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pFLAG-mRegnase-1-WT (#RDB18148)

Expression vector of mouse zinc finger protein Regnase-1 (Zc3h12a).

Clone info. Expression vector of mouse zinc finger CCCH type containing 12A (Zc3h12a; a.k.a. Regnase-1) tagged with FLAG at N-terminus, CMV promoter.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pFLAG-CMV2 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Amp^r
Gene/insert name mouse Zc3h12a cDNA
Depositor|Developer Takeuchi, Osamu | Mino, Takashi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18148 pFLAG-mRegnase-1-WT DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFLAG-mRegnase-1-WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18148).

Reference section:

Mino, T., Iwai, N., Endo, M., Inoue, K., Akaki, K., Hia, F., Uehata, T., Emura, T., Hidaka, K., Suzuki, Y., Standley, D.M., Okada-Hatakeyama, M., Ohno, S., Sugiyama, H., Yamashita, A., Takeuchi, O., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18148_B1Alp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB18148_B1Ala.seq check
>D06974A1_B1AI_1_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG
  121 ACAAGCTTGC GGCCGCGAGT GACCCTTGTG GAACGAAGCC TGTCCAAGAA TCCAACCCCA
  181 CCATGAGTCT GTGGAGTCTT GAGGACAGAC ACAGCAGCCA GGGTCGACCT CAGCCAGACC
  241 AGGATCCTGT GGCTAAAGAG GCCCCTACTT CCGAGCTGCA GATGAAGGTG GACTTTTTCC
  301 GTAAACTGGG GTACTCGTCC TCTGAGATCC ACAGTGTCCT GCAGAAGCTG GGAGTCCAAG
  361 CAGACACCAA CACGGTGCTA GGGGAATTGG TGAAGCATGG CTCAGCTACT GAACGAGAAT
  421 GCCAGGCCCT GACGGCCCCC AGCCCCCAGC CCCCTCTGGT GCCCCGGGGT GGAAGCACCC
  481 CCAAGCCTTC CACTCTAGAA CCCTCACTCC CAGAGGAGGA CAGAGAGGGC AGCGACCTGA
  541 GACCTGTGGT CATCGACGGA AGCAATGTGG CCATGAGCCA TGGGAACAAG GAAGTCTTCT
  601 CTTGCCGGGG CATTCTGCTG GCTGTGAACT GGTTTCTGGA GCGAGGCCAC ACAGATATTA
  661 CCGTGTTTGT GCCATCTTGG AGGAAGGAAC AGCCTCGACC AGATGTGCCT ATCACAGACC
  721 AGCACATCCT TCGGGAACTA GAGAAAAAGA AGATCTTGGT GTTCACGCCA TCCAGGCGGG
  781 TCGGCGGCAA GCGCGTGGTG TGCTATGATG ACCGCTTCAT TGTGAAGCTG GNCTTCNAAT
  841 CCGACGGANT GGTGGTCTCC AATGACACGT ACCGAGACNT CCNANGCGAG AGGCANGAGT
  901 GGAAACGCTT CATCGAGGAN CGGCTGCTNN TGTNNTCCTT CGNCAATGAN NAGTNCATGN
961 CCCCNNNNNN ANCNNTTNNN ANGGCATGGG NCNTANNNNN NNNNN
//
Primer: mZc3h12a-M_R1 (Pr0855)
Region: insert mid
Sequence file: RDB18148_B1Alb.seq check
>D06974A1_B1AI_1_mZc3h12a-M_R1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNGNCNN TTGNNTCCNT ACGTACATTT CTTCCCNTAG
   61 GGGCATGGCT GCTTCCTGTG CTCAGAAGGC AGTGGTTTCT TACGAAGGAA GTTGTCCAGG
  121 CTAGGCCCAT GCCGTCCTAA AGGGTCGTCA GGGGGCATGA ACTTGTCATT GACGAAGGAG
  181 TACATGAGCA GCCGCTCCTC GATGAAGCGT TTCCACTCCT GCCTCTCGCC TTGGAGGTCT
  241 CGGTACGTGT CATTGGAGAC CACCACTCCG TCGGATTCGA AGGCCAGCTT CACAATGAAG
  301 CGGTCATCAT AGCACACCAC GCGCTTGCCG CCGACCCGCC TGGATGGCGT GAACACCAAG
  361 ATCTTCTTTT TCTCTAGTTC CCGAAGGATG TGCTGGTCTG TGATAGGCAC ATCTGGTCGA
  421 GGCTGTTCCT TCCTCCAAGA TGGCACAAAC ACGGTAATAT CTGTGTGGCC TCGCTCCAGA
  481 AACCAGTTCA CAGCCAGCAG AATGCCCCGG CAAGAGAAGA CTTCCTTGTT CCCATGGCTC
  541 ATGGCCACAT TGCTTCCGTC GATGACCACA GGTCTCAGGT CGCTGCCCTC TCTGTCCTCC
  601 TCTGGGAGTG AGGGTTCTAG AGTGGAAGGC TTGGGGGTGC TTCCACCCCG GGGCACCAGA
  661 GGGGGCTGGG GGCTGGGGGC CGTCAGGGCC TGGCATTCTC GTTCAGTAGC TGAGCCATGC
  721 TTCACCAATT CCCCTAGCAC CGTGTTGGTG TCTGCTTGGA CTCCCAGCTT CTGCAGGACA
  781 CTGTGGATCT CAGANGACGA GTACCCCAGT TTACGGAAAA AGTCCACCTT CATCTGCAGC
  841 TCGGAAGTAN GGGCCTCTTT AGCCACAGGA TCCTGGTCTG GNTGANGTCG ANCCTGGCTG
  901 CTGTGTCTGT CCTCNAGANT CNNCAGACTC NTGGNGGGGT TGGANTCNNG GACAGGNNNC
  961 NTTCCNNNAN NGNTCNNTCN NGGNNNCNAG CTNGNCNNCA TCNNNNTNGN ANNNCNNNNN
1021 NNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: hGH p(A), insert 3'
Sequence file: RDB18148_B1Alc.seq check
>D06974A1_B1AI_1_hGHpolyA-R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNCCNNNNN NANTATAGAA GGNNNCCTAG TCNGACAAAA TGATGCAACT
   61 TAATTTTATT AGGACAAGGC TGGTGGGCAC TGGAGTGGCA ACTTCCAGGG CCAGGAGAGG
  121 CACTGGGGAG GGGTCACAGG GATGCCACCC GGGATCCTCT AGAGTCGACT GGTACCGATA
  181 TCAGATCTAT CGATGAATTC TTACTCACTG AGGTGCTGGG ACTTGTAAGA CAGGATCTCT
  241 GCGGCCAGCT GCTGCGGATC CAGCAGCTGT GGGAAGCGTC TCATTACAGC TTCTACCAGG
  301 TGTGGGGGGA AGACACCACA CAGCTTGGTA TATACACCAG CCCTTTCTTT GGCCAGGTCA
  361 CCCACCCTGC CCCAGGGGCC CCCACCAGCC CCGGGGCTGG GTCTCTGGGG CTCCTGAAGG
  421 ACAGGAGTGG GTGGGGGTAA TGGGTACGGC TCAGACCAGT ACTCTCTGGA TGGGTAGGTG
  481 GGTGGCGGGG GCACATAGTC GGTGGGGACA CTGAAGTGGC CAGCACCCAT GGCTGGTCTA
  541 AAAGGAGAAA AGGAAGGTGC TGCTGGGACC TTGGATCCAT AGCTGTGATA ACCTGCATAG
  601 GGGCCCCGAG TGGGGCCCGA CTCCCCAGGG CTGCCTCCTC GCACGCCCCA TAATTCTGAC
  661 ATCTGGCTCT CCAGGGAACC AATGCCTGAA TCAAGGCACT CCTGGGANGT GTATGGGAGT
  721 GAGTCCTGGG TGTGTGCAAG CCACTCTGTG GGCCCNAAGC TGCCCCCACT AACAGGGANG
  781 CTCCTGCCAG CTGGANCACA NGTCTGCGGT GANCCTTCTC GGTGGGGACC CGGANANGAN
  841 CTGNCACCCA GCTTTTTNCC NTCCNGGCTG CCTGGNTCAG CCTCNANGGN NACAGANCTG
  901 GNNTGANANG CANGGGAANG NCTCTGGNTA CTTTTGNCCT TNNNNGNNNT CCTGNNNNGT
961 GANANGANAN CGTTGGNNNN GNNCNCATCN GNNNNCNNNN CNNTGGGNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro, ori
Sequence file: RDB18148_B1Ald.seq check
>D06974A1_B1AI_1_SV40pro_ori_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGCC TCNNCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGAGGAACT GAAAAACCAG AAAGTTAATT
  121 CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG
  181 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
  241 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
  301 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
  361 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
  421 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
  481 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
  541 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
  601 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
  661 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
  721 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTANGTATC TCAGTTCGGT
  781 GTANGTCGTT CGCTCCNAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
  841 CGCCTTATCC GGTAACTATC GTCTTGANNC CNACCCGGTA AGANNCGACN NATCGCCNCN
  901 GGCAGCAGCC NCNNGTAACA GGNNTANCAN ANCGNNGNAN GTANNNNGNG CTNCNNANTN
961 CNNGNNNNNN NGNNNNNANT ANNNNNANNN TANNNNNNGT ATTNGGNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Mino, T., Translation-dependent unwinding of stem-loops by UPF1 licenses Regnase-1 to degrade inflammatory mRNAs. Nucleic Acids Res. 47 (16): 8838-8859 (2019). PMID 31329944. [link to RRC of NBRP]