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EGFP-ZBTB24 ΔBTB (human) (#RDB18185)

Expression vector of human ZBTB24 deletion of BTB domain (37 to 133).

Clone info. Expression vector of human zinc finger and BTB domain containing 24 (ZBTB24) deletion of BTB domain (37 to 133), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Mutagenesis: forward primer (5'- TCCCCAAAGCCAACAACTTT -3'), reverse primer (5'- GAGGAAGCCTTTCTTCCTC -3').
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name human ZBTB24 cDNA
Depositor|Developer Unoki, Motoko |
 
Sequence (partial) RDB18185pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18185 EGFP-ZBTB24 ΔBTB (human) Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The EGFP-ZBTB24 ΔBTB (human) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18185).

Reference section:

Aktar, S., Sasaki, H., Unoki, M., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP]