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CoralHue™ humanized monomeric Kusabira-Orange 1 (phmKO1-S1)
| Catalog number | RDB18261 |
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| Resource name | CoralHue(TM) humanized monomeric Kusabira-Orange 1 (phmKO1-S1) |
| Alternative name | AM-V0054M |
| Clone info. | ・For subcloning. ・The codons are humanized. ・Monomeric Kusabira-Orange1 is Orange fluorescent protein ・Ex/Em(nm): 548/561 |
| Vector backbone | S1 (plasmid) |
| Size of vector backbone | 2.7 kb |
| Selectable markers | Ampicillin |
| Gene/insert name | Fungia concinna orange fluorescent protein cDNA |
| Depositor|Developer | MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA. |
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| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB18261 | CoralHue(TM) humanized monomeric Kusabira-Orange 1 (phmKO1-S1) | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Nucleotide sequence provided by the depositor RDB18261zzk01.seq |
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| Electronic file | Map RDB18261_map.png
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Original reference
| original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Fluorescent protein resources (English text) |
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| Featured content | Fluorescent protein resources (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (Japanese text) |
| Featured content | CoralHue™ Fluorescent Protein (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB18261_B0H4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: lacIpro-B_F Region: MCS, insert 5' Sequence file: RDB18261_B0H4a.seq
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>D07039C3_B0H4_3_lacIpro-B_F_D05_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NANCNNNNTC NGTGANCGAG GANNCGGANA NCGCCCAATA 61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT 121 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG 181 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 241 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG AATTCGAGCT CGGTACCCGG 301 GGATCCCCAC CATGGTGAGC GTGATCAAGC CCGAGATGAA GATGAGGTAC TACATGGACG 361 GCTCCGTCAA TGGGCATGAG TTCACAATCG AGGGTGAGGG CACAGGCAGA CCTTACGAGG 421 GACATCAGGA GATGACACTG CGCGTCACAA TGGCCAAGGG CGGGCCAATG CCTTTCGCCT 481 TCGACCTGGT GTCCCACGTG TTCTGTTACG GCCACAGACC TTTTACTAAA TATCCAGAAG 541 AGATCCCAGA CTATTTCAAG CAGGCCTTTC CTGAGGGCCT GTCCTGGGAG AGGTCCCTGG 601 AGTTCGAGGA CGGCGGCTCC GCCTCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACACCT 661 TCTACCACAA GTCCAAGTTC ACCGGCGTGA ACTTCCCCGC CGACGGCCCC ATCATGCAGA 721 ACCAGAGCGT GGACTGGGAG CCCTCCACCG ANAAGATCAC CGCCNGCGAC GGCGTGCTGA 781 AGGGCGACGT GACCATGTAC CTGAAGCTGG ANGGCGGCGG CAACCACAAG TGCCAGTTCA 841 AGACCACCTA CAAGGNCGCC NANAAGATNC TGNNNANGNC CGGCNNCCAC TACATCANCC 901 NCANGNTGGT GANGAAGANC GANGNNNNCA TCACCNANCN NNNGGANGNN NNNNNNNN // |
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Primer: MBL_S1-rev1 Region: MCS, insert 3' Sequence file: RDB18261_B0H4b.seq
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>D07039C3_B0H4_3_MBL_S1-rev1_A09_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNGNNCGG NGCGGGCCTC TTCGCTATTA CGCCNGCTGG 61 CGAAAGGGGG NNGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC 121 GACGTTGTAA AACGACGGCC AGTGCCAAGC TTGCATGCCT GCAGTTAGGA GTGGGCCACG 181 GCGTCCTCCA CCAGCTCGGT GATGTTGCCC TCGGTCTTCC TCACCAGCCT GTGGCTGATG 241 TAGTGGCTGC CGGGCATCTT CAGGATCTTC TTGGCGGCCT TGTAGGTGGT CTTGAACTGG 301 CACTTGTGGT TGCCGCCGCC CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG 361 CCGTCGCTGG CGGTGATCTT CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG 421 ATGGGGCCGT CGGCGGGGAA GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG 481 CCCCTCAGGC TGATGTGGGC GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC 541 CTCTCCCAGG ACAGGCCCTC AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGA 601 TATTTAGTAA AAGGTCTGTG GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA 661 GGCATTGGCC CGCCCTTGGC CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA 721 GGTCTGCCTG TGCCCTCACC CTCGATTGTG AACTCATGCC CATTGACGGA NCCGTCCATG 781 TAGTACCTCA TCTTCATCTC GGGCTTGATC ACGCTCACCA TGGNGGGGGN TCCCNNGGGT 841 ANCGANCTCG AATTCNTAAT CNTGGTCATA NCTNNTTCCT GTGNGAAATT GTTATCCGCT 901 CNCAATTCCN CACAACATAC NANCCNGNAN NATAAAGNGT AAAGCCNGGG GGNNNCNNAN 961 GANNNNNCNN ACNCANNNNN NNTNNNNNNN NNN // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. |
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2020.12.28
GNP_filter3_RDBDEP_html_201222.pl