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CoralHue(TM) Midoriishi-Cyan 1 (pMiCy1-S1)

CoralHue™ Midoriishi-Cyan 1 (pMiCy1-S1)

Catalog number RDB18265
Resource name CoralHue(TM) Midoriishi-Cyan 1 (pMiCy1-S1)
Alternative name AM-V0061M
Clone info. ・For subcloning.
・Midoriishi-Cyan1 is Cyan fluorescent protein that rapidly mature to form fluorescent dimer.
・Ex/Em (nm): 472/495
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Acropara sp. cyan fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18265 CoralHue(TM) Midoriishi-Cyan 1 (pMiCy1-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Map RDB18265_map.png

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18265_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18265_B0H4a.seq check
>D07039C7_B0H4_1_lacIpro-B_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN ANCGNNTCAG TGANCGNNGA NCGGAAGANC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
  121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
  181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG
  301 ATCCCCACCA TGGTGTCTTA TTCAAAGCAA GGCATCGCAC AAGAAATGCG GACGAAATAC
  361 CGTATGGAAG GCAGTGTCAA TGGCCATGAG TTCACGATCG AAGGTGTAGG AACTGGAAAC
  421 CCTTACGAAG GGAAACAGAT GTCCGAATTA GTGATCATCA AGCCTAAGGG AAAACCCCTT
  481 CCATTCTCCT TTGACATACT GTCAACAGCC TTTCAATATG GAAACAGATG CTTCACAAAG
  541 TACCCTGCCG ACATGCCTGA CTATTTCAAG CAAGCATTCC CAGATGGAAT GTCATATGAA
  601 AGGTCATTTC TATTTGAGGA TGGAGGAGTT GCTACAGCCA GCTGGAGCAT TCGTCTCGAA
  661 GGAAATTGCT TCATCCACAA TTCCATCTAT CATGGCGTAA ACTTTCCCGC TGATGGACCC
  721 GTAATGAAGA AGCAGACAAT TGGCTGGGAT AAGTCCTTCG AAAAAATGAG TGTGGCTAAA
  781 GAGGTGCTAA GANGTGATGT GACTCAGTTT CTTCTGCTCG AANGANGTGG TTACCAGANA
  841 TGCCGGTTTC ACTCCACTTA CNAAACGGAN AANCCAGTCG CAATGCCCCC CNNNNCATGN
  901 CNNANAACAT CAAANNNNGA GGANCGANNN TGGNNNAACN GNNAANNNNT TCNAGGNCAA
  961 NCNGGNANNA NNNTGNNNAN GNNNNNNNNT NNNNCNNTNN NNNNNTNNAN NNANNNNNNN
1021 NNN
//
Primer: pUC-M13_-100
Region: MCS, insert 3'
Sequence file: RDB18265_B0H4b.seq check
>D07039C7_B0H4_1_pUC-M13_-100_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANGCN NNGNNTTTCC CNNTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GCCNAGCTTG CATGCCTGCA GTTATTTAAC CTTCAAAGGG TTAACATGAG
  121 CCTCAGCATG TTCTTCCAGC TTGACCTTGA AGCCTTTTGC AGTTTGGCCA AGGTCGGTCC
  181 TCACAATTTG ATGTTCTACG ACATGACTCG GGGGCATTGC GACTGGCTTC TCCGTTTTGT
  241 AAGTGGAGTG AAACCGGCAT CTCTGGTAAC CACCTCCTTC GAGCAGAAGA AACTGAGTCA
  301 CATCACCTCT TAGCACCTCT TTAGCCACAC TCATTTTTTC GAAGGACTTA TCCCAGCCAA
  361 TTGTCTGCTT CTTCATTACG GGTCCATCAG CGGGAAAGTT TACGCCATGA TAGATGGAAT
  421 TGTGGATGAA GCAATTTCCT TCGAGACGAA TGCTCCAGCT GGCTGTAGCA ACTCCTCCAT
  481 CCTCAAATAG AAATGACCTT TCATATGACA TTCCATCTGG GAATGCTTGC TTGAAATAGT
  541 CAGGCATGTC GGCAGGGTAC TTTGTGAAGC ATCTGTTTCC ATATTGAAAG GCTGTTGACA
  601 GTATGTCAAA GGAGAATGGA AGGGGTTTTC CCTTANGCTT GATGATCACT AATTCGGACA
  661 TCTGTTTCCC TTCGTAANGG TTTCNAGTTC CTACACCTTC GATCGTGAAC TCATGGCCAT
  721 TGACACTGCC TTCCATACGG TATTTCGTCC GCATTTCTTG TGCGATGCCT TGCTTTGAAT
  781 AAGACACCAT GGTGGGGATC CCCGGGNACC GANCTCGAAT TCNTAATCAT GGTCATANCT
  841 GNTTCCTGTG TGAAANTGNT ATCCGCTCAC NATTCCNCAC NACATACGAN CCNGAANCAT
  901 NAAGTGNNAA GCCTGGGGNG NCNNATGANT GANCTNANNC ACANNNNNTG NNNNNNNCNC
961 ACNGNCNNNN TNNCNNNNNG NAAANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2020.12.10

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