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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1)

CoralHue™ monomeric Keima-Red (pmKeima-Red-MN1)

Catalog number RDB18281
Resource name CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1)
Alternative name AM-V0093M
Clone info. ・MCS-Fluorescent protein/For mammalian expression of the fluorescent protein or its fusion.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone MN1 (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18281zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18281_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18281 CoralHue(TM) monomeric Keima-Red (pmKeima-Red-MN1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18281_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro, MCS, insert 5'
            Sequence file: RDB18281_B0H4a.seq check
            >D07039E5_B0H4_6_CMV_Forward_A07_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCATTG GATCCTCAGG TACCGGAACT
              121 GCAGCAGAGA ATTCGGGAAA CTCGAGAACA AAGCTTGGAT CAGCGGCCGC GGGGACCATG
              181 GTGAGTGTGA TCGCTAAACA AATGACCTAC AAGGTTTATA TGTCAGGCAC GGTCAATGGA
              241 CACTACTTTG AGGTCGAAGG CGATGGAAAA GGAAAGCCTT ACGAGGGAGA GCAGACAGTA
              301 AAGCTCACTG TCACCAAGGG TGGACCTCTG CCATTTGCTT GGGATATTTT ATCACCACAG
              361 CTTCAGTACG GAAGCATACC ATTCACCAAG TACCCTGAAG ACATCCCTGA TTATTTCAAG
              421 CAGTCATTCC CTGAGGGATA TACATGGGAG AGGAGCATGA ACTTTGAAGA TGGTGCAGTG
              481 TGTACTGTCA GCAATGATTC CAGCATCCAA GGCAACTGTT TCATCTACAA TGTCAAAATC
              541 TCTGGTGAGA ACTTTCCTCC CAATGGACCT GTTATGCAGA AGAAGACACA GGGCTGGGAA
              601 CCCAGCACTG AGCGTCTCTT TGCACGAGAT GGAATGCTGA TAGGAAACGA TTATATGGCT
              661 CTGAAGTTGG AAGGAGGTGG TCACTATTTG TGTGAATTTA AATCTACTTA CAAGGCAAAG
              721 AAGCCTGTGA GGATGCCAGG GCGCCACGAG ATTGACCGCA AACTGGATGT AACCAGTCAC
              781 AACAGGGANN ACACATCTGT TGAGCAGTGT GAAATAGCCA TTGCACGCCA CTCTTTGCTC
              841 GGTTAATCTA GAGGGGGCCG CGANTCTAGA TCATAATCAG CCNTACCACA TTTGNAGANG
              901 NTTTACTNGC TTNAAAAAAC CTNCCNCACC NNCCCCNNAN NNNNAAANNT AAAATGAANG
              961 NNNTTGNTGN TGNNNACNNN NTNANNGNNN NNTATNNNNN NTNNNNANNN ANNNNNNNNN
            1021 N
            //
            Primer: SV40polyA_R2
            Region: insert 3'
            Sequence file: RDB18281_B0H4b.seq check
            >D07039E5_B0H4_6_SV40polyA-R2_A08_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NANNNNNGTT AANAACNANN ATTGCATTCN TTTTATGTTT
               61 CNGGTTCNGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
              121 ATGGCTGATT ATGATCTAGA GTCGCGGCCC CCTCTAGATT AACCGAGCAA AGAGTGGCGT
              181 GCAATGGCTA TTTCACACTG CTCAACAGAT GTGTAATCCC TGTTGTGACT GGTTACATCC
              241 AGTTTGCGGT CAATCTCGTG GCGCCCTGGC ATCCTCACAG GCTTCTTTGC CTTGTAAGTA
              301 GATTTAAATT CACACAAATA GTGACCACCT CCTTCCAACT TCAGAGCCAT ATAATCGTTT
              361 CCTATCAGCA TTCCATCTCG TGCAAAGAGA CGCTCAGTGC TGGGTTCCCA GCCCTGTGTC
              421 TTCTTCTGCA TAACAGGTCC ATTGGGAGGA AAGTTCTCAC CAGAGATTTT GACATTGTAG
              481 ATGAAACAGT TGCCTTGGAT GCTGGAATCA TTGCTGACAG TACACACTGC ACCATCTTCA
              541 AAGTTCATGC TCCTCTCCCA TGTATATCCC TCAGGGAATG ACTGCTTGAA ATAATCAGGG
              601 ATGTCTTCAG GGTACTTGGT GAATGGTATG CTTCCGTACT GAAGCTGTGG TGATAAAATA
              661 TCCCAAGCAA ATGGCAGAGG TCCACCCTTG GTGACAGTGA GCTTTACTGT CTGCTCTCCC
              721 TCGTAAGGCT TTCCTTTTCC ATCGCCTTCG ACCTCAAAGT AGTGTCCATT GACCGTGCCT
              781 GACATATAAA CCTTGTANGT CATTTGTTTA GCGATCACAC TCACCATGGT CCCCGCGGCC
              841 GCTGATCCNA GCTTTGTTCT CNAGTTTNCN NANTTCTCTG CTGCANTTNC NGNANCTGAN
              901 GNTNCNANGN TAGCGGATNT GANGGNTCNC TAAACCNGCT CTGCTNATAT AGNNCNNCCA
              961 NCGNANACGN CNTANNGCCN NTTTNNGNNN ANGGGGNNGN ANNNNNNNNN ANNTTTTGNA
            1021 AANNNNNNNN NANTTTNGNN GNNNNANNNA NNNNNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori, NeoR_KanR
            Sequence file: RDB18281_B0H4c.seq check
            >D07039E5_B0H4_6_SV40pro_ori_F_A09_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNCTCGGCCT CTGANCTATT CNNGAAGTAG TGAGGAGGCT
               61 TTTTTGGAGG CCTAGGCTTT TGCAAAGATC ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
              121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
              181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
              241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
              301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
              361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
              421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
              481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
              541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
              601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
              661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
              721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA
              781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAN AGCTTGNCGG CGAATGGGCT GACCGCTTCC
              841 TCGNGCTTTA CGGNATCGCC GCTCCCGATT CNCAGCGCAT CGCNNTCNAT CGCCTTCTTG
              901 ACGANTTCTT CTGANCGGGN NTCNNGGGGT TCGAANTGNN NNANCNAGCG ACGCCNNACC
            961 NGNCNTCNCN ANNTTTCNAN TNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl