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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1)

CoralHue™ Kikume Green-Red 1 (pKikGR1-S1)

Catalog number RDB18272
Resource name CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1)
Alternative name AM-V0081M
Clone info. ・For subcloning.
・Kikume Green-Red1 is bright green fluorescent protein that can be irreversibly converted to red by UV or violet light(350-410nm).
・Ex/Em(nm): 507/517(Green),583/593(Red)
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Favia favus Green-Red (KikGR) fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank

External Database
Favia favus Green-Red (KikGR) fluorescent protein

            Reference sequence
              
             
            Sequence (full) RDB18272zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18272_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238, 2005)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18272 CoralHue(TM) Kikume Green-Red 1 (pKikGR1-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18272_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: MCS, insert 5'
            Sequence file: RDB18272_B0H4a.seq check
            >D07039D5_B0H4_14_lacIpro-B_F_E06_15_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN CGNNNCNGTG AGCGNNGANC GGAAGANCGC CCAATACGCA
               61 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG
              121 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC
              181 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
              241 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT CGAGCTCGGT ACCCGGGGAT
              301 CCCCACCATG GTGAGTGTGA TTACATCAGA AATGAAGATC GAGCTGCGTA TGGAAGGCGC
              361 TGTAAACGGG CACAAGTTCG TGATTACAGG GAAAGGAAGT GGCCAGCCTT TCGAGGGAAT
              421 ACAGAATGTG GACCTGACAG TCATAGAGGG CGGACCTCTT CCTTTTGCTT TCGATATCCT
              481 GACAACAGCA TTCCATTACG GCAACCGGGT ATTTGTCGAA TACCCAGAAG AAATAGTAGA
              541 CTACTTCAAG CAGTCGTTTC CTGAGGGTTA TTCTTGGGAA CGAAGCATGA GTTACGAAGA
              601 CGGGGGAATT TGCCTCGCCA CAAACAATAT AACGATGAAG AAAGACGGCA GCAACTGTTT
              661 TGTCAATGAA ATTCGATTTG ATGGTGTGAA CTTTCCTGCC AATGGTCCAG TTATGCAGAG
              721 GAAGANCGTC AAATGGGAGC CATCCACTGA GAAAATGTAT GTGCGTGATG GANTGCTGAA
              781 NGGTGANGTA AACATGGCTC TGTTGCTTCN AGGANGTGGC CATTACCGAT GTGACTTCAG
              841 AACTACTTAC NAAGCAAAGA ANGNTGTCCA GTNGNNNGAN NATCACTTCN NGGATCATCN
              901 AANNNNNATN ACAAGCNNNG ANNAGGNNNA NNACNANGNT TNANCTGNNN NANCNCGNNN
            961 AAGNNNNNNN CNNGNNNNNN NNNN
            //
            Primer: pUC-M13_-100
            Region: MCS, insert 3'
            Sequence file: RDB18272_B0H4b.seq check
            >D07039D5_B0H4_14_pUC-M13_-100_F06_18_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNANGCCNG GNNTTTCCCN GTCNCGACGT TGTAAAACGA
               61 CGGCCAGTGC CAAGCTTGCA TGCCTGCAGT TACTTGGCCA GCCTTGGCAG CCCGGAATGA
              121 GCTTTAGCGT GCTCATACAG CTTAACCTTG TTGTAATCCT TGTCATGGCT TGTTATCTCC
              181 ATTTGATGAT CCACGAAGTG ATAGTCTGGC AACTGGACAA CCTTCTTTGC TTTGTAAGTA
              241 GTTCTGAAGT CACATCGGTA ATGGCCACCT CCTTGAAGCA ACAGAGCCAT GTTTACATCA
              301 CCCTTCAGCA CTCCATCACG CACATACATT TTCTCAGTGG ATGGCTCCCA TTTGACGGTC
              361 TTCCTCTGCA TAACTGGACC ATTGGCAGGA AAGTTCACAC CATCAAATCG AATTTCATTG
              421 ACAAAACAGT TGCTGCCGTC TTTCTTCATC GTTATATTGT TTGTGGCGAG GCAAATTCCC
              481 CCGTCTTCGT AACTCATGCT TCGTTCCCAA GAATAACCCT CAGGAAACGA CTGCTTGAAG
              541 TAGTCTACTA TTTCTTCTGG GTATTCGACA AATACCCGGT TGCCGTAATG GAATGCTGTT
              601 GTCAGGANAT CGAAAGCAAA AGGAAGAGGT CCGCCCTCTA TGANTGTCAN GTCCACATTC
              661 TGTATTCCCT CNAAAGGNTG GNCACTTCCT TTCCCTGNAA TCNNNAANTT GTGNCCGNNT
              721 ANAGCGNCTT CCNTACNCAG CTCGNTCTTC ATTTCTGANG TANTCACACT CACCATGGTG
              781 GGGNNNCCCN GNTANCGANC TCGAANTCNN ANNCNNGGTC NTANCTGTTT NNNGNGNGAA
              841 ANNGNNATCC NNTCNCNNTN NCNCACNNNN TANNANCNNG NNNNATAAAN NGNAAANNNN
            901 NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tsutsui, H., Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6 (3): 233-238 (2005). PMID 15731765.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl