RDB18230 - pCI-neo-hPCFT

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pCI-neo-hPCFT (#RDB18230)

Expression vector of human PCFT.

Clone info.	Expression vector of human proton-coupled folate transporter/heme carrier protein 1 (PCFT).
Comment	A reference nucleotide sequence for hPCFT cDNA is NM_080669.4.
Vector backbone	pCl-neo (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name	human SLC46A1 cDNA
Depositor\|Developer	Yuasa, Hiroaki \|

Sequence (full)	RDB18230zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Nakai Y. et al. J. Pharmacol. Exp. Ther. 322: 469-476, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Nakai Y. et al. J. Pharmacol. Exp. Ther. 322: 469-476, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Nakai Y. et al. J. Pharmacol. Exp. Ther. 322: 469-476, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Nakai Y. et al. J. Pharmacol. Exp. Ther. 322: 469-476, 2007)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。当該利用者によるリソースの利用が第三者の産業財産権をはじめとする一切の知的財産権を侵害しない旨の保証をせず、かつ商品性又は特定目的への適合性の保証をしない。また、当該利用者による本件成果有体物の使用、保有によって発生した如何なる結果、損害についても一切その責任を負担せず、かつ、如何なる損害賠償義務（直接、間接損害を問わない。）も負わない。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18230	pCI-neo-hPCFT	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-hPCFT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18230).

Reference section:

Nakai, Y., Inoue, K., Abe, N., Hatakeyama, M., Ohta, K.Y., Otagiri, M., Hayashi, Y., Yuasa, H., Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter. J. Pharmacol. Exp. Ther. 322 (2): 469-476 (2007). PMID 17475902. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18230_B0Fup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: chimeric intron 3-prime (Pr0444) Region: T7 pro, insert 5' Sequence file: RDB18230_B0Fua.seq
>D07213A1_B0Fu_1_chimeric intron3-prime_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNACNGCTCT TNGGCTAGAG TACTTAATAC GACTCACTAT 61 AGGCTAGCCT CGAGAATTCC GGAATTTCCT CGAGGTCGAC AGATCTGATT GCTCCGCCGC 121 GCACGCACAT GGAGGGGAGC GCGAGCCCCC CGGAAAAGCC CCGCGCCCGC CCTGCGGCTG 181 CCGTGCTGTG CCGGGGCCCG GTAGAGCCGC TGGTCTTCCT GGCCAACTTT GCCTTGGTCC 241 TGCAGGGCCC GCTCACCACG CAGTATCTGT GGCACCGCTT CAGCGCCGAC CTCGGCTACA 301 ATGGCACCCG CCAAAGGGGG GGCTGCAGCA ACCGCAGCGC GGACCCCACC ATGCAGGAAG 361 TGGAGACCCT TACCTCCCAC TGGACCCTCT ACATGAACGT GGGCGGCTTC CTGGTGGGGC 421 TCTTCTCGTC CACCCTGCTG GGAGCTTGGA GCGACAGTGT GGGCCGCCGC CCGCTGCTAG 481 TGCTGGCCTC GCTGGGCCTG CTGCTCCAGG CCCTAGTGTC CGTTTTTGTG GTGCAGCTGC 541 AGCTCCACGT CGGCTACTTC GTGCTGGGTC GCATCCTTTG TGCCCTCCTC GGCGACTTCG 601 GTGGCCTTCT GGCTGCTAGC TTTGCGTCCG TGGCAGATGT CAGCTCCAGT CGCAGCCGCA 661 CCTTCCGGAT GGCCCTGCTG GAAGCCAGCA TCGGGGTGGC TGGGATGCTG GCAAGCCTCC 721 TCNGGGGCCA CTNGCTCCNG GCCCAGGGTT ATGCCNACCN CTTCTGGCTG GCCTTGGNCT 781 TGCTGATANC CATGACTCTC TATGCAGCTT TCTGCTTTGG NGANANCTTA AAGGANNNNA 841 AGNNNNCCNN GCNCTTCNCG TNNCNNCACC ACCNNTNNNT NNNCCAGCNN NNNNNGGCNN 901 CNGCCCCNNN NNAANTCNNN NNNNNNTNNN NCNNNNNNNN NN //
Primer: pAxCALNLF1 (Pr0166) Region: T3 pro, insert 3' Sequence file: RDB18230_B0Fub.seq
>D07213A1_B0Fu_1_pAxCALNLF1_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNANNNNG TCTGCTNNNN NNNTAACCCT CACTAAAGGG AAGCGGCCGC 61 CCGGGTCGAC TCTAGACTAG TCTAGCGGCC GCGACCTGCA GATTTCAGGG GCTCTGGGGA 121 AACTGCTGGA ACTCGAGGTG AGGATCAGCC TTTTCCAGCA TCCCAATCAG AACAGCCGGG 181 ATGAGCAGGA GGCCAGCTCC CAGGAGGAAG GGGAACCCCT TCATAAAGTT CAGAGTGGCT 241 GGGTAGAGTG AGTTGAAGAT GCCGGAGGCC GTCAGCATGG CCAGGCTATT CACACAGGCC 301 ACAGCAGAAA AGAGAGCACC CTGCTCTGTC TCTCTCACCA GCTTGGAGAG TTTAGCCCGG 361 ATGACAGGTG TGATGACTAA TGACAGGAAA AGCAACCCAT ATCCTGTGAA CATGAGAGGC 421 GTGATAGTGG CAAAGGCAAA GACCACCATC CCCAGGATGT TGAAGGCCAG GCCGATCTCA 481 GCTACCCAGG CATCGGCCAG GCAGTACTGC AGGAGCTTCA GGGCCAGCAG GCTGGTGAGG 541 TAGGGGAGAT GCTGAGCTGC AGAACCATAG CCGATTAGTT TGGAGTCCCA GCAGAGGGGT 601 GTGCTTAGTT CATAAAGGGT TAAGATGTCC TGGGCCCCAA AGTGCACAGT GATCACCACG 661 AANATGGCCA GTGAGTAGAG GGCTAAATGT TTCCTGGACT TCTCTGGGGC GGGAGCCACA 721 TAGAGCTGGA CAATGGATCG GTGGTGACGG AACGTGAAGA NCCGGGNGGN NTTTGGCTCC 781 NTTANNGNCT CACCAAAGCA GAAAGCTGCA TAGAGNGTCA TGGNTATCAG CANGGNNNNN 841 GNNAGCCNGA NNGGNTTGGC ATNNCCNNNG NNCNNGNNCN NGNNNNCCNN NNNGNNNNTN 901 GNNANCATNC CANCCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR_KanR Sequence file: RDB18230_B0Fuc.seq
>D07213A1_B0Fu_1_SV40pro_ori_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC CTCNGCCTCT GNNCTATTCN NGAANNAGTG AGGANGCTTT 61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT GATTCTTCTG ACACAACAGT CTCGAACTTA 121 AGGCTAGAGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG 181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG 241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC 361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG 421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA 481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC 541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG 661 CGCGCATGCC CGACGGCGAG GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA 721 TCATGGTGGA NNATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG 781 ANCGCTATCA NGACATANCG TTGGCTACCC GNNATATTGC TGAAGANCTT GNCGGCNAAN 841 GGGNCNGANC GCTTCCNCGN GCTTTACNGN ATCGCCGCNN CCNANNCNNA NCGCATCNCN 901 TNNNNNCNNC NNTNNNNANN ANNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nakai, Y., Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter. J. Pharmacol. Exp. Ther. 322 (2): 469-476 (2007). PMID 17475902. [link to RRC of NBRP]

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human SLC46A1

External Database
human SLC46A1

Reference sequence