Resource data sheet
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pMAK462 (#RDB18227)

Bacterial expression vector of nanobody to recognize mouse/rat IgG1-Fc (NB alpha Ms/Rt-IgG1-Fc).

Clone info. Bacterial expression vector of nanobody to recognize mouse/rat IgG1-Fc (NB alpha Ms/Rt-IgG1-Fc) tagged with AviTAG and 6x His at C-terminus, see Fig. 1D of Iwaki, T. et al., Protein Expr. Purif., 170: 105607, 2020. This clone is a vector that enables secretory expression of nanobodies in E. coli.
Comment Expression was confirmed by the depositor with coomassie brilliant blue stain, Western blot.
Vector backbone pMal-T-Avi-His/BirA (plasmid)
Selectable markers Ampicillin
Depositor|Developer Iwaki, Takayuki |
 
Sequence (full) RDB18227zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18227 pMAK462 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMAK462 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18227).

Reference section:

Iwaki, T., Hara, K., Umemura, K., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Iwaki, T., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022. [link to RRC of NBRP]