Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFLAG-mRoquin-1-WT (#RDB18151)

Expression vector of mouse Roquin-1 (Rc3h1).

Clone info. Expression vector of mouse RING CCCH (C3H) domains 1 (Rc3h1; a.k.a. Roquin-1) tagged with FLAG at N-terminus, CMV promoter.
Comment The cDNAs of mouse Roquin-1 (Rc3h1) was kindly provided by Georg Stoecklin (German Cancer Research Center). Expression was confirmed by the depositor with western blotting. This clone contains a cDNA fragment corresponding 443nt to 3831nt of NM_001024952.2. Known difference : substitution A to G at 1285nt (Glu, synonym) ; substitution T to C at 2863nt (Tyr, synonym).
Vector backbone pFLAG-CMV2 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name mouse Rc3h1 cDNA
Depositor|Developer Takeuchi, Osamu | Mino, Takashi |
 
Sequence (full) RDB18151zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18151 pFLAG-mRoquin-1-WT DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-mRoquin-1-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18151).

Reference section:

Mino, T., Iwai, N., Endo, M., Inoue, K., Akaki, K., Hia, F., Uehata, T., Emura, T., Hidaka, K., Suzuki, Y., Standley, D.M., Okada-Hatakeyama, M., Ohno, S., Sugiyama, H., Yamashita, A., Takeuchi, O., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18151_B1Alp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB18151_B1Alf.seq check
>D06974A4_B1Al_3_CMV_Forward_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNCCNTNNNN NCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG
  121 ACAAGCTTGC GGCCGCGCCT GTACAAGCTC CACAATGGAC GGATTTCCTT TCCTGCCCAA
  181 TTTGCACTCA GACTTTCGAC GAAACAATTC GAAAGCCCAT CAGTTTGGGC TGTGGCCATA
  241 CTGTCTGCAA AATGTGCCTG AATAAACTCC ACCGTAAGGC TTGCCCATTT GACCAGACCA
  301 CTATCAATAC GGACATTGAG CTTCTTCCTG TGAACTCAGC ATTACTGCAG CTAGTGGGTG
  361 CTCAGATCCC TGAGCAACAG CCCATTACTT TGTGTAGTGG GGTTGAAGAT ACAAAGCATT
  421 ATGAGGAAGC CAAGAAATGT GTAGAAGAAT TAGCATTGTA CCTGAAGCCA CTCAGCAGTG
  481 CGAGAGGTGT GGGTCTAAAT AGCACTACTC AGAGTGTTCT GAGTCGCCCA ATGCAGAGGA
  541 AACTTGTGAC TCTGGTTCAT TGCCAGCTAG TGGAAGAAGA AGGCAGGATC CGTGCTATGA
  601 GGGCTGCTCG ATCTTTAGGT GAACGCACAG TTACAGAGCT CATTCTCCAG CACCAGAATC
  661 CTCAGCAACT CTCTTCTAAT CTCTGGGCAG CAGTCAGAGC TAGGGGCTGC CAGTTCCTTG
  721 GACCAGCGAT GCAGGAGGAA GCTCTGAAGC TGGTCTTGCT GGCCTTAGAA GATGGTTCTG
  781 CATTGTCTCG GAAAGTGTTG GTTCTCTTCG TGGTGCAAAG ACTGGANCCA CGTTTTCCTC
  841 AAGCCTCTAA AACCAGCATT GNGCATGTTG NTCAGCTCCT CTACAGANCC TCCTGCTNCN
  901 AGGTCANNNA ACGTGATGAA NANTCTTCTT TGATGCANCT GAAANAANAA TTTANNNNTT
  961 ACNANNCTCT GANGANANAG CATGANNNCN NNNNANNGNN AANNGNTANN GNANNCTNGN
1021 NNNNNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), insert 3'
Sequence file: RDB18151_B1Alg.seq check
>D06974A4_B1Al_3_hGHpolyA-R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN AANANTATAG AAGGNNACCT AGTCAGACAA AATGATGCAA
   61 CTTAATTTTA TTAGGACAAG GCTGGTGGGC ACTGGAGTGG CAACTTCCAG GGCCAGGAGA
  121 GGCACTGGGG AGGGGTCACA GGGATGCCAC CCGGGATCCT CTAGAGTCGA CTGGTACCGA
  181 TATCAGATCT ATCGATGAAT TCTTAGGGAG CAGAATTGGA AACAACTCCT GATCTCTGGG
  241 AATCATTGTT ATCTCCTCCT CCCTCAGAGT CTTCTGACAG ATTCAAAGAG CTGGTCTTGT
  301 TTGATAGGAG GCTGAGATTC TCTTGTGTCA AGGCTGATCC ATTTGGTACA TCACTGAATG
  361 TCAATGTAAG GTCCTCAGTG CGGATCTTGT TTTGGGGCTC GGGCTGGCCA TTTTCTGCTT
  421 GCTTGCTTGT ACCCAGTTTG CTTTTCATGT CTACAGAACA CTGGTTCTCC ATACTCAATT
  481 CCCGGGTCCT CTTCCCAATT TCTCTTTCCA CCTGATGCAG TTCCAAGCTG AGCTGCTCAC
  541 TTGAGATCAT TCCAGGCCAT TTTGGAGGCA GCTGAGGGGG CTGTGGCTGG CTTGCCAGGG
  601 TGTTCACCTC CCTCTGTAAC ACCAGCCGGT TACTAACAGC CTCTAGTCCT CGTAGCTGGG
  661 TCTGCTGGCT GATCTGATGG TTCAGCTGCT GCAATTCTAG CCGTAATTGT TCTCTTTCAG
  721 CAGATAGAAN GGGTTTTCCA TGACTGGCCA CTTCTGCCAT GGACATTTTC TCCCTCTCAA
  781 TGAAGTGTCC CTGANGANGT ATGTTTGCAT GANGTGAGTA TGGAGAATCT CCCCAGCCAC
  841 CATGAGCTCC ATATGCACTG TAATCGGAAN TGTTAATAGA TTTTGTGGGT GCTCCCTGAN
  901 GTGCTATAGC TTGCAGTGGG CCANCACCCT GATACAGTGT TTTNNANGNT CNANANANGG
  961 NNCNNNNTCN AGANNNTGNT NNNCNGNCTN CNAACNGNNN GNNNNCNTCN TCNNNCGNNN
1021 CCNNNGNNNN NCNNNNNNNN NN
//
Primer: mRoquin-M_F1 (Pr0856)
Region: insert mid
Sequence file: RDB18151_B1Alh.seq check
>D06974A4_B1Al_3_mRoquin-M_F1_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNNNTCTGGT TNNTTGCCNG CTAGTGGAAG AAGAAGGNAG
   61 GATCCGTGCT ATGAGGGCTG CTCGATCTTT AGGTGAACGC ACAGTTACAG AGCTCATTCT
  121 CCAGCACCAG AATCCTCAGC AACTCTCTTC TAATCTCTGG GCAGCAGTCA GAGCTAGGGG
  181 CTGCCAGTTC CTTGGACCAG CGATGCAGGA GGAAGCTCTG AAGCTGGTCT TGCTGGCCTT
  241 AGAAGATGGT TCTGCATTGT CTCGGAAAGT GTTGGTTCTC TTCGTGGTGC AAAGACTGGA
  301 GCCACGTTTT CCTCAAGCCT CTAAAACCAG CATTGGGCAT GTTGTTCAGC TCCTCTACAG
  361 AGCCTCCTGC TTCAAGGTCA CCAAACGTGA TGAAGACTCT TCTTTGATGC AACTGAAAGA
  421 AGAATTTAGG ACTTACGAAG CTCTGAGGAG AGAGCATGAC TCCCAGATAG TGCAAATTGC
  481 TATGGAAGCT GGCTTAAGGA TTGCACCAGA CCAGTGGTCC TCTTTGCTTT ATGGGGATCA
  541 GTCTCACAAA TCTCATATGC AGTCTATTAT TGACAAGTTA CAGACCCCTG CCTCATTTGC
  601 ACAGAGTGTT CAGGAGCTAA CAATTGCTCT CCAGCGGACT GGAGACCCAG CGAATTTGAA
  661 CCGACTAAGA CCCCATTTGG AGTTACTCGC AAACATTGAC CCTAGTCCAG ATGCTCCTCC
  721 TCCAACATGG GAACAGCTAG AAAATGGGCT GGTTGCTGTA CGTACGGTAG TGCATGGATT
  781 GGTTGANTAT ATCCAGAACC ACAGCAAAAA AGGAGCAGAT CAACNACAGC CTCCACAGCA
  841 TANCAAATAC AAAACATACA TGTGTCGAGA TANGAAGCAA ANGGNANGAN GNCCTCNNGG
  901 TGNCAGCTGT ACGTTTGCAC ATTCNCANGA ANAANTNNAA AANTTTCGNA AAANGNANNA
  961 ACGTCTGGNT CCNNNANNAN CNTTGNNNGN NNCNTNNGGN CNNCTTNNTG NNNNGGNNCN
1021 GNCNNNNNNN NNN
//
Primer: mRoquin-M_R1 (Pr0857)
Region: insert mid
Sequence file: RDB18151_B1Ali.seq check
>D06974A4_B1Al_3_mRoquin-M_R1_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNCNNCNAGT CNTCGTAGCT GGGTCTGCTG GCTGATCTGA TGGTTCAGCT
   61 GCTGCAATTC TAGCCGTAAT TGTTCTCTTT CAGCAGATAG AAGGGGTTTT CCATGACTGG
  121 CCACTTCTGC CATGGACATT TTCTCCCTCT CAATGAAGTG TCCCTGAGGA GGTATGTTTG
  181 CATGAGGTGA GTATGGAGAA TCTCCCCAGC CACCATGAGC TCCATATGCA CTGTAATCGG
  241 AAATGTTAAT AGATTTTGTG GGTGCTCCCT GAGGTGCTAT AGCTTGCAGT GGGCCAGCAC
  301 CCTGATACAG TGTTTTGGAT GTTCGAGAGA TGGCACCGAA TCGAGAGACT GTTGGTCGGT
  361 CTCCAAACGG GATGAGGTCA TCATCGCTCG TCTCCACTGC TCTTCTCTGA AGGTCCAACC
  421 TCTGCTCTCT CGTTCCTTTA CTTTCTACAT TCATCATTTC CACACTCCCC GCTGCCACAA
  481 CATCTTTGGG TTTTGCATCT TTGGTTCCAA TGTAGGAGCC GATGGTATCA CATGACCAAG
  541 GAGAGTACTG GCTATAATCA TTTGCTTTGT ATTTTCCGGC TACCTTCAAG TCTTCATCCA
  601 AAAATTCCTC TGGATGGAAT GCAGGAGGCA ATGTTGGTGA CGGTGCAAAA GGAGGTGGAG
  661 AGATGACTTT TCTTTCCTCT AGCTGGGCCA TTATTTCCTT CCGCCTTCGA TGTAGCTCAT
  721 CCAGACTAGG ATGGGGTTGG GGAGGANGAA GCCGACTATA ANGAGGNTCC CTGGGGTATG
  781 AANGTCTGAT CTGANCTGGA NGANGAGCCA CCGGGTANTA ACCCTCTACC TGTTGGTACC
  841 GTTCTCTGGA CTCANGTACA TANGACGGCA CTGCTGCAGA TGGAATGTCN ATGGGTATAN
  901 GGNTTTCCCG GAACATCTCC TCTCTTGTGT AANANTGANC NNGGTATACN CNGNNGCNNT
  961 CNNANNGNNC TGGGNACATN NNTGGNTACT GTTGNGGCTN NNNGCCNTAC NNNNANTTNA
1021 TNNNNNNNNN N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, T7 pro, ori
Sequence file: RDB18151_B1Alj.seq check
>D06974A4_B1Al_3_SV40pro_ori_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNGNCNCCT CGNCCTCTGA NCTANTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCT CGAGGAACTG AAAAACCAGA AAGTTAATTC
  121 CCTATAGTGA GTCGTATTAA ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT
  181 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
  241 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
  301 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
  361 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
  421 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
  481 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
  541 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
  601 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
  661 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
  721 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTANGTATCT CAGTTCGGTG
  781 TANGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC NGACCGCTGC
  841 GCCTTATCCG GTAACTATCG TCTNGANTNN NACCCGGTAA GANACGACTN ATCGCCACTG
  901 GCAGCAGCCN CNGGNANCAG GNNTANCANA NCGAGNNNTG NNNNNNGNGC TNCNNNNTNC
961 NNGNNNNNNN NGNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mino, T., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]