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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1)

CoralHue™ humanized Kikume Green-Red 1 (phKikGR1-S1)

Catalog number RDB18275
Resource name CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1)
Alternative name AM-V0084M
Clone info. ・For subcloning.
・The codons are humanized.
・Kikume Green-Red1 is bright green fluorescent protein that can be irreversibly converted to red by UV or violet light(350-410nm).
・Ex/Em(nm): 507/517(Green),583/593(Red)
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Favia favus Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Favia favus Green-Red (KikGR) fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18275zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18275_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18275 CoralHue(TM) humanized Kikume Green-Red 1 (phKikGR1-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18275_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18275_B0H4a.seq check
            >D07039D8_B0H4_15_lacIpro-B_F_C03_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNCGNN NCGNNTCNGT GNGCGAGGAA GCGGAAGAGC GCCCAATACG
               61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
              121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
              181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
              241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG
              301 ATCCCCACCA TGGTGAGCGT GATCACCAGC GAGATGAAGA TCGAGCTGAG GATGGAGGGC
              361 GCCGTGAACG GCCACAAGTT CGTGATCACC GGCAAGGGCA GCGGCCAGCC CTTCGAGGGC
              421 ATCCAGAACG TGGACCTGAC CGTGATCGAG GGCGGCCCCC TGCCCTTCGC CTTCGACATC
              481 CTGACCACCG CCTTCCACTA CGGCAACAGG GTGTTCGTGG AGTACCCCGA GGAGATCGTG
              541 GACTACTTCA AGCAGAGCTT CCCCGAGGGC TACAGCTGGG AGAGGAGCAT GAGCTACGAG
              601 GACGGCGGCA TCTGCCTGGC CACCAACAAC ATCACCATGA AGAAGGACGG CAGCAACTGC
              661 TTCGTGAACG AGATCAGGTT CGACGGCGTG AACTTCCCCG CCAACGGCCC CGTGATGCAG
              721 AGGAAGANCG TGAAGTGGGA GCCCAGCACC GAGAAGANGT ACGTGAGGGA CGGCGTGCTG
              781 AANGGCGACG TGAACATGGC CCTGCTGCTC CAGGGCGGCG GCCACTACAG GTGCGACTTC
              841 NNGNNCACCT ACAANGNCAA GAANGTGGTG CAGCTGCCCG ANTACCACTT CNNNGANNAC
              901 CAGATGGANN TCACCAGCCA CGACAAGGNN TACNACNANN NNGAANCTGN NNNANCACGC
            961 CNNNGNCNNC AGCNGNCCNG NCNNNGNNNG GNCNNNNNNN NNN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18275_B0H4b.seq check
            >D07039D8_B0H4_15_pUC-M13_-100_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GGNANGCNNG GNNTTTCCCN GTCNCGACGT TGTAAAACGA
               61 CGGCCAGTGC CAAGCTTGCA TGCCTGCAGT TACTTGGCCA GCCTGGGCAG GCCGCTGTGG
              121 GCCTTGGCGT GCTCGTACAG CTTCACCTTG TTGTAGTCCT TGTCGTGGCT GGTGATCTCC
              181 ATCTGGTGGT CCACGAAGTG GTAGTCGGGC AGCTGCACCA CCTTCTTGGC CTTGTAGGTG
              241 GTCCTGAAGT CGCACCTGTA GTGGCCGCCG CCCTGGAGCA GCAGGGCCAT GTTCACGTCG
              301 CCCTTCAGCA CGCCGTCCCT CACGTACATC TTCTCGGTGC TGGGCTCCCA CTTCACGGTC
              361 TTCCTCTGCA TCACGGGGCC GTTGGCGGGG AAGTTCACGC CGTCGAACCT GATCTCGTTC
              421 ACGAAGCAGT TGCTGCCGTC CTTCTTCATG GTGATGTTGT TGGTGGCCAG GCAGATGCCG
              481 CCGTCCTCGT AGCTCATGCT CCTCTCCCAG CTGTAGCCCT CGGGGAAGCT CTGCTTGAAG
              541 TAGTCCACGA TCTCCTCGGG GTACTCCACG AACACCCTGT TGCCGTAGTG GAAGGCGGTG
              601 GTCAGGATGT CGAAGGCGAA NGGCAGGGGG CCGCCCTCGA TCACGGTCAG GTCCACGTTC
              661 TGGATGCCCT CGAAGGGCTG GCCGCTGCCC TTGCCGGTGA NCACGAACTT GTGGCCGTTC
              721 ACGGCGCCCT CCATCCTCAG CTCGATCTTC ATCTCGCTGG TGATCACGCT CACCATGGTG
              781 GGGATCCCCG GGTACCGAGC TCGAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
              841 NTTGTTATCC GCTCACAATT CCNCACAACA TACGANCCGG AANCATAAAG TGTAAAGCCT
              901 GGGGNGNCTA ATGANTGANC NAACTCACAT TAATTGCGTT GCGCNNNCTG NCNGCTTNNC
              961 ANNNNGNNAA CCNNNCNNNN CANCNNCNTT AANGAANNNN GNCNACGNNN NGGNNNNNNN
            1021 NGNNTNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl