Bacterial expression vector of nanobody to recognize mouse/rat IgG1-Fc (NB alpha Ms/Rt-IgG1-Fc).
Clone info. | Bacterial expression vector of nanobody to recognize mouse/rat IgG1-Fc (NB alpha Ms/Rt-IgG1-Fc) tagged with AviTAG and 6x His at C-terminus, see Fig. 1D of Iwaki, T. et al., Protein Expr. Purif., 170: 105607, 2020. This clone is a vector that enables secretory expression of nanobodies in E. coli. |
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Comment | Expression was confirmed by the depositor with coomassie brilliant blue stain, Western blot. |
Vector backbone | pMal-T-Avi-His/BirA (plasmid) |
Selectable markers | Ampicillin |
Depositor|Developer | Iwaki, Takayuki | |
Sequence (full) | RDB18227zzk01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested.(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Iwaki, T. et al. Protein Expr. Purif. 170:105607, 2020)。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18227 | pMAK462 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pMAK462 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18227). |
Reference section:
Iwaki, T., Hara, K., Umemura, K., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Iwaki, T., Nanobody production can be simplified by direct secretion from Escherichia coli. Protein Expr. Purif. 170: 105607 (2020). PMID 32062022. [link to RRC of NBRP] |
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