Expression vector of human ZBTB24 deletion of ZF domain (294 to 512).
Clone info. | Expression vector of human zinc finger and BTB domain containing 24 (ZBTB24) deletion of ZF domain (294 to 512), fused with N-terminal EGFP, CMV promoter. |
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Comment | Expression was confirmed by the depositor with western blotting and fluorescence microscope. Mutagenesis: forward primer (5'- AGCAAGGAGAAGCATGCTTC -3'), reverse primer (5'- CTCAGGGCCTCCAGGGCG -3'). |
Vector backbone | pEGFP-C1 (plasmid) |
Size of vector backbone | 4.7 kb |
Selectable markers | Kanamycin (E. coli), Neomycin (mammalian cells) |
Gene/insert name | human ZBTB24 cDNA |
Depositor|Developer | Unoki, Motoko | |
Sequence (partial) | RDB18187pka01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18187 | EGFP-ZBTB24 ΔZF (human) | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The EGFP-ZBTB24 ΔZF (human) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18187). |
Reference section:
Aktar, S., Sasaki, H., Unoki, M., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
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