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icon below as well as clone information before placing your order.
CoralHue™ Dronpa-Green 1 (pDG1-S1)
| Catalog number | RDB18267 |
|---|---|
| Resource name | CoralHue(TM) Dronpa-Green 1 (pDG1-S1) |
| Alternative name | AM-V0071M |
| Clone info. | ・For subcloning. ・Dronpa-Green1 is a monomeric Green fluorescent protein that is photochromic, its fluorescence can be reversibly turned on (400 nm) and "off (500 nm). Ex/Em (nm): 503/518 |
| Vector backbone | S1 (plasmid) |
| Size of vector backbone | 2.7 kb |
| Selectable markers | Ampicillin |
| Gene/insert name | Echinophyllia sp. green fluorescent protein cDNA |
| Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Ando, R. et al., Science 306 (5700): 1370-1373, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB18267 | CoralHue(TM) Dronpa-Green 1 (pDG1-S1) | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Nucleotide sequence provided by the depositor RDB18267zzk01.seq |
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| Electronic file | Map RDB18267_map.png
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Original reference
| original | Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Fluorescent protein resources (English text) |
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| Featured content | Fluorescent protein resources (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18267_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18267_B0H4a.seq
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>D07039C9_B0H4_3_lacIpro-B_F_F09_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNGCAN CGNNTCNGTG NNCGAGGAAN CGGAANANCG CCCAATACGC 61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA 301 TCCCCACCAT GGTGAGTGTG ATTAAACCAG ACATGAAGAT CAAGCTGCGT ATGGAAGGCG 361 CTGTAAATGG ACACCCGTTC GCGATTGAAG GAGTTGGCCT TGGGAAGCCT TTCGAGGGAA 421 AACAGAGTAT GGACCTTAAA GTCAAAGAAG GCGGACCTCT GCCTTTCGCC TATGACATCT 481 TGACAACTGT GTTCTGTTAC GGCAACAGGG TATTCGCCAA ATACCCAGAA AATATAGTAG 541 ACTATTTCAA GCAGTCGTTT CCTGAGGGCT ACTCTTGGGA ACGAAGCATG AATTACGAAG 601 ACGGGGGCAT TTGTAACGCG ACAAACGACA TAACCCTGGA TGGTGACTGT TATATCTATG 661 AAATTCGATT TGATGGTGTG AACTTTCCTG CNAATGGTCC AGTTATGCAG AAGANGACTG 721 TGAAATGGGA GCCATCNACT GANAAANTGT ATGTGCGTGA TGGANTGCTG AAGGGTGATG 781 TTAACATGGC TCTGTCGCTT GAANGANGTG GCCATTACNG ATGTGACTTC AAAACTACTT 841 ATAAAGCTNA NAANGNTGTC CAGTTGNNAG ANTATCACTT TGTGGANCAC NNCATNNAGA 901 NTAAAAGCNN CNNNAAAGAN TANNNTNANG NTNANTCTNN NTNANCANNN NNAANCNNNN 961 NNNNNN // |
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Primer: MBL_S1-rev1 Region: MCS, insert 3' Sequence file: RDB18267_B0H4b.seq
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>D07039C9_B0H4_3_MBL_S1-rev1_E09_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGNGNNCGG NGCGGGCCTC TTCGCTATTA CGCCNGCTGG 61 CGAAAGGGGG ANGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC 121 GACGTTGTAA AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGTTACTT GGCCTGCCTC 181 GGCAGCTCAG AATGCGCTTC GGCGTGCTCA TGCAGATTAA CATTACTGTA ATCTTTGTCG 241 TGGCTTTTAA TCTCAATGTG GTGGTCCACA AAGTGATAGT CTGGCAACTG GACAACCTTC 301 TTAGCTTTAT AAGTAGTTTT GAAGTCACAT CGGTAATGGC CACCTCCTTC AAGCGACAGA 361 GCCATGTTAA CATCACCCTT CAGCACTCCA TCACGCACAT ACAATTTCTC AGTGGATGGC 421 TCCCATTTCA CAGTCCTCTT CTGCATAACT GGACCATTGG CAGGAAAGTT CACACCATCA 481 AATCGAATTT CATAGATATA ACAGTCACCA TCCAGGGTTA TGTCGTTTGT CGCGTTACAA 541 ATGCCCCCGT CTTCGTAATT CATGCTTCGT TCCCAAGAGT AGCCCTCAGG AAACGACTGC 601 TTGAAATAGT CTACTATATT TTCTGGGTAT TTGGCGAATA CCCTGTTGCC GTAACAGAAC 661 ACAGTTGTCA AGATGTCATA GGCGAAAGGC AGANGTCCGC CTTCTTTGAC TTTAANGTCC 721 ATACTCTGTT TTCCCTCGAA AGGCTTCCCA AGGCCAACTC CTTCAATCGC GAACGGGTGT 781 CCATTTACAG CGCCTTCCAT ACGCAGCTTG ATCTTCATGT CTGGTTTAAT CACACTCACC 841 ATGGNGGGGN TCCCCNGGNA NCGANCTCGA ATTCNTAATC ATGGNCATAG CNNNTTCCTG 901 NGNGAAANTG NNATCCNNNN NCNANTNNNC ANNACATACN NNCCNGNNNN ATAANNNGNN 961 ANNNNNNNNG NNNNNNNNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. |
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2020.12.28
GNP_filter3_RDBDEP_html_201222.pl