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CoralHue(TM) Dronpa-Green 1 (pDG1-S1) (#RDB18267)

CoralHue™ Dronpa-Green 1 (pDG1-S1)

Alternative name AM-V0071M
Clone info. ・For subcloning.
・Dronpa-Green1 is a monomeric Green fluorescent protein that is photochromic, its fluorescence can be reversibly turned on (400 nm) and "off (500 nm).
Ex/Em (nm): 503/518
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Echinophyllia sp. green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18267zzk01.seq provided by the depositor
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Ando, R. et al., Science 306 (5700): 1370-1373, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ando, R. et al., Science 306 (5700): 1370-1373, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

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RDB18267 CoralHue(TM) Dronpa-Green 1 (pDG1-S1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) Dronpa-Green 1 (pDG1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18267).

Reference section:

Ando, R., Mizuno, H., Miyawaki, A., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18267_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18267_B0H4a.seq check
>D07039C9_B0H4_3_lacIpro-B_F_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCAN CGNNTCNGTG NNCGAGGAAN CGGAANANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
  121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
  181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA
  301 TCCCCACCAT GGTGAGTGTG ATTAAACCAG ACATGAAGAT CAAGCTGCGT ATGGAAGGCG
  361 CTGTAAATGG ACACCCGTTC GCGATTGAAG GAGTTGGCCT TGGGAAGCCT TTCGAGGGAA
  421 AACAGAGTAT GGACCTTAAA GTCAAAGAAG GCGGACCTCT GCCTTTCGCC TATGACATCT
  481 TGACAACTGT GTTCTGTTAC GGCAACAGGG TATTCGCCAA ATACCCAGAA AATATAGTAG
  541 ACTATTTCAA GCAGTCGTTT CCTGAGGGCT ACTCTTGGGA ACGAAGCATG AATTACGAAG
  601 ACGGGGGCAT TTGTAACGCG ACAAACGACA TAACCCTGGA TGGTGACTGT TATATCTATG
  661 AAATTCGATT TGATGGTGTG AACTTTCCTG CNAATGGTCC AGTTATGCAG AAGANGACTG
  721 TGAAATGGGA GCCATCNACT GANAAANTGT ATGTGCGTGA TGGANTGCTG AAGGGTGATG
  781 TTAACATGGC TCTGTCGCTT GAANGANGTG GCCATTACNG ATGTGACTTC AAAACTACTT
  841 ATAAAGCTNA NAANGNTGTC CAGTTGNNAG ANTATCACTT TGTGGANCAC NNCATNNAGA
  901 NTAAAAGCNN CNNNAAAGAN TANNNTNANG NTNANTCTNN NTNANCANNN NNAANCNNNN
961 NNNNNN
//
Primer: MBL_S1-rev1 (Pr0941)
Region: MCS, insert 3'
Sequence file: RDB18267_B0H4b.seq check
>D07039C9_B0H4_3_MBL_S1-rev1_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNGNNCGG NGCGGGCCTC TTCGCTATTA CGCCNGCTGG
   61 CGAAAGGGGG ANGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC
  121 GACGTTGTAA AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGTTACTT GGCCTGCCTC
  181 GGCAGCTCAG AATGCGCTTC GGCGTGCTCA TGCAGATTAA CATTACTGTA ATCTTTGTCG
  241 TGGCTTTTAA TCTCAATGTG GTGGTCCACA AAGTGATAGT CTGGCAACTG GACAACCTTC
  301 TTAGCTTTAT AAGTAGTTTT GAAGTCACAT CGGTAATGGC CACCTCCTTC AAGCGACAGA
  361 GCCATGTTAA CATCACCCTT CAGCACTCCA TCACGCACAT ACAATTTCTC AGTGGATGGC
  421 TCCCATTTCA CAGTCCTCTT CTGCATAACT GGACCATTGG CAGGAAAGTT CACACCATCA
  481 AATCGAATTT CATAGATATA ACAGTCACCA TCCAGGGTTA TGTCGTTTGT CGCGTTACAA
  541 ATGCCCCCGT CTTCGTAATT CATGCTTCGT TCCCAAGAGT AGCCCTCAGG AAACGACTGC
  601 TTGAAATAGT CTACTATATT TTCTGGGTAT TTGGCGAATA CCCTGTTGCC GTAACAGAAC
  661 ACAGTTGTCA AGATGTCATA GGCGAAAGGC AGANGTCCGC CTTCTTTGAC TTTAANGTCC
  721 ATACTCTGTT TTCCCTCGAA AGGCTTCCCA AGGCCAACTC CTTCAATCGC GAACGGGTGT
  781 CCATTTACAG CGCCTTCCAT ACGCAGCTTG ATCTTCATGT CTGGTTTAAT CACACTCACC
  841 ATGGNGGGGN TCCCCNGGNA NCGANCTCGA ATTCNTAATC ATGGNCATAG CNNNTTCCTG
  901 NGNGAAANTG NNATCCNNNN NCNANTNNNC ANNACATACN NNCCNGNNNN ATAANNNGNN
961 ANNNNNNNNG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ando, R., Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306 (5700): 1370-1373 (2004). PMID 15550670. [link to RRC of NBRP]