Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFLAG-mRegnase-1-WT

Expression vector of mouse zinc finger protein Regnase-1 (Zc3h12a).

Distribution information

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Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mino, T. et al., Nucleic Acids Res. 47 (16), 8838-8859, 2019) 。学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18148_B1Alp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, FLAG, insert 5' Sequence file: RDB18148_B1Ala.seq
>D06974A1_B1AI_1_CMV_Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCG TTTAGTGAAC CGTCAGAATT GATCTACCAT GGACTACAAA GACGATGACG   121 ACAAGCTTGC GGCCGCGAGT GACCCTTGTG GAACGAAGCC TGTCCAAGAA TCCAACCCCA   181 CCATGAGTCT GTGGAGTCTT GAGGACAGAC ACAGCAGCCA GGGTCGACCT CAGCCAGACC   241 AGGATCCTGT GGCTAAAGAG GCCCCTACTT CCGAGCTGCA GATGAAGGTG GACTTTTTCC   301 GTAAACTGGG GTACTCGTCC TCTGAGATCC ACAGTGTCCT GCAGAAGCTG GGAGTCCAAG   361 CAGACACCAA CACGGTGCTA GGGGAATTGG TGAAGCATGG CTCAGCTACT GAACGAGAAT   421 GCCAGGCCCT GACGGCCCCC AGCCCCCAGC CCCCTCTGGT GCCCCGGGGT GGAAGCACCC   481 CCAAGCCTTC CACTCTAGAA CCCTCACTCC CAGAGGAGGA CAGAGAGGGC AGCGACCTGA   541 GACCTGTGGT CATCGACGGA AGCAATGTGG CCATGAGCCA TGGGAACAAG GAAGTCTTCT   601 CTTGCCGGGG CATTCTGCTG GCTGTGAACT GGTTTCTGGA GCGAGGCCAC ACAGATATTA   661 CCGTGTTTGT GCCATCTTGG AGGAAGGAAC AGCCTCGACC AGATGTGCCT ATCACAGACC   721 AGCACATCCT TCGGGAACTA GAGAAAAAGA AGATCTTGGT GTTCACGCCA TCCAGGCGGG   781 TCGGCGGCAA GCGCGTGGTG TGCTATGATG ACCGCTTCAT TGTGAAGCTG GNCTTCNAAT   841 CCGACGGANT GGTGGTCTCC AATGACACGT ACCGAGACNT CCNANGCGAG AGGCANGAGT   901 GGAAACGCTT CATCGAGGAN CGGCTGCTNN TGTNNTCCTT CGNCAATGAN NAGTNCATGN 961 CCCCNNNNNN ANCNNTTNNN ANGGCATGGG NCNTANNNNN NNNNN //
Primer: mZc3h12a-M_R1 (Pr0855) Region: insert mid Sequence file: RDB18148_B1Alb.seq
>D06974A1_B1AI_1_mZc3h12a-M_R1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNGNCNN TTGNNTCCNT ACGTACATTT CTTCCCNTAG    61 GGGCATGGCT GCTTCCTGTG CTCAGAAGGC AGTGGTTTCT TACGAAGGAA GTTGTCCAGG   121 CTAGGCCCAT GCCGTCCTAA AGGGTCGTCA GGGGGCATGA ACTTGTCATT GACGAAGGAG   181 TACATGAGCA GCCGCTCCTC GATGAAGCGT TTCCACTCCT GCCTCTCGCC TTGGAGGTCT   241 CGGTACGTGT CATTGGAGAC CACCACTCCG TCGGATTCGA AGGCCAGCTT CACAATGAAG   301 CGGTCATCAT AGCACACCAC GCGCTTGCCG CCGACCCGCC TGGATGGCGT GAACACCAAG   361 ATCTTCTTTT TCTCTAGTTC CCGAAGGATG TGCTGGTCTG TGATAGGCAC ATCTGGTCGA   421 GGCTGTTCCT TCCTCCAAGA TGGCACAAAC ACGGTAATAT CTGTGTGGCC TCGCTCCAGA   481 AACCAGTTCA CAGCCAGCAG AATGCCCCGG CAAGAGAAGA CTTCCTTGTT CCCATGGCTC   541 ATGGCCACAT TGCTTCCGTC GATGACCACA GGTCTCAGGT CGCTGCCCTC TCTGTCCTCC   601 TCTGGGAGTG AGGGTTCTAG AGTGGAAGGC TTGGGGGTGC TTCCACCCCG GGGCACCAGA   661 GGGGGCTGGG GGCTGGGGGC CGTCAGGGCC TGGCATTCTC GTTCAGTAGC TGAGCCATGC   721 TTCACCAATT CCCCTAGCAC CGTGTTGGTG TCTGCTTGGA CTCCCAGCTT CTGCAGGACA   781 CTGTGGATCT CAGANGACGA GTACCCCAGT TTACGGAAAA AGTCCACCTT CATCTGCAGC   841 TCGGAAGTAN GGGCCTCTTT AGCCACAGGA TCCTGGTCTG GNTGANGTCG ANCCTGGCTG   901 CTGTGTCTGT CCTCNAGANT CNNCAGACTC NTGGNGGGGT TGGANTCNNG GACAGGNNNC   961 NTTCCNNNAN NGNTCNNTCN NGGNNNCNAG CTNGNCNNCA TCNNNNTNGN ANNNCNNNNN 1021 NNNNN //
Primer: hGHpolyA-R (Pr0350) Region: hGH p(A), insert 3' Sequence file: RDB18148_B1Alc.seq
>D06974A1_B1AI_1_hGHpolyA-R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNCCNNNNN NANTATAGAA GGNNNCCTAG TCNGACAAAA TGATGCAACT    61 TAATTTTATT AGGACAAGGC TGGTGGGCAC TGGAGTGGCA ACTTCCAGGG CCAGGAGAGG   121 CACTGGGGAG GGGTCACAGG GATGCCACCC GGGATCCTCT AGAGTCGACT GGTACCGATA   181 TCAGATCTAT CGATGAATTC TTACTCACTG AGGTGCTGGG ACTTGTAAGA CAGGATCTCT   241 GCGGCCAGCT GCTGCGGATC CAGCAGCTGT GGGAAGCGTC TCATTACAGC TTCTACCAGG   301 TGTGGGGGGA AGACACCACA CAGCTTGGTA TATACACCAG CCCTTTCTTT GGCCAGGTCA   361 CCCACCCTGC CCCAGGGGCC CCCACCAGCC CCGGGGCTGG GTCTCTGGGG CTCCTGAAGG   421 ACAGGAGTGG GTGGGGGTAA TGGGTACGGC TCAGACCAGT ACTCTCTGGA TGGGTAGGTG   481 GGTGGCGGGG GCACATAGTC GGTGGGGACA CTGAAGTGGC CAGCACCCAT GGCTGGTCTA   541 AAAGGAGAAA AGGAAGGTGC TGCTGGGACC TTGGATCCAT AGCTGTGATA ACCTGCATAG   601 GGGCCCCGAG TGGGGCCCGA CTCCCCAGGG CTGCCTCCTC GCACGCCCCA TAATTCTGAC   661 ATCTGGCTCT CCAGGGAACC AATGCCTGAA TCAAGGCACT CCTGGGANGT GTATGGGAGT   721 GAGTCCTGGG TGTGTGCAAG CCACTCTGTG GGCCCNAAGC TGCCCCCACT AACAGGGANG   781 CTCCTGCCAG CTGGANCACA NGTCTGCGGT GANCCTTCTC GGTGGGGACC CGGANANGAN   841 CTGNCACCCA GCTTTTTNCC NTCCNGGCTG CCTGGNTCAG CCTCNANGGN NACAGANCTG   901 GNNTGANANG CANGGGAANG NCTCTGGNTA CTTTTGNCCT TNNNNGNNNT CCTGNNNNGT 961 GANANGANAN CGTTGGNNNN GNNCNCATCN GNNNNCNNNN CNNTGGGNNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro, ori Sequence file: RDB18148_B1Ald.seq
>D06974A1_B1AI_1_SV40pro_ori_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNGNNGCC TCNNCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGAGGAACT GAAAAACCAG AAAGTTAATT   121 CCCTATAGTG AGTCGTATTA AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG   181 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG   241 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC   301 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT   361 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT   421 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA   481 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC   541 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG   601 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG   661 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT   721 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTANGTATC TCAGTTCGGT   781 GTANGTCGTT CGCTCCNAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG   841 CGCCTTATCC GGTAACTATC GTCTTGANNC CNACCCGGTA AGANNCGACN NATCGCCNCN   901 GGCAGCAGCC NCNNGTAACA GGNNTANCAN ANCGNNGNAN GTANNNNGNG CTNCNNANTN 961 CNNGNNNNNN NGNNNNNANT ANNNNNANNN TANNNNNNGT ATTNGGNANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl