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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1)

CoralHue™ monomeric Keima-Red (pmKeima-Red-S1)

Catalog number RDB18280
Resource name CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1)
Alternative name AM-V0091M
Clone info. ・For subcloning.
・Monomeric Keima-Red exhibits an extremely large Stokes shift(180 nm).
・Ex/Em(nm): 440/620
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Montipora. sp red fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581, 2006) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18280 CoralHue(TM) monomeric Keima-Red (pmKeima-Red-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB18280zzk02.seq provided by the depositor
Remarks, protocol and/or map (png) RDB18280_map.png

Original reference

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18280_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18280_B0H4a.seq check
>D07039E4_B0H4_12_lacIpro-B_F_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNC GNNNCNGTGN NCGAGGAAGC GGAAGAGCGC CCAATACGCA
   61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
  121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
  181 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  241 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT CGAGCTCGGT ACCCGGGGAT
  301 CCCCACCATG GTGAGTGTGA TCGCTAAACA AATGACCTAC AAGGTTTATA TGTCAGGCAC
  361 GGTCAATGGA CACTACTTTG AGGTCGAAGG CGATGGAAAA GGAAAGCCTT ACGAGGGAGA
  421 GCAGACAGTA AAGCTCACTG TCACCAAGGG TGGACCTCTG CCATTTGCTT GGGATATTTT
  481 ATCACCACAG CTTCAGTACG GAAGCATACC ATTCACCAAG TACCCTGAAG ACATCCCTGA
  541 TTATTTCAAG CAGTCATTCC CTGAGGGATA TACATGGGAG AGGAGCATGA ACTTTGAAGA
  601 TGGTGCAGTG TGTACTGTCA GCAATGATTC CAGCATCCAA GGCAACTGTT TCATCTACAA
  661 TGTCAAAATC TCTGGTGAGA ACTTTCCTCC CAATGGACCT GTTATGCAGA AGAAGACACA
  721 GGGCTGGGAA CCCAGCACTG ANCGTCTCTT TGCACGANAT GGAATGCTGA TAGGAAACGA
  781 TTATATGGCT CTGAANTTGG AANGANGTGG TCACTATTTG TGTGAATTTA AATCTACTTA
  841 CAAGGCAAAG AANCCTGNNA NGANGNCAGG GCGCCACGAN ANTGANNGCA AANNGGNTGT
  901 NACCAGTCNC NACANGGNNT ACNCATCTGN NNNNCANNNN NAAATANNCN TNGNNNGNNN
961 NNNNTTGNNN NGNNNNNNNN NN
//
Primer: pUC-M13_-100
Region: MCS, insert
Sequence file: RDB18280_B0H4b.seq check
>D07039E4_B0H4_12_pUC-M13_-100_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GNANGCCNGG GTTTTCCCNG TCACGACGTT GTAAAACGAC
   61 GGCCAGTGCC AAGCTTGCAT GCCTGCAGTT AACCGAGCAA AGAGTGGCGT GCAATGGCTA
  121 TTTCACACTG CTCAACAGAT GTGTAATCCC TGTTGTGACT GGTTACATCC AGTTTGCGGT
  181 CAATCTCGTG GCGCCCTGGC ATCCTCACAG GCTTCTTTGC CTTGTAAGTA GATTTAAATT
  241 CACACAAATA GTGACCACCT CCTTCCAACT TCAGAGCCAT ATAATCGTTT CCTATCAGCA
  301 TTCCATCTCG TGCAAAGAGA CGCTCAGTGC TGGGTTCCCA GCCCTGTGTC TTCTTCTGCA
  361 TAACAGGTCC ATTGGGAGGA AAGTTCTCAC CAGAGATTTT GACATTGTAG ATGAAACAGT
  421 TGCCTTGGAT GCTGGAATCA TTGCTGACAG TACACACTGC ACCATCTTCA AAGTTCATGC
  481 TCCTCTCCCA TGTATATCCC TCAGGGAATG ACTGCTTGAA ATAATCAGGG ATGTCTTCAG
  541 GGTACTTGGT GAATGGTATG CTTCCGTACT GAAGCTGTGG TGATAAAATA TCCCAAGCAA
  601 ATGGCAGAGG TCCACCCTTG GTGACAGTGA GCTTTACTGT CTGCTCTCCC TCGTAAGGCT
  661 TTCCTTTTCC ATCGCCTTCG ACCTCAAAGT AGTGTCCATT GACCGTGCCT GACATATAAA
  721 CCTTGTAGGT CATTTGTTTA GCGATCACAC TCACCATGGT GGGGATCCCC GGGTACCGAG
  781 CTCGAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
  841 TCCACACAAC ATACGANCCG GAANCATAAA GTGTAAAGCC TGGGGNGCCN AANGANTGAN
  901 CTAACTCACA NNAATTGCGT TGCGCTCNCN GCCNNCNTTC CANNNNGNNA ANCNNNCNNG
961 NNNNCNNCNT TNNNGNANNN NNCNNNCNNN NNNGNNNNAN NNNNNTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kogure, T., A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat. Biotechnol. 24 (5): 577-581 (2006). PMID 16648840.

2021.10.18

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