Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFLAG-mRoquin-1-WT

Expression vector of mouse Roquin-1 (Rc3h1).

Sequence (full)	RDB18151zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18151_B1Alp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, FLAG, insert 5' Sequence file: RDB18151_B1Alf.seq
>D06974A4_B1Al_3_CMV_Forward_D02_11_ABI24.ab1     1 NNNNNNNNNN NNCCNTNNNN NCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG   121 ACAAGCTTGC GGCCGCGCCT GTACAAGCTC CACAATGGAC GGATTTCCTT TCCTGCCCAA   181 TTTGCACTCA GACTTTCGAC GAAACAATTC GAAAGCCCAT CAGTTTGGGC TGTGGCCATA   241 CTGTCTGCAA AATGTGCCTG AATAAACTCC ACCGTAAGGC TTGCCCATTT GACCAGACCA   301 CTATCAATAC GGACATTGAG CTTCTTCCTG TGAACTCAGC ATTACTGCAG CTAGTGGGTG   361 CTCAGATCCC TGAGCAACAG CCCATTACTT TGTGTAGTGG GGTTGAAGAT ACAAAGCATT   421 ATGAGGAAGC CAAGAAATGT GTAGAAGAAT TAGCATTGTA CCTGAAGCCA CTCAGCAGTG   481 CGAGAGGTGT GGGTCTAAAT AGCACTACTC AGAGTGTTCT GAGTCGCCCA ATGCAGAGGA   541 AACTTGTGAC TCTGGTTCAT TGCCAGCTAG TGGAAGAAGA AGGCAGGATC CGTGCTATGA   601 GGGCTGCTCG ATCTTTAGGT GAACGCACAG TTACAGAGCT CATTCTCCAG CACCAGAATC   661 CTCAGCAACT CTCTTCTAAT CTCTGGGCAG CAGTCAGAGC TAGGGGCTGC CAGTTCCTTG   721 GACCAGCGAT GCAGGAGGAA GCTCTGAAGC TGGTCTTGCT GGCCTTAGAA GATGGTTCTG   781 CATTGTCTCG GAAAGTGTTG GTTCTCTTCG TGGTGCAAAG ACTGGANCCA CGTTTTCCTC   841 AAGCCTCTAA AACCAGCATT GNGCATGTTG NTCAGCTCCT CTACAGANCC TCCTGCTNCN   901 AGGTCANNNA ACGTGATGAA NANTCTTCTT TGATGCANCT GAAANAANAA TTTANNNNTT   961 ACNANNCTCT GANGANANAG CATGANNNCN NNNNANNGNN AANNGNTANN GNANNCTNGN 1021 NNNNNNNNN //
Primer: hGHpolyA-R (Pr0350) Region: hGH p(A), insert 3' Sequence file: RDB18151_B1Alg.seq
>D06974A4_B1Al_3_hGHpolyA-R_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN AANANTATAG AAGGNNACCT AGTCAGACAA AATGATGCAA    61 CTTAATTTTA TTAGGACAAG GCTGGTGGGC ACTGGAGTGG CAACTTCCAG GGCCAGGAGA   121 GGCACTGGGG AGGGGTCACA GGGATGCCAC CCGGGATCCT CTAGAGTCGA CTGGTACCGA   181 TATCAGATCT ATCGATGAAT TCTTAGGGAG CAGAATTGGA AACAACTCCT GATCTCTGGG   241 AATCATTGTT ATCTCCTCCT CCCTCAGAGT CTTCTGACAG ATTCAAAGAG CTGGTCTTGT   301 TTGATAGGAG GCTGAGATTC TCTTGTGTCA AGGCTGATCC ATTTGGTACA TCACTGAATG   361 TCAATGTAAG GTCCTCAGTG CGGATCTTGT TTTGGGGCTC GGGCTGGCCA TTTTCTGCTT   421 GCTTGCTTGT ACCCAGTTTG CTTTTCATGT CTACAGAACA CTGGTTCTCC ATACTCAATT   481 CCCGGGTCCT CTTCCCAATT TCTCTTTCCA CCTGATGCAG TTCCAAGCTG AGCTGCTCAC   541 TTGAGATCAT TCCAGGCCAT TTTGGAGGCA GCTGAGGGGG CTGTGGCTGG CTTGCCAGGG   601 TGTTCACCTC CCTCTGTAAC ACCAGCCGGT TACTAACAGC CTCTAGTCCT CGTAGCTGGG   661 TCTGCTGGCT GATCTGATGG TTCAGCTGCT GCAATTCTAG CCGTAATTGT TCTCTTTCAG   721 CAGATAGAAN GGGTTTTCCA TGACTGGCCA CTTCTGCCAT GGACATTTTC TCCCTCTCAA   781 TGAAGTGTCC CTGANGANGT ATGTTTGCAT GANGTGAGTA TGGAGAATCT CCCCAGCCAC   841 CATGAGCTCC ATATGCACTG TAATCGGAAN TGTTAATAGA TTTTGTGGGT GCTCCCTGAN   901 GTGCTATAGC TTGCAGTGGG CCANCACCCT GATACAGTGT TTTNNANGNT CNANANANGG   961 NNCNNNNTCN AGANNNTGNT NNNCNGNCTN CNAACNGNNN GNNNNCNTCN TCNNNCGNNN 1021 CCNNNGNNNN NCNNNNNNNN NN //
Primer: mRoquin-M_F1 (Pr0856) Region: insert mid Sequence file: RDB18151_B1Alh.seq
>D06974A4_B1Al_3_mRoquin-M_F1_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG NNNNTCTGGT TNNTTGCCNG CTAGTGGAAG AAGAAGGNAG    61 GATCCGTGCT ATGAGGGCTG CTCGATCTTT AGGTGAACGC ACAGTTACAG AGCTCATTCT   121 CCAGCACCAG AATCCTCAGC AACTCTCTTC TAATCTCTGG GCAGCAGTCA GAGCTAGGGG   181 CTGCCAGTTC CTTGGACCAG CGATGCAGGA GGAAGCTCTG AAGCTGGTCT TGCTGGCCTT   241 AGAAGATGGT TCTGCATTGT CTCGGAAAGT GTTGGTTCTC TTCGTGGTGC AAAGACTGGA   301 GCCACGTTTT CCTCAAGCCT CTAAAACCAG CATTGGGCAT GTTGTTCAGC TCCTCTACAG   361 AGCCTCCTGC TTCAAGGTCA CCAAACGTGA TGAAGACTCT TCTTTGATGC AACTGAAAGA   421 AGAATTTAGG ACTTACGAAG CTCTGAGGAG AGAGCATGAC TCCCAGATAG TGCAAATTGC   481 TATGGAAGCT GGCTTAAGGA TTGCACCAGA CCAGTGGTCC TCTTTGCTTT ATGGGGATCA   541 GTCTCACAAA TCTCATATGC AGTCTATTAT TGACAAGTTA CAGACCCCTG CCTCATTTGC   601 ACAGAGTGTT CAGGAGCTAA CAATTGCTCT CCAGCGGACT GGAGACCCAG CGAATTTGAA   661 CCGACTAAGA CCCCATTTGG AGTTACTCGC AAACATTGAC CCTAGTCCAG ATGCTCCTCC   721 TCCAACATGG GAACAGCTAG AAAATGGGCT GGTTGCTGTA CGTACGGTAG TGCATGGATT   781 GGTTGANTAT ATCCAGAACC ACAGCAAAAA AGGAGCAGAT CAACNACAGC CTCCACAGCA   841 TANCAAATAC AAAACATACA TGTGTCGAGA TANGAAGCAA ANGGNANGAN GNCCTCNNGG   901 TGNCAGCTGT ACGTTTGCAC ATTCNCANGA ANAANTNNAA AANTTTCGNA AAANGNANNA   961 ACGTCTGGNT CCNNNANNAN CNTTGNNNGN NNCNTNNGGN CNNCTTNNTG NNNNGGNNCN 1021 GNCNNNNNNN NNN //
Primer: mRoquin-M_R1 (Pr0857) Region: insert mid Sequence file: RDB18151_B1Ali.seq
>D06974A4_B1Al_3_mRoquin-M_R1_G02_20_ABI24.ab1     1 NNNNNNNNNN NNCNNCNAGT CNTCGTAGCT GGGTCTGCTG GCTGATCTGA TGGTTCAGCT    61 GCTGCAATTC TAGCCGTAAT TGTTCTCTTT CAGCAGATAG AAGGGGTTTT CCATGACTGG   121 CCACTTCTGC CATGGACATT TTCTCCCTCT CAATGAAGTG TCCCTGAGGA GGTATGTTTG   181 CATGAGGTGA GTATGGAGAA TCTCCCCAGC CACCATGAGC TCCATATGCA CTGTAATCGG   241 AAATGTTAAT AGATTTTGTG GGTGCTCCCT GAGGTGCTAT AGCTTGCAGT GGGCCAGCAC   301 CCTGATACAG TGTTTTGGAT GTTCGAGAGA TGGCACCGAA TCGAGAGACT GTTGGTCGGT   361 CTCCAAACGG GATGAGGTCA TCATCGCTCG TCTCCACTGC TCTTCTCTGA AGGTCCAACC   421 TCTGCTCTCT CGTTCCTTTA CTTTCTACAT TCATCATTTC CACACTCCCC GCTGCCACAA   481 CATCTTTGGG TTTTGCATCT TTGGTTCCAA TGTAGGAGCC GATGGTATCA CATGACCAAG   541 GAGAGTACTG GCTATAATCA TTTGCTTTGT ATTTTCCGGC TACCTTCAAG TCTTCATCCA   601 AAAATTCCTC TGGATGGAAT GCAGGAGGCA ATGTTGGTGA CGGTGCAAAA GGAGGTGGAG   661 AGATGACTTT TCTTTCCTCT AGCTGGGCCA TTATTTCCTT CCGCCTTCGA TGTAGCTCAT   721 CCAGACTAGG ATGGGGTTGG GGAGGANGAA GCCGACTATA ANGAGGNTCC CTGGGGTATG   781 AANGTCTGAT CTGANCTGGA NGANGAGCCA CCGGGTANTA ACCCTCTACC TGTTGGTACC   841 GTTCTCTGGA CTCANGTACA TANGACGGCA CTGCTGCAGA TGGAATGTCN ATGGGTATAN   901 GGNTTTCCCG GAACATCTCC TCTCTTGTGT AANANTGANC NNGGTATACN CNGNNGCNNT   961 CNNANNGNNC TGGGNACATN NNTGGNTACT GTTGNGGCTN NNNGCCNTAC NNNNANTTNA 1021 TNNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, T7 pro, ori Sequence file: RDB18151_B1Alj.seq
>D06974A4_B1Al_3_SV40pro_ori_F_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNGNCNCCT CGNCCTCTGA NCTANTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCT CGAGGAACTG AAAAACCAGA AAGTTAATTC   121 CCTATAGTGA GTCGTATTAA ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT   181 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT   241 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG   301 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG   361 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG   421 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT   481 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC   541 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC   601 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA   661 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT   721 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTANGTATCT CAGTTCGGTG   781 TANGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC NGACCGCTGC   841 GCCTTATCCG GTAACTATCG TCTNGANTNN NACCCGGTAA GANACGACTN ATCGCCACTG   901 GCAGCAGCCN CNGGNANCAG GNNTANCANA NCGAGNNNTG NNNNNNGNGC TNCNNNNTNC 961 NNGNNNNNNN NGNCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl