Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCI-neo-mPCFT

Expression vector of mouse PCFT.

Catalog number RDB18234
Resource name pCI-neo-mPCFT
Clone info. Expression vector of mouse proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment A reference nucleotide sequence for Mouse PCFT is NM_026740.2.
Vector backbone pCl-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name mouse Slc46a1 cDNA
Depositor|Developer Yuasa, Hiroaki |
Other clones in our bank
External Database
mouse Slc46a1
          Reference sequence
            
           
          Sequence (full) RDB18234zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Yamashiro T. et al. Sci. Rep. 9, 18105, 2019)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18234 pCI-neo-mPCFT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18234_B0Fup1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: chimeric intron 3-prime
          Region: insert 5'
          Sequence file: RDB18234_B0Fua.seq check
          >D07213A5_B0Fu_2_chimeric intron3-prime_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNNN NGCTCTTNNG CTAGAGTACT TAATACGACT CACTATAGGC
             61 TAGCCTCGNG AATTCGAGCA TGGAGGGGCG CGTGAGCTCC GTGGGCTCGC CCCACAGTTT
            121 CCTCAACGCT CCCGTGCTTT TCCGCGGCCC CGTGGAGCCC CTAGTCTTCC TGGCCAACTT
            181 TGCCCTGGTT CTGCAGGGAC CGCTCACCAC ACAGTACCTT TGGCACCGGT TCAGCACGGA
            241 GCTCGGCTAC AATGGCACCC GCCACCGGGA GAACTGTGGG AACCAAAGCG CGGATCCCCT
            301 CATGAAGGAA GTGGAGACCC TGACATCTCA CTGGACCCTC TACATGAACG TGGGAGGCTT
            361 CCTGGTGGGA CTCTTCTGGT CCACCCTGCT GGGAGCCTGG AGTGACCGCG TGGGTCGCCG
            421 CCCATTGCTG GTGCTGGCCT CTCTTGGTCT GCTGCTCCAG GCCGTGGTGT CCATCTTTGT
            481 GGTGCAGCTA GAACTGCACG TCGGGTTCTT CGTGCTTGGC CGAGCTCTTT GTGCCCTTCT
            541 GGGAGACTTC AACGGCCTCC TTGCTGCTAG CTTTGCCTCT GTGGCAGATG TTAGCTCTAA
            601 CCACAGTCGC ACCTTCCGGA TGGCTCTGCT GGAANCATGC ATTGGTGTGG CCGGGANCCT
            661 GGCAAGCCTT CTCGGGGGTC ACTGGCTCCG GGCCCAGGGT TATGCCAACC CCTTCTGGCT
            721 GGCTTTGGCC CTGCTGATTG TCATGGCTCT ATACGCAGCA TTTTGTTTTG GTGANACNGT
            781 GAANGANCCN ANNNNNANCA NGNTTTTCNC GCNNCNCCNT CATCNATNCN TTNCCCNGNT
            841 ATATGNGGTT NCNNCCNCAN AAAANTCCAG GANGCNNNNA NNCCNNNANT NNTNNGNCNN
          901 NANNNNNNNN GGNTNNNNNN NNNN
          //
          Primer: pAxCALNLF1
          Region: insert 3'
          Sequence file: RDB18234_B0Fub.seq check
          >D07213A5_B0Fu_2_pAxCALNLF1_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTGNCTGCTC NNNNNANTAA CCCTCACTAA AGGGAAGCGG
             61 CCGCCCGGGN CNNNNNNNNA AGATCAGGGG CTCTGAGGAA ACTGCTGGAA CTCCGGGTGT
            121 GGATTAACCT TTTCCAGTAC CCCAATCAGA ATGGCTGGAA TGAAGAGGAG GCCAGCTCCC
            181 AGGAGGAAGG GGAAGCCCTT CATGAAGTTC AGAGTGGCCG GGTAGATGGA GTTGAAGATG
            241 CCGGAGGCCA TCAACATGGC CAAGCTATTC ACACAGGCCA CAGCAGAGAA CAGAGCACCC
            301 TGCTCCGACT CGCTCACCAG CTTGGAAAGC TTGGCCCGGA TGACAGGCGT CGTGACCAAT
            361 GACAGGAAGA GCAATCCGTA CCCTGTGAAC ATGAGGGGTG TAATGGTAGC AAACGCAAAG
            421 ACCACCATTC CCAGGATGTT GAAAGCCAGG CCAATCTCAG CCACCCAGGT GTCAGCCAGG
            481 CAGAATTGCA GAAGCCTTAG GCCCAGCAGG CTGGTGAGGT AGGGTAGGTG CTGGGCTGCT
            541 GAGCCATAGC CAATCAGCTT GGAGTCCCAG CAGAGGGGTG CGCTCAGCTC ATAGAGTGTC
            601 AGGATATCTT GAGCCCCAAA ATGCACAGTA ACCACCACGA ATATGGCCAA AGAGTACAGG
            661 GCTAGATGCA TCCTGNANTT TTCTGGGGCT GGAACCACAT ATAGCCGGGC AATGGATCGA
            721 TGATGGCGGA NCGTGAAAAG CCTGGTGGNN TTTGGCTCCT TCNCCGTCTC ACCNAAACAA
            781 AATGCTGCGT ATANNNCCAT GACAATCAGC NNGNCCAAAG CCAGCCAGAA NGGGTTGNNA
            841 TNNCCCNNGG CCCNGNNNCA NNNNNCCCNN ANAANNNNTN GCCNNGGNNC CCNGNCCNCA
          901 NNNNNNCNNG CNTTCCNNNA NNNNNNNTNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: NeoR_KanR
          Sequence file: RDB18234_B0Fuc.seq check
          >D07213A5_B0Fu_2_SV40pro_ori_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT NNNCTATTCC NGAAGTAGTG AGGAGGCTTT
             61 TTTNNNNNNN NNNGNTTTTG CAAAAAGCTT GATTCTTCTG ACACAACANT CTCGAACTTA
            121 AGGCTAGAGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
            181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
            241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
            301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
            361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
            421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
            481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
            541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
            601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
            661 CGCGCATGCC CGACGGCGAN GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
            721 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACNG TGGCCGGCTG GGNGTNGCGG
            781 ANCGCTATCA NGACATANCG NTGGCTACNC GNGATATTGC TGAAGANCTT GGCGGCGAAN
            841 GGGCTNANCG CTTCCNCNNG CNTTACNGNA TCGCCGCNNC NNATTCNCAN CGNATCNNCN
          901 NNNANCGCCT TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Yamashiro, T., Identification of the amino acid residue responsible for the myricetin sensitivity of human proton-coupled folate transporter. Sci. Rep. 9 (1): 18105 (2019). PMID 31792273.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl