Luciferase reporter vector of mouse Cdca7 P1 promoter.
Clone info. | Luciferase reporter vector of mouse cell division cycle associated 7 Cdca7 P1 promoter. |
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Comment | Expression was confirmed by the depositor with luciferase assay. PCR cloning: forward primer (5'- ATA ACG CGT GCG CAT GCT CTC TTC ACA GA -3'), reverse primer (5'- ATA CTC GAG AGC ACT CAG AGC CCG AAC AA -3'). |
Vector backbone | pGL3-basic (plasmid) |
Size of vector backbone | 4.8 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Cdca7 genomic |
Depositor|Developer | Unoki, Motoko | |
Sequence (partial) | RDB18169pka02.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18169 | Mouse Cdca7 promoter P1 | DNA solution |
Materials & Methods section:
The Mouse Cdca7 promoter P1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18169). |
Reference section:
Aktar, S., Sasaki, H., Unoki, M., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18169_B0Cnp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGL4ins_F (Pr0665) Region: pause site, insert 5' Sequence file: RDB18169_B0Cna.seq |
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>D06978B2_B0Cn_1_pGL4ins_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNACNAN NCNNNATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA 61 CATTTCTCTA TCGATAGGTA CCGAGCTCTT ACGCGTGCGC ATGCTCTCTT CACAGAAAAG 121 GACTGTGGCA ACATCAATAC TAAGAGAAAA GTTTTGAAAT AAGGGGGAAT GCTTACACTT 181 CAAGAAATGT TTTAAATCTA TGATTATAAT TAAAGCTCTT TTAAAAAGCA CCCAAGGTTT 241 TTCGGTCGTT GCTGTTTGGG TTTTTTTTTT TCCCCCTGTG TTTTACTCTT TAAAAACAGG 301 GGAGCCTCGT TTCATCCCTG TGCCGGTCTT AGTGAGAAGG GCTATAAGAA AGAAAGAAAG 361 AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGATCTTCTA 421 ACTAGCTCTG GTTGTTCCCA ATTTTGCCTG GAGAAAAATG GACAATCCAC AGTGTAATCG 481 CCTGCAGCTT TTATCTCCGA AAGATAAAGC TCTAAAGACT TTTACCCTTC GGAGAGGCTT 541 TACTCAACTG AAGGGCTATT TTTAAACCCA CGCCGGAATC TTTCTTGAAG CAACAGGACT 601 TAACTGGTCT ATTGACTACA TATAGATAGT GGTGTGGTTG CTAGATAAGA GGTGCTATGC 661 TCTCAGATAT TTGTGGGTGG TATCCCCGTT CTGTTCTTTA ACATTTTTAA GAGATTAAAC 721 TACAGCGAAA GTGTGAACGC AGTAAGAGGC TCTACCAGCC TTACAATCTC CCGTTTTGTA 781 TGCAAATCAG ACGGCCAGTC TCAGGACCTC GGCAGCCACT TTCGCGCCNC TTCNCAGGAN 841 CGCGCAGCTT CATAAAAAGC CAGNGGCACT GANTTCCCNC AACGCCNCNG NTTCATAGAA 901 CATTCANCAN TTTCCNANGA NNNTCANGGN TATTACAACC NCNANAANCG CTTCTATTTT 961 TTTCCNNANC NNGGNNNCTT CGGGNNNNNN NAAANANNNC ATNNNANCTN GNNNGNNNCN 1021 NCNNNCCNGN NNNNNNAAAN NCNNNNNNGN NNNNNNNNN // |
Primer: Luc_R (Pr0146) Region: Luc 5', insert 3' Sequence file: RDB18169_B0Cnb.seq |
>D06978B2_B0Cn_1_Luc_R_B01_02_ABI08.ab1 1 NNNNNNNNNN GNNNNNNGNN NGNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT 61 TCCNTCTTCC NGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT 121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CTCGAGAGCA 181 CTCAGAGCCC GAACAACCCG AGCTCCGGGA CAGAGGCGGG GCGCGCACTC CGCCGCCGGA 241 GGGGCGGGGC CACCGGCTTT CCCGCGCCGG GACGCAGGTC CTGCCAGCTA AGATGACCTC 301 TTTAGCGGTA CCCGAAGCGT CCCAGCTCTG GAAAAAAATA GAAGCGGTTC TCGTGGTTGT 361 AATAACCTTG AGTGTCCTCG GAAACTGCTG AATGTTCTAT GAAACAGTGG CGTTGTGGGA 421 ACTCAGTGCC ACTGGCTTTT TATGAAGCTG CGCGGTCCTG GGAAGTGGCG CGAAAGTGGC 481 TGCCGAGGTC CTGAGACTGG CCGTCTGATT TGCATACAAA ACGGGAGATT GTAAGGCTGG 541 TAGAGCCTCT TACTGCGTTC ACACTTTCGC TGTAGTTTAA TCTCTTAAAA ATGTTAAAGA 601 ACAGAACGGG GATACCACCC ACAAATATCT GAGAGCATAG CACCTCTTAT CTAGCAACCA 661 CACCACTATC TATATGTAGT CAATAGACCA GTTAAGTCCT GTTGCTTCAA GAAAGATTCC 721 GGCGTGGGTT TAAAAATAGC CCTTCAGTTG AGTAAAGCCT CTCCGAAGGN TAAAAGTCTT 781 TAGAGCTTTA TCTTTCGGAG ATAAAAGCTG CAGGCGATTA CACTGTGGAT TGTCCATTTT 841 TCTCCNGGCA AAATTGGGAA CNACCAGAGC TAGTTAGAAG ANCTTTCTTT CTTTCTTNCT 901 TTCNTTCTTN CNTNNNTNCN TNCNTNCNTT CTTTCNTTCT TNCTTTCNNA NAGCCCTTCT 961 CACNAAGACC GGNNCANGNN NGAANNGANG NTNCCCNNTT TTTNANNAGT NAAACNCNNN 1021 GGGNAAAAAA AAANNNNNNN NNN // |
Primer: pAxCALNLF1 (Pr0166) Region: Luc 3' Sequence file: RDB18169_B0Cnc.seq |
>D06978B2_B0Cn_1_pAxCALNLF1_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNTG TCTNNTNNNN NNGGCCGGCC GCCCCGACTC TAGAATTACA 61 CGGCGATCTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC TCTGATTTTT CTTGCGTCGA 121 GTTTTCCGGT AAGACCTTTC GGTACTTCGT CCACAAACAC AACTCCTCCG CGCAACTTTT 181 TCGCGGTTGT TACTTGACTG GCGACGTAAT CCACGATCTC TTTTTCCGTC ATCGTCTTTC 241 CGTGCTCCAA AACAACAACG GCGGCGGGAA GTTCACCGGC GTCATCGTCG GGAAGACCTG 301 CGACACCTGC GTCGAAGATG TTGGGGTGTT GGAGCAAGAT GGATTCCAAT TCAGCGGGAG 361 CCACCTGATA GCCTTTGTAC TTAATCAGAG ACTTCAGGCG GTCAACGATG AAGAAGTGTT 421 CGTCTTCGTC CCAGTAAGCT ATGTCTCCAG AATGTAGCCA TCCATCCTTG TCAATCAAGG 481 CGTTGGTCGC TTCCGGATTG TTTACATAAC CGGACATAAT CATAGGACCT CTCACACACA 541 GTTCGCCTCT TTGATTAACG CCCAGCGTTT TCCCGGTATC CAGATCCACA ACCTTCGCTT 601 CAAAAAATGG AACAACTTTA CCGACCGCGC CCGGTTTATC ATCCCCCTCG GGTGTAATCA 661 GAATAGCTGA TGTAGTCTCA GTGAGCCCAT ATCCTTGCCT GATACCTGGC AGATGGAACC 721 TCTTGGCAAC CGCTTCCCCG ACTTCCTTAG AGAGGGGAGC GCCACCAGAA GCAATTTCGT 781 GTAAATTAGA TAAATCGTAT TTGTCNATCA NAGTGCTTTT GGCGAANAAN GAGAATAGGG 841 TTGGCACCAG CAGCGCACTT TGAATCTTGT AATCCTGAAN GCTCCTCANA AACAGCTCTT 901 CTNCAANTCT ATACNNNAAG ANNANTCNAA ATCCNCATAT CAAATATCCN AGNGNANNNN 961 NCNTTCCNAA NCNGNGATGG NNNGGNNCNN CNNTTAAAAT CGCAGTATNN NGNAATGNNT 1021 TGANNGNNNA ANANNNGNAT NNNNGNNNNG NNANNNNNNN NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
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