Resource data sheet
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pShuttle-CMV/AsHSF1-Flag (#RDB18207)

Expression vector for anole lizard HSF1.

Clone info. Expression vector for anole lizard heat shock transcription factor 1 (HSF1) tagged with FLAG at C-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pShuttle-CMV (plasmid)
Size of vector backbone 7.5 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name Anole lizard (Anolis sagrei) HSF1 cDNA
Depositor|Developer Nakai, Akira |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takii, R. et al. EMBO J. 38(24), e102566, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takii, R. et al. EMBO J. 38(24), e102566, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18207 pShuttle-CMV/AsHSF1-Flag Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pShuttle-CMV/AsHSF1-Flag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18207).

Reference section:

Takii, R., Fujimoto, M., Matsumoto, M., Srivastava, P., Katiyar, A., Nakayama, K.I., Nakai, A., The pericentromeric protein shugoshin 2 cooperates with HSF1 in heat shock response and RNA Pol II recruitment. EMBO J. 38 (24): e102566 (2019). PMID 31657478. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Takii, R., The pericentromeric protein shugoshin 2 cooperates with HSF1 in heat shock response and RNA Pol II recruitment. EMBO J. 38 (24): e102566 (2019). PMID 31657478. [link to RRC of NBRP]