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EGFP-ZBTB24 WT (human)

Expression vector of human ZBTB24 wild type.

Catalog number RDB18184
Resource name EGFP-ZBTB24 WT (human)
Clone info. Expression vector of human zinc finger and BTB domain containing 24 (ZBTB24) wild type, fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name human ZBTB24 cDNA
Depositor|Developer Unoki, Motoko |
Other clones in our bank

External Database
human ZBTB24

          Reference sequence
            
           
          Sequence (partial) RDB18184pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013 )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18184 EGFP-ZBTB24 WT (human) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18184_B0Cnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3', insert 5'
          Sequence file: RDB18184_B0Cna.seq check
          >D06978C8_B0Cn_1_EGFP-C2_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNC NCNTGGTCCT GCTGGANTTC GTGANCGCCG CCGGGNTCAC
             61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGCAGAAA CATCGCCAGA
            121 GCCTTCTGGG CAGCTTGTTG TACACTCAGA CGCTCACAGT GACACTGTGC TGGCCAGTTT
            181 TGAGGATCAG AGGAAGAAAG GCTTCCTCTG TGACATTACT TTAATCGTGG AGAATGTACA
            241 TTTCCGGGCC CACAAAGCCT TACTTGCTGC CAGTAGTGAA TACTTCTCAA TGATGTTTGC
            301 AGAAGAGGGG GAAATCGGCC AATCCATTTA TATGCTGGAA GGCATGGTTG CAGACACCTT
            361 TGGTATCCTG CTGGAATTTA TCTACACAGG TTATCTCCAT GCCAGTGAGA AAAGTACAGA
            421 ACAAATCCTG GCTACTGCTC AGTTCTTAAA AGTCTATGAC CTGGTAAAGG CTTACACAGA
            481 CTTCCAAAAT AATCATAGCT CCCCAAAGCC AACAACTTTG AACACTGCTG GTGCCCCAGT
            541 GGTTGTTATC TCTAATAAGA AAAACGATCC TCCAAAGCGG AAACGGGGAA GACCAAAAAA
            601 AGTCAATACA TTGCAGGAGG AGAAATCAGA ACTGGCTGCA GAGGAAGAAA TACAGTTAAG
            661 AGTGAACAAT TCAGTTCAGA ATAGACAAAA CTTTGTGGTT AAAGGAGACA GTGGTGTACT
            721 GAATGAGCAA ATTGCAGCAA AAGAAAAGGA AGAATCGGAN CCTACTTGTG AGCCAAGTAG
            781 AGAGGANGAA ATGCCAGTTG ANAAAGANGA GAACTATGAT CCCAAGACCG ANGATGGCCA
            841 GGCAAGCCAG ANTCGATACA GCAAGCGGAN GANTTGGAGA TCCGTCAAAC TTAAANANTA
            901 CNAACTTGTT GGGGATCAAG ANGANNATGG TTCANCCNAG ANGNNCTGTG GNANGNAGAA
            961 AGNGCCCNNN NNGNCNNGAN GNCNNCNNTN NNNNTGNGGN NNNGNTCNTT NNNTANNNTC
          1021 ACTTTTTANC NNTNNANNNN NNNNNN
          //
          Primer: SV40polyA_R2
          Region: SV40 p(A), insert 3'
          Sequence file: RDB18184_B0Cnb.seq check
          >D06978C8_B0Cn_1_SV40polyA_R2_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNGNNNNNN ANNNGNNNNN NNNNACNATT GCATTCATTT TATGTTTCAG
             61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
            121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
            181 AGAATTCAAA CGTGTTTACA GGTCAGCTCT GCTCCTGGCC AAGTGGCGTT GGCTGGGGCA
            241 CGTGGTGAGT GGGTGGTGGC GGACCAGGCT CCTGTGTCAG CTGCAGGTGC TGAGCTGGAT
            301 CTGAAGTACT TGTAGCTAAA TGGAGCTCCT CTGTTTGCTC TTGATGGGCA TGAAGATGTT
            361 CCAGTGTTTC CTTGGACAGA GTGATCACGT GCACTGGCTC TGTTTGTGAG GGCCCCATCT
            421 GGCTTTCAAT CATATTGAGG CTCTGAATGT GTTCTGTTTG CTCCTGTTGA GCTGAAAGAA
            481 TTAAATTCTG CAGTTGCTCT GGCTGCTGCG TGAGCAGGGT AAGATTAGCA GCCTGGTCTG
            541 CAGTCATGTT TTGGGAACTC TCTGCAGTCA CAATGCTGAT TCCCTGGCTA GGACCGGGCA
            601 TGAAATTGAT GTTATGTACA GAATCGGTTA CGAGAAGCTG AATTTCCTGC TCTCCCGAGG
            661 TAGAGAGTTG ATATGGCTGT AGCTGAAGAA TATTCCTGAC CTCTTCTGTA TTACTACTGC
            721 CAGAAATGCT GCTGGCATCT GAAGCATGCT TCTCCTTGCT ATGAATTTTC AAGTGAGCCT
            781 TCNAGTTGTC TAAGCGAGCA AACTGTAAGT TACACTCAGG GCAGGAGAAA GGCTTCTTGC
            841 CAGTGTGTAG AATGCAGTGT CTCCTTTTGG CACTGGANTC AGANANNGNN TTGNCNCNAA
            901 TGNCNCAGGA NTATGGCTTT TCTCCTCNAT GNANTCTGAN GTGGGTCTNA ANANAACTCT
            961 TTGCTGTGAA ANANTTNNNN NANANTNCNN NNGTAAATGG NTTCNNNNNN GNNNGTGNNC
          1021 NCANNNGNTT CNTTNNNNNA NNNNNNTNNN NNNNNTNNNN NNNNNN
          //
          Primer: hZBTB24-M_F1
          Region: insert mid
          Sequence file: RDB18184_B0Cnc.seq check
          >D06978C8_B0Cn_1_hZBTB24-M_F1_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT GNNGGNNGAN AAANCAGAAC TGGCTGCAGA GGAAGAAATA
             61 CAGTTAAGAG TGAACAATTC AGTTCAGAAT AGACAAAACT TTGTGGTTAA AGGAGACAGT
            121 GGTGTACTGA ATGAGCAAAT TGCAGCAAAA GAAAAGGAAG AATCGGAGCC TACTTGTGAG
            181 CCAAGTAGAG AGGAGGAAAT GCCAGTTGAA AAAGATGAGA ACTATGATCC CAAGACCGAG
            241 GATGGCCAGG CAAGCCAGAG TCGATACAGC AAGCGGAGGA TTTGGAGATC CGTCAAACTT
            301 AAAGATTACA AACTTGTTGG GGATCAAGAG GACCATGGTT CAGCCAAGAG GATCTGTGGA
            361 AGGAGAAAGC GCCCTGGAGG CCCTGAGGCC CGCTGTAAAG ACTGTGGCAA GGTCTTTAAG
            421 TACAATCACT TTTTAGCAAT CCACCAGAGG AGCCACACAG GGGAGCGACC TTTCAAATGT
            481 AATGAGTGTG GAAAAGGCTT TGCCCAGAAG CACTCGCTAC AGGTCCACAC CAGGATGCAC
            541 ACAGGCGAGC GGCCGTACAC CTGCACCGTG TGCAGCAAGG CTCTGACCAC CAAGCACTCA
            601 CTGCTGGAGC ACATGAGCCT GCACTCAGGA CAGAAGTCTT TTACCTGTGA TCAATGCGGA
            661 AAATATTTCA GCCAGAACAG ACAGCTAAAG AGCCATTACC GAGTTCATAC AGGCCACTCA
            721 TTACCGGAAT GCAAAGACTG CCATCGCAAA TTCATGGATG TGTCTCAGCT AAAGAAACAT
            781 CTGCGAACAC ACACAGGTGA NAAGCCATTT ACTTGTGAAA TCTGTGGCAA ATCTTTCACA
            841 GCAAAGAGTT CTCTTCAGAN CCACATCANA NTCCATCGAG GANAAAAGCC ATACTCCNGT
            901 GGCATTTGTG GNAAATCCTT CTCTGANTNC NGNGNCNAAA GGANANNCTG NNNTCNACNC
            961 ACTGNNAAGA AGCCNTTCNC CTGNNNNNAN NGNNNCNTAN NNTTNNNNCG CNNNNNNNNN
          1021 N
          //
          Primer: hZBTB24-M_R1
          Region: insert mid
          Sequence file: RDB18184_B0Cnd.seq check
          >D06978C8_B0Cn_1_hZBTB24-M_R1_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNGTTANGT ACNGAATCGG TTACGAGAAG CTGAATTTCC
             61 TGCTCTCCCG AGGTAGAGAG TTGATATGGC TGTAGCTGAA GAATATTCCT GACCTCTTCT
            121 GTATTACTAC TGCCAGAAAT GCTGCTGGCA TCTGAAGCAT GCTTCTCCTT GCTATGAATT
            181 TTCAAGTGAG CCTTCAAGTT GTCTAAGCGA GCAAACTGTA AGTTACACTC AGGGCAGGAG
            241 AAAGGCTTCT TGCCAGTGTG TAGAATGCAG TGTCTCCTTT TGGCACTGGA GTCAGAGAAG
            301 GATTTGCCAC AAATGCCACA GGAGTATGGC TTTTCTCCTC GATGGATTCT GATGTGGGTC
            361 TGAAGAGAAC TCTTTGCTGT GAAAGATTTG CCACAGATTT CACAAGTAAA TGGCTTCTCA
            421 CCTGTGTGTG TTCGCAGATG TTTCTTTAGC TGAGACACAT CCATGAATTT GCGATGGCAG
            481 TCTTTGCATT CCGGTAATGA GTGGCCTGTA TGAACTCGGT AATGGCTCTT TAGCTGTCTG
            541 TTCTGGCTGA AATATTTTCC GCATTGATCA CAGGTAAAAG ACTTCTGTCC TGAGTGCAGG
            601 CTCATGTGCT CCAGCAGTGA GTGCTTGGTG GTCAGAGCCT TGCTGCACAC GGTGCAGGTG
            661 TACGGCCGCT CGCCTGTGTG CATCCTGGTG TGGACCTGTA GCGAGTGCTT CTGGGCAAAG
            721 CCTTTTCCAC ACTCATTACA TTTGAAAGGT CGCTCCCCTG TGTGGCTCCT CTGGTGGANT
            781 GCTAAAAAGT GANTGTACTT AAAGACCTTG CCACAGTCTT TACAGCGGGC CTCANNGCCT
            841 CCAGGGCGCT TTCTCCTTCC ACAGATCCTC TTGGCTGAAC CATGGNCCTC TTGATCCCCA
            901 ACAAGTTTGT AATCNTTAAN TTTGANGGAN CTCCAAATCC TCCGCTTGCT GNATCGACTC
            961 NNGNTTGNCT GNNCNTCCNC NGNCNNGGNN TCNTANNNNT CATCTTTTTC NNNNGNNATT
          1021 TNCNNNNNNN NNANNTNGNN NNNNNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro, EGFP 5'
          Sequence file: RDB18184_B0Cne.seq check
          >D06978C8_B0Cn_1_CMV_Foward_E09_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
            121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
            181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
            241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
            301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
            361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
            421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
            481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
            541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
            601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
            661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
            721 AGCACCCAGT CCGCCCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
            781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGNCG AGCTGTACAA GTCCGGANTC
            841 NNATCTATGG CAGAAACATC GCCNGANCCT TCTGGGCAGC TTGTTGTACA CTCNGACGCT
            901 CACNGNGACA CTGNGCTNGN CAGTTTTGAN GATCANANGA AGAAANGNTT NCNCTGTGAN
            961 NTTACTTNNA TCGNNNNNAA TGNANATTTT NCNGGNCCCN NAAAGCCTTA CNTNGCNGNC
          1021 NNNANNGNAN NNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR_KanR
          Sequence file: RDB18184_B0Cnf.seq check
          >D06978C8_B0Cn_1_SV40pro_ori_F_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN CGCCTCGNCC TCTGANCTAT TCNNGAAGTA GTGAGGAGGC
             61 TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG
            121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
            181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
            241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
            301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
            361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
            421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
            481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
            541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
            601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
            661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
            721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA
            781 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGNC GGCGAATGNG CTGANCGCTT
            841 CCTCGTGCTT TACNGTATCG CCGCTCNNGA TTCGCAGCGC ATCGCCNTCN ATCGCCTTCT
            901 TGANGAGTTC TTCTGANCGG GANTCTGGGG NNCGAAATGA NCNANCNAGC NACGCCCNNN
            961 CNNGCCNTCN CNANNNTTCN NNNNCNNCNG NCNNCNTTCN NNGAANNNNN GGNNNNNNNN
          1021 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl