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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1)

CoralHue™ humanized Kusabira-Orange 1 (phKO1-S1)

Catalog number RDB18254
Resource name CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1)
Alternative name AM-V0044M
Clone info. ・For subcloning.
・The codons are humanized.
・Kusabira-Orange1 is Orange fluorescent protein that rapidly mature to form fluorescent dimer.
・Ex/Em(nm): 548/561
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Fungia concinna orange fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
Other clones in our bank
External Database
Fungia concinna orange fluorescent protein
            Reference sequence
              
             
            Sequence (full) RDB18254zzk02.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18254_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312, 2004)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18254 CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CoralHue(TM) humanized Kusabira-Orange 1 (phKO1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18254).

            Reference section:

            Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content CoralHue™ Fluorescent Protein (English text)
            Featured content CoralHue™ Fluorescent Protein (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18254_B0H4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F (Pr0724)
            Region: MCS, insert 5'
            Sequence file: RDB18254_B0H4a.seq check
            >D07039B5_B0H4_16_lacIpro-B_F_A06_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNGNN NCGNNTCNGT GAGCGAGGAA NCGGANANCG CCCAATACGC
               61 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
              121 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
              181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
              241 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGAAT TCGAGCTCGG TACCCGGGGA
              301 TCCCCACCAT GGTGAGCGTG ATCAAGCCCG AGATGAAGAT GAAGTACTTC ATGGACGGCA
              361 GCGTGAACGG CCACGAGTTC ACCGTGGAGG GCGAGGGCAC CGGCAAGCCC TACGAGGGCC
              421 ACCAGGAGAT GACCCTGAGG GTGACAATGG CCAAGGGCGG CCCCATGCCC TTCAGCTTCG
              481 ACCTGGTGAG CCACACCTTC TGCTACGGCC ACAGGCCCTT CACCAAGTAC CCCGAGGAGA
              541 TCCCCGACTA CTTCAAGCAG GCCTTCCCCG AGGGCCTGAG CTGGGAGAGG AGCCTCCAGT
              601 TCGAGGACGG CGGCTTCGCC GCCGTGAGCG CCCACATCAG CCTGAGGGGC AACTGCTTCG
              661 AGCACAAGAG CAAGTTCGTG GGCGTGAACT TCCCCGCCGA CGGCCCCGTG ATGCAGAACC
              721 AGAGCAGCGA CTGGGAGCCC AGCACCGANA AGATCACCAC CTGCGACGGC GTGCTGAAGG
              781 GCGACGTGAC CATGTACCTG AAGCTGGNCG GCGGCGGCAA CCACAAGTGC CAGTTCNAGA
              841 NCACCTACNA NGCCGCCNAG AANANNCTNA AGATGCCCCA NANCCACTTC ATCNGNCNNN
              901 NNCNNGNGAG GAAGANCGAN GGNNNCATCN NNGANNNNNN NGNANNNNGN CNNNNNNNNN
            961 N
            //
            Primer: pUC-M13_-100 (Pr0310)
            Region: MCS, insert
            Sequence file: RDB18254_B0H4b.seq check
            >D07039B5_B0H4_16_pUC-M13_-100_B06_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGGNANGCC NNNGTTTTCC CNGTCNCGAC GTTGTAAAAC
               61 GACGGCCAGT GCCAAGCTTG CATGCCTGCA GTCAGCAGTG GGCCACGGCG TCCTCCACCA
              121 GCTCGGTGAT GTTGCCCTCG GTCTTCCTCA CCAGCCTGTG GCCGATGAAG TGGCTCTGGG
              181 GCATCTTCAG GATCTTCTTG GCGGCCTTGT AGGTGGTCTT GAACTGGCAC TTGTGGTTGC
              241 CGCCGCCGGC CAGCTTCAGG TACATGGTCA CGTCGCCCTT CAGCACGCCG TCGCAGGTGG
              301 TGATCTTCTC GGTGCTGGGC TCCCAGTCGC TGCTCTGGTT CTGCATCACG GGGCCGTCGG
              361 CGGGGAAGTT CACGCCCACG AACTTGCTCT TGTGCTCGAA GCAGTTGCCC CTCAGGCTGA
              421 TGTGGGCGCT CACGGCGGCG AAGCCGCCGT CCTCGAACTG GAGGCTCCTC TCCCAGCTCA
              481 GGCCCTCGGG GAAGGCCTGC TTGAAGTAGT CGGGGATCTC CTCGGGGTAC TTGGTGAAGG
              541 GCCTGTGGCC GTAGCAGAAG GTGTGGCTCA CCAGGTCGAA GCTGAAGGGC ATGGGGCCGC
              601 CCTTGGCCAT TGTCACCCTC AGGGTCATCT CCTGGTGGCC CTCGTAGGGC TTGCCGGTGC
              661 CCTCGCCCTC CACGGTGAAC TCGTGGCCGT TCACGCTGCC GTCCATGAAG TACTTCATCT
              721 TCATCTCGGG CTTGATCACG CTCACCATGG TGGGGATNCC CGGGTACCGA NCTCGAATTC
              781 GTAATCATGG TCATAGCTGT TTCCTGTNTG AAATTGTTAT CCGCTCACAA TTCCNCACAA
              841 CATACGANCC NGAAGCATAA AGNGTAAAGC CTGGGGNGNC TNANGANNGA GCNNNNTCAC
              901 ATTANNTGCG TTNCGNNNAC NNNNNNNNTT TCCNNTCNGN AAANCTGNCN NGNNNNNNTN
            961 NNTNANNNNN NNNGNCCNNN NNCNNNGGGN NNNNGNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Karasawa, S., Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem. J. 381 (Pt 1): 307-312 (2004). PMID 15065984.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl