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CoralHue™ Midoriishi-Cyan 1 (pMiCy1-S1)
| Catalog number | RDB18265 |
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| Resource name | CoralHue(TM) Midoriishi-Cyan 1 (pMiCy1-S1) |
| Alternative name | AM-V0061M |
| Clone info. | ・For subcloning. ・Midoriishi-Cyan1 is Cyan fluorescent protein that rapidly mature to form fluorescent dimer. ・Ex/Em (nm): 472/495 |
| Vector backbone | S1 (plasmid) |
| Size of vector backbone | 2.7 kb |
| Selectable markers | Ampicillin |
| Gene/insert name | Acropara sp. cyan fluorescent protein cDNA |
| Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB18265 | CoralHue(TM) Midoriishi-Cyan 1 (pMiCy1-S1) | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Map RDB18265_map.png
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Original reference
| original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Fluorescent protein resources (English text) |
|---|---|
| Featured content | Fluorescent protein resources (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18265_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18265_B0H4a.seq
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>D07039C7_B0H4_1_lacIpro-B_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN ANCGNNTCAG TGANCGNNGA NCGGAAGANC GCCCAATACG 61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG 301 ATCCCCACCA TGGTGTCTTA TTCAAAGCAA GGCATCGCAC AAGAAATGCG GACGAAATAC 361 CGTATGGAAG GCAGTGTCAA TGGCCATGAG TTCACGATCG AAGGTGTAGG AACTGGAAAC 421 CCTTACGAAG GGAAACAGAT GTCCGAATTA GTGATCATCA AGCCTAAGGG AAAACCCCTT 481 CCATTCTCCT TTGACATACT GTCAACAGCC TTTCAATATG GAAACAGATG CTTCACAAAG 541 TACCCTGCCG ACATGCCTGA CTATTTCAAG CAAGCATTCC CAGATGGAAT GTCATATGAA 601 AGGTCATTTC TATTTGAGGA TGGAGGAGTT GCTACAGCCA GCTGGAGCAT TCGTCTCGAA 661 GGAAATTGCT TCATCCACAA TTCCATCTAT CATGGCGTAA ACTTTCCCGC TGATGGACCC 721 GTAATGAAGA AGCAGACAAT TGGCTGGGAT AAGTCCTTCG AAAAAATGAG TGTGGCTAAA 781 GAGGTGCTAA GANGTGATGT GACTCAGTTT CTTCTGCTCG AANGANGTGG TTACCAGANA 841 TGCCGGTTTC ACTCCACTTA CNAAACGGAN AANCCAGTCG CAATGCCCCC CNNNNCATGN 901 CNNANAACAT CAAANNNNGA GGANCGANNN TGGNNNAACN GNNAANNNNT TCNAGGNCAA 961 NCNGGNANNA NNNTGNNNAN GNNNNNNNNT NNNNCNNTNN NNNNNTNNAN NNANNNNNNN 1021 NNN // |
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Primer: pUC-M13_-100 Region: MCS, insert 3' Sequence file: RDB18265_B0H4b.seq
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>D07039C7_B0H4_1_pUC-M13_-100_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNANGCN NNGNNTTTCC CNNTCNCGAC GTTGTAAAAC 61 GACGGCCAGT GCCNAGCTTG CATGCCTGCA GTTATTTAAC CTTCAAAGGG TTAACATGAG 121 CCTCAGCATG TTCTTCCAGC TTGACCTTGA AGCCTTTTGC AGTTTGGCCA AGGTCGGTCC 181 TCACAATTTG ATGTTCTACG ACATGACTCG GGGGCATTGC GACTGGCTTC TCCGTTTTGT 241 AAGTGGAGTG AAACCGGCAT CTCTGGTAAC CACCTCCTTC GAGCAGAAGA AACTGAGTCA 301 CATCACCTCT TAGCACCTCT TTAGCCACAC TCATTTTTTC GAAGGACTTA TCCCAGCCAA 361 TTGTCTGCTT CTTCATTACG GGTCCATCAG CGGGAAAGTT TACGCCATGA TAGATGGAAT 421 TGTGGATGAA GCAATTTCCT TCGAGACGAA TGCTCCAGCT GGCTGTAGCA ACTCCTCCAT 481 CCTCAAATAG AAATGACCTT TCATATGACA TTCCATCTGG GAATGCTTGC TTGAAATAGT 541 CAGGCATGTC GGCAGGGTAC TTTGTGAAGC ATCTGTTTCC ATATTGAAAG GCTGTTGACA 601 GTATGTCAAA GGAGAATGGA AGGGGTTTTC CCTTANGCTT GATGATCACT AATTCGGACA 661 TCTGTTTCCC TTCGTAANGG TTTCNAGTTC CTACACCTTC GATCGTGAAC TCATGGCCAT 721 TGACACTGCC TTCCATACGG TATTTCGTCC GCATTTCTTG TGCGATGCCT TGCTTTGAAT 781 AAGACACCAT GGTGGGGATC CCCGGGNACC GANCTCGAAT TCNTAATCAT GGTCATANCT 841 GNTTCCTGTG TGAAANTGNT ATCCGCTCAC NATTCCNCAC NACATACGAN CCNGAANCAT 901 NAAGTGNNAA GCCTGGGGNG NCNNATGANT GANCTNANNC ACANNNNNTG NNNNNNNCNC 961 ACNGNCNNNN TNNCNNNNNG NAAANNNNNN NNNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. |
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2020.12.10
GNP_filter3_RDBDEP_html_201104.pl