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Mouse Cdca7 promoter P1

Luciferase reporter vector of mouse Cdca7 P1 promoter.

Catalog number RDB18169
Resource name Mouse Cdca7 promoter P1
Clone info. Luciferase reporter vector of mouse cell division cycle associated 7 Cdca7 P1 promoter.
Comment Expression was confirmed by the depositor with luciferase assay.
PCR cloning: forward primer (5'- ATA ACG CGT GCG CAT GCT CTC TTC ACA GA -3'), reverse primer (5'- ATA CTC GAG AGC ACT CAG AGC CCG AAC AA -3').
Vector backbone pGL3-basic (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Cdca7 genomic
Depositor|Developer Unoki, Motoko |
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External Database
mouse Cdca7

          Reference sequence
            
           
          Sequence (partial) RDB18169pka02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB18169 Mouse Cdca7 promoter P1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18169_B0Cnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site, insert 5'
          Sequence file: RDB18169_B0Cna.seq check
          >D06978B2_B0Cn_1_pGL4ins_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNACNAN NCNNNATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA
             61 CATTTCTCTA TCGATAGGTA CCGAGCTCTT ACGCGTGCGC ATGCTCTCTT CACAGAAAAG
            121 GACTGTGGCA ACATCAATAC TAAGAGAAAA GTTTTGAAAT AAGGGGGAAT GCTTACACTT
            181 CAAGAAATGT TTTAAATCTA TGATTATAAT TAAAGCTCTT TTAAAAAGCA CCCAAGGTTT
            241 TTCGGTCGTT GCTGTTTGGG TTTTTTTTTT TCCCCCTGTG TTTTACTCTT TAAAAACAGG
            301 GGAGCCTCGT TTCATCCCTG TGCCGGTCTT AGTGAGAAGG GCTATAAGAA AGAAAGAAAG
            361 AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGAAAGAAAG AAAGAAAGAA AGATCTTCTA
            421 ACTAGCTCTG GTTGTTCCCA ATTTTGCCTG GAGAAAAATG GACAATCCAC AGTGTAATCG
            481 CCTGCAGCTT TTATCTCCGA AAGATAAAGC TCTAAAGACT TTTACCCTTC GGAGAGGCTT
            541 TACTCAACTG AAGGGCTATT TTTAAACCCA CGCCGGAATC TTTCTTGAAG CAACAGGACT
            601 TAACTGGTCT ATTGACTACA TATAGATAGT GGTGTGGTTG CTAGATAAGA GGTGCTATGC
            661 TCTCAGATAT TTGTGGGTGG TATCCCCGTT CTGTTCTTTA ACATTTTTAA GAGATTAAAC
            721 TACAGCGAAA GTGTGAACGC AGTAAGAGGC TCTACCAGCC TTACAATCTC CCGTTTTGTA
            781 TGCAAATCAG ACGGCCAGTC TCAGGACCTC GGCAGCCACT TTCGCGCCNC TTCNCAGGAN
            841 CGCGCAGCTT CATAAAAAGC CAGNGGCACT GANTTCCCNC AACGCCNCNG NTTCATAGAA
            901 CATTCANCAN TTTCCNANGA NNNTCANGGN TATTACAACC NCNANAANCG CTTCTATTTT
            961 TTTCCNNANC NNGGNNNCTT CGGGNNNNNN NAAANANNNC ATNNNANCTN GNNNGNNNCN
          1021 NCNNNCCNGN NNNNNNAAAN NCNNNNNNGN NNNNNNNNN
          //
          Primer: Luc_R
          Region: Luc 5', insert 3'
          Sequence file: RDB18169_B0Cnb.seq check
          >D06978B2_B0Cn_1_Luc_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN GNNNNNNGNN NGNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
             61 TCCNTCTTCC NGCGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
            121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CTCGAGAGCA
            181 CTCAGAGCCC GAACAACCCG AGCTCCGGGA CAGAGGCGGG GCGCGCACTC CGCCGCCGGA
            241 GGGGCGGGGC CACCGGCTTT CCCGCGCCGG GACGCAGGTC CTGCCAGCTA AGATGACCTC
            301 TTTAGCGGTA CCCGAAGCGT CCCAGCTCTG GAAAAAAATA GAAGCGGTTC TCGTGGTTGT
            361 AATAACCTTG AGTGTCCTCG GAAACTGCTG AATGTTCTAT GAAACAGTGG CGTTGTGGGA
            421 ACTCAGTGCC ACTGGCTTTT TATGAAGCTG CGCGGTCCTG GGAAGTGGCG CGAAAGTGGC
            481 TGCCGAGGTC CTGAGACTGG CCGTCTGATT TGCATACAAA ACGGGAGATT GTAAGGCTGG
            541 TAGAGCCTCT TACTGCGTTC ACACTTTCGC TGTAGTTTAA TCTCTTAAAA ATGTTAAAGA
            601 ACAGAACGGG GATACCACCC ACAAATATCT GAGAGCATAG CACCTCTTAT CTAGCAACCA
            661 CACCACTATC TATATGTAGT CAATAGACCA GTTAAGTCCT GTTGCTTCAA GAAAGATTCC
            721 GGCGTGGGTT TAAAAATAGC CCTTCAGTTG AGTAAAGCCT CTCCGAAGGN TAAAAGTCTT
            781 TAGAGCTTTA TCTTTCGGAG ATAAAAGCTG CAGGCGATTA CACTGTGGAT TGTCCATTTT
            841 TCTCCNGGCA AAATTGGGAA CNACCAGAGC TAGTTAGAAG ANCTTTCTTT CTTTCTTNCT
            901 TTCNTTCTTN CNTNNNTNCN TNCNTNCNTT CTTTCNTTCT TNCTTTCNNA NAGCCCTTCT
            961 CACNAAGACC GGNNCANGNN NGAANNGANG NTNCCCNNTT TTTNANNAGT NAAACNCNNN
          1021 GGGNAAAAAA AAANNNNNNN NNN
          //
          Primer: pAxCALNLF1
          Region: Luc 3'
          Sequence file: RDB18169_B0Cnc.seq check
          >D06978B2_B0Cn_1_pAxCALNLF1_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNTG TCTNNTNNNN NNGGCCGGCC GCCCCGACTC TAGAATTACA
             61 CGGCGATCTT TCCGCCCTTC TTGGCCTTTA TGAGGATCTC TCTGATTTTT CTTGCGTCGA
            121 GTTTTCCGGT AAGACCTTTC GGTACTTCGT CCACAAACAC AACTCCTCCG CGCAACTTTT
            181 TCGCGGTTGT TACTTGACTG GCGACGTAAT CCACGATCTC TTTTTCCGTC ATCGTCTTTC
            241 CGTGCTCCAA AACAACAACG GCGGCGGGAA GTTCACCGGC GTCATCGTCG GGAAGACCTG
            301 CGACACCTGC GTCGAAGATG TTGGGGTGTT GGAGCAAGAT GGATTCCAAT TCAGCGGGAG
            361 CCACCTGATA GCCTTTGTAC TTAATCAGAG ACTTCAGGCG GTCAACGATG AAGAAGTGTT
            421 CGTCTTCGTC CCAGTAAGCT ATGTCTCCAG AATGTAGCCA TCCATCCTTG TCAATCAAGG
            481 CGTTGGTCGC TTCCGGATTG TTTACATAAC CGGACATAAT CATAGGACCT CTCACACACA
            541 GTTCGCCTCT TTGATTAACG CCCAGCGTTT TCCCGGTATC CAGATCCACA ACCTTCGCTT
            601 CAAAAAATGG AACAACTTTA CCGACCGCGC CCGGTTTATC ATCCCCCTCG GGTGTAATCA
            661 GAATAGCTGA TGTAGTCTCA GTGAGCCCAT ATCCTTGCCT GATACCTGGC AGATGGAACC
            721 TCTTGGCAAC CGCTTCCCCG ACTTCCTTAG AGAGGGGAGC GCCACCAGAA GCAATTTCGT
            781 GTAAATTAGA TAAATCGTAT TTGTCNATCA NAGTGCTTTT GGCGAANAAN GAGAATAGGG
            841 TTGGCACCAG CAGCGCACTT TGAATCTTGT AATCCTGAAN GCTCCTCANA AACAGCTCTT
            901 CTNCAANTCT ATACNNNAAG ANNANTCNAA ATCCNCATAT CAAATATCCN AGNGNANNNN
            961 NCNTTCCNAA NCNGNGATGG NNNGGNNCNN CNNTTAAAAT CGCAGTATNN NGNAATGNNT
          1021 TGANNGNNNA ANANNNGNAT NNNNGNNNNG NNANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl