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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Mouse Cdca7 promoter P1 (#RDB18169)

Luciferase reporter vector of mouse Cdca7 P1 promoter.

Clone info. Luciferase reporter vector of mouse cell division cycle associated 7 Cdca7 P1 promoter.
Comment Expression was confirmed by the depositor with luciferase assay.
PCR cloning: forward primer (5'- ATA ACG CGT GCG CAT GCT CTC TTC ACA GA -3'), reverse primer (5'- ATA CTC GAG AGC ACT CAG AGC CCG AAC AA -3').
Vector backbone pGL3-basic (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Cdca7 genomic
Depositor|Developer Unoki, Motoko |
 
Sequence (partial) RDB18169pka02.seq provided by the depositor

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18169 Mouse Cdca7 promoter P1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Mouse Cdca7 promoter P1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18169).

Reference section:

Aktar, S., Sasaki, H., Unoki, M., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18169_B0Cnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site, insert 5'
Sequence file: RDB18169_B0Cna.seq check
>D06978B2_B0Cn_1_pGL4ins_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNACNAN NCNNNATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA
   61 CATTTCTCTA TCGATAGGTA CCGAGCTCTT ACGCGTGCGC ATGCTCTCTT CACAGAAAAG
  121 GACTGTGGCA ACATCAATAC TAAGAGAAAA GTTTTGAAAT AAGGGGGAAT GCTTACACTT
  181 CAAGAAATGT TTTAAATCTA TGATTATAAT TAAAGCTCTT TTAAAAAGCA CCCAAGGTTT
  241 TTCGGTCGTT GCTGTTTGGG TTTTTTTTTT TCCCCCTGTG TTTTACTCTT TAAAAACAGG
  301 GGAGCCTCGT TTCATCCCTG TGCCGGTCTT AGTGAGAAGG GCTATAAGAA AGAAAGAAAG
  361 AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGATCTTCTA
  421 ACTAGCTCTG GTTGTTCCCA ATTTTGCCTG GAGAAAAATG GACAATCCAC AGTGTAATCG
  481 CCTGCAGCTT TTATCTCCGA AAGATAAAGC TCTAAAGACT TTTACCCTTC GGAGAGGCTT
  541 TACTCAACTG AAGGGCTATT TTTAAACCCA CGCCGGAATC TTTCTTGAAG CAACAGGACT
  601 TAACTGGTCT ATTGACTACA TATAGATAGT GGTGTGGTTG CTAGATAAGA GGTGCTATGC
  661 TCTCAGATAT TTGTGGGTGG TATCCCCGTT CTGTTCTTTA ACATTTTTAA GAGATTAAAC
  721 TACAGCGAAA GTGTGAACGC AGTAAGAGGC TCTACCAGCC TTACAATCTC CCGTTTTGTA
  781 TGCAAATCAG ACGGCCAGTC TCAGGACCTC GGCAGCCACT TTCGCGCCNC TTCNCAGGAN
  841 CGCGCAGCTT CATAAAAAGC CAGNGGCACT GANTTCCCNC AACGCCNCNG NTTCATAGAA
  901 CATTCANCAN TTTCCNANGA NNNTCANGGN TATTACAACC NCNANAANCG CTTCTATTTT
  961 TTTCCNNANC NNGGNNNCTT CGGGNNNNNN NAAANANNNC ATNNNANCTN GNNNGNNNCN
1021 NCNNNCCNGN NNNNNNAAAN NCNNNNNNGN NNNNNNNNN
//
Primer: Luc_R (Pr0146)
Region: Luc 5', insert 3'
Sequence file: RDB18169_B0Cnb.seq check
>D06978B2_B0Cn_1_Luc_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN GNNNNNNGNN NGNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
   61 TCCNTCTTCC NGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
  121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CTCGAGAGCA
  181 CTCAGAGCCC GAACAACCCG AGCTCCGGGA CAGAGGCGGG GCGCGCACTC CGCCGCCGGA
  241 GGGGCGGGGC CACCGGCTTT CCCGCGCCGG GACGCAGGTC CTGCCAGCTA AGATGACCTC
  301 TTTAGCGGTA CCCGAAGCGT CCCAGCTCTG GAAAAAAATA GAAGCGGTTC TCGTGGTTGT
  361 AATAACCTTG AGTGTCCTCG GAAACTGCTG AATGTTCTAT GAAACAGTGG CGTTGTGGGA
  421 ACTCAGTGCC ACTGGCTTTT TATGAAGCTG CGCGGTCCTG GGAAGTGGCG CGAAAGTGGC
  481 TGCCGAGGTC CTGAGACTGG CCGTCTGATT TGCATACAAA ACGGGAGATT GTAAGGCTGG
  541 TAGAGCCTCT TACTGCGTTC ACACTTTCGC TGTAGTTTAA TCTCTTAAAA ATGTTAAAGA
  601 ACAGAACGGG GATACCACCC ACAAATATCT GAGAGCATAG CACCTCTTAT CTAGCAACCA
  661 CACCACTATC TATATGTAGT CAATAGACCA GTTAAGTCCT GTTGCTTCAA GAAAGATTCC
  721 GGCGTGGGTT TAAAAATAGC CCTTCAGTTG AGTAAAGCCT CTCCGAAGGN TAAAAGTCTT
  781 TAGAGCTTTA TCTTTCGGAG ATAAAAGCTG CAGGCGATTA CACTGTGGAT TGTCCATTTT
  841 TCTCCNGGCA AAATTGGGAA CNACCAGAGC TAGTTAGAAG ANCTTTCTTT CTTTCTTNCT
  901 TTCNTTCTTN CNTNNNTNCN TNCNTNCNTT CTTTCNTTCT TNCTTTCNNA NAGCCCTTCT
  961 CACNAAGACC GGNNCANGNN NGAANNGANG NTNCCCNNTT TTTNANNAGT NAAACNCNNN
1021 GGGNAAAAAA AAANNNNNNN NNN
//
Primer: pAxCALNLF1 (Pr0166)
Region: Luc 3'
Sequence file: RDB18169_B0Cnc.seq check
>D06978B2_B0Cn_1_pAxCALNLF1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTG TCTNNTNNNN NNGGCCGGCC GCCCCGACTC TAGAATTACA
   61 CGGCGATCTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC TCTGATTTTT CTTGCGTCGA
  121 GTTTTCCGGT AAGACCTTTC GGTACTTCGT CCACAAACAC AACTCCTCCG CGCAACTTTT
  181 TCGCGGTTGT TACTTGACTG GCGACGTAAT CCACGATCTC TTTTTCCGTC ATCGTCTTTC
  241 CGTGCTCCAA AACAACAACG GCGGCGGGAA GTTCACCGGC GTCATCGTCG GGAAGACCTG
  301 CGACACCTGC GTCGAAGATG TTGGGGTGTT GGAGCAAGAT GGATTCCAAT TCAGCGGGAG
  361 CCACCTGATA GCCTTTGTAC TTAATCAGAG ACTTCAGGCG GTCAACGATG AAGAAGTGTT
  421 CGTCTTCGTC CCAGTAAGCT ATGTCTCCAG AATGTAGCCA TCCATCCTTG TCAATCAAGG
  481 CGTTGGTCGC TTCCGGATTG TTTACATAAC CGGACATAAT CATAGGACCT CTCACACACA
  541 GTTCGCCTCT TTGATTAACG CCCAGCGTTT TCCCGGTATC CAGATCCACA ACCTTCGCTT
  601 CAAAAAATGG AACAACTTTA CCGACCGCGC CCGGTTTATC ATCCCCCTCG GGTGTAATCA
  661 GAATAGCTGA TGTAGTCTCA GTGAGCCCAT ATCCTTGCCT GATACCTGGC AGATGGAACC
  721 TCTTGGCAAC CGCTTCCCCG ACTTCCTTAG AGAGGGGAGC GCCACCAGAA GCAATTTCGT
  781 GTAAATTAGA TAAATCGTAT TTGTCNATCA NAGTGCTTTT GGCGAANAAN GAGAATAGGG
  841 TTGGCACCAG CAGCGCACTT TGAATCTTGT AATCCTGAAN GCTCCTCANA AACAGCTCTT
  901 CTNCAANTCT ATACNNNAAG ANNANTCNAA ATCCNCATAT CAAATATCCN AGNGNANNNN
  961 NCNTTCCNAA NCNGNGATGG NNNGGNNCNN CNNTTAAAAT CGCAGTATNN NGNAATGNNT
1021 TGANNGNNNA ANANNNGNAT NNNNGNNNNG NNANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP]