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pMAK463

Bacterial expression vector of nanobody to recognize mouse kappa chain (NB alpha Ms-IgK).

Catalog number RDB18228
Resource name pMAK463
Clone info. Bacterial expression vector of nanobody to recognize mouse kappa chain (NB alpha Ms-IgK) tagged with AviTAG and 6x His at C-terminus, see Fig. 1E of Iwaki, T. et al., Protein Expr. Purif., 170: 105607, 2020. This clone is a vector that enables secretory expression of nanobodies in E. coli.
Comment Expression was confirmed by the depositor with coomassie brilliant blue stain, Western blot.
Vector backbone pMal-T-Avi-His/BirA (plasmid)
Selectable markers Ampicillin
Depositor|Developer Iwaki, Takayuki |
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External Database
            Reference sequence
              
             
            Sequence (full) RDB18228zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020)
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020)。
            付加的提供条件：
            本件リソースの使用は学術機関での学術研究に限る。
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            RDB18228 pMAK463 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Iwaki, T., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Iwaki, T., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022.

            2022.10.11

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