Luciferase reporter vector of human CDCA7 P4M promoter [P4 promoter (-81 to +119) with deletion (-220 to -214)].
Clone info. | Luciferase reporter vector of human cell division cycle associated 7 CDCA7 P4M promoter [P4 promoter (-81 to +119) with deletion (-220 to -214)]. |
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Comment | Expression was confirmed by the depositor with luciferase assay. Mutagenesis: forward primer (5'- GTG TCC CAA CGC GGG AAA G -3'), reverse primer (5'- GCA AGC TGT GGT AAC GGC -3'). The positions of the transcription start sites were determined using UCSC Genome Browser GRCh38/hg38. |
Vector backbone | pGL3-basic (plasmid) |
Size of vector backbone | 4.8 kb |
Selectable markers | Ampicillin |
Gene/insert name | human CDCA7 genomic |
Depositor|Developer | Unoki, Motoko | |
Sequence (partial) | RDB18164pka01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019) Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18164 | Human CDCA7 promoter P4M | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The Human CDCA7 promoter P4M was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18164). |
Reference section:
Aktar, S., Sasaki, H., Unoki, M., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277. [link to RRC of NBRP] |
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