RDB18234 - pCI-neo-mPCFT

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pCI-neo-mPCFT (#RDB18234)

Expression vector of mouse PCFT.

Clone info.	Expression vector of mouse proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment	A reference nucleotide sequence for Mouse PCFT is NM_026740.2.
Vector backbone	pCl-neo (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name	mouse Slc46a1 cDNA
Depositor\|Developer	Yuasa, Hiroaki \|

Sequence (full)	RDB18234zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18234	pCI-neo-mPCFT	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-mPCFT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18234).

Reference section:

Yamashiro, T., Yasujima, T., Ohta, K., Inoue, K., Yuasa, H., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18234_B0Fup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: chimeric intron 3-prime (Pr0444) Region: insert 5' Sequence file: RDB18234_B0Fua.seq
>D07213A5_B0Fu_2_chimeric intron3-prime_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNTNNN NGCTCTTNNG CTAGAGTACT TAATACGACT CACTATAGGC 61 TAGCCTCGNG AATTCGAGCA TGGAGGGGCG CGTGAGCTCC GTGGGCTCGC CCCACAGTTT 121 CCTCAACGCT CCCGTGCTTT TCCGCGGCCC CGTGGAGCCC CTAGTCTTCC TGGCCAACTT 181 TGCCCTGGTT CTGCAGGGAC CGCTCACCAC ACAGTACCTT TGGCACCGGT TCAGCACGGA 241 GCTCGGCTAC AATGGCACCC GCCACCGGGA GAACTGTGGG AACCAAAGCG CGGATCCCCT 301 CATGAAGGAA GTGGAGACCC TGACATCTCA CTGGACCCTC TACATGAACG TGGGAGGCTT 361 CCTGGTGGGA CTCTTCTGGT CCACCCTGCT GGGAGCCTGG AGTGACCGCG TGGGTCGCCG 421 CCCATTGCTG GTGCTGGCCT CTCTTGGTCT GCTGCTCCAG GCCGTGGTGT CCATCTTTGT 481 GGTGCAGCTA GAACTGCACG TCGGGTTCTT CGTGCTTGGC CGAGCTCTTT GTGCCCTTCT 541 GGGAGACTTC AACGGCCTCC TTGCTGCTAG CTTTGCCTCT GTGGCAGATG TTAGCTCTAA 601 CCACAGTCGC ACCTTCCGGA TGGCTCTGCT GGAANCATGC ATTGGTGTGG CCGGGANCCT 661 GGCAAGCCTT CTCGGGGGTC ACTGGCTCCG GGCCCAGGGT TATGCCAACC CCTTCTGGCT 721 GGCTTTGGCC CTGCTGATTG TCATGGCTCT ATACGCAGCA TTTTGTTTTG GTGANACNGT 781 GAANGANCCN ANNNNNANCA NGNTTTTCNC GCNNCNCCNT CATCNATNCN TTNCCCNGNT 841 ATATGNGGTT NCNNCCNCAN AAAANTCCAG GANGCNNNNA NNCCNNNANT NNTNNGNCNN 901 NANNNNNNNN GGNTNNNNNN NNNN //
Primer: pAxCALNLF1 (Pr0166) Region: insert 3' Sequence file: RDB18234_B0Fub.seq
>D07213A5_B0Fu_2_pAxCALNLF1_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTGNCTGCTC NNNNNANTAA CCCTCACTAA AGGGAAGCGG 61 CCGCCCGGGN CNNNNNNNNA AGATCAGGGG CTCTGAGGAA ACTGCTGGAA CTCCGGGTGT 121 GGATTAACCT TTTCCAGTAC CCCAATCAGA ATGGCTGGAA TGAAGAGGAG GCCAGCTCCC 181 AGGAGGAAGG GGAAGCCCTT CATGAAGTTC AGAGTGGCCG GGTAGATGGA GTTGAAGATG 241 CCGGAGGCCA TCAACATGGC CAAGCTATTC ACACAGGCCA CAGCAGAGAA CAGAGCACCC 301 TGCTCCGACT CGCTCACCAG CTTGGAAAGC TTGGCCCGGA TGACAGGCGT CGTGACCAAT 361 GACAGGAAGA GCAATCCGTA CCCTGTGAAC ATGAGGGGTG TAATGGTAGC AAACGCAAAG 421 ACCACCATTC CCAGGATGTT GAAAGCCAGG CCAATCTCAG CCACCCAGGT GTCAGCCAGG 481 CAGAATTGCA GAAGCCTTAG GCCCAGCAGG CTGGTGAGGT AGGGTAGGTG CTGGGCTGCT 541 GAGCCATAGC CAATCAGCTT GGAGTCCCAG CAGAGGGGTG CGCTCAGCTC ATAGAGTGTC 601 AGGATATCTT GAGCCCCAAA ATGCACAGTA ACCACCACGA ATATGGCCAA AGAGTACAGG 661 GCTAGATGCA TCCTGNANTT TTCTGGGGCT GGAACCACAT ATAGCCGGGC AATGGATCGA 721 TGATGGCGGA NCGTGAAAAG CCTGGTGGNN TTTGGCTCCT TCNCCGTCTC ACCNAAACAA 781 AATGCTGCGT ATANNNCCAT GACAATCAGC NNGNCCAAAG CCAGCCAGAA NGGGTTGNNA 841 TNNCCCNNGG CCCNGNNNCA NNNNNCCCNN ANAANNNNTN GCCNNGGNNC CCNGNCCNCA 901 NNNNNNCNNG CNTTCCNNNA NNNNNNNTNN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: NeoR_KanR Sequence file: RDB18234_B0Fuc.seq
>D07213A5_B0Fu_2_SV40pro_ori_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT NNNCTATTCC NGAAGTAGTG AGGAGGCTTT 61 TTTNNNNNNN NNNGNTTTTG CAAAAAGCTT GATTCTTCTG ACACAACANT CTCGAACTTA 121 AGGCTAGAGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 661 CGCGCATGCC CGACGGCGAN GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 721 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACNG TGGCCGGCTG GGNGTNGCGG 781 ANCGCTATCA NGACATANCG NTGGCTACNC GNGATATTGC TGAAGANCTT GGCGGCGAAN 841 GGGCTNANCG CTTCCNCNNG CNTTACNGNA TCGCCGCNNC NNATTCNCAN CGNATCNNCN 901 NNNANCGCCT TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273. [link to RRC of NBRP]

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Other resources in our bank

mouse Slc46a1

External Database
mouse Slc46a1

Reference sequence