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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1)

CoralHue™ monomeric Keima-Red (pmKeima-Red-S1)

Catalog number RDB18280
Resource name CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1)
Alternative name AM-V0091M
Clone info. ・For subcloning.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Montipora. sp red fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18280zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18280_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18280 CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18280_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18280_B0H4a.seq check
            >D07039E4_B0H4_12_lacIpro-B_F_E03_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNC GNNNCNGTGN NCGAGGAAGC GGAAGAGCGC CCAATACGCA
               61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
              121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
              181 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
              241 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT CGAGCTCGGT ACCCGGGGAT
              301 CCCCACCATG GTGAGTGTGA TCGCTAAACA AATGACCTAC AAGGTTTATA TGTCAGGCAC
              361 GGTCAATGGA CACTACTTTG AGGTCGAAGG CGATGGAAAA GGAAAGCCTT ACGAGGGAGA
              421 GCAGACAGTA AAGCTCACTG TCACCAAGGG TGGACCTCTG CCATTTGCTT GGGATATTTT
              481 ATCACCACAG CTTCAGTACG GAAGCATACC ATTCACCAAG TACCCTGAAG ACATCCCTGA
              541 TTATTTCAAG CAGTCATTCC CTGAGGGATA TACATGGGAG AGGAGCATGA ACTTTGAAGA
              601 TGGTGCAGTG TGTACTGTCA GCAATGATTC CAGCATCCAA GGCAACTGTT TCATCTACAA
              661 TGTCAAAATC TCTGGTGAGA ACTTTCCTCC CAATGGACCT GTTATGCAGA AGAAGACACA
              721 GGGCTGGGAA CCCAGCACTG ANCGTCTCTT TGCACGANAT GGAATGCTGA TAGGAAACGA
              781 TTATATGGCT CTGAANTTGG AANGANGTGG TCACTATTTG TGTGAATTTA AATCTACTTA
              841 CAAGGCAAAG AANCCTGNNA NGANGNCAGG GCGCCACGAN ANTGANNGCA AANNGGNTGT
              901 NACCAGTCNC NACANGGNNT ACNCATCTGN NNNNCANNNN NAAATANNCN TNGNNNGNNN
            961 NNNNTTGNNN NGNNNNNNNN NN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert
            Sequence file: RDB18280_B0H4b.seq check
            >D07039E4_B0H4_12_pUC-M13_-100_F03_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGG GNANGCCNGG GTTTTCCCNG TCACGACGTT GTAAAACGAC
               61 GGCCAGTGCC AAGCTTGCAT GCCTGCAGTT AACCGAGCAA AGAGTGGCGT GCAATGGCTA
              121 TTTCACACTG CTCAACAGAT GTGTAATCCC TGTTGTGACT GGTTACATCC AGTTTGCGGT
              181 CAATCTCGTG GCGCCCTGGC ATCCTCACAG GCTTCTTTGC CTTGTAAGTA GATTTAAATT
              241 CACACAAATA GTGACCACCT CCTTCCAACT TCAGAGCCAT ATAATCGTTT CCTATCAGCA
              301 TTCCATCTCG TGCAAAGAGA CGCTCAGTGC TGGGTTCCCA GCCCTGTGTC TTCTTCTGCA
              361 TAACAGGTCC ATTGGGAGGA AAGTTCTCAC CAGAGATTTT GACATTGTAG ATGAAACAGT
              421 TGCCTTGGAT GCTGGAATCA TTGCTGACAG TACACACTGC ACCATCTTCA AAGTTCATGC
              481 TCCTCTCCCA TGTATATCCC TCAGGGAATG ACTGCTTGAA ATAATCAGGG ATGTCTTCAG
              541 GGTACTTGGT GAATGGTATG CTTCCGTACT GAAGCTGTGG TGATAAAATA TCCCAAGCAA
              601 ATGGCAGAGG TCCACCCTTG GTGACAGTGA GCTTTACTGT CTGCTCTCCC TCGTAAGGCT
              661 TTCCTTTTCC ATCGCCTTCG ACCTCAAAGT AGTGTCCATT GACCGTGCCT GACATATAAA
              721 CCTTGTAGGT CATTTGTTTA GCGATCACAC TCACCATGGT GGGGATCCCC GGGTACCGAG
              781 CTCGAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
              841 TCCACACAAC ATACGANCCG GAANCATAAA GTGTAAAGCC TGGGGNGCCN AANGANTGAN
              901 CTAACTCACA NNAATTGCGT TGCGCTCNCN GCCNNCNTTC CANNNNGNNA ANCNNNCNNG
            961 NNNNCNNCNT TNNNGNANNN NNCNNNCNNN NNNGNNNNAN NNNNNTNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl