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CoralHue(TM) humanized monomeric Kusabira-Orange 1 (phmKO1-S1)

CoralHue™ humanized monomeric Kusabira-Orange 1 (phmKO1-S1)

Catalog number RDB18261
Resource name CoralHue(TM) humanized monomeric Kusabira-Orange 1 (phmKO1-S1)
Alternative name AM-V0054M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Kusabira-Orange1 is Orange fluorescent protein
・Ex/Em(nm): 548/561
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18261 CoralHue(TM) humanized monomeric Kusabira-Orange 1 (phmKO1-S1) DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB18261zzk01.seq provided by the depositor
Remarks, protocol and/or map (png) RDB18261_map.png

Original reference

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Fluorescent protein resources (English text)
Featured content Fluorescent protein resources (Japanese text)
Featured content CoralHue™ Fluorescent Protein (Japanese text)
Featured content CoralHue™ Fluorescent Protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18261_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F
Region: MCS, insert 5'
Sequence file: RDB18261_B0H4a.seq check
>D07039C3_B0H4_3_lacIpro-B_F_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANCNNNNTC NGTGANCGAG GANNCGGANA NCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT
  121 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG
  181 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA
  241 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAGCT CGGTACCCGG
  301 GGATCCCCAC CATGGTGAGC GTGATCAAGC CCGAGATGAA GATGAGGTAC TACATGGACG
  361 GCTCCGTCAA TGGGCATGAG TTCACAATCG AGGGTGAGGG CACAGGCAGA CCTTACGAGG
  421 GACATCAGGA GATGACACTG CGCGTCACAA TGGCCAAGGG CGGGCCAATG CCTTTCGCCT
  481 TCGACCTGGT GTCCCACGTG TTCTGTTACG GCCACAGACC TTTTACTAAA TATCCAGAAG
  541 AGATCCCAGA CTATTTCAAG CAGGCCTTTC CTGAGGGCCT GTCCTGGGAG AGGTCCCTGG
  601 AGTTCGAGGA CGGCGGCTCC GCCTCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACACCT
  661 TCTACCACAA GTCCAAGTTC ACCGGCGTGA ACTTCCCCGC CGACGGCCCC ATCATGCAGA
  721 ACCAGAGCGT GGACTGGGAG CCCTCCACCG ANAAGATCAC CGCCNGCGAC GGCGTGCTGA
  781 AGGGCGACGT GACCATGTAC CTGAAGCTGG ANGGCGGCGG CAACCACAAG TGCCAGTTCA
  841 AGACCACCTA CAAGGNCGCC NANAAGATNC TGNNNANGNC CGGCNNCCAC TACATCANCC
901 NCANGNTGGT GANGAAGANC GANGNNNNCA TCACCNANCN NNNGGANGNN NNNNNNNN
//
Primer: MBL_S1-rev1
Region: MCS, insert 3'
Sequence file: RDB18261_B0H4b.seq check
>D07039C3_B0H4_3_MBL_S1-rev1_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNGNNCGG NGCGGGCCTC TTCGCTATTA CGCCNGCTGG
   61 CGAAAGGGGG NNGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC
  121 GACGTTGTAA AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGTTAGGA GTGGGCCACG
  181 GCGTCCTCCA CCAGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCTGATG
  241 TAGTGGCTGC CGGGCATCTT CAGGATCTTC TTGGCGGCCT TGTAGGTGGT CTTGAACTGG
  301 CACTTGTGGT TGCCGCCGCC CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG
  361 CCGTCGCTGG CGGTGATCTT CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG
  421 ATGGGGCCGT CGGCGGGGAA GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG
  481 CCCCTCAGGC TGATGTGGGC GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC
  541 CTCTCCCAGG ACAGGCCCTC AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGA
  601 TATTTAGTAA AAGGTCTGTG GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA
  661 GGCATTGGCC CGCCCTTGGC CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA
  721 GGTCTGCCTG TGCCCTCACC CTCGATTGTG AACTCATGCC CATTGACGGA NCCGTCCATG
  781 TAGTACCTCA TCTTCATCTC GGGCTTGATC ACGCTCACCA TGGNGGGGGN TCCCNNGGGT
  841 ANCGANCTCG AATTCNTAAT CNTGGTCATA NCTNNTTCCT GTGNGAAATT GTTATCCGCT
  901 CNCAATTCCN CACAACATAC NANCCNGNAN NATAAAGNGT AAAGCCNGGG GGNNNCNNAN
961 GANNNNNCNN ACNCANNNNN NNTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

2021.08.13

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