RDB18147 - PHM802

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

PHM802

Integration plasmid to Ty1 loci. Recognized by the gRNA #4. CRISPR/Transposon gene integration (CRITGI) gene expression technology clone.

Catalog number	RDB18147
Resource name	PHM802
Clone info.	The gene expression technology clone, CRITGI (CRISPR/Transposon gene integration). Integration vector harboring S. cerevisiae short Ty1 HR sequence recognized by the gRNA #4, 2xBsaI sites, LEU2 marker in pRS405, Hanasaki, M. and Masumoto, H., Sci. Rep., 9 (1): 15300, 2019.
Vector backbone	pRS405 (plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Ampicillin (E. coli), LEU2 (S. cerevisiae)
Growth remarks	not specify
Gene/insert name	S. cerevisiae Ty1 HR genomic DNA
Depositor\|Developer	Masumoto, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hanasaki, M. et al., Sci. Rep. 9 (1), 15300, 2019)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18147	PHM802	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB18147zzk01.seq

Original reference

original	Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Saccharomyces cerevisiae (English text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Hanasaki, M., CRISPR/Transposon gene integration (CRITGI) can manage gene expression in a retrotransposon-dependent manner. Sci. Rep. 9 (1): 15300 (2019). PMID 31653950.

2020.10.26

GNP_filter3_RDBDEP_html_200316.pl