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pFLAG-mRegnase-1-KR257258AA (KRAA) (#RDB18150)

Expression vector of mouse zinc finger protein Regnase-1 (Zc3h12a) with K257A/R258A (KRAA) mutation.

Clone info. Expression vector of mouse zinc finger CCCH type containing 12A (Zc3h12a; a.k.a. Regnase-1) with K257A/R258A (KRAA) mutation, tagged with FLAG at N-terminus, CMV promoter. Mutation of one of the four regions potentially recognizable on the surface of the Regnase-1 RNase domain, see Fig. S2D and E of Mino, T. et al., Nucleic Acids Res., 47 (16): 8838-8859, 2019.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pFLAG-CMV2 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name mouse Zc3h12a cDNA
Depositor|Developer Takeuchi, Osamu | Mino, Takashi |
 
Sequence (full) RDB18150zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18150 pFLAG-mRegnase-1-KR257258AA (KRAA) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pFLAG-mRegnase-1-KR257258AA (KRAA) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18150).

Reference section:

Mino, T., Iwai, N., Endo, M., Inoue, K., Akaki, K., Hia, F., Uehata, T., Emura, T., Hidaka, K., Suzuki, Y., Standley, D.M., Okada-Hatakeyama, M., Ohno, S., Sugiyama, H., Yamashita, A., Takeuchi, O., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Mino, T., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]