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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

EGFP-ZBTB24 WT (mouse)

Expression vector of mouse Zbtb24 wild type.

Catalog number RDB18170
Resource name EGFP-ZBTB24 WT (mouse)
Clone info. Expression vector of mouse zinc finger and BTB domain containing 24 (Zbtb24) wild type, fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name mouse Zbtb24 cDNA
Depositor|Developer Unoki, Motoko |
Other clones in our bank

External Database
mouse Zbtb24

          Reference sequence
            
           
          Sequence (partial) RDB18170pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013 )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18170 EGFP-ZBTB24 WT (mouse) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18170_B0Cnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2
          Region: EGFP 3', insert 5'
          Sequence file: RDB18170_B0Cna.seq check
          >D06978B3_B0Cn_3_EGFP-C2_A08_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNCNN NNNNNCNTGG TCCTGCTGGA GTTCGTGNCC GCCGCCGGGA
             61 TCACTCTCGG CATGGANNAG CTGTACAAGT CCGGACTCAG ATCTATGGCA GACACAACCC
            121 CGGAGCCTTG TGGGCAGCTT ATGGTACACT CAGACACTCA CAGTGACACT GTGCTGGCCA
            181 GTCTGGAGGA TCAAAGGAAG AAGGGCTTTC TTTGTGATAT TACTTTAATC GTGGAGAATG
            241 TGCATTTCCG AGCCCATAAA GCCTTGCTGG CCGCCAGTAG CGAGTATTTC TCAATGATGT
            301 TTGCGGAAGA GGGCGAGATT GGCCAGTCTA TCTACATGCT GGAAGGCATG GTTGCAGACA
            361 CCTTTGGCAT CCTGCTGGAA TTTATCTATA CAGGTTATCT ACATGCCAGT GAGAAAACGA
            421 CAGAGCAGAT CCTGGCTACT GCTCAGTTCT TAAAAGTCTA TGACCTGGTA AAGGCATACG
            481 CAGACTTCCA GGATAACCAT AGCGCCCCAA AGCCACCAGC TTTGAACTGC ACTGGGACGC
            541 CAGTGGTGGT TATTTCCAAT AAGAAGAACG ATCCTCTGAA ACGGAAACGG GGAAGACCAA
            601 GAAAAGCCAA TGGTTTGCAG GAAGGGAGAT CAGAACTGGC TGCAGAGGGA GAGCTGCAGC
            661 TGAGAGTGAA CAATTCAGTT CAGAATAGAC AAAACTTTGT GTTTAAAGAA GAAGACAGTG
            721 TCAAGCTGAG TGAGCAGACT CCAGAAGACA AGGAATCAGA GCCTGCTGGT GAGCCAGGAA
            781 GCGTGNAGGA NGTGCCTGCT GAAAAGGACG AGAACTTTGA NNNCNAGGCT GGNGATGGAC
            841 AGGAGANCCN GAGTANGTGC AGCANGCGGA GAATCCGCAN GTCTGTCAAG CTCAAAGANT
            901 ACAAGCTTCT TGGANNNGAA NANGANCNNA NTACTGNCNA GANGNTATGT GGNANAAAAA
            961 AGCGCTCCNN CGGNCCCNAN GNCCGGNGNA AANNNNGNNA NAGANNNTTT NANTATANNN
          1021 NNTTNNNANC NNTNNNNNNN ANNNGCNNNN NNGGGGGNNN NNNNNNN
          //
          Primer: SV40polyA_R2
          Region: SV40 p(A), insert 3'
          Sequence file: RDB18170_B0Cnb.seq check
          >D06978B3_B0Cn_3_SV40polyA_R2_B08_05_ABI24.ab1
              1 NNNNNNNNNN CNGCANNNAA NNNGTTAACN ACNACNATTG CATTCATTTT ATGTTTCAGG
             61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
            181 GAATTCCCAC GATAAAGAAT GGCTGGCTCA CATCTTTAAG GATCTGTAAG ATGATGGCCC
            241 AAAAGGCCAC CCTGCCACTG TTGCTCCAGG GGAAGGGTCT AGTGGAACGG CCAGAGGCGC
            301 TTGGGTCAGC TCTGCTCCTG GCCTAGTGGA GGGGACAGGG GTGGCTCGGG CTCCTGTGTC
            361 AGCTGCAGGT ACTGAGCTGG TTGTTTGCTC CTGGTGGGCG TGGAGATGCT CCAGCGTTTC
            421 CTTGGAAAGT GTGATGACAT GCACTGGCTC AGTCTGGGAG GACTCCATCT GGCCTCCAAT
            481 CACACTGAGG CTCTGAATGT GCTCTGCCTG CTCCTGCTGA GCGGAAAGGA TCAGGCCCTG
            541 CAGCTGCTCA GGCTGCTGTG TGAGCAGGGT GATGTTGGCA GCCGGGTCTG TGGCCATGCT
            601 CTGGGGGCTC TCGGCAGCCA CGATGCTGAC CCCCTGGCTG GGACCTGGCA TGAAGTTAAT
            661 GTTATGCACA GAGTCGGTGA CAAGAAGCTG AATCTCCTGC TCTCCCGACG TGGAGAGCTG
            721 GTATGGTTGC AGCTGAAGAA TGTTCCTGCC CTCGTCCACA TTACTGCCAG AAACACTGCT
            781 GGAGTCTGCC GTGTGCTTCT CTTTGCTGTG GANTTTCNAG TGTGCCTTCN AATTGTCTAG
            841 GCGAGCGAAC TGTAAGCCAC ACTCGGGGCA NGGANAANGG CTTCTTGNCC GTATGCANGA
            901 TGCAGNGTCT CCTCTTGGCN CTGNANTCAN ANAANCATTT GNCACAGANG CTGCNNNANT
            961 ATGGNTTTTC TCCNCNGNGG ATCCTGANGT NGNNNCNGNN NGNNNNNNTT TNNTGNNNNN
          1021 ANTTCCNNNA NANTTCNNNN NNNANNNGNN NNNNNNNN
          //
          Primer: mZbtb24-M_F1
          Region: insert mid
          Sequence file: RDB18170_B0Cnc.seq check
          >D06978B3_B0Cn_3_mZbtb24-M_F1_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGT TTGNNNNNNN NGANNNCNGA ACTGGCTGCA GAGGGAGAGC
             61 TGCAGCTGAG AGTGAACAAT TCAGTTCAGA ATAGACAAAA CTTTGTGTTT AAAGAAGAAG
            121 ACAGTGTCAA GCTGAGTGAG CAGACTCCAG AAGACAAGGA ATCAGAGCCT GCTGGTGAGC
            181 CAGGAAGCGT GGAGGAGGTG CCTGCTGAAA AGGACGAGAA CTTTGACCCC AAGGCTGGGG
            241 ATGGACAGGA GAGCCAGAGT AGGTGCAGCA GGCGGAGAAT CCGCAGGTCT GTCAAGCTCA
            301 AAGACTACAA GCTTCTTGGA GATGAAGATG ACCAGAGTAC TGCCAAGAGG CTATGTGGAA
            361 GAAAAAAGCG CTCCAGCGGT CCCGAGGCCC GGTGTAAAGA CTGCGACAGA GTCTTTAAAT
            421 ATAGTCACTT CTTAGCAATC CACCAGAGAC GCCACACGGG GGAACGACCC TTCAAATGTA
            481 ATGAGTGTGG GAAGGGCTTT GCTCAGAAGC ACTCCTTGCA GGTCCATACC AGGATGCACA
            541 CGGGGGAGCG GCCATACACC TGCACCGTGT GCGGCAAGGC GCTGACCACC AAGCACTCCC
            601 TGCTGGAGCA CATGAGCCTG CACTCAGGAC AGAAGTCTTT TACCTGTGAT CAGTGTGGAA
            661 AATATTTCAG CCAGAAAAGA CAGCTAAAGA GCCATTATCG TGTCCATACA GGCCACTCAC
            721 TACCAGAATG CAGCCACTGC CACCGAAAGT TTATGGACGT GTCCCAGCTG AAGAAGCACC
            781 TGCGGACACA CACAGGCGAG AAGCCATTTA CTTGTGAAAT CTGTGGGAAA TCTTTCACAG
            841 NNAAGAGCTC CCTTCNNACC CACATCNNNA TCCACCGAGG ANAAAAGCCA TACTCCTGCA
            901 GCATCTGNGG CAAATGCTTC TCTGANTCCA GTGCNNAGAN NANANACTGC NTCCNGNNTA
            961 NGGNCAAGAA NNCCCTTCTC CNGNCCCNAN NGNGNNTTNN NNNNCGNNCG NCNNNNNNNT
          1021 TTGNANGGNN NNNTTNAAAN NCNNANNNAA NANNNNNNNN NN
          //
          Primer: mZbtb24-M_R1
          Region: insert mid
          Sequence file: RDB18170_B0Cnd.seq check
          >D06978B3_B0Cn_3_mZbtb24-M_R1_D08_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNANGC TGNNCCCCTG GCTGGGANCT
             61 GGCATGAAGT TAATGTTATG CACAGAGTCG GTGACAAGAA GCTGAATCTC CTGCTCTCCC
            121 GACGTGGAGA GCTGGTATGG TTGCAGCTGA AGAATGTTCC TGCCCTCGTC CACATTACTG
            181 CCAGAAACAC TGCTGGAGTC TGCCGTGTGC TTCTCTTTGC TGTGGATTTT CAAGTGTGCC
            241 TTCAAATTGT CTAGGCGAGC GAACTGTAAG CCACACTCGG GGCAGGAGAA GGGCTTCTTG
            301 CCCGTATGCA GGATGCAGTG TCTCCTCTTG GCACTGGAGT CAGAGAAGCA TTTGCCACAG
            361 ATGCTGCAGG AGTATGGCTT TTCTCCTCGG TGGATCCTGA TGTGGGTCTG AAGGGAGCTC
            421 TTTGCTGTGA AAGATTTCCC ACAGATTTCA CAAGTAAATG GCTTCTCGCC TGTGTGTGTC
            481 CGCAGGTGCT TCTTCAGCTG GGACACGTCC ATAAACTTTC GGTGGCAGTG GCTGCATTCT
            541 GGTAGTGAGT GGCCTGTATG GACACGATAA TGGCTCTTTA GCTGTCTTTT CTGGCTGAAA
            601 TATTTTCCAC ACTGATCACA GGTAAAAGAC TTCTGTCCTG AGTGCAGGCT CATGTGCTCC
            661 AGCAGGGAGT GCTTGGTGGT CAGCGCCTTG CCGCACACGG TGCAGGTGTA TGGCCGCTCC
            721 CCCGTGTGCA TCCTGGTATG GACCTGCAAG GAGTGCTTCT GAGCANAGCC CTTCCCACAC
            781 TCATTACATT TGAAGGGTCG TTCCCCCGTG TGGCGTCTCT GGTGGANTGC TAANAANTGA
            841 CTATATTTAA AGACTCTGTC GCAGTCTTTA CACCGGGCCT CGGGACCGCT GGAGCGCTTT
            901 TTTCNTCCNC ATANCCTCTN GGCAGNANTC TGGTCATCTT CATCTCCNAG AAGCNTGNAN
            961 TCNTTGANCT TGACNNANCT GCGGANTCTC CGCCNGNTGC ANCNANNNTG GNTCTNNNGN
          1021 NCATCCCCNN CNNNGGGGNN NAANNNNNNG TNNTTTTNNN NNNNNNN
          //
          Primer: CMV_Forward
          Region: CMV pro, EGFP 5'
          Sequence file: RDB18170_B0Cne.seq check
          >D06978B3_B0Cn_3_CMV_Foward_E08_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
            121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
            181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
            241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
            301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
            361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
            421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
            481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
            541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
            601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
            661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
            721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
            781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGANCTGTAC AAGTCCGGAC
            841 TCNGATCTAT GGCAGACACN ANCCCCNGAN CCTTGTGGGC AGCTNATGGN ACACTCAGAC
            901 ACTCACAGNG ACACTGTGCT GGCCAGTCTG GANGANNAAG NNAGAANGGC TTTCNTTNGT
            961 GATANTACTT TNNTCGNNGN NANNGNGCAT TTCCNANCCC NNAAAGNNNN NCTGGNCNNC
          1021 NNNANCNNNT NNTTNNNNNN NNANNNTNNN NGNNNNNGGN NNANNNNNGN NNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR_KanR
          Sequence file: RDB18170_B0Cnf.seq check
          >D06978B3_B0Cn_3_SV40pro_ori_F_F08_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNG CCTCNNCCTC TGANCTANTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
            121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
            181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
            241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
            301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
            361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
            421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
            481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
            541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
            601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
            661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
            721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA
            781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GANCGCTTCC
            841 TCGTGCTTTA CGGTATCGCC GCTCNCGATT CGCAGCGCAT CGCCTTCTAT CGCCNTCTTG
            901 NNNANTTCTT CTGANCGGGA NTCTGGGGTT CGAAATGACC GANNAANCGA CGCCNAACCT
            961 GNCNTCNCNA NANTTNNANN NNNNCNNNNN CNTTCTATNN NNGNTGGGNN TNNNNNTCNN
          1021 TTTNNNGNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl