGAA
  601 CCCAGCACTG AGCGTCTCTT TGCACGAGAT GGAATGCTGA TAGGAAACGA TTATATGGCT
  661 CTGAAGTTGG AAGGAGGTGG TCACTATTTG TGTGAATTTA AATCTACTTA CAAGGCAAAG
  721 AAGCCTGTGA GGATGCCAGG GCGCCACGAG ATTGACCGCA AACTGGATGT AACCAGTCAC
  781 AACAGGGANN ACACATCTGT TGAGCAGTGT GAAATAGCCA TTGCACGCCA CTCTTTGCTC
  841 GGTTAATCTA GAGGGGGCCG CGANTCTAGA TCATAATCAG CCNTACCACA TTTGNAGANG
  901 NTTTACTNGC TTNAAAAAAC CTNCCNCACC NNCCCCNNAN NNNNAAANNT AAAATGAANG
  961 NNNTTGNTGN TGNNNACNNN NTNANNGNNN NNTATNNNNN NTNNNNANNN ANNNNNNNNN
1021 N
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB18281_B0H4b.seq check
>D07039E5_B0H4_6_SV40polyA-R2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNNNNGTT AANAACNANN ATTGCATTCN TTTTATGTTT
   61 CNGGTTCNGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  121 ATGGCTGATT ATGATCTAGA GTCGCGGCCC CCTCTAGATT AACCGAGCAA AGAGTGGCGT
  181 GCAATGGCTA TTTCACACTG CTCAACAGAT GTGTAATCCC TGTTGTGACT GGTTACATCC
  241 AGTTTGCGGT CAATCTCGTG GCGCCCTGGC ATCCTCACAG GCTTCTTTGC CTTGTAAGTA
  301 GATTTAAATT CACACAAATA GTGACCACCT CCTTCCAACT TCAGAGCCAT ATAATCGTTT
  361 CCTATCAGCA TTCCATCTCG TGCAAAGAGA CGCTCAGTGC TGGGTTCCCA GCCCTGTGTC
  421 TTCTTCTGCA TAACAGGTCC ATTGGGAGGA AAGTTCTCAC CAGAGATTTT GACATTGTAG
  481 ATGAAACAGT TGCCTTGGAT GCTGGAATCA TTGCTGACAG TACACACTGC ACCATCTTCA
  541 AAGTTCATGC TCCTCTCCCA TGTATATCCC TCAGGGAATG ACTGCTTGAA ATAATCAGGG
  601 ATGTCTTCAG GGTACTTGGT GAATGGTATG CTTCCGTACT GAAGCTGTGG TGATAAAATA
  661 TCCCAAGCAA ATGGCAGAGG TCCACCCTTG GTGACAGTGA GCTTTACTGT CTGCTCTCCC
  721 TCGTAAGGCT TTCCTTTTCC ATCGCCTTCG ACCTCAAAGT AGTGTCCATT GACCGTGCCT
  781 GACATATAAA CCTTGTANGT CATTTGTTTA GCGATCACAC TCACCATGGT CCCCGCGGCC
  841 GCTGATCCNA GCTTTGTTCT CNAGTTTNCN NANTTCTCTG CTGCANTTNC NGNANCTGAN
  901 GNTNCNANGN TAGCGGATNT GANGGNTCNC TAAACCNGCT CTGCTNATAT AGNNCNNCCA
  961 NCGNANACGN CNTANNGCCN NTTTNNGNNN ANGGGGNNGN ANNNNNNNNN ANNTTTTGNA
1021 AANNNNNNNN NANTTTNGNN GNNNNANNNA NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18281_B0H4c.seq check
>D07039E5_B0H4_6_SV40pro_ori_F_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCTCGGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA
  781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGNCGG CGAATGGGCT GACCGCTTCC
  841 TCGNGCTTTA CGGNATCGCC GCTCCCGATT CNCAGCGCAT CGCNNTCNAT CGCCTTCTTG
  901 ACGANTTCTT CTGANCGGGN NTCNNGGGGT TCGAANTGNN NNANCNAGCG ACGCCNNACC
961 NGNCNTCNCN ANNTTTCNAN TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840. [link to RRC of NBRP]