Please review the QC test results indicated by icon below as well as clone information before placing your order.

EGFP-ZBTB24 WT (human) (#RDB18184)

Expression vector of human ZBTB24 wild type.

Clone info.	Expression vector of human zinc finger and BTB domain containing 24 (ZBTB24) wild type, fused with N-terminal EGFP, CMV promoter.
Comment	Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name	human ZBTB24 cDNA
Depositor|Developer	Unoki, Motoko |

 

Sequence (partial)	RDB18184pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013)

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18184	EGFP-ZBTB24 WT (human)	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The EGFP-ZBTB24 WT (human) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18184).

Reference section:

Nitta, H., Unoki, M., Ichiyanagi, K., Kosho, T., Shigemura, T., Takahashi, H., Velasco, G., Francastel, C., Picard, C., Kubota, T., Sasaki, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18184_B0Cnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3', insert 5' Sequence file: RDB18184_B0Cna.seq
>D06978C8_B0Cn_1_EGFP-C2_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNC NCNTGGTCCT GCTGGANTTC GTGANCGCCG CCGGGNTCAC    61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT ATGGCAGAAA CATCGCCAGA   121 GCCTTCTGGG CAGCTTGTTG TACACTCAGA CGCTCACAGT GACACTGTGC TGGCCAGTTT   181 TGAGGATCAG AGGAAGAAAG GCTTCCTCTG TGACATTACT TTAATCGTGG AGAATGTACA   241 TTTCCGGGCC CACAAAGCCT TACTTGCTGC CAGTAGTGAA TACTTCTCAA TGATGTTTGC   301 AGAAGAGGGG GAAATCGGCC AATCCATTTA TATGCTGGAA GGCATGGTTG CAGACACCTT   361 TGGTATCCTG CTGGAATTTA TCTACACAGG TTATCTCCAT GCCAGTGAGA AAAGTACAGA   421 ACAAATCCTG GCTACTGCTC AGTTCTTAAA AGTCTATGAC CTGGTAAAGG CTTACACAGA   481 CTTCCAAAAT AATCATAGCT CCCCAAAGCC AACAACTTTG AACACTGCTG GTGCCCCAGT   541 GGTTGTTATC TCTAATAAGA AAAACGATCC TCCAAAGCGG AAACGGGGAA GACCAAAAAA   601 AGTCAATACA TTGCAGGAGG AGAAATCAGA ACTGGCTGCA GAGGAAGAAA TACAGTTAAG   661 AGTGAACAAT TCAGTTCAGA ATAGACAAAA CTTTGTGGTT AAAGGAGACA GTGGTGTACT   721 GAATGAGCAA ATTGCAGCAA AAGAAAAGGA AGAATCGGAN CCTACTTGTG AGCCAAGTAG   781 AGAGGANGAA ATGCCAGTTG ANAAAGANGA GAACTATGAT CCCAAGACCG ANGATGGCCA   841 GGCAAGCCAG ANTCGATACA GCAAGCGGAN GANTTGGAGA TCCGTCAAAC TTAAANANTA   901 CNAACTTGTT GGGGATCAAG ANGANNATGG TTCANCCNAG ANGNNCTGTG GNANGNAGAA   961 AGNGCCCNNN NNGNCNNGAN GNCNNCNNTN NNNNTGNGGN NNNGNTCNTT NNNTANNNTC 1021 ACTTTTTANC NNTNNANNNN NNNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: SV40 p(A), insert 3' Sequence file: RDB18184_B0Cnb.seq
>D06978C8_B0Cn_1_SV40polyA_R2_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNN ANNNGNNNNN NNNNACNATT GCATTCATTT TATGTTTCAG    61 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG   121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC   181 AGAATTCAAA CGTGTTTACA GGTCAGCTCT GCTCCTGGCC AAGTGGCGTT GGCTGGGGCA   241 CGTGGTGAGT GGGTGGTGGC GGACCAGGCT CCTGTGTCAG CTGCAGGTGC TGAGCTGGAT   301 CTGAAGTACT TGTAGCTAAA TGGAGCTCCT CTGTTTGCTC TTGATGGGCA TGAAGATGTT   361 CCAGTGTTTC CTTGGACAGA GTGATCACGT GCACTGGCTC TGTTTGTGAG GGCCCCATCT   421 GGCTTTCAAT CATATTGAGG CTCTGAATGT GTTCTGTTTG CTCCTGTTGA GCTGAAAGAA   481 TTAAATTCTG CAGTTGCTCT GGCTGCTGCG TGAGCAGGGT AAGATTAGCA GCCTGGTCTG   541 CAGTCATGTT TTGGGAACTC TCTGCAGTCA CAATGCTGAT TCCCTGGCTA GGACCGGGCA   601 TGAAATTGAT GTTATGTACA GAATCGGTTA CGAGAAGCTG AATTTCCTGC TCTCCCGAGG   661 TAGAGAGTTG ATATGGCTGT AGCTGAAGAA TATTCCTGAC CTCTTCTGTA TTACTACTGC   721 CAGAAATGCT GCTGGCATCT GAAGCATGCT TCTCCTTGCT ATGAATTTTC AAGTGAGCCT   781 TCNAGTTGTC TAAGCGAGCA AACTGTAAGT TACACTCAGG GCAGGAGAAA GGCTTCTTGC   841 CAGTGTGTAG AATGCAGTGT CTCCTTTTGG CACTGGANTC AGANANNGNN TTGNCNCNAA   901 TGNCNCAGGA NTATGGCTTT TCTCCTCNAT GNANTCTGAN GTGGGTCTNA ANANAACTCT   961 TTGCTGTGAA ANANTTNNNN NANANTNCNN NNGTAAATGG NTTCNNNNNN GNNNGTGNNC 1021 NCANNNGNTT CNTTNNNNNA NNNNNNTNNN NNNNNTNNNN NNNNNN //
Primer: hZBTB24-M_F1 (Pr0849) Region: insert mid Sequence file: RDB18184_B0Cnc.seq
>D06978C8_B0Cn_1_hZBTB24-M_F1_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT GNNGGNNGAN AAANCAGAAC TGGCTGCAGA GGAAGAAATA    61 CAGTTAAGAG TGAACAATTC AGTTCAGAAT AGACAAAACT TTGTGGTTAA AGGAGACAGT   121 GGTGTACTGA ATGAGCAAAT TGCAGCAAAA GAAAAGGAAG AATCGGAGCC TACTTGTGAG   181 CCAAGTAGAG AGGAGGAAAT GCCAGTTGAA AAAGATGAGA ACTATGATCC CAAGACCGAG   241 GATGGCCAGG CAAGCCAGAG TCGATACAGC AAGCGGAGGA TTTGGAGATC CGTCAAACTT   301 AAAGATTACA AACTTGTTGG GGATCAAGAG GACCATGGTT CAGCCAAGAG GATCTGTGGA   361 AGGAGAAAGC GCCCTGGAGG CCCTGAGGCC CGCTGTAAAG ACTGTGGCAA GGTCTTTAAG   421 TACAATCACT TTTTAGCAAT CCACCAGAGG AGCCACACAG GGGAGCGACC TTTCAAATGT   481 AATGAGTGTG GAAAAGGCTT TGCCCAGAAG CACTCGCTAC AGGTCCACAC CAGGATGCAC   541 ACAGGCGAGC GGCCGTACAC CTGCACCGTG TGCAGCAAGG CTCTGACCAC CAAGCACTCA   601 CTGCTGGAGC ACATGAGCCT GCACTCAGGA CAGAAGTCTT TTACCTGTGA TCAATGCGGA   661 AAATATTTCA GCCAGAACAG ACAGCTAAAG AGCCATTACC GAGTTCATAC AGGCCACTCA   721 TTACCGGAAT GCAAAGACTG CCATCGCAAA TTCATGGATG TGTCTCAGCT AAAGAAACAT   781 CTGCGAACAC ACACAGGTGA NAAGCCATTT ACTTGTGAAA TCTGTGGCAA ATCTTTCACA   841 GCAAAGAGTT CTCTTCAGAN CCACATCANA NTCCATCGAG GANAAAAGCC ATACTCCNGT   901 GGCATTTGTG GNAAATCCTT CTCTGANTNC NGNGNCNAAA GGANANNCTG NNNTCNACNC   961 ACTGNNAAGA AGCCNTTCNC CTGNNNNNAN NGNNNCNTAN NNTTNNNNCG CNNNNNNNNN 1021 N //
Primer: hZBTB24-M_R1 (Pr0850) Region: insert mid Sequence file: RDB18184_B0Cnd.seq
>D06978C8_B0Cn_1_hZBTB24-M_R1_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGTTANGT ACNGAATCGG TTACGAGAAG CTGAATTTCC    61 TGCTCTCCCG AGGTAGAGAG TTGATATGGC TGTAGCTGAA GAATATTCCT GACCTCTTCT   121 GTATTACTAC TGCCAGAAAT GCTGCTGGCA TCTGAAGCAT GCTTCTCCTT GCTATGAATT   181 TTCAAGTGAG CCTTCAAGTT GTCTAAGCGA GCAAACTGTA AGTTACACTC AGGGCAGGAG   241 AAAGGCTTCT TGCCAGTGTG TAGAATGCAG TGTCTCCTTT TGGCACTGGA GTCAGAGAAG   301 GATTTGCCAC AAATGCCACA GGAGTATGGC TTTTCTCCTC GATGGATTCT GATGTGGGTC   361 TGAAGAGAAC TCTTTGCTGT GAAAGATTTG CCACAGATTT CACAAGTAAA TGGCTTCTCA   421 CCTGTGTGTG TTCGCAGATG TTTCTTTAGC TGAGACACAT CCATGAATTT GCGATGGCAG   481 TCTTTGCATT CCGGTAATGA GTGGCCTGTA TGAACTCGGT AATGGCTCTT TAGCTGTCTG   541 TTCTGGCTGA AATATTTTCC GCATTGATCA CAGGTAAAAG ACTTCTGTCC TGAGTGCAGG   601 CTCATGTGCT CCAGCAGTGA GTGCTTGGTG GTCAGAGCCT TGCTGCACAC GGTGCAGGTG   661 TACGGCCGCT CGCCTGTGTG CATCCTGGTG TGGACCTGTA GCGAGTGCTT CTGGGCAAAG   721 CCTTTTCCAC ACTCATTACA TTTGAAAGGT CGCTCCCCTG TGTGGCTCCT CTGGTGGANT   781 GCTAAAAAGT GANTGTACTT AAAGACCTTG CCACAGTCTT TACAGCGGGC CTCANNGCCT   841 CCAGGGCGCT TTCTCCTTCC ACAGATCCTC TTGGCTGAAC CATGGNCCTC TTGATCCCCA   901 ACAAGTTTGT AATCNTTAAN TTTGANGGAN CTCCAAATCC TCCGCTTGCT GNATCGACTC   961 NNGNTTGNCT GNNCNTCCNC NGNCNNGGNN TCNTANNNNT CATCTTTTTC NNNNGNNATT 1021 TNCNNNNNNN NNANNTNGNN NNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, EGFP 5' Sequence file: RDB18184_B0Cne.seq
>D06978C8_B0Cn_1_CMV_Foward_E09_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG NNNNNNNGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG   121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC   181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG   241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG   301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC   361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG   421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC   481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG   541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC   601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC   661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG   721 AGCACCCAGT CCGCCCTGAN CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG   781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGNCG AGCTGTACAA GTCCGGANTC   841 NNATCTATGG CAGAAACATC GCCNGANCCT TCTGGGCAGC TTGTTGTACA CTCNGACGCT   901 CACNGNGACA CTGNGCTNGN CAGTTTTGAN GATCANANGA AGAAANGNTT NCNCTGTGAN   961 NTTACTTNNA TCGNNNNNAA TGNANATTTT NCNGGNCCCN NAAAGCCTTA CNTNGCNGNC 1021 NNNANNGNAN NNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR_KanR Sequence file: RDB18184_B0Cnf.seq
>D06978C8_B0Cn_1_SV40pro_ori_F_F09_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN CGCCTCGNCC TCTGANCTAT TCNNGAAGTA GTGAGGAGGC    61 TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG   121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT   181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC   241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT   301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT   361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA   421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT   481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG   541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA   601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA   661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA   721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA   781 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGNC GGCGAATGNG CTGANCGCTT   841 CCTCGTGCTT TACNGTATCG CCGCTCNNGA TTCGCAGCGC ATCGCCNTCN ATCGCCTTCT   901 TGANGAGTTC TTCTGANCGG GANTCTGGGG NNCGAAATGA NCNANCNAGC NACGCCCNNN   961 CNNGCCNTCN CNANNNTTCN NNNNCNNCNG NCNNCNTTCN NNGAANNNNN GGNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126. [link to RRC of NBRP]

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• human ZBTB24

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External Database
human ZBTB24

• in KEGG Gene
• in KEGG Orthology
• in NCBI Gene

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