Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1)

CoralHue™ humanized Kikume Green-Red 1 (phKikGR1-S1)

Catalog number RDB18275
Resource name CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1)
Alternative name AM-V0084M
Clone info. ・For subcloning.
・The codons are humanized.
・Kikume Green-Red1 is bright green fluorescent protein that can be irreversibly converted to red by UV or violet light(350-410nm).
・Ex/Em(nm): 507/517(Green),583/593(Red)
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Favia favus Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18275 CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB18275zzk02.seq provided by the depositor
Remarks, protocol and/or map (png) RDB18275_map.png

Original reference

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18275_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18275_B0H4a.seq check
>D07039D8_B0H4_15_lacIpro-B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCGNN NCGNNTCNGT GNGCGAGGAA GCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
  121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
  181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG
  301 ATCCCCACCA TGGTGAGCGT GATCACCAGC GAGATGAAGA TCGAGCTGAG GATGGAGGGC
  361 GCCGTGAACG GCCACAAGTT CGTGATCACC GGCAAGGGCA GCGGCCAGCC CTTCGAGGGC
  421 ATCCAGAACG TGGACCTGAC CGTGATCGAG GGCGGCCCCC TGCCCTTCGC CTTCGACATC
  481 CTGACCACCG CCTTCCACTA CGGCAACAGG GTGTTCGTGG AGTACCCCGA GGAGATCGTG
  541 GACTACTTCA AGCAGAGCTT CCCCGAGGGC TACAGCTGGG AGAGGAGCAT GAGCTACGAG
  601 GACGGCGGCA TCTGCCTGGC CACCAACAAC ATCACCATGA AGAAGGACGG CAGCAACTGC
  661 TTCGTGAACG AGATCAGGTT CGACGGCGTG AACTTCCCCG CCAACGGCCC CGTGATGCAG
  721 AGGAAGANCG TGAAGTGGGA GCCCAGCACC GAGAAGANGT ACGTGAGGGA CGGCGTGCTG
  781 AANGGCGACG TGAACATGGC CCTGCTGCTC CAGGGCGGCG GCCACTACAG GTGCGACTTC
  841 NNGNNCACCT ACAANGNCAA GAANGTGGTG CAGCTGCCCG ANTACCACTT CNNNGANNAC
  901 CAGATGGANN TCACCAGCCA CGACAAGGNN TACNACNANN NNGAANCTGN NNNANCACGC
961 CNNNGNCNNC AGCNGNCCNG NCNNNGNNNG GNCNNNNNNN NNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18275_B0H4b.seq check
>D07039D8_B0H4_15_pUC-M13_-100_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNANGCNNG GNNTTTCCCN GTCNCGACGT TGTAAAACGA
   61 CGGCCAGTGC CAAGCTTGCA TGCCTGCAGT TACTTGGCCA GCCTGGGCAG GCCGCTGTGG
  121 GCCTTGGCGT GCTCGTACAG CTTCACCTTG TTGTAGTCCT TGTCGTGGCT GGTGATCTCC
  181 ATCTGGTGGT CCACGAAGTG GTAGTCGGGC AGCTGCACCA CCTTCTTGGC CTTGTAGGTG
  241 GTCCTGAAGT CGCACCTGTA GTGGCCGCCG CCCTGGAGCA GCAGGGCCAT GTTCACGTCG
  301 CCCTTCAGCA CGCCGTCCCT CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCACGGTC
  361 TTCCTCTGCA TCACGGGGCC GTTGGCGGGG AAGTTCACGC CGTCGAACCT GATCTCGTTC
  421 ACGAAGCAGT TGCTGCCGTC CTTCTTCATG GTGATGTTGT TGGTGGCCAG GCAGATGCCG
  481 CCGTCCTCGT AGCTCATGCT CCTCTCCCAG CTGTAGCCCT CGGGGAAGCT CTGCTTGAAG
  541 TAGTCCACGA TCTCCTCGGG GTACTCCACG AACACCCTGT TGCCGTAGTG GAAGGCGGTG
  601 GTCAGGATGT CGAAGGCGAA NGGCAGGGGG CCGCCCTCGA TCACGGTCAG GTCCACGTTC
  661 TGGATGCCCT CGAAGGGCTG GCCGCTGCCC TTGCCGGTGA NCACGAACTT GTGGCCGTTC
  721 ACGGCGCCCT CCATCCTCAG CTCGATCTTC ATCTCGCTGG TGATCACGCT CACCATGGTG
  781 GGGATCCCCG GGTACCGAGC TCGAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
  841 NTTGTTATCC GCTCACAATT CCNCACAACA TACGANCCGG AANCATAAAG TGTAAAGCCT
  901 GGGGNGNCTA ATGANTGANC NAACTCACAT TAATTGCGTT GCGCNNNCTG NCNGCTTNNC
  961 ANNNNGNNAA CCNNNCNNNN CANCNNCNTT AANGAANNNN GNCNACGNNN NGGNNNNNNN
1021 NGNNTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

2021.10.18

GNP_filter3_RDBDEP_html_210618.pl