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pCI-neo-hPCFT

Expression vector of human PCFT.

Catalog number RDB18230
Resource name pCI-neo-hPCFT
Clone info. Expression vector of human proton-coupled folate transporter/heme carrier protein 1 (PCFT).
A reference nucleotide sequence for hPCFT cDNA is NM_080669.4.
Vector backbone pCl-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name human SLC46A1 cDNA
Depositor|Developer Yuasa, Hiroaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Nakai Y. et al. J. Pharmacol. Exp. Ther. 322: 469-476, 2007) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. This material is provided “as is” and without any warranty express or implied, including any warranty of merchantability or fitness for a particular purpose or that the use of the material will not infringe or violate any patent or other intellectual property rights of any third party or that the material will not pose a health or safety risk. The DEPOSITOR shall not be liable for any use of the material by the RECIPIENT, or for any loss, claim, damage, or liability of any kind or nature, which may arise from or in connection with this agreement or from the use, handling, or storage of the material.

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RDB18230 pCI-neo-hPCFT DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB18230zzk01.seq

Original reference

original Nakai, Y., Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter. J. Pharmacol. Exp. Ther. 322 (2): 469-476 (2007). PMID 17475902.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18230_B0Fup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: chimeric intron 3-prime
Region: T7 pro, insert 5'
Sequence file: RDB18230_B0Fua.seq check
>D07213A1_B0Fu_1_chimeric intron3-prime_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNACNGCTCT TNGGCTAGAG TACTTAATAC GACTCACTAT
   61 AGGCTAGCCT CGAGAATTCC GGAATTTCCT CGAGGTCGAC AGATCTGATT GCTCCGCCGC
  121 GCACGCACAT GGAGGGGAGC GCGAGCCCCC CGGAAAAGCC CCGCGCCCGC CCTGCGGCTG
  181 CCGTGCTGTG CCGGGGCCCG GTAGAGCCGC TGGTCTTCCT GGCCAACTTT GCCTTGGTCC
  241 TGCAGGGCCC GCTCACCACG CAGTATCTGT GGCACCGCTT CAGCGCCGAC CTCGGCTACA
  301 ATGGCACCCG CCAAAGGGGG GGCTGCAGCA ACCGCAGCGC GGACCCCACC ATGCAGGAAG
  361 TGGAGACCCT TACCTCCCAC TGGACCCTCT ACATGAACGT GGGCGGCTTC CTGGTGGGGC
  421 TCTTCTCGTC CACCCTGCTG GGAGCTTGGA GCGACAGTGT GGGCCGCCGC CCGCTGCTAG
  481 TGCTGGCCTC GCTGGGCCTG CTGCTCCAGG CCCTAGTGTC CGTTTTTGTG GTGCAGCTGC
  541 AGCTCCACGT CGGCTACTTC GTGCTGGGTC GCATCCTTTG TGCCCTCCTC GGCGACTTCG
  601 GTGGCCTTCT GGCTGCTAGC TTTGCGTCCG TGGCAGATGT CAGCTCCAGT CGCAGCCGCA
  661 CCTTCCGGAT GGCCCTGCTG GAAGCCAGCA TCGGGGTGGC TGGGATGCTG GCAAGCCTCC
  721 TCNGGGGCCA CTNGCTCCNG GCCCAGGGTT ATGCCNACCN CTTCTGGCTG GCCTTGGNCT
  781 TGCTGATANC CATGACTCTC TATGCAGCTT TCTGCTTTGG NGANANCTTA AAGGANNNNA
  841 AGNNNNCCNN GCNCTTCNCG TNNCNNCACC ACCNNTNNNT NNNCCAGCNN NNNNNGGCNN
901 CNGCCCCNNN NNAANTCNNN NNNNNNTNNN NCNNNNNNNN NN
//
Primer: pAxCALNLF1
Region: T3 pro, insert 3'
Sequence file: RDB18230_B0Fub.seq check
>D07213A1_B0Fu_1_pAxCALNLF1_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNANNNNG TCTGCTNNNN NNNTAACCCT CACTAAAGGG AAGCGGCCGC
   61 CCGGGTCGAC TCTAGACTAG TCTAGCGGCC GCGACCTGCA GATTTCAGGG GCTCTGGGGA
  121 AACTGCTGGA ACTCGAGGTG AGGATCAGCC TTTTCCAGCA TCCCAATCAG AACAGCCGGG
  181 ATGAGCAGGA GGCCAGCTCC CAGGAGGAAG GGGAACCCCT TCATAAAGTT CAGAGTGGCT
  241 GGGTAGAGTG AGTTGAAGAT GCCGGAGGCC GTCAGCATGG CCAGGCTATT CACACAGGCC
  301 ACAGCAGAAA AGAGAGCACC CTGCTCTGTC TCTCTCACCA GCTTGGAGAG TTTAGCCCGG
  361 ATGACAGGTG TGATGACTAA TGACAGGAAA AGCAACCCAT ATCCTGTGAA CATGAGAGGC
  421 GTGATAGTGG CAAAGGCAAA GACCACCATC CCCAGGATGT TGAAGGCCAG GCCGATCTCA
  481 GCTACCCAGG CATCGGCCAG GCAGTACTGC AGGAGCTTCA GGGCCAGCAG GCTGGTGAGG
  541 TAGGGGAGAT GCTGAGCTGC AGAACCATAG CCGATTAGTT TGGAGTCCCA GCAGAGGGGT
  601 GTGCTTAGTT CATAAAGGGT TAAGATGTCC TGGGCCCCAA AGTGCACAGT GATCACCACG
  661 AANATGGCCA GTGAGTAGAG GGCTAAATGT TTCCTGGACT TCTCTGGGGC GGGAGCCACA
  721 TAGAGCTGGA CAATGGATCG GTGGTGACGG AACGTGAAGA NCCGGGNGGN NTTTGGCTCC
  781 NTTANNGNCT CACCAAAGCA GAAAGCTGCA TAGAGNGTCA TGGNTATCAG CANGGNNNNN
  841 GNNAGCCNGA NNGGNTTGGC ATNNCCNNNG NNCNNGNNCN NGNNNNCCNN NNNGNNNNTN
901 GNNANCATNC CANCCNNNNN NNNNN
//
Primer: SV40pro_ori_F
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB18230_B0Fuc.seq check
>D07213A1_B0Fu_1_SV40pro_ori_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC CTCNGCCTCT GNNCTATTCN NGAANNAGTG AGGANGCTTT
   61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT GATTCTTCTG ACACAACAGT CTCGAACTTA
  121 AGGCTAGAGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
  181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
  241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
  301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
  361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
  421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
  481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
  541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
  601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
  661 CGCGCATGCC CGACGGCGAG GNTCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
  721 TCATGGTGGA NNATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG
  781 ANCGCTATCA NGACATANCG TTGGCTACCC GNNATATTGC TGAAGANCTT GNCGGCNAAN
  841 GGGNCNGANC GCTTCCNCGN GCTTTACNGN ATCGCCGCNN CCNANNCNNA NCGCATCNCN
901 TNNNNNCNNC NNTNNNNANN ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nakai, Y., Functional characterization of human proton-coupled folate transporter/heme carrier protein 1 heterologously expressed in mammalian cells as a folate transporter. J. Pharmacol. Exp. Ther. 322 (2): 469-476 (2007). PMID 17475902.

2020.11.16

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