Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

EGFP-ZBTB24 WT (mouse) (#RDB18170)

Expression vector of mouse Zbtb24 wild type.

Clone info. Expression vector of mouse zinc finger and BTB domain containing 24 (Zbtb24) wild type, fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting and fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name mouse Zbtb24 cDNA
Depositor|Developer Unoki, Motoko |
 
Sequence (partial) RDB18170pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nitta, H. et al., J. Hum. Genet. 58 (7), 455-460, 2013 )。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18170 EGFP-ZBTB24 WT (mouse) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The EGFP-ZBTB24 WT (mouse) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18170).

Reference section:

Nitta, H., Unoki, M., Ichiyanagi, K., Kosho, T., Shigemura, T., Takahashi, H., Velasco, G., Francastel, C., Picard, C., Kubota, T., Sasaki, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18170_B0Cnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB18170_B0Cna.seq check
>D06978B3_B0Cn_3_EGFP-C2_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNNNCNTGG TCCTGCTGGA GTTCGTGNCC GCCGCCGGGA
   61 TCACTCTCGG CATGGANNAG CTGTACAAGT CCGGACTCAG ATCTATGGCA GACACAACCC
  121 CGGAGCCTTG TGGGCAGCTT ATGGTACACT CAGACACTCA CAGTGACACT GTGCTGGCCA
  181 GTCTGGAGGA TCAAAGGAAG AAGGGCTTTC TTTGTGATAT TACTTTAATC GTGGAGAATG
  241 TGCATTTCCG AGCCCATAAA GCCTTGCTGG CCGCCAGTAG CGAGTATTTC TCAATGATGT
  301 TTGCGGAAGA GGGCGAGATT GGCCAGTCTA TCTACATGCT GGAAGGCATG GTTGCAGACA
  361 CCTTTGGCAT CCTGCTGGAA TTTATCTATA CAGGTTATCT ACATGCCAGT GAGAAAACGA
  421 CAGAGCAGAT CCTGGCTACT GCTCAGTTCT TAAAAGTCTA TGACCTGGTA AAGGCATACG
  481 CAGACTTCCA GGATAACCAT AGCGCCCCAA AGCCACCAGC TTTGAACTGC ACTGGGACGC
  541 CAGTGGTGGT TATTTCCAAT AAGAAGAACG ATCCTCTGAA ACGGAAACGG GGAAGACCAA
  601 GAAAAGCCAA TGGTTTGCAG GAAGGGAGAT CAGAACTGGC TGCAGAGGGA GAGCTGCAGC
  661 TGAGAGTGAA CAATTCAGTT CAGAATAGAC AAAACTTTGT GTTTAAAGAA GAAGACAGTG
  721 TCAAGCTGAG TGAGCAGACT CCAGAAGACA AGGAATCAGA GCCTGCTGGT GAGCCAGGAA
  781 GCGTGNAGGA NGTGCCTGCT GAAAAGGACG AGAACTTTGA NNNCNAGGCT GGNGATGGAC
  841 AGGAGANCCN GAGTANGTGC AGCANGCGGA GAATCCGCAN GTCTGTCAAG CTCAAAGANT
  901 ACAAGCTTCT TGGANNNGAA NANGANCNNA NTACTGNCNA GANGNTATGT GGNANAAAAA
  961 AGCGCTCCNN CGGNCCCNAN GNCCGGNGNA AANNNNGNNA NAGANNNTTT NANTATANNN
1021 NNTTNNNANC NNTNNNNNNN ANNNGCNNNN NNGGGGGNNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40 p(A), insert 3'
Sequence file: RDB18170_B0Cnb.seq check
>D06978B3_B0Cn_3_SV40polyA_R2_B08_05_ABI24.ab1
    1 NNNNNNNNNN CNGCANNNAA NNNGTTAACN ACNACNATTG CATTCATTTT ATGTTTCAGG
   61 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  121 CTGATTATGA TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACTGCA
  181 GAATTCCCAC GATAAAGAAT GGCTGGCTCA CATCTTTAAG GATCTGTAAG ATGATGGCCC
  241 AAAAGGCCAC CCTGCCACTG TTGCTCCAGG GGAAGGGTCT AGTGGAACGG CCAGAGGCGC
  301 TTGGGTCAGC TCTGCTCCTG GCCTAGTGGA GGGGACAGGG GTGGCTCGGG CTCCTGTGTC
  361 AGCTGCAGGT ACTGAGCTGG TTGTTTGCTC CTGGTGGGCG TGGAGATGCT CCAGCGTTTC
  421 CTTGGAAAGT GTGATGACAT GCACTGGCTC AGTCTGGGAG GACTCCATCT GGCCTCCAAT
  481 CACACTGAGG CTCTGAATGT GCTCTGCCTG CTCCTGCTGA GCGGAAAGGA TCAGGCCCTG
  541 CAGCTGCTCA GGCTGCTGTG TGAGCAGGGT GATGTTGGCA GCCGGGTCTG TGGCCATGCT
  601 CTGGGGGCTC TCGGCAGCCA CGATGCTGAC CCCCTGGCTG GGACCTGGCA TGAAGTTAAT
  661 GTTATGCACA GAGTCGGTGA CAAGAAGCTG AATCTCCTGC TCTCCCGACG TGGAGAGCTG
  721 GTATGGTTGC AGCTGAAGAA TGTTCCTGCC CTCGTCCACA TTACTGCCAG AAACACTGCT
  781 GGAGTCTGCC GTGTGCTTCT CTTTGCTGTG GANTTTCNAG TGTGCCTTCN AATTGTCTAG
  841 GCGAGCGAAC TGTAAGCCAC ACTCGGGGCA NGGANAANGG CTTCTTGNCC GTATGCANGA
  901 TGCAGNGTCT CCTCTTGGCN CTGNANTCAN ANAANCATTT GNCACAGANG CTGCNNNANT
  961 ATGGNTTTTC TCCNCNGNGG ATCCTGANGT NGNNNCNGNN NGNNNNNNTT TNNTGNNNNN
1021 ANTTCCNNNA NANTTCNNNN NNNANNNGNN NNNNNNNN
//
Primer: mZbtb24-M_F1 (Pr0847)
Region: insert mid
Sequence file: RDB18170_B0Cnc.seq check
>D06978B3_B0Cn_3_mZbtb24-M_F1_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGT TTGNNNNNNN NGANNNCNGA ACTGGCTGCA GAGGGAGAGC
   61 TGCAGCTGAG AGTGAACAAT TCAGTTCAGA ATAGACAAAA CTTTGTGTTT AAAGAAGAAG
  121 ACAGTGTCAA GCTGAGTGAG CAGACTCCAG AAGACAAGGA ATCAGAGCCT GCTGGTGAGC
  181 CAGGAAGCGT GGAGGAGGTG CCTGCTGAAA AGGACGAGAA CTTTGACCCC AAGGCTGGGG
  241 ATGGACAGGA GAGCCAGAGT AGGTGCAGCA GGCGGAGAAT CCGCAGGTCT GTCAAGCTCA
  301 AAGACTACAA GCTTCTTGGA GATGAAGATG ACCAGAGTAC TGCCAAGAGG CTATGTGGAA
  361 GAAAAAAGCG CTCCAGCGGT CCCGAGGCCC GGTGTAAAGA CTGCGACAGA GTCTTTAAAT
  421 ATAGTCACTT CTTAGCAATC CACCAGAGAC GCCACACGGG GGAACGACCC TTCAAATGTA
  481 ATGAGTGTGG GAAGGGCTTT GCTCAGAAGC ACTCCTTGCA GGTCCATACC AGGATGCACA
  541 CGGGGGAGCG GCCATACACC TGCACCGTGT GCGGCAAGGC GCTGACCACC AAGCACTCCC
  601 TGCTGGAGCA CATGAGCCTG CACTCAGGAC AGAAGTCTTT TACCTGTGAT CAGTGTGGAA
  661 AATATTTCAG CCAGAAAAGA CAGCTAAAGA GCCATTATCG TGTCCATACA GGCCACTCAC
  721 TACCAGAATG CAGCCACTGC CACCGAAAGT TTATGGACGT GTCCCAGCTG AAGAAGCACC
  781 TGCGGACACA CACAGGCGAG AAGCCATTTA CTTGTGAAAT CTGTGGGAAA TCTTTCACAG
  841 NNAAGAGCTC CCTTCNNACC CACATCNNNA TCCACCGAGG ANAAAAGCCA TACTCCTGCA
  901 GCATCTGNGG CAAATGCTTC TCTGANTCCA GTGCNNAGAN NANANACTGC NTCCNGNNTA
  961 NGGNCAAGAA NNCCCTTCTC CNGNCCCNAN NGNGNNTTNN NNNNCGNNCG NCNNNNNNNT
1021 TTGNANGGNN NNNTTNAAAN NCNNANNNAA NANNNNNNNN NN
//
Primer: mZbtb24-M_R1 (Pr0848)
Region: insert mid
Sequence file: RDB18170_B0Cnd.seq check
>D06978B3_B0Cn_3_mZbtb24-M_R1_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNNANGC TGNNCCCCTG GCTGGGANCT
   61 GGCATGAAGT TAATGTTATG CACAGAGTCG GTGACAAGAA GCTGAATCTC CTGCTCTCCC
  121 GACGTGGAGA GCTGGTATGG TTGCAGCTGA AGAATGTTCC TGCCCTCGTC CACATTACTG
  181 CCAGAAACAC TGCTGGAGTC TGCCGTGTGC TTCTCTTTGC TGTGGATTTT CAAGTGTGCC
  241 TTCAAATTGT CTAGGCGAGC GAACTGTAAG CCACACTCGG GGCAGGAGAA GGGCTTCTTG
  301 CCCGTATGCA GGATGCAGTG TCTCCTCTTG GCACTGGAGT CAGAGAAGCA TTTGCCACAG
  361 ATGCTGCAGG AGTATGGCTT TTCTCCTCGG TGGATCCTGA TGTGGGTCTG AAGGGAGCTC
  421 TTTGCTGTGA AAGATTTCCC ACAGATTTCA CAAGTAAATG GCTTCTCGCC TGTGTGTGTC
  481 CGCAGGTGCT TCTTCAGCTG GGACACGTCC ATAAACTTTC GGTGGCAGTG GCTGCATTCT
  541 GGTAGTGAGT GGCCTGTATG GACACGATAA TGGCTCTTTA GCTGTCTTTT CTGGCTGAAA
  601 TATTTTCCAC ACTGATCACA GGTAAAAGAC TTCTGTCCTG AGTGCAGGCT CATGTGCTCC
  661 AGCAGGGAGT GCTTGGTGGT CAGCGCCTTG CCGCACACGG TGCAGGTGTA TGGCCGCTCC
  721 CCCGTGTGCA TCCTGGTATG GACCTGCAAG GAGTGCTTCT GAGCANAGCC CTTCCCACAC
  781 TCATTACATT TGAAGGGTCG TTCCCCCGTG TGGCGTCTCT GGTGGANTGC TAANAANTGA
  841 CTATATTTAA AGACTCTGTC GCAGTCTTTA CACCGGGCCT CGGGACCGCT GGAGCGCTTT
  901 TTTCNTCCNC ATANCCTCTN GGCAGNANTC TGGTCATCTT CATCTCCNAG AAGCNTGNAN
  961 TCNTTGANCT TGACNNANCT GCGGANTCTC CGCCNGNTGC ANCNANNNTG GNTCTNNNGN
1021 NCATCCCCNN CNNNGGGGNN NAANNNNNNG TNNTTTTNNN NNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB18170_B0Cne.seq check
>D06978B3_B0Cn_3_CMV_Foward_E08_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGANCTGTAC AAGTCCGGAC
  841 TCNGATCTAT GGCAGACACN ANCCCCNGAN CCTTGTGGGC AGCTNATGGN ACACTCAGAC
  901 ACTCACAGNG ACACTGTGCT GGCCAGTCTG GANGANNAAG NNAGAANGGC TTTCNTTNGT
  961 GATANTACTT TNNTCGNNGN NANNGNGCAT TTCCNANCCC NNAAAGNNNN NCTGGNCNNC
1021 NNNANCNNNT NNTTNNNNNN NNANNNTNNN NGNNNNNGGN NNANNNNNGN NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB18170_B0Cnf.seq check
>D06978B3_B0Cn_3_SV40pro_ori_F_F08_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNG CCTCNNCCTC TGANCTANTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
  121 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG
  181 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG
  241 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC
  301 AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC
  361 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
  421 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC
  481 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA
  541 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG
  601 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG
  661 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
  721 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA
  781 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GANCGCTTCC
  841 TCGTGCTTTA CGGTATCGCC GCTCNCGATT CGCAGCGCAT CGCCTTCTAT CGCCNTCTTG
  901 NNNANTTCTT CTGANCGGGA NTCTGGGGTT CGAAATGACC GANNAANCGA CGCCNAACCT
  961 GNCNTCNCNA NANTTNNANN NNNNCNNNNN CNTTCTATNN NNGNTGGGNN TNNNNNTCNN
1021 TTTNNNGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nitta, H., Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients. J. Hum. Genet. 58 (7): 455-460 (2013). PMID 23739126. [link to RRC of NBRP]