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Human CDCA7 promoter P2

Luciferase reporter vector of human CDCA7 P2 promoter -452 to +119.

Catalog number RDB18160
Resource name Human CDCA7 promoter P2
Clone info. Luciferase reporter vector of human cell division cycle associated 7 CDCA7 P2 promoter -452 to +119.
Comment Expression was confirmed by the depositor with luciferase assay.
PCR cloning: forward primer (5'- ATA ACG CGT CGG TGA ACT CGA TTA TGA AG -3'), reverse primer (5'- ATA AGA TCT TGC CCA GAT CGG CGC TTG GA -3'). The positions of the transcription start sites were determined using UCSC Genome Browser GRCh38/hg38.
Vector backbone pGL3-basic (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name human CDCA7 genomic
Depositor|Developer Unoki, Motoko |
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External Database
human CDCA7
          Reference sequence
            
           
          Sequence (partial) RDB18160pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aktar, S. et al., Genes Cells. 24 (11), 746-755, 2019)。
          付加的提供条件：
          本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18160 Human CDCA7 promoter P2 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Aktar, S., Identification of ZBTB24 protein domains and motifs for heterochromatin localization and transcriptional activation. Genes Cells 24 (11): 746-755 (2019). PMID 31561277.

          2022.10.11

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