RDB18231 - pCI-neo-rPCFT

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pCI-neo-rPCFT (#RDB18231)

Expression vector of rat PCFT.

Clone info.	Expression vector of rat proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment	A reference nucleotide sequence for Rat PCFT is NM_001013969.1.
Vector backbone	pCl-neo (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name	rat Slc46a1 cDNA
Depositor\|Developer	Yuasa, Hiroaki \|

Sequence (full)	RDB18231zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inoue K. et al. Am. J. Physiol. 294, G660-G668, 2008)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18231	pCI-neo-rPCFT	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-rPCFT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18231).

Reference section:

Inoue, K., Nakai, Y., Ueda, S., Kamigaso, S., Ohta, K.Y., Hatakeyama, M., Hayashi, Y., Otagiri, M., Yuasa, H., Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. Am. J. Physiol. Gastrointest. Liver Physiol. 294(3): G660-668 (2008). PMID 18174275. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18231_B0Fup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: chimeric intron 3-prime (Pr0444) Region: T7 pro, insert 5' Sequence file: RDB18231_B0Fua.seq
>D07213A2_B0Fu_2_chimeric intron3-prime_D06_12_ABI24.ab1 1 NNNNNNNNNN NCNNNNNNNN NNNCNGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA 61 TAGGCTAGCC TCGAGAATTC CGGAATTTCC TCGAGGTCGA CAGATCTGAT GTGTCAGCCC 121 CTCGGTGTTC GCGCATGGAG GGGCGCGTGA GCCCCGTGGG CTCGTCCCAC CGTCTCCTCA 181 CCGCCGCCGT GCTGTTCCGC GGCCCCGTGG AGCCCCTCGT TTTCCTAGCC AACTTTGCGC 241 TGGTTCTACA GGGACCGCTC ACCACACAGT ACATTTGGCA CCGCATCAGC ACCGAGCTCG 301 GATACAATGG CACCCGCCAC CGGGAGAACT GTGGCAACCA GAGCGCGGAT CCCGTCCTGA 361 AGGAAGTGGA GACCCTGACA TCCCACTGGA CCCTCTACAT GAACGTGGGA GGCTTCCTGG 421 TGGGACTCTT CTGGTCCACC CTCCTGGGAG CCTGGAGTGA CCGCGTGGGT CGCCGCCCAT 481 TGCTGGTGCT GGCCTCTCTT GGTCTGCTGC TCCAGGCCGT GGTGTCCATC TTTGTGGTGC 541 AACTACAACT GCACATCGGG TTCTTCGTGC TCGGCCGAGC CCTTTGTGCC CTTCTGGGAG 601 NTTTCAACGG CCTTCTTGCT GCTAGCTTTG CCTCTGTGGC TGATGTTAGC TCTAATCACA 661 GTCGCACCTT CCGGATGGCT CTGCTGGAAN CATGCATTGG TGTGGCCGGG ACCCTGGCAA 721 GCCTTCTTGG GGGTCACTGG CTCCNGGCCC AGGGTTATGC CNACCCTTTC TGGCTGGCTT 781 TGGCTGTGCT GATCGTTATG ANTCTATATG CAGCATTCTG TTTTGGTGAN ACAGNNNANG 841 ANCNNANNNN CNCNANGNTT TTTCACGCNN NNNCNTCACC NATCCNNNNN NCANCNNNNN 901 NNNNN //
Primer: pAxCALNLF1 (Pr0166) Region: insert 3' Sequence file: RDB18231_B0Fub.seq
>D07213A2_B0Fu_2_pAxCALNLF1_E06_15_ABI24.ab1 1 NNNNNNNNNN NNNANNNNNN CTGCTCGNNN CNTTAACCCT CACTAAAGGG AAGCGGCCGC 61 CCGGGTCGAC AGATCTGATG CNNNNNNACT GGGCTTTAGG TATGTAGTCG GTGTGGAGCC 121 ACTGCTTCTC ATGGCTTCTG GCAGCTCAGG AGTTCTGAGG AAACTGCTGG AACTCTGGGT 181 GTGGATTAAC CTTCTCCAGT ACCCCAATCA GAATGGCCGG GATAAAGAGG AGGCCAGCTC 241 CCAGGAGGAA GGGGAAGCCC TTCATGAAGT TCAGAGTGGC CGGGTAGAGT GAGTTGAAGA 301 TGCCGGAGGC CATCAACATG GCCAAGCTAT TCACACAGGC CACAGCCGAA AAGAGAGCAC 361 CCTGCTCCGA CTCGCTCACT AGCTTGGAGA GCTTGGCCCG GATCACAGGC GTAGTGACCA 421 ATGACAGGAA GAGTAATCCG TACCCTGTGA ACATGAGGGG TGTGATGGTT GCGAACGCAA 481 AGACCACCAT TCCCAGGATG TTGAAGGCCA GGCCAATCTC AGCCACCCAG GTGTCGGCCA 541 GGCAGAACTG CAGAAGTCTC AGGCCCAGCA GGCTGGTGAG GTAGGGTAAG TGCTGGGCTG 601 CTGANCCATA GCCAATCAGC TTGGAGTCCC AGCAGANGGG TGTGCTTAGC TCATAGAGCG 661 TCANGNTGTC TTGAGCCCCA AAGTGCACAG TGACCACCAC GAATATGGNC AATGANTACA 721 GGGCTAGATG CATCCTGNAC TTCTCTGGGG CTGGGANCAC ATACAGCTGG NCAATGGNTC 781 GGNGATGGCG GANCGNGAAA AGCCNCNNNG NNTTTGGCTC CTTCACTGNC TCACCAAAAC 841 ANANNGCTNN NNATANNNNC ATNNCGATCA NCACNNCCNA ANNNNGCCNN AANNGNNNNN 901 GNNTANNNNN NGNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18231_B0Fuc.seq
>D07213A2_B0Fu_2_SV40pro_ori_F_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN GCCTCNGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT 61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTGATTCTTC TGACACAACA GTCTCGAACT 121 TAAGGCTAGA GCCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG 181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC 241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG 301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG 421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT 481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA 541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA 601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 661 GGCGCGCATG CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA 721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC 781 NGNNCGCTAT CANGNCATAN CGTTGGCNAC CCGNNATATT GCTGAANANC TNGGCGGCGA 841 ANGGGNCNGA NCGCTTCCNC NNGCTTTACN NNATCGCCGC NCCCNATNCN NANCGNATCN 901 NCTTCNATCN NCTTCNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Inoue, K., Functional characterization of PCFT/HCP1 as the molecular entity of the carrier-mediated intestinal folate transport system in the rat model. Am. J. Physiol. Gastrointest. Liver Physiol. 294(3): G660-668 (2008). PMID 18174275. [link to RRC of NBRP]

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rat Slc46a1 and ortholog

External Database
rat Slc46a1

Reference sequence