Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1) (#RDB18254)

CoralHue™ humanized Kusabira-Orange 1 (phKO1-S1)

Alternative name AM-V0044M
Clone info. ・For subcloning.
・The codons are humanized.
・Kusabira-Orange1 is Orange fluorescent protein that rapidly mature to form fluorescent dimer.
・Ex/Em(nm): 548/561
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18254zzk02.seq provided by the depositor
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18254 CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18254).

Reference section:

Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18254_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18254_B0H4a.seq check
>D07039B5_B0H4_16_lacIpro-B_F_A06_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN NCGNNTCNGT GAGCGAGGAA NCGGANANCG CCCAATACGC
   61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
  121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
  181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA
  301 TCCCCACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT GAAGTACTTC ATGGACGGCA
  361 GCGTGAACGG CCACGAGTTC ACCGTGGAGG GCGAGGGCAC CGGCAAGCCC TACGAGGGCC
  421 ACCAGGAGAT GACCCTGAGG GTGACAATGG CCAAGGGCGG CCCCATGCCC TTCAGCTTCG
  481 ACCTGGTGAG CCACACCTTC TGCTACGGCC ACAGGCCCTT CACCAAGTAC CCCGAGGAGA
  541 TCCCCGACTA CTTCAAGCAG GCCTTCCCCG AGGGCCTGAG CTGGGAGAGG AGCCTCCAGT
  601 TCGAGGACGG CGGCTTCGCC GCCGTGAGCG CCCACATCAG CCTGAGGGGC AACTGCTTCG
  661 AGCACAAGAG CAAGTTCGTG GGCGTGAACT TCCCCGCCGA CGGCCCCGTG ATGCAGAACC
  721 AGAGCAGCGA CTGGGAGCCC AGCACCGANA AGATCACCAC CTGCGACGGC GTGCTGAAGG
  781 GCGACGTGAC CATGTACCTG AAGCTGGNCG GCGGCGGCAA CCACAAGTGC CAGTTCNAGA
  841 NCACCTACNA NGCCGCCNAG AANANNCTNA AGATGCCCCA NANCCACTTC ATCNGNCNNN
  901 NNCNNGNGAG GAAGANCGAN GGNNNCATCN NNGANNNNNN NGNANNNNGN CNNNNNNNNN
961 N
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert
Sequence file: RDB18254_B0H4b.seq check
>D07039B5_B0H4_16_pUC-M13_-100_B06_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNANGCC NNNGTTTTCC CNGTCNCGAC GTTGTAAAAC
   61 GACGGCCAGT GCCAAGCTTG CATGCCTGCA GTCAGCAGTG GGCCACGGCG TCCTCCACCA
  121 GCTCGGTGAT GTTGCCCTCG GTCTTCCTCA CCAGCCTGTG GCCGATGAAG TGGCTCTGGG
  181 GCATCTTCAG GATCTTCTTG GCGGCCTTGT AGGTGGTCTT GAACTGGCAC TTGTGGTTGC
  241 CGCCGCCGGC CAGCTTCAGG TACATGGTCA CGTCGCCCTT CAGCACGCCG TCGCAGGTGG
  301 TGATCTTCTC GGTGCTGGGC TCCCAGTCGC TGCTCTGGTT CTGCATCACG GGGCCGTCGG
  361 CGGGGAAGTT CACGCCCACG AACTTGCTCT TGTGCTCGAA GCAGTTGCCC CTCAGGCTGA
  421 TGTGGGCGCT CACGGCGGCG AAGCCGCCGT CCTCGAACTG GAGGCTCCTC TCCCAGCTCA
  481 GGCCCTCGGG GAAGGCCTGC TTGAAGTAGT CGGGGATCTC CTCGGGGTAC TTGGTGAAGG
  541 GCCTGTGGCC GTAGCAGAAG GTGTGGCTCA CCAGGTCGAA GCTGAAGGGC ATGGGGCCGC
  601 CCTTGGCCAT TGTCACCCTC AGGGTCATCT CCTGGTGGCC CTCGTAGGGC TTGCCGGTGC
  661 CCTCGCCCTC CACGGTGAAC TCGTGGCCGT TCACGCTGCC GTCCATGAAG TACTTCATCT
  721 TCATCTCGGG CTTGATCACG CTCACCATGG TGGGGATNCC CGGGTACCGA NCTCGAATTC
  781 GTAATCATGG TCATAGCTGT TTCCTGTNTG AAATTGTTAT CCGCTCACAA TTCCNCACAA
  841 CATACGANCC NGAAGCATAA AGNGTAAAGC CTGGGGNGNC TNANGANNGA GCNNNNTCAC
  901 ATTANNTGCG TTNCGNNNAC NNNNNNNNTT TCCNNTCNGN AAANCTGNCN NGNNNNNNTN
961 NNTNANNNNN NNNGNCCNNN NNCNNNGGGN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984. [link to RRC of NBRP]