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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1)

CoralHue™ monomeric Kusabira-Orange 1 (pmKO1-S1)

Catalog number RDB18258
Resource name CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1)
Alternative name AM-V0051M
Clone info. ・For subcloning.
・Monomeric Kusabira-Orange1 is Orange fluorescent protein.
・Ex/Em(nm): 548/561
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
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External Database
Fungia concinna orange fluorescent protein

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
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            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18258 CoralHue(TM) monomeric Kusabira-Orange 1 (pmKO1-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB18258zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18258_map.png

            Original reference

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18258_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F
            Region: MCS, mKO1 5'
            Sequence file: RDB18258_B0H4a.seq check
            >D07039B9_B0H4_4_pUC-CAPbs_up_F_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ACGCNANNTA ATGTGANNNN NNTCNCTCNT
               61 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
              121 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGAATTCGA GCTCGGTACC
              181 CGGGGATCCC CACCATGGTG AGTGTGATTA AACCAGAGAT GAAGATGAGG TACTACATGG
              241 ACGGCTCCGT CAATGGGCAT GAGTTCACAA TTGAAGGTGA AGGCACAGGC AGACCTTACG
              301 AGGGACATCA AGAGATGACA CTACGCGTCA CAATGGCCAA GGGCGGGCCA ATGCCTTTCG
              361 CGTTTGACTT AGTGTCACAC GTGTTCTGTT ACGGCCACAG ACCTTTTACT AAATATCCAG
              421 AAGAGATACC AGACTATTTC AAACAAGCAT TTCCTGAAGG CCTGTCATGG GAAAGGTCGT
              481 TGGAGTTCGA AGATGGTGGG TCCGCTTCAG TCAGTGCGCA TATAAGCCTT AGAGGAAACA
              541 CCTTCTACCA CAAATCCAAA TTTACTGGGG TTAACTTTCC TGCCGATGGT CCTATCATGC
              601 AAAACCAAAG TGTTGATTGG GAGCCATCAA CCGAGAAAAT TACTGCCAGC GACGGAGTTC
              661 TGAAGGGTGA TGTTACGATG TACCTAAAAC TTGAAGGAGG CGGCAATCAC AAATGCCAAT
              721 TCAAGACTAC TTACAAGGCG GCAAAAAAGA TTCTTAAAAT GCCNGGAAGC CATTACATCA
              781 NCNNTCGCCT CGTCAGGAAA ACCGAANGCA ACATTACTGA NCTGGTAGAA GANGCANNAN
              841 CTCATTCCTA ACTGCANGCA TGCAAGCTNG GCACTGNNCG TCGTTTTANN ACGTCGNGNC
              901 TGGGAAAANC CNNNNNTNNN CNNACTTNNA NCGCCNNGCN NCNNATNCNN NNNTNNNNNA
            961 GCTGGNNNNN NNNCNNANNN GNCCNNNNNN ATCNNNNNNN CNNNNNNNNN N
            //
            Primer: pUC-M13_-100
            Region: MCS, mKO1
            Sequence file: RDB18258_B0H4b.seq check
            >D07039B9_B0H4_4_pUC-M13_-100_G01_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNANGCNNN GGTTTTCCNN GTCNCGACGT TGTAAAACGA
               61 CGGCCAGTGC CAAGCTTGNA TGCCTGCAGT TAGGAATGAG CTACTGCATC TTCTACCAGC
              121 TCAGTAATGT TGCCTTCGGT TTTCCTGACG AGGCGATGGC TGATGTAATG GCTTCCTGGC
              181 ATTTTAAGAA TCTTTTTTGC CGCCTTGTAA GTAGTCTTGA ATTGGCATTT GTGATTGCCG
              241 CCTCCTTCAA GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA
              301 ATTTTCTCGG TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA
              361 GGAAAGTTAA CCCCAGTAAA TTTGGATTTG TGGTAGAAGG TGTTTCCTCT AAGGCTTATA
              421 TGCGCACTGA CTGAAGCGGA CCCACCATCT TCGAACTCCA ACGACCTTTC CCATGACAGG
              481 CCTTCAGGAA ATGCTTGTTT GAAATAGTCT GGTATCTCTT CTGGATATTT AGTAAAAGGT
              541 CTGTGGCCGT AACAGAACAC GTGTGACACT AAGTCAAACG CGAAAGGCAT TGGCCCGCCC
              601 TTGGCCATTG TGACGCGTAG TGTCATCTCT TGATGTCCCT CGTAAGGTCT GCCTGTGCCT
              661 TCACCTTCAA TTGTGAACTC ATGCCCATTG ACGGAGCCGT CCATGTAGTA CCTCATCTTC
              721 ATCTCTGGTT TAATCACACT CACCATGGTG GGGNNCCCCG GGTACCGAGC TCGAATTCGT
              781 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
              841 TACGAGCCGG NNNCATAAAG TGTAAAGCCT GGGGTGNCTA NTGANTGANC TAACTCACAT
              901 TAATTGCGTT GCGCTCACTG CCCGCTTNCC AGTCNGGAAA NNTGNCNNGN NNNCTGCNNN
            961 AANNNNTCNG NCNAACGCNN NGGGNNNANG NNGNNTNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

            2022.04.05

            GNP_filter3_RDBDEP_html_220305.pl