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CoralHue(TM) Dronpa-Green 1 (pDG1-S1)

CoralHue™ Dronpa-Green 1 (pDG1-S1)

Catalog number RDB18267
Resource name CoralHue(TM) Dronpa-Green 1 (pDG1-S1)
Alternative name AM-V0071M
Clone info. ・For subcloning.
・Dronpa-Green1 is a monomeric Green fluorescent protein that is photochromic, its fluorescence can be reversibly turned on (400 nm) and "off (500 nm).
Ex/Em (nm): 503/518
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Echinophyllia sp. green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Ando, R. et al., Science 306 (5700): 1370-1373, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18267 CoralHue(TM) Dronpa-Green 1 (pDG1-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence provided by the depositor RDB18267zzk01.seq
Electronic file Map RDB18267_map.png

Original reference

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18267_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18267_B0H4a.seq check
>D07039C9_B0H4_3_lacIpro-B_F_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCAN CGNNTCNGTG NNCGAGGAAN CGGAANANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
  121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
  181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA
  301 TCCCCACCAT GGTGAGTGTG ATTAAACCAG ACATGAAGAT CAAGCTGCGT ATGGAAGGCG
  361 CTGTAAATGG ACACCCGTTC GCGATTGAAG GAGTTGGCCT TGGGAAGCCT TTCGAGGGAA
  421 AACAGAGTAT GGACCTTAAA GTCAAAGAAG GCGGACCTCT GCCTTTCGCC TATGACATCT
  481 TGACAACTGT GTTCTGTTAC GGCAACAGGG TATTCGCCAA ATACCCAGAA AATATAGTAG
  541 ACTATTTCAA GCAGTCGTTT CCTGAGGGCT ACTCTTGGGA ACGAAGCATG AATTACGAAG
  601 ACGGGGGCAT TTGTAACGCG ACAAACGACA TAACCCTGGA TGGTGACTGT TATATCTATG
  661 AAATTCGATT TGATGGTGTG AACTTTCCTG CNAATGGTCC AGTTATGCAG AAGANGACTG
  721 TGAAATGGGA GCCATCNACT GANAAANTGT ATGTGCGTGA TGGANTGCTG AAGGGTGATG
  781 TTAACATGGC TCTGTCGCTT GAANGANGTG GCCATTACNG ATGTGACTTC AAAACTACTT
  841 ATAAAGCTNA NAANGNTGTC CAGTTGNNAG ANTATCACTT TGTGGANCAC NNCATNNAGA
  901 NTAAAAGCNN CNNNAAAGAN TANNNTNANG NTNANTCTNN NTNANCANNN NNAANCNNNN
961 NNNNNN
//
Primer: MBL_S1-rev1
Region: MCS, insert 3'
Sequence file: RDB18267_B0H4b.seq check
>D07039C9_B0H4_3_MBL_S1-rev1_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNGNNCGG NGCGGGCCTC TTCGCTATTA CGCCNGCTGG
   61 CGAAAGGGGG ANGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC
  121 GACGTTGTAA AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGTTACTT GGCCTGCCTC
  181 GGCAGCTCAG AATGCGCTTC GGCGTGCTCA TGCAGATTAA CATTACTGTA ATCTTTGTCG
  241 TGGCTTTTAA TCTCAATGTG GTGGTCCACA AAGTGATAGT CTGGCAACTG GACAACCTTC
  301 TTAGCTTTAT AAGTAGTTTT GAAGTCACAT CGGTAATGGC CACCTCCTTC AAGCGACAGA
  361 GCCATGTTAA CATCACCCTT CAGCACTCCA TCACGCACAT ACAATTTCTC AGTGGATGGC
  421 TCCCATTTCA CAGTCCTCTT CTGCATAACT GGACCATTGG CAGGAAAGTT CACACCATCA
  481 AATCGAATTT CATAGATATA ACAGTCACCA TCCAGGGTTA TGTCGTTTGT CGCGTTACAA
  541 ATGCCCCCGT CTTCGTAATT CATGCTTCGT TCCCAAGAGT AGCCCTCAGG AAACGACTGC
  601 TTGAAATAGT CTACTATATT TTCTGGGTAT TTGGCGAATA CCCTGTTGCC GTAACAGAAC
  661 ACAGTTGTCA AGATGTCATA GGCGAAAGGC AGANGTCCGC CTTCTTTGAC TTTAANGTCC
  721 ATACTCTGTT TTCCCTCGAA AGGCTTCCCA AGGCCAACTC CTTCAATCGC GAACGGGTGT
  781 CCATTTACAG CGCCTTCCAT ACGCAGCTTG ATCTTCATGT CTGGTTTAAT CACACTCACC
  841 ATGGNGGGGN TCCCCNGGNA NCGANCTCGA ATTCNTAATC ATGGNCATAG CNNNTTCCTG
  901 NGNGAAANTG NNATCCNNNN NCNANTNNNC ANNACATACN NNCCNGNNNN ATAANNNGNN
961 ANNNNNNNNG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670.

2020.12.28

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